Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

McTaggart, Tami; Beck, David; Setboonsarng, Usanisa; Shapiro, Nicole; Woyke, Tanja; Lidstrom, Mary; Kalyuzhnaya, Marina; Chistoserdova, Ludmila

doi:10.3390/microorganisms3010094

Title: Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

Journal Article · Fri Mar 20 00:00:00 UTC 2015 · Microorganisms

DOI: https://doi.org/10.3390/microorganisms3010094 · OSTI ID:2369826

McTaggart, Tami; Beck, David; Setboonsarng, Usanisa; Shapiro, Nicole; Woyke, Tanja; Lidstrom, Mary; Kalyuzhnaya, Marina; Chistoserdova, Ludmila

Gram-positive methylotrophic bacteria have been known for a long period of time, some serving as model organisms for characterizing the specific details of methylotrophy pathways/enzymes within this group. However, genome-based knowledge of methylotrophy within this group has been so far limited to a single species, Bacillus methanolicus (Firmicutes). The paucity of whole-genome data for Gram-positive methylotrophs limits our global understanding of methylotrophy within this group, including their roles in specific biogeochemical cycles, as well as their biotechnological potential. Here, we describe the isolation of seven novel strains of Gram-positive methylotrophs that include two strains of Bacillus and five representatives of Actinobacteria classified within two genera, Arthrobacter and Mycobacterium. We report whole-genome sequences for these isolates and present comparative analysis of the methylotrophy functional modules within these genomes. The genomic sequences of these seven novel organisms, all capable of growth on methylated amines, present an important reference dataset for understanding the genomic basis of methylotrophy in Gram-positive methylotrophic bacteria. This study is a major contribution to the field of methylotrophy, aimed at closing the gap in the genomic knowledge of methylotrophy within this diverse group of bacteria.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; SC0010556

OSTI ID:: 2369826

Journal Information:: Microorganisms, Journal Name: Microorganisms Journal Issue: 1 Vol. 3; ISSN 2076-2607; ISSN MICRKN

Publisher:: MDPI AGCopyright Statement

Country of Publication:: Switzerland

Language:: English

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Title: Genomics of Methylotrophy in Gram-Positive Methylamine-Utilizing Bacteria

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