Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research

Wang, Shuo; Blume, Rostislav Y.; Zhou, Zhi-Wei; Lu, Shaoping; Nazarenus, Tara J.; Blume, Yaroslav B.; Xie, Weibo; Cahoon, Edgar B.; Chen, Ling-Ling; Guo, Liang

doi:10.1186/s13068-024-02466-9

Title: Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research

Journal Article · Wed Jan 31 00:00:00 UTC 2024 · Biotechnology for Biofuels and Bioproducts

DOI: https://doi.org/10.1186/s13068-024-02466-9 · OSTI ID:2311522

Wang, Shuo ^[1]; Blume, Rostislav Y. ^[2]; Zhou, Zhi-Wei ^[3]; Lu, Shaoping ^[4]; Nazarenus, Tara J. ^[5]; Blume, Yaroslav B. ^[2]; Xie, Weibo ^[4]; Cahoon, Edgar B. ^[5]; Chen, Ling-Ling ^[6]; Guo, Liang ^[4]

Huazhong Agricultural University, Wuhan (China); University of Nebraska
National Academy of Sciences of Ukraine, Kiev (Ukraine)
Huazhong Agricultural University, Wuhan (China)
Huazhong Agricultural University, Wuhan (China); Hubei Hongshan Laboratory, Wuhan (China)
University of Nebraska-Lincoln, NE (United States)
Guangxi University, Nanning (China)

Camelina neglecta is a new diploid Brassicaceae species, which has great research value because of its close relationship with the hexaploid oilseed crop Camelina sativa. Here, we report a chromosome-level assembly of C. neglecta with a total length of 210 Mb. By adopting PacBio sequencing and Hi-C technology, the C. neglecta genome was assembled into 6 chromosomes with scaffold N50 of 29.62 Mb. C. neglecta has undergone the whole-genome triplication (γ) shared among eudicots and two whole-genome duplications (α and β) shared by crucifers, but it has not undergone a specific whole-genome duplication event. By synteny analysis between C. neglecta and C. sativa, we successfully used the method of calculating Ks to distinguish the three subgenomes of C. sativa and determined that C. neglecta was closest to the first subgenome (SG1) of C. sativa. Further, transcriptomic analysis revealed the key genes associated with seed oil biosynthesis and its transcriptional regulation, including SAD, FAD2, FAD3, FAE1, ABI3, WRI1 and FUS3 displaying high expression levels in C. neglecta seeds. The high representability of C. neglecta as a model species for Camelina-based biotechnology research has been demonstrated for the first time. In particular, floral Agrobacterium tumefaciens infiltration-based transformation of C. neglecta, leading to overexpression of CvLPAT2, CpDGAT1 and CvFatB1 transgenes, was demonstrated for medium-chain fatty acid accumulation in C. neglecta seed oil. This study provides an important genomic resource and establishes C. neglecta as a new model for oilseed biotechnology research.

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Research Organization:: University of Nebraska, Lincoln, NE (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)

Grant/Contract Number:: SC0023142

OSTI ID:: 2311522

Journal Information:: Biotechnology for Biofuels and Bioproducts, Journal Name: Biotechnology for Biofuels and Bioproducts Journal Issue: 1 Vol. 17; ISSN 2731-3654

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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