High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water
Abstract
The genome of the basidiomycete yeast Dioszegia hungarica strain PDD-24b-2 isolated from cloud water at the summit of puy de $$D\hat{o}me$$ (France) was sequenced using a hybrid PacBio and Illumina sequencing strategy. The obtained assembled genome of 20.98 Mb and a GC content of 57% is structured in 16 large-scale contigs ranging from 90 kb to 5.56Mb, and another 27.2 kb contig representing the complete circular mitochondrial genome. In total, 8,234 proteins were predicted from the genome sequence. The mitochondrial genome shows 16.2% cgu codon usage for arginine but has no canonical cognate tRNA to translate this codon. Detected transposable element (TE)-related sequences account for about 0.63% of the assembled genome. A dataset of 2,068 hand-picked public environmental metagenomes, representing over 20 Tbp of raw reads, was probed for D. hungarica related ITS sequences, and revealed worldwide distribution of this species, particularly in aerial habitats. Growth experiments suggested a psychrophilic phenotype and the ability to disperse by producing ballistospores. The high-quality assembled genome obtained for this D. hungarica strain will help investigate the behavior and ecological functions of this species in the environment.
- Authors:
-
[1];
[2];
[1];
[3];
[1];
[1];
[4];
[3];
[1]
- Université de Strasbourg (France)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Univ. Clermont Auvergne, Clermont-Ferrand (France)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1992470
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- G3
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 12; Journal ID: ISSN 2160-1836
- Publisher:
- Genetics Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Tremellaceae; mitochondrial genome; de novo sequencing; fungi; airborne microorganisms; transposable elements; Dioszegia hungarica strain PDD-24b-2; aeromicrobiology; fungal spore; cold environment
Citation Formats
Jarrige, Domitille, Haridas, Sajeet, Bleykasten-Grosshans, Claudine, Joly, Muriel, Nadalig, Thierry, Sancelme, Martine, Vuilleumier, Stéphane, Grigoriev, Igor V., Amato, Pierre, and Bringel, Françoise. High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water. United States: N. p., 2022.
Web. doi:10.1093/g3journal/jkac282.
Jarrige, Domitille, Haridas, Sajeet, Bleykasten-Grosshans, Claudine, Joly, Muriel, Nadalig, Thierry, Sancelme, Martine, Vuilleumier, Stéphane, Grigoriev, Igor V., Amato, Pierre, & Bringel, Françoise. High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water. United States. https://doi.org/10.1093/g3journal/jkac282
Jarrige, Domitille, Haridas, Sajeet, Bleykasten-Grosshans, Claudine, Joly, Muriel, Nadalig, Thierry, Sancelme, Martine, Vuilleumier, Stéphane, Grigoriev, Igor V., Amato, Pierre, and Bringel, Françoise. Wed .
"High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water". United States. https://doi.org/10.1093/g3journal/jkac282. https://www.osti.gov/servlets/purl/1992470.
@article{osti_1992470,
title = {High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water},
author = {Jarrige, Domitille and Haridas, Sajeet and Bleykasten-Grosshans, Claudine and Joly, Muriel and Nadalig, Thierry and Sancelme, Martine and Vuilleumier, Stéphane and Grigoriev, Igor V. and Amato, Pierre and Bringel, Françoise},
abstractNote = {The genome of the basidiomycete yeast Dioszegia hungarica strain PDD-24b-2 isolated from cloud water at the summit of puy de $D\hat{o}me$ (France) was sequenced using a hybrid PacBio and Illumina sequencing strategy. The obtained assembled genome of 20.98 Mb and a GC content of 57% is structured in 16 large-scale contigs ranging from 90 kb to 5.56Mb, and another 27.2 kb contig representing the complete circular mitochondrial genome. In total, 8,234 proteins were predicted from the genome sequence. The mitochondrial genome shows 16.2% cgu codon usage for arginine but has no canonical cognate tRNA to translate this codon. Detected transposable element (TE)-related sequences account for about 0.63% of the assembled genome. A dataset of 2,068 hand-picked public environmental metagenomes, representing over 20 Tbp of raw reads, was probed for D. hungarica related ITS sequences, and revealed worldwide distribution of this species, particularly in aerial habitats. Growth experiments suggested a psychrophilic phenotype and the ability to disperse by producing ballistospores. The high-quality assembled genome obtained for this D. hungarica strain will help investigate the behavior and ecological functions of this species in the environment.},
doi = {10.1093/g3journal/jkac282},
journal = {G3},
number = 12,
volume = 12,
place = {United States},
year = {Wed Oct 19 00:00:00 EDT 2022},
month = {Wed Oct 19 00:00:00 EDT 2022}
}
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