Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling

Zhou, X. Edward; Zhang, Yaguang; Yao, Jian; Zheng, Jie; Zhou, Yuxin; He, Qing; Moreno, Javier; Lam, Vinh Q.; Cao, Xiaoman; Sugimoto, Koichi; Vanegas-Cano, Leidy; Kariapper, Leena; Suino-Powell, Kelly; Zhu, Yuanye; Novick, Scott; Griffin, Patrick R.; Zhang, Feng; Howe, Gregg A.; Melcher, Karsten

doi:10.1016/j.xplc.2023.100639

Title: Assembly of JAZ–JAZ and JAZ–NINJA complexes in jasmonate signaling

Journal Article · Wed Jun 14 00:00:00 UTC 2023 · Plant Communications

DOI: https://doi.org/10.1016/j.xplc.2023.100639 · OSTI ID:2229523

Zhou, X. Edward ^[1]; Zhang, Yaguang ^[2]; Yao, Jian ^[3]; Zheng, Jie ^[4]; Zhou, Yuxin ^[5]; He, Qing ^[6]; Moreno, Javier ^[3]; Lam, Vinh Q. ^[4]; Cao, Xiaoman ^[2]; Sugimoto, Koichi ^[7]; Vanegas-Cano, Leidy ^[3]; Kariapper, Leena ^[6]; Suino-Powell, Kelly ^[6]; Zhu, Yuanye ^[8]; Novick, Scott ^[4]; Griffin, Patrick R. ^[4]; Zhang, Feng ^[8]; Howe, Gregg A. ^[3];

^[6]

Van Andel Institute, Grand Rapids, MI (United States); Michigan State University
Nanjing Agricultural University (China)
Michigan State University, East Lansing, MI (United States)
UF Scripps Biomedical Research, Jupiter, FL (United States)
Van Andel Institute, Grand Rapids, MI (United States); Nanjing Agricultural University (China); Jiangsu Province and Chinese Academy of Sciences, Nanjing (China)
Van Andel Institute, Grand Rapids, MI (United States)
Michigan State University, East Lansing, MI (United States); University of Tsukuba (Japan)
Van Andel Institute, Grand Rapids, MI (United States); Nanjing Agricultural University (China)

Jasmonates (JAs) are plant hormones with crucial roles in development and stress resilience. They activate MYC transcription factors by mediating the proteolysis of MYC inhibitors called JAZ proteins. In the absence of JA, JAZ proteins bind and inhibit MYC through the assembly of MYC–JAZ–Novel Interactor of JAZ (NINJA)–TPL repressor complexes. However, JAZ and NINJA are predicted to be largely intrinsically unstructured, which has precluded their experimental structure determination. Through a combination of biochemical, mutational, and biophysical analyses and AlphaFold-derived ColabFold modeling, we characterized JAZ–JAZ and JAZ–NINJA interactions and generated models with detailed, high-confidence domain interfaces. We demonstrate that JAZ, NINJA, and MYC interface domains are dynamic in isolation and become stabilized in a stepwise order upon complex assembly. By contrast, most JAZ and NINJA regions outside of the interfaces remain highly dynamic and cannot be modeled in a single conformation. Our data indicate that the small JAZ Zinc finger expressed in Inflorescence Meristem (ZIM) motif mediates JAZ–JAZ and JAZ–NINJA interactions through separate surfaces, and our data further suggest that NINJA modulates JAZ dimerization. This study advances our understanding of JA signaling by providing insights into the dynamics, interactions, and structure of the JAZ–NINJA core of the JA repressor complex.

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Research Organization:: Michigan State University, East Lansing, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 2229523

Journal Information:: Plant Communications, Journal Name: Plant Communications Journal Issue: 6 Vol. 4; ISSN 2590-3462

Publisher:: Cell PressCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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