Cooperative control of a DNA origami force sensor

Robbins, Ariel; Hildebolt, Hazen; Neuhoff, Michael; Beshay, Peter; Winter, Jessica O.; Castro, Carlos E.; Bundschuh, Ralf; Poirier, Michael G.

doi:10.1038/s41598-024-53841-3

Title: Cooperative control of a DNA origami force sensor

Journal Article · Mon Feb 19 00:00:00 UTC 2024 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-024-53841-3 · OSTI ID:2308870

Robbins, Ariel; Hildebolt, Hazen; Neuhoff, Michael; Beshay, Peter; Winter, Jessica O.; Castro, Carlos E.; Bundschuh, Ralf; Poirier, Michael G.

Abstract Biomolecular systems are dependent on a complex interplay of forces. Modern force spectroscopy techniques provide means of interrogating these forces, but they are not optimized for studies in constrained environments as they require attachment to micron-scale probes such as beads or cantilevers. Nanomechanical devices are a promising alternative, but this requires versatile designs that can be tuned to respond to a wide range of forces. We investigate the properties of a nanoscale force sensitive DNA origami device which is highly customizable in geometry, functionalization, and mechanical properties. The device, referred to as the NanoDyn, has a binary (open or closed) response to an applied force by undergoing a reversible structural transition. The transition force is tuned with minor alterations of 1 to 3 DNA oligonucleotides and spans tens of picoNewtons (pN). The DNA oligonucleotide design parameters also strongly influence the efficiency of resetting the initial state, with higher stability devices (≳10 pN) resetting more reliably during repeated force-loading cycles. Finally, we show the opening force is tunable in real time by adding a single DNA oligonucleotide. These results establish the potential of the NanoDyn as a versatile force sensor and provide fundamental insights into how design parameters modulate mechanical and dynamic properties.

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Research Organization:: The Ohio State Univ., Columbus, OH (United States); The Ohio State University, Columbus, OH (United States)

Sponsoring Organization:: National Cancer Institute; National Institutes of Health (NIH); National Science Foundation (NSF); USDOE; USDOE Office of Science (SC); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0017270

OSTI ID:: 2308870

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 14; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Title: Cooperative control of a DNA origami force sensor

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