Lignin conversion to β-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development

Werner, Allison Z.; Cordell, William T.; Lahive, Ciaran W.; Klein, Bruno C.; Singer, Christine A.; Tan, Eric C. D.; Ingraham, Morgan A.; Ramirez, Kelsey J.; Kim, Dong Hyun; Pedersen, Jacob Nedergaard; Johnson, Christopher W.; Pfleger, Brian F.; Beckham, Gregg T.; Salvachúa, Davinia

doi:10.1126/sciadv.adj0053

Title: Lignin conversion to β-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development

Journal Article · Wed Sep 06 00:00:00 UTC 2023 · Science Advances

DOI: https://doi.org/10.1126/sciadv.adj0053 · OSTI ID:2004925

^[1];

^[2];

^[1];

^[3];

^[1];

^[3];

^[1];

^[1]; Pedersen, Jacob Nedergaard ^[1];

^[1];

^[4];

^[1];

^[1]

National Renewable Energy Laboratory (NREL), Golden, CO (United States). Renewable Resources and Enabling Science Center
National Renewable Energy Laboratory (NREL), Golden, CO (United States). Renewable Resources and Enabling Science Center; Univ. of Wisconsin, Madison, WI (United States)
National Renewable Energy Laboratory (NREL), Golden, CO (United States). Catalytic Carbon Transformation and Scale-Up Center
Univ. of Wisconsin, Madison, WI (United States)

Bioconversion of a heterogeneous mixture of lignin-related aromatic compounds (LRCs) to a single product via microbial biocatalysts is a promising approach to valorize lignin. Here, Pseudomonas putida KT2440 was engineered to convert mixed p-coumaroyl– and coniferyl-type LRCs to β-ketoadipic acid, a precursor for performance-advantaged polymers. Expression of enzymes mediating aromatic O-demethylation, hydroxylation, and ring-opening steps was tuned, and a global regulator was deleted. β-ketoadipate titers of 44.5 and 25 grams per liter and productivities of 1.15 and 0.66 grams per liter per hour were achieved from model LRCs and corn stover-derived LRCs, respectively, the latter representing an overall yield of 0.10 grams per gram corn stover-derived lignin. Technoeconomic analysis of the bioprocess and downstream processing predicted a β-ketoadipate minimum selling price of $$\$$2.01$ per kilogram, which is cost competitive with fossil carbon-derived adipic acid ($$\$$1.10$ to 1.80 per kilogram). Overall, this work achieved bioproduction metrics with economic relevance for conversion of lignin-derived streams into a performance-advantaged bioproduct.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Office of Workforce Development for Teachers & Scientists (WDTS)

Grant/Contract Number:: AC36-08GO28308; SC0014664

OSTI ID:: 2004925

Report Number(s):: NREL/JA--2A00-86909; MainId:87684; UUID:1ab3e9dc-b50c-4ccd-829e-fc2ca897a04f; MainAdminID:70681

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 36 Vol. 9; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

References (77)

Advanced Biotechnology: Metabolically Engineered Cells for the Bio-Based Production of Chemicals and Fuels, Materials, and Health-Care Products Becker, Judith; Wittmann, Christoph Angewandte Chemie International Edition, Vol. 54, Issue 11 https://doi.org/10.1002/anie.201409033	journal	February 2015
Paving the Way for Lignin Valorisation: Recent Advances in Bioengineering, Biorefining and Catalysis Rinaldi, Roberto; Jastrzebski, Robin; Clough, Matthew T. Angewandte Chemie International Edition, Vol. 55, Issue 29 https://doi.org/10.1002/anie.201510351	journal	June 2016
Temperature-Directed Biocatalysis for the Sustainable Production of Aromatic Aldehydes or Alcohols Ni, Jun; Gao, Yan-Yan; Tao, Fei Angewandte Chemie International Edition, Vol. 57, Issue 5 https://doi.org/10.1002/anie.201710793	journal	December 2017
Life-cycle analysis of integrated biorefineries with co-production of biofuels and bio-based chemicals: co-product handling methods and implications: Co-product handling methods and implications of life-cycle analysis of biofuel and bio-chemical co-producing biorefineries Cai, Hao; Han, Jeongwoo; Wang, Michael Biofuels, Bioproducts and Biorefining, Vol. 12, Issue 5 https://doi.org/10.1002/bbb.1893	journal	June 2018
The costs of sugar production from different feedstocks and processing technologies Cheng, Ming‐Hsun; Huang, Haibo; Dien, Bruce S. Biofuels, Bioproducts and Biorefining, Vol. 13, Issue 3 https://doi.org/10.1002/bbb.1976	journal	December 2018
Production of Substituted Styrene Bioproducts from Lignin and Lignocellulose Using Engineered Pseudomonas putida KT2440 Williamson, James J.; Bahrin, Nurfariza; Hardiman, Elizabeth M. Biotechnology Journal, Vol. 15, Issue 7 https://doi.org/10.1002/biot.201900571	journal	June 2020
Assessing the Viability of Recovery of Hydroxycinnamic Acids from Lignocellulosic Biorefinery Alkaline Pretreatment Waste Streams Karlen, Steven D.; Fasahati, Peyman; Mazaheri, Mona ChemSusChem, Vol. 13, Issue 8 https://doi.org/10.1002/cssc.201903345	journal	March 2020
Industrial biotechnology of Pseudomonas putida and related species Poblete-Castro, Ignacio; Becker, Judith; Dohnt, Katrin Applied Microbiology and Biotechnology, Vol. 93, Issue 6 https://doi.org/10.1007/s00253-012-3928-0	journal	February 2012
Genetic tools for reliable gene expression and recombineering in Pseudomonas putida Cook, Taylor B.; Rand, Jacqueline M.; Nurani, Wasti Journal of Industrial Microbiology & Biotechnology, Vol. 45, Issue 7 https://doi.org/10.1007/s10295-017-2001-5	journal	January 2018
Hydroxycinnamates in lignification Ralph, John Phytochemistry Reviews, Vol. 9, Issue 1, p. 65-83 https://doi.org/10.1007/s11101-009-9141-9	journal	August 2009
The ecoinvent database version 3 (part I): overview and methodology Wernet, Gregor; Bauer, Christian; Steubing, Bernhard The International Journal of Life Cycle Assessment, Vol. 21, Issue 9 https://doi.org/10.1007/s11367-016-1087-8	journal	April 2016
Lignin valorization through efficient microbial production of β-ketoadipate from industrial black liquor Suzuki, Yuzo; Otsuka, Yuichiro; Araki, Takuma Bioresource Technology, Vol. 337 https://doi.org/10.1016/j.biortech.2021.125489	journal	October 2021
Efficient vanillin biosynthesis by recombinant lignin-degrading bacterium Arthrobacter sp. C2 and its environmental profile via life cycle assessment Zhao, Xinyue; Zhang, Yuting; Jiang, Hanyi Bioresource Technology, Vol. 347 https://doi.org/10.1016/j.biortech.2021.126434	journal	March 2022
Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains Gómez-Álvarez, Helena; Iturbe, Pablo; Rivero-Buceta, Virginia Bioresource Technology, Vol. 346 https://doi.org/10.1016/j.biortech.2021.126638	journal	February 2022
Integrated process development for grass biomass utilization through enzymatic saccharification and upgrading hydroxycinnamic acids via microbial funneling Suzuki, Yuzo; Okamura-Abe, Yuriko; Otsuka, Yuichiro Bioresource Technology, Vol. 363 https://doi.org/10.1016/j.biortech.2022.127836	journal	November 2022
Funneling lignin-derived compounds into polyhydroxyalkanoate by Halomonas sp. Y3 Tang, Hao; Wang, Ming-Jun; Gan, Xiao-Feng Bioresource Technology, Vol. 362 https://doi.org/10.1016/j.biortech.2022.127837	journal	October 2022
Biological valorization of low molecular weight lignin Abdelaziz, Omar Y.; Brink, Daniel P.; Prothmann, Jens Biotechnology Advances, Vol. 34, Issue 8 https://doi.org/10.1016/j.biotechadv.2016.10.001	journal	December 2016
A navigation guide of synthetic biology tools for Pseudomonas putida Martin-Pascual, Maria; Batianis, Christos; Bruinsma, Lyon Biotechnology Advances, Vol. 49 https://doi.org/10.1016/j.biotechadv.2021.107732	journal	July 2021
From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions Nikel, Pablo I.; Chavarría, Max; Danchin, Antoine Current Opinion in Chemical Biology, Vol. 34 https://doi.org/10.1016/j.cbpa.2016.05.011	journal	October 2016
Discovery, characterization, and metabolic engineering of Rieske non-heme iron monooxygenases for guaiacol O-demethylation Bleem, Alissa; Kuatsjah, Eugene; Presley, Gerald N. Chem Catalysis, Vol. 2, Issue 8 https://doi.org/10.1016/j.checat.2022.04.019	journal	August 2022
Beta-ketoadipic acid and muconolactone production from a lignin-related aromatic compound through the protocatechuate 3,4-metabolic pathway Okamura-Abe, Yuriko; Abe, Tomokuni; Nishimura, Kei Journal of Bioscience and Bioengineering, Vol. 121, Issue 6 https://doi.org/10.1016/j.jbiosc.2015.11.007	journal	June 2016
Enhancement of protocatechuate decarboxylase activity for the effective production of muconate from lignin-related aromatic compounds Sonoki, Tomonori; Morooka, Miyuki; Sakamoto, Kimitoshi Journal of Biotechnology, Vol. 192 https://doi.org/10.1016/j.jbiotec.2014.10.027	journal	December 2014
Lignin as a potential source of high-added value compounds: A review Zevallos Torres, Luis Alberto; Lorenci Woiciechowski, Adenise; de Andrade Tanobe, Valcineide Oliveira Journal of Cleaner Production, Vol. 263 https://doi.org/10.1016/j.jclepro.2020.121499	journal	August 2020
Innovative Chemicals and Materials from Bacterial Aromatic Catabolic Pathways Johnson, Christopher W.; Salvachúa, Davinia; Rorrer, Nicholas A. Joule, Vol. 3, Issue 6 https://doi.org/10.1016/j.joule.2019.05.011	journal	June 2019
Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance Mohamed, Elsayed T.; Werner, Allison Z.; Salvachúa, Davinia Metabolic Engineering Communications, Vol. 11 https://doi.org/10.1016/j.mec.2020.e00143	journal	December 2020
Dynamic and single cell characterization of a CRISPR-interference toolset in Pseudomonas putida KT2440 for β-ketoadipate production from p-coumarate Fenster, Jacob A.; Werner, Allison Z.; Tay, Jian Wei Metabolic Engineering Communications, Vol. 15 https://doi.org/10.1016/j.mec.2022.e00204	journal	December 2022
Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate in Pseudomonas putida KT2440 Johnson, Christopher W.; Abraham, Paul E.; Linger, Jeffrey G. Metabolic Engineering Communications, Vol. 5 https://doi.org/10.1016/j.meteno.2017.05.002	journal	December 2017
A protocatechuate biosensor for Pseudomonas putida KT2440 via promoter and protein evolution Jha, Ramesh K.; Bingen, Jeremy M.; Johnson, Christopher W. Metabolic Engineering Communications, Vol. 6 https://doi.org/10.1016/j.meteno.2018.03.001	journal	June 2018
Production of β-ketoadipic acid from glucose in Pseudomonas putida KT2440 for use in performance-advantaged nylons Rorrer, Nicholas A.; Notonier, Sandra F.; Knott, Brandon C. Cell Reports Physical Science, Vol. 3, Issue 4 https://doi.org/10.1016/j.xcrp.2022.100840	journal	April 2022
Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin Johnson, Christopher W.; Beckham, Gregg T. Metabolic Engineering, Vol. 28 https://doi.org/10.1016/j.ymben.2015.01.005	journal	March 2015
From lignin to nylon: Cascaded chemical and biochemical conversion using metabolically engineered Pseudomonas putida Kohlstedt, Michael; Starck, Sören; Barton, Nadja Metabolic Engineering, Vol. 47 https://doi.org/10.1016/j.ymben.2018.03.003	journal	May 2018
Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism Nikel, Pablo I.; de Lorenzo, Víctor Metabolic Engineering, Vol. 50 https://doi.org/10.1016/j.ymben.2018.05.005	journal	November 2018
Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440 Bentley, Gayle J.; Narayanan, Niju; Jha, Ramesh K. Metabolic Engineering, Vol. 59 https://doi.org/10.1016/j.ymben.2020.01.001	journal	May 2020
Characterization of aromatic acid/proton symporters in Pseudomonas putida KT2440 toward efficient microbial conversion of lignin-related aromatics Wada, Ayumu; Prates, Érica T.; Hirano, Ryo Metabolic Engineering, Vol. 64 https://doi.org/10.1016/j.ymben.2021.01.013	journal	March 2021
Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound Presley, Gerald N.; Werner, Allison Z.; Katahira, Rui Metabolic Engineering, Vol. 65 https://doi.org/10.1016/j.ymben.2021.02.003	journal	May 2021
Metabolism of syringyl lignin-derived compounds in Pseudomonas putida enables convergent production of 2-pyrone-4,6-dicarboxylic acid Notonier, Sandra; Werner, Allison Z.; Kuatsjah, Eugene Metabolic Engineering, Vol. 65 https://doi.org/10.1016/j.ymben.2021.02.005	journal	May 2021
Engineering Pseudomonas putida for efficient aromatic conversion to bioproduct using high throughput screening in a bioreactor Eng, Thomas; Banerjee, Deepanwita; Lau, Andrew K. Metabolic Engineering, Vol. 66 https://doi.org/10.1016/j.ymben.2021.04.015	journal	July 2021
Guiding stars to the field of dreams: Metabolically engineered pathways and microbial platforms for a sustainable lignin-based industry Weiland, Fabia; Kohlstedt, Michael; Wittmann, Christoph Metabolic Engineering, Vol. 71 https://doi.org/10.1016/j.ymben.2021.11.011	journal	May 2022
The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future Tiso, Till; Winter, Benedikt; Wei, Ren Metabolic Engineering, Vol. 71 https://doi.org/10.1016/j.ymben.2021.12.006	journal	May 2022
Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate Kuatsjah, Eugene; Johnson, Christopher W.; Salvachúa, Davinia Metabolic Engineering, Vol. 70 https://doi.org/10.1016/j.ymben.2021.12.010	journal	March 2022
RB-TnSeq identifies genetic targets for improved tolerance of Pseudomonas putida towards compounds relevant to lignin conversion Borchert, Andrew J.; Bleem, Alissa; Beckham, Gregg T. Metabolic Engineering, Vol. 77 https://doi.org/10.1016/j.ymben.2023.04.007	journal	May 2023
Bright Side of Lignin Depolymerization: Toward New Platform Chemicals Sun, Zhuohua; Fridrich, Bálint; de Santi, Alessandra Chemical Reviews, Vol. 118, Issue 2 https://doi.org/10.1021/acs.chemrev.7b00588	journal	January 2018
From Lignin to Valuable Aromatic Chemicals: Lignin Depolymerization and Monomer Separation via Centrifugal Partition Chromatography Alherech, Manar; Omolabake, Surajudeen; Holland, Christopher M. ACS Central Science, Vol. 7, Issue 11 https://doi.org/10.1021/acscentsci.1c00729	journal	October 2021
Muconic Acid Production Using Engineered Pseudomonas putida KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin Almqvist, Henrik; Veras, Henrique; Li, Kena ACS Sustainable Chemistry & Engineering, Vol. 9, Issue 24 https://doi.org/10.1021/acssuschemeng.1c00933	journal	June 2021
Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion Rodriguez, Alberto; Salvachúa, Davinia; Katahira, Rui ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 9 https://doi.org/10.1021/acssuschemeng.7b01818	journal	August 2017
The Catalytic Valorization of Lignin for the Production of Renewable Chemicals Zakzeski, Joseph; Bruijnincx, Pieter C. A.; Jongerius, Anna L. Chemical Reviews, Vol. 110, Issue 6, p. 3552-3599 https://doi.org/10.1021/cr900354u	journal	June 2010
Oxidative Catalytic Fractionation of Lignocellulosic Biomass under Non-alkaline Conditions Luo, Hao; Weeda, Eric P.; Alherech, Manar Journal of the American Chemical Society, Vol. 143, Issue 37 https://doi.org/10.1021/jacs.1c08635	journal	September 2021
Alkaline Pretreatment of Corn Stover: Bench-Scale Fractionation and Stream Characterization Karp, Eric M.; Donohoe, Bryon S.; O’Brien, Marykate H. ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 6, p. 1481-1491 https://doi.org/10.1021/sc500126u	journal	May 2014
UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis White, Mark D.; Payne, Karl A. P.; Fisher, Karl Nature, Vol. 522, Issue 7557 https://doi.org/10.1038/nature14559	journal	June 2015
In Situ Product Removal as a Tool for Bioprocessing Freeman, Amihay; Woodley, John M.; Lilly, Malcolm D. Nature Biotechnology, Vol. 11, Issue 9 https://doi.org/10.1038/nbt0993-1007	journal	September 1993
Whole plant cell wall characterization using solution-state 2D NMR Mansfield, Shawn D.; Kim, Hoon; Lu, Fachuang Nature Protocols, Vol. 7, Issue 9 https://doi.org/10.1038/nprot.2012.064	journal	August 2012
Production of itaconic acid from alkali pretreated lignin by dynamic two stage bioconversion Elmore, Joshua R.; Dexter, Gara N.; Salvachúa, Davinia Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-22556-8	journal	April 2021
Oxidative cleavage of C–C bonds in lignin Subbotina, Elena; Rukkijakan, Thanya; Marquez-Medina, M. Dolores Nature Chemistry, Vol. 13, Issue 11 https://doi.org/10.1038/s41557-021-00783-2	journal	September 2021
A metabolic pathway for catabolizing levulinic acid in bacteria Rand, Jacqueline M.; Pisithkul, Tippapha; Clark, Ryan L. Nature Microbiology, Vol. 2, Issue 12 https://doi.org/10.1038/s41564-017-0028-z	journal	September 2017
Chemistry challenges to enable a sustainable bioeconomy Fitzgerald, Nichole D. Nature Reviews Chemistry, Vol. 1, Issue 10 https://doi.org/10.1038/s41570-017-0080	journal	September 2017
Bio-based polymers with performance-advantaged properties Cywar, Robin M.; Rorrer, Nicholas A.; Hoyt, Caroline B. Nature Reviews Materials, Vol. 7, Issue 2 https://doi.org/10.1038/s41578-021-00363-3	journal	September 2021
Critical enzyme reactions in aromatic catabolism for microbial lignin conversion Erickson, Erika; Bleem, Alissa; Kuatsjah, Eugene Nature Catalysis, Vol. 5, Issue 2 https://doi.org/10.1038/s41929-022-00747-w	journal	February 2022
Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T. Green Chemistry, Vol. 17, Issue 11 https://doi.org/10.1039/C5GC01165E	journal	January 2015
Chemicals from lignin: an interplay of lignocellulose fractionation, depolymerisation, and upgrading Schutyser, W.; Renders, T.; Van den Bosch, S. Chemical Society Reviews, Vol. 47, Issue 3 https://doi.org/10.1039/C7CS00566K	journal	January 2018
Life cycle assessment of adipic acid production from lignin Corona, Andrea; Biddy, Mary J.; Vardon, Derek R. Green Chemistry, Vol. 20, Issue 16 https://doi.org/10.1039/C8GC00868J	journal	January 2018
Bioprocess development for muconic acid production from aromatic compounds and lignin Salvachúa, Davinia; Johnson, Christopher W.; Singer, Christine A. Green Chemistry, Vol. 20, Issue 21 https://doi.org/10.1039/C8GC02519C	journal	January 2018
Funneling aromatic products of chemically depolymerized lignin into 2-pyrone-4-6-dicarboxylic acid with Novosphingobium aromaticivorans Perez, Jose M.; Kontur, Wayne S.; Alherech, Manar Green Chemistry, Vol. 21, Issue 6 https://doi.org/10.1039/C8GC03504K	journal	January 2019
Guidelines for performing lignin-first biorefining Abu-Omar, Mahdi M.; Barta, Katalin; Beckham, Gregg T. Energy & Environmental Science, Vol. 14, Issue 1 https://doi.org/10.1039/D0EE02870C	journal	January 2021
Recovery of low molecular weight compounds from alkaline pretreatment liquor via membrane separations Saboe, Patrick O.; Tomashek, Emily G.; Monroe, Hanna R. Green Chemistry, Vol. 24, Issue 8 https://doi.org/10.1039/D2GC00075J	journal	January 2022
Lignin valorization through integrated biological funneling and chemical catalysis Linger, J. G.; Vardon, D. R.; Guarnieri, M. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018 https://doi.org/10.1073/pnas.1410657111	journal	August 2014
Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin Salvachúa, Davinia; Rydzak, Thomas; Auwae, Raquel Microbial Biotechnology, Vol. 13, Issue 1 https://doi.org/10.1111/1751-7915.13481	journal	January 2020
Bacterial catabolism of lignin-derived aromatics: New findings in a recent decade: Update on bacterial lignin catabolism: Bacterial catabolism of lignin-derived aromatics Kamimura, Naofumi; Takahashi, Kenji; Mori, Kosuke Environmental Microbiology Reports, Vol. 9, Issue 6 https://doi.org/10.1111/1758-2229.12597	journal	November 2017
Gac/Rsm signal transduction pathway of γ-proteobacteria: from RNA recognition to regulation of social behaviour: Regulation of RsmA/CsrA binding to RNA Lapouge, Karine; Schubert, Mario; Allain, Frédéric H. -T. Molecular Microbiology, Vol. 67, Issue 2 https://doi.org/10.1111/j.1365-2958.2007.06042.x	journal	November 2007
The translational repressor Crc controls the Pseudomonas putida benzoate and alkane catabolic pathways using a multi‐tier regulation strategy Hernández-Arranz, Sofía; Moreno, Renata; Rojo, Fernando Environmental Microbiology, Vol. 15, Issue 1, p. 227-241 https://doi.org/10.1111/j.1462-2920.2012.02863.x	journal	August 2012
Carbon catabolite repression in Pseudomonas : optimizing metabolic versatility and interactions with the environment Rojo, Fernando FEMS Microbiology Reviews, Vol. 34, Issue 5 https://doi.org/10.1111/j.1574-6976.2010.00218.x	journal	September 2010
Lignin Valorization: Improving Lignin Processing in the Biorefinery Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J. Science, Vol. 344, Issue 6185, p. 1246843-1246843 https://doi.org/10.1126/science.1246843	journal	May 2014
Mixed plastics waste valorization through tandem chemical oxidation and biological funneling Sullivan, Kevin P.; Werner, Allison Z.; Ramirez, Kelsey J. Science, Vol. 378, Issue 6616 https://doi.org/10.1126/science.abo4626	journal	October 2022
Roles of Cyclic Di-GMP and the Gac System in Transcriptional Control of the Genes Coding for the Pseudomonas putida Adhesins LapA and LapF Martinez-Gil, M.; Ramos-Gonzalez, M. I.; Espinosa-Urgel, M. Journal of Bacteriology, Vol. 196, Issue 8 https://doi.org/10.1128/JB.01287-13	journal	January 2014
The β-Ketoadipate Pathway And The Biology Of Self-Identity Harwood, Caroline S.; Parales, Rebecca E. Annual Review of Microbiology, Vol. 50, Issue 1, p. 553-590 https://doi.org/10.1146/annurev.micro.50.1.553	journal	October 1996
Metabolic engineering of Rhodococcus jostii RHA1 for production of pyridine-dicarboxylic acids from lignin Spence, Edward M.; Calvo-Bado, Leonides; Mines, Paul Microbial Cell Factories, Vol. 20, Issue 1 https://doi.org/10.1186/s12934-020-01504-z	journal	January 2021
Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbon Fuels and Coproducts: 2018 Biochemical Design Case Update; Biochemical Deconstruction and Conversion of Biomass to Fuels and Products via Integrated Biorefinery Pathways Davis, Ryan E.; Grundl, Nicholas J.; Tao, Ling https://doi.org/10.2172/1483234	report	November 2018
Biosynthesis of Commodity Chemicals From Oil Palm Empty Fruit Bunch Lignin Lo, Tat-Ming; Hwang, In Young; Cho, Han-Saem Frontiers in Microbiology, Vol. 12 https://doi.org/10.3389/fmicb.2021.663642	journal	April 2021

Similar Records

Dynamic and single cell characterization of a CRISPR-interference toolset in Pseudomonas putida KT2440 for β-ketoadipate production from p-coumarate

Journal Article · Sun Aug 28 00:00:00 UTC 2022 · Metabolic Engineering Communications · OSTI ID:1885323

Fenster, Jacob A.; Werner, Allison Z.; Tay, Jian Wei; +9 more

Engineered Accumulation of Protocatechuate in Corn Biomass to Enhance Biomanufacturing

Journal Article · Tue Nov 11 00:00:00 UTC 2025 · ACS Sustainable Chemistry & Engineering · OSTI ID:3005645

Tian, Yang; Kim, Bumkyu; Pamukçu, Irem; +12 more

2.3.2.100 - Biological Lignin Valorization (BLV)

Conference · Tue Apr 18 00:00:00 UTC 2023 · OSTI ID:1971856

Beckham, Gregg T.; Salvachua, Davinia

Related Subjects

09 BIOMASS FUELS
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
bioconversion
bioproduct
lignin valorization
β-ketoadipic acid

Title: Lignin conversion to β-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development

Citation Formats

References (77)

Similar Records

Related Subjects