Comparison of wild-type KT2440 and genome-reduced EM42 Pseudomonas putida strains for muconate production from aromatic compounds and glucose

Amendola, Caroline R.; Cordell, William T.; Kneucker, Colin M.; Szostkiewicz, Caralyn J.; Ingraham, Morgan A.; Monninger, Michela; Wilton, Rosemarie; Pfleger, Brian F.; Salvachúa, Davinia; Johnson, Christopher W.; Beckham, Gregg T.

doi:10.1016/j.ymben.2023.11.004

Title: Comparison of wild-type KT2440 and genome-reduced EM42 Pseudomonas putida strains for muconate production from aromatic compounds and glucose

Journal Article · Thu Nov 23 00:00:00 UTC 2023 · Metabolic Engineering

DOI: https://doi.org/10.1016/j.ymben.2023.11.004 · OSTI ID:2228468

Amendola, Caroline R. ^[1]; Cordell, William T. ^[2]; Kneucker, Colin M. ^[1]; Szostkiewicz, Caralyn J. ^[1]; Ingraham, Morgan A. ^[1]; Monninger, Michela ^[1]; Wilton, Rosemarie ^[3]; Pfleger, Brian F. ^[4]; Salvachúa, Davinia ^[1]; Johnson, Christopher W. ^[1];

^[1]

National Renewable Energy Laboratory (NREL), Golden, CO (United States). Renewable Resources and Enabling Science Center; Agile BioFoundry, Emeryville, CA (United States)
National Renewable Energy Laboratory (NREL), Golden, CO (United States). Renewable Resources and Enabling Science Center; University of Wisconsin, Madison, WI (United States)
Agile BioFoundry, Emeryville, CA (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)
University of Wisconsin, Madison, WI (United States)

Pseudomonas putida KT2440 is a robust, aromatic catabolic bacterium that has been widely engineered to convert bio-based and waste-based feedstocks to target products. Towards industrial domestication of P. putida KT2440, rational genome reduction has been previously conducted, resulting in P. putida strain EM42, which exhibited characteristics that could be advantageous for production strains. Here, we compared P. putida KT2440-and EM42-derived strains for cis,cis-muconic acid production from an aromatic compound, p-coumarate, and in separate strains, from glucose. To our surprise, the EM42-derived strains did not outperform the KT2440-derived strains in muconate production from either substrate. In bioreactor cultivations, KT2440-and EM42-derived strains produced muconate from p-coumarate at titers of 45 g/L and 37 g/L, respectively, and from glucose at 20 g/L and 13 g/L, respectively. To provide additional insights about the differences in the parent strains, we analyzed growth profiles of KT2440 and EM42 on aromatic compounds as the sole carbon and energy sources. In general, the EM42 strain exhibited reduced growth rates but shorter growth lags than KT2440. We also observed that EM42-derived strains resulted in higher growth rates on glucose compared to KT2440-derived strains, but only at the lowest glucose concentrations tested. Transcriptomics revealed that genome reduction in EM42 had global effects on transcript levels and showed that the EM42-derived strains that produce muconate from glucose exhibit reduced modulation of gene expression in response to changes in glucose concentrations. Overall, our results highlight that additional studies are warranted to understand the effects of genome reduction on microbial metabolism and physiology, especially when intended for use in production strains.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Science (SC), Office of Workforce Development for Teachers & Scientists (WDTS)

Grant/Contract Number:: AC02-06CH11357; AC36-08GO28308; SC0014664

OSTI ID:: 2228468

Report Number(s):: NREL/JA--2A00-87225; MainId:88000; UUID:b5957521-2134-4c7e-9a2c-1c41ddd3a0c0; MainAdminId:71173

Journal Information:: Metabolic Engineering, Journal Name: Metabolic Engineering Vol. 81; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (73)

Minimal genome: Worthwhile or worthless efforts toward being smaller? Choe, Donghui; Cho, Suhyung; Kim, Sun Chang Biotechnology Journal, Vol. 11, Issue 2 https://doi.org/10.1002/biot.201400838	journal	September 2015
Improved Thermotolerance of Genome‐Reduced Pseudomonas putida EM42 Enables Effective Functioning of the P_L/cI857 System Aparicio, Tomás; de Lorenzo, Víctor; Martínez‐García, Esteban Biotechnology Journal, Vol. 14, Issue 1 https://doi.org/10.1002/biot.201800483	journal	November 2018
Limited life cycle and cost assessment for the bioconversion of lignin‐derived aromatics into adipic acid Duuren, Jozef B. J. H.; Wild, Paul J.; Starck, Sören Biotechnology and Bioengineering, Vol. 117, Issue 5 https://doi.org/10.1002/bit.27299	journal	February 2020
Enhancing recombinant protein production with an Escherichia coli host strain lacking insertion sequences Park, Myung Keun; Lee, Sang Hee; Yang, Kyung Seok Applied Microbiology and Biotechnology, Vol. 98, Issue 15 https://doi.org/10.1007/s00253-014-5739-y	journal	April 2014
Industrial biotechnology of Pseudomonas putida: advances and prospects Weimer, Anna; Kohlstedt, Michael; Volke, Daniel C. Applied Microbiology and Biotechnology, Vol. 104, Issue 18 https://doi.org/10.1007/s00253-020-10811-9	journal	August 2020
Engineering genome-reduced Bacillus subtilis for acetoin production from xylose Yan, Panpan; Wu, Yuanqing; Yang, Li Biotechnology Letters, Vol. 40, Issue 2 https://doi.org/10.1007/s10529-017-2481-4	journal	December 2017
A navigation guide of synthetic biology tools for Pseudomonas putida Martin-Pascual, Maria; Batianis, Christos; Bruinsma, Lyon Biotechnology Advances, Vol. 49 https://doi.org/10.1016/j.biotechadv.2021.107732	journal	July 2021
From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions Nikel, Pablo I.; Chavarría, Max; Danchin, Antoine Current Opinion in Chemical Biology, Vol. 34 https://doi.org/10.1016/j.cbpa.2016.05.011	journal	October 2016
Discovery, characterization, and metabolic engineering of Rieske non-heme iron monooxygenases for guaiacol O-demethylation Bleem, Alissa; Kuatsjah, Eugene; Presley, Gerald N. Chem Catalysis, Vol. 2, Issue 8 https://doi.org/10.1016/j.checat.2022.04.019	journal	August 2022
Genome reduction enhances production of polyhydroxyalkanoate and alginate oligosaccharide in Pseudomonas mendocina Fan, Xu; Zhang, Yiting; Zhao, Fengjie International Journal of Biological Macromolecules, Vol. 163 https://doi.org/10.1016/j.ijbiomac.2020.09.067	journal	November 2020
Enhancement of protocatechuate decarboxylase activity for the effective production of muconate from lignin-related aromatic compounds Sonoki, Tomonori; Morooka, Miyuki; Sakamoto, Kimitoshi Journal of Biotechnology, Vol. 192 https://doi.org/10.1016/j.jbiotec.2014.10.027	journal	December 2014
Combining Reclaimed PET with Bio-based Monomers Enables Plastics Upcycling Rorrer, Nicholas A.; Nicholson, Scott; Carpenter, Alberta Joule, Vol. 3, Issue 4 https://doi.org/10.1016/j.joule.2019.01.018	journal	April 2019
Innovative Chemicals and Materials from Bacterial Aromatic Catabolic Pathways Johnson, Christopher W.; Salvachúa, Davinia; Rorrer, Nicholas A. Joule, Vol. 3, Issue 6 https://doi.org/10.1016/j.joule.2019.05.011	journal	June 2019
Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity Johnson, Christopher W.; Salvachúa, Davinia; Khanna, Payal Metabolic Engineering Communications, Vol. 3 https://doi.org/10.1016/j.meteno.2016.04.002	journal	December 2016
A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: Application for DNA fragment transfer between chromosomes and plasmid transformation Choi, Kyoung-Hee; Kumar, Ayush; Schweizer, Herbert P. Journal of Microbiological Methods, Vol. 64, Issue 3 https://doi.org/10.1016/j.mimet.2005.06.001	journal	March 2006
Robustness of Pseudomonas putida KT2440 as a host for ethanol biosynthesis Nikel, Pablo I.; de Lorenzo, Víctor New Biotechnology, Vol. 31, Issue 6 https://doi.org/10.1016/j.nbt.2014.02.006	journal	December 2014
Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin Johnson, Christopher W.; Beckham, Gregg T. Metabolic Engineering, Vol. 28 https://doi.org/10.1016/j.ymben.2015.01.005	journal	March 2015
From lignin to nylon: Cascaded chemical and biochemical conversion using metabolically engineered Pseudomonas putida Kohlstedt, Michael; Starck, Sören; Barton, Nadja Metabolic Engineering, Vol. 47 https://doi.org/10.1016/j.ymben.2018.03.003	journal	May 2018
Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism Nikel, Pablo I.; de Lorenzo, Víctor Metabolic Engineering, Vol. 50 https://doi.org/10.1016/j.ymben.2018.05.005	journal	November 2018
Refactoring the upper sugar metabolism of Pseudomonas putida for co-utilization of cellobiose, xylose, and glucose Dvořák, Pavel; de Lorenzo, Víctor Metabolic Engineering, Vol. 48 https://doi.org/10.1016/j.ymben.2018.05.019	journal	July 2018
Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440 Bentley, Gayle J.; Narayanan, Niju; Jha, Ramesh K. Metabolic Engineering, Vol. 59 https://doi.org/10.1016/j.ymben.2020.01.001	journal	May 2020
Metabolism of syringyl lignin-derived compounds in Pseudomonas putida enables convergent production of 2-pyrone-4,6-dicarboxylic acid Notonier, Sandra; Werner, Allison Z.; Kuatsjah, Eugene Metabolic Engineering, Vol. 65 https://doi.org/10.1016/j.ymben.2021.02.005	journal	May 2021
Biological upgrading of pyrolysis-derived wastewater: Engineering Pseudomonas putida for alkylphenol, furfural, and acetone catabolism and (methyl)muconic acid production Henson, William R.; Meyers, Alex W.; Jayakody, Lahiru N. Metabolic Engineering, Vol. 68 https://doi.org/10.1016/j.ymben.2021.08.007	journal	November 2021
Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate Kuatsjah, Eugene; Johnson, Christopher W.; Salvachúa, Davinia Metabolic Engineering, Vol. 70 https://doi.org/10.1016/j.ymben.2021.12.010	journal	March 2022
Biobased PET from lignin using an engineered cis, cis-muconate-producing Pseudomonas putida strain with superior robustness, energy and redox properties Kohlstedt, Michael; Weimer, Anna; Weiland, Fabia Metabolic Engineering, Vol. 72 https://doi.org/10.1016/j.ymben.2022.05.001	journal	July 2022
Improving growth of Cupriavidus necator H16 on formate using adaptive laboratory evolution-informed engineering Calvey, Christopher H.; Sànchez i. Nogué, Violeta; White, Aleena M. Metabolic Engineering, Vol. 75 https://doi.org/10.1016/j.ymben.2022.10.016	journal	January 2023
Initiation of fatty acid biosynthesis in Pseudomonas putida KT2440 McNaught, Kevin J.; Kuatsjah, Eugene; Zahn, Michael Metabolic Engineering, Vol. 76 https://doi.org/10.1016/j.ymben.2023.02.006	journal	March 2023
Muconic Acid Production Using Engineered Pseudomonas putida KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin Almqvist, Henrik; Veras, Henrique; Li, Kena ACS Sustainable Chemistry & Engineering, Vol. 9, Issue 24 https://doi.org/10.1021/acssuschemeng.1c00933	journal	June 2021
Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida Dvořák, Pavel; Bayer, Edward A.; de Lorenzo, Víctor ACS Synthetic Biology, Vol. 9, Issue 10 https://doi.org/10.1021/acssynbio.0c00276	journal	September 2020
Less Is More: Toward a Genome-Reduced Bacillus Cell Factory for “Difficult Proteins” Aguilar Suárez, Rocío; Stülke, Jörg; van Dijl, Jan Maarten ACS Synthetic Biology, Vol. 8, Issue 1 https://doi.org/10.1021/acssynbio.8b00342	journal	December 2018
Streamlined Pseudomonas taiwanensis VLB120 Chassis Strains with Improved Bioprocess Features Wynands, Benedikt; Otto, Maike; Runge, Nadine ACS Synthetic Biology, Vol. 8, Issue 9 https://doi.org/10.1021/acssynbio.9b00108	journal	August 2019
Environmentally compatible synthesis of adipic acid from D-glucose Draths, Karen M.; Frost, John W. Journal of the American Chemical Society, Vol. 116, Issue 1, p. 399-400 https://doi.org/10.1021/ja00080a057	journal	January 1994
Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system Yu, Byung Jo; Sung, Bong Hyun; Koob, Michael D. Nature Biotechnology, Vol. 20, Issue 10 https://doi.org/10.1038/nbt740	journal	September 2002
Biotechnological domestication of pseudomonads using synthetic biology Nikel, Pablo I.; Martínez-García, Esteban; de Lorenzo, Víctor Nature Reviews Microbiology, Vol. 12, Issue 5 https://doi.org/10.1038/nrmicro3253	journal	April 2014
Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering Ling, Chen; Peabody, George L.; Salvachúa, Davinia Nature Communications, Vol. 13, Issue 1 https://doi.org/10.1038/s41467-022-32296-y	journal	August 2022
Bio-based polymers with performance-advantaged properties Cywar, Robin M.; Rorrer, Nicholas A.; Hoyt, Caroline B. Nature Reviews Materials, Vol. 7, Issue 2 https://doi.org/10.1038/s41578-021-00363-3	journal	September 2021
Assembly of long, error-prone reads using repeat graphs Kolmogorov, Mikhail; Yuan, Jeffrey; Lin, Yu Nature Biotechnology, Vol. 37, Issue 5 https://doi.org/10.1038/s41587-019-0072-8	journal	April 2019
Time- and memory-efficient genome assembly with Raven Vaser, Robert; Šikić, Mile Nature Computational Science, Vol. 1, Issue 5 https://doi.org/10.1038/s43588-021-00073-4	journal	May 2021
Adipic acid production from lignin Vardon, Derek R.; Franden, Mary Ann; Johnson, Christopher W. Energy & Environmental Science, Vol. 8, Issue 2 https://doi.org/10.1039/C4EE03230F	journal	January 2015
cis,cis-Muconic acid: separation and catalysis to bio-adipic acid for nylon-6,6 polymerization Vardon, Derek R.; Rorrer, Nicholas A.; Salvachúa, Davinia Green Chemistry, Vol. 18, Issue 11 https://doi.org/10.1039/C5GC02844B	journal	January 2016
Bioprivileged molecules: creating value from biomass Shanks, Brent H.; Keeling, Peter L. Green Chemistry, Vol. 19, Issue 14 https://doi.org/10.1039/C7GC00296C	journal	January 2017
Thermochemical wastewater valorization via enhanced microbial toxicity tolerance Jayakody, Lahiru N.; Johnson, Christopher W.; Whitham, Jason M. Energy & Environmental Science, Vol. 11, Issue 6 https://doi.org/10.1039/C8EE00460A	journal	January 2018
Bioprocess development for muconic acid production from aromatic compounds and lignin Salvachúa, Davinia; Johnson, Christopher W.; Singer, Christine A. Green Chemistry, Vol. 20, Issue 21 https://doi.org/10.1039/C8GC02519C	journal	January 2018
Schizosaccharomyces pombe minimum genome factory Giga‐Hama, Yuko; Tohda, Hideki; Takegawa, Kaoru Biotechnology and Applied Biochemistry, Vol. 46, Issue 3 https://doi.org/10.1042/BA20060106	journal	March 2007
Escherichia coli minimum genome factory Mizoguchi, Hiroshi; Mori, Hideo; Fujio, Tatsuro Biotechnology and Applied Biochemistry, Vol. 46, Issue 3 https://doi.org/10.1042/BA20060107	journal	March 2007
Bacillus minimum genome factory: effective utilization of microbial genome information Ara, Katsutoshi; Ozaki, Katsuya; Nakamura, Kouji Biotechnology and Applied Biochemistry, Vol. 46, Issue 3 https://doi.org/10.1042/BA20060111	journal	March 2007
Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440 Jimenez, Jose Ignacio; Minambres, Baltasar; Garcia, Jose Luis Environmental Microbiology, Vol. 4, Issue 12, p. 824-841 https://doi.org/10.1046/j.1462-2920.2002.00370.x	journal	December 2002
Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism Komatsu, M.; Uchiyama, T.; Omura, S. Proceedings of the National Academy of Sciences, Vol. 107, Issue 6 https://doi.org/10.1073/pnas.0914833107	journal	January 2010
Lignin valorization through integrated biological funneling and chemical catalysis Linger, J. G.; Vardon, D. R.; Guarnieri, M. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018 https://doi.org/10.1073/pnas.1410657111	journal	August 2014
Pseudomonas putida KT2440 Strain Metabolizes Glucose through a Cycle Formed by Enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and Pentose Phosphate Pathways Nikel, Pablo I.; Chavarría, Max; Fuhrer, Tobias Journal of Biological Chemistry, Vol. 290, Issue 43 https://doi.org/10.1074/jbc.M115.687749	journal	September 2015
fastp: an ultra-fast all-in-one FASTQ preprocessor Chen, Shifu; Zhou, Yanqing; Chen, Yaru Bioinformatics, Vol. 34, Issue 17 https://doi.org/10.1093/bioinformatics/bty560	journal	September 2018
Correlation between genome reduction and bacterial growth Kurokawa, Masaomi; Seno, Shigeto; Matsuda, Hideo DNA Research, Vol. 23, Issue 6 https://doi.org/10.1093/dnares/dsw035	journal	July 2016
Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering Juhas, M.; Reuss, D. R.; Zhu, B. Microbiology, Vol. 160, Issue Pt_11 https://doi.org/10.1099/mic.0.079376-0	journal	August 2014
Engineering a Reduced Escherichia coli Genome Kolisnychenko, V. Genome Research, Vol. 12, Issue 4 https://doi.org/10.1101/gr.217202	journal	April 2002
The metabolic cost of flagellar motion in P seudomonas putida KT2440 : Cost vs. benefit of Martínez-García, Esteban; Nikel, Pablo I.; Chavarría, Max Environmental Microbiology, Vol. 16, Issue 1 https://doi.org/10.1111/1462-2920.12309	journal	November 2013
Biotransformation of d ‐xylose to d ‐xylonate coupled to medium‐chain‐length polyhydroxyalkanoate production in cellobiose‐grown Pseudomonas putida EM42 Dvořák, Pavel; Kováč, Jozef; Lorenzo, Víctor Microbial Biotechnology, Vol. 13, Issue 4 https://doi.org/10.1111/1751-7915.13574	journal	May 2020
Screening of a genome‐reduced Corynebacterium glutamicum strain library for improved heterologous cutinase secretion Hemmerich, Johannes; Labib, Mohamed; Steffens, Carmen Microbial Biotechnology, Vol. 13, Issue 6 https://doi.org/10.1111/1751-7915.13660	journal	September 2020
Cell size and nucleoid organization of engineered Escherichia coli cells with a reduced genome: Engineered E. coli with a reduced genome Hashimoto, Masayuki; Ichimura, Toshiharu; Mizoguchi, Hiroshi Molecular Microbiology, Vol. 55, Issue 1 https://doi.org/10.1111/j.1365-2958.2004.04386.x	journal	November 2004
Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440: Tools for editing Gram-negative genomes Martínez-García, Esteban; de Lorenzo, Víctor Environmental Microbiology, Vol. 13, Issue 10 https://doi.org/10.1111/j.1462-2920.2011.02538.x	journal	August 2011
Integration of chemotaxis, transport and catabolism inPseudomonas putidaand identification of the aromatic acid chemoreceptor PcaY Luu, Rita A.; Kootstra, Joshua D.; Nesteryuk, Vasyl Molecular Microbiology, Vol. 96, Issue 1 https://doi.org/10.1111/mmi.12929	journal	February 2015
beta -Ketoacyl-Acyl Carrier Protein Synthase III (FabH) Is a Determining Factor in Branched-Chain Fatty Acid Biosynthesis Choi, K. -H.; Heath, R. J.; Rock, C. O. Journal of Bacteriology, Vol. 182, Issue 2 https://doi.org/10.1128/JB.182.2.365-370.2000	journal	January 2000
Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production Lee, Jun; Sung, Bong; Kim, Mi Microbial Cell Factories, Vol. 8, Issue 1 https://doi.org/10.1186/1475-2859-8-2	journal	January 2009
Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression Martínez-García, Esteban; Nikel, Pablo I.; Aparicio, Tomás Microbial Cell Factories, Vol. 13, Issue 1 https://doi.org/10.1186/s12934-014-0159-3	journal	November 2014
Genome reduction boosts heterologous gene expression in Pseudomonas putida Lieder, Sarah; Nikel, Pablo I.; de Lorenzo, Víctor Microbial Cell Factories, Vol. 14, Issue 1 https://doi.org/10.1186/s12934-015-0207-7	journal	January 2015
Deletion of genomic islands in the Pseudomonas putida KT2440 genome can create an optimal chassis for synthetic biology applications Liang, Peixin; Zhang, Yiting; Xu, Bo Microbial Cell Factories, Vol. 19, Issue 1 https://doi.org/10.1186/s12934-020-01329-w	journal	March 2020
Engineering of a genome-reduced strain Bacillus amyloliquefaciens for enhancing surfactin production Zhang, Fang; Huo, Kaiyue; Song, Xingyi Microbial Cell Factories, Vol. 19, Issue 1 https://doi.org/10.1186/s12934-020-01485-z	journal	December 2020
Trycycler: consensus long-read assemblies for bacterial genomes Wick, Ryan R.; Judd, Louise M.; Cerdeira, Louise T. Genome Biology, Vol. 22, Issue 1 https://doi.org/10.1186/s13059-021-02483-z	journal	September 2021
Identification of the two-component guaiacol demethylase system from Rhodococcus rhodochrous and expression in Pseudomonas putida EM42 for guaiacol assimilation García-Hidalgo, Javier; Ravi, Krithika; Kuré, Lise-Lotte AMB Express, Vol. 9, Issue 1 https://doi.org/10.1186/s13568-019-0759-8	journal	March 2019
Benchmarking of long-read assemblers for prokaryote whole genome sequencing Wick, Ryan R.; Holt, Kathryn E. F1000Research, Vol. 8 https://doi.org/10.12688/f1000research.21782.1	journal	December 2019
Polypolish: Short-read polishing of long-read bacterial genome assemblies Wick, Ryan R.; Holt, Kathryn E. PLOS Computational Biology, Vol. 18, Issue 1 https://doi.org/10.1371/journal.pcbi.1009802	journal	January 2022
Bioproduction of propionic acid using levulinic acid by engineered Pseudomonas putida Tiwari, Rameshwar; Sathesh-Prabu, Chandran; Lee, Sung Kuk Frontiers in Bioengineering and Biotechnology, Vol. 10 https://doi.org/10.3389/fbioe.2022.939248	journal	August 2022
Microbial Valorization of Lignin to Bioplastic by Genome-Reduced Pseudomonas putida Zong, Qiu-Jin; Xu, Tao; Liu, He Frontiers in Microbiology, Vol. 13 https://doi.org/10.3389/fmicb.2022.923664	journal	May 2022
Experimental Challenges for Reduced Genomes: The Cell Model Escherichia coli Kurokawa, Masaomi; Ying, Bei-Wen Microorganisms, Vol. 8, Issue 1 https://doi.org/10.3390/microorganisms8010003	journal	December 2019

Similar Records

Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440

Journal Article · Fri Jan 10 00:00:00 UTC 2020 · Metabolic Engineering · OSTI ID:1596244

Bentley, Gayle; Narayanan, Niju; Jha, Ramesh K.; +16 more

Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin

Journal Article · Fri Aug 30 00:00:00 UTC 2019 · Microbial Biotechnology (Online) · OSTI ID:1559676

Salvachúa, Davinia; Rydzak, Thomas; Auwae, Raquel; +13 more

Related Subjects

09 BIOMASS FUELS
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Pseudomonas putida EM42
Pseudomonas putida KT2440
aromatic catabolism
genome reduction
muconic acid

Title: Comparison of wild-type KT2440 and genome-reduced EM42 Pseudomonas putida strains for muconate production from aromatic compounds and glucose

Citation Formats

References (73)

Similar Records

Related Subjects