XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent

Zhang, Nan; Dong, Xiaoou; Jain, Rashmi; Ruan, Deling; de Araujo Junior, Artur Teixeira; Li, Yan; Lipzen, Anna; Martin, Joel; Barry, Kerrie; Ronald, Pamela C.

doi:10.7717/peerj.17323

Title: XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent

Journal Article · 05 May 2024 · PeerJ

DOI: https://doi.org/10.7717/peerj.17323 · OSTI ID:2407029

Zhang, Nan ^[1]; Dong, Xiaoou ^[2]; Jain, Rashmi ^[3]; Ruan, Deling ^[4]; de Araujo Junior, Artur Teixeira ^[3]; Li, Yan ^[5]; Lipzen, Anna ^[6]; Martin, Joel ^[6]; Barry, Kerrie ^[6]; Ronald, Pamela C. ^[7]

Chinese Academy of Sciences (CAS), Beijing (China). Institute of Genetics and Developmental Biology; Univ. of California, Davis, CA (United States)
Univ. of California, Davis, CA (United States); Nanjing Agricultural University (China); Univ. of California, Berkeley, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)
Univ. of California, Davis, CA (United States)
Univ. of California, Davis, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)
Univ. of California, Davis, CA (United States); Sichuan Agricultural University, Chengdu (China)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Univ. of California, Davis, CA (United States); Univ. of California, Berkeley, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)

The rice receptor kinase XA21 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of rice bacterial blight disease. To investigate the relationship between the expression level of XA21 and resulting resistance, we generated independent HA-XA21 transgenic rice lines accumulating the XA21 immune receptor fused with an HA epitope tag. Whole-genome sequence analysis identified the T-DNA insertion sites in sixteen independent T0 events. Further, through quantification of the HA-XA21 protein and assessment of the resistance to Xoo strain PXO99 in six independent transgenic lines, we observed that XA21-mediated resistance is dose dependent. In contrast, based on the four agronomic traits quantified in these experiments, yield is unlikely to be affected by the expression level of HA-XA21. These findings extend our knowledge of XA21-mediated defense and contribute to the growing number of well-defined genomic landing pads in the rice genome that can be targeted for gene insertion without compromising yield.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2407029

Journal Information:: PeerJ, Journal Name: PeerJ Journal Issue: 5 Vol. 12; ISSN 2167-8359

Publisher:: PeerJ Inc.Copyright Statement

Country of Publication:: United States

Language:: English

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Title: XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent

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