Transcriptional Control of hgcAB by an ArsR-Like Regulator in Pseudodesulfovibrio mercurii ND132

Gionfriddo, Caitlin M.; Soren, Ally Bullock; Wymore, Ann M.; Hartnett, D. Sean; Podar, Mircea; Parks, Jerry M.; Elias, Dwayne A.; Gilmour, Cynthia C.

doi:10.1128/aem.01768-22

Title: Transcriptional Control of hgcAB by an ArsR-Like Regulator in Pseudodesulfovibrio mercurii ND132

Journal Article · Thu Mar 23 00:00:00 UTC 2023 · Applied and Environmental Microbiology

DOI: https://doi.org/10.1128/aem.01768-22 · OSTI ID:1972577

^[1]; Soren, Ally Bullock ^[1]; Wymore, Ann M. ^[2]; Hartnett, D. Sean ^[1];

^[2];

^[2]; Elias, Dwayne A. ^[3];

^[1]

Smithsonian Environmental Research Center, Edgewater, MD (United States)
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Elias Consulting, LLC, Knoxville, TN (United States)

The hgcAB gene pair encodes mercury (Hg) methylation capability in a diverse group of microorganisms, but its evolution and transcriptional regulation remain unknown. Working from the possibility that the evolutionary function of HgcAB may not be Hg methylation, we test a possible link to arsenic resistance. Using model Hg methylator Pseudodesulfovibrio mercurii ND132, we evaluated transcriptional control of hgcAB by a putative ArsR encoded upstream and cotranscribed with hgcAB. This regulator shares homology with ArsR repressors of arsenic resistance and S-adenosylhomocysteine (SAH)- responsive regulators of methionine biosynthesis but is distinct from other ArsR/SahR proteins in P. mercurii. Using quantitative PCR (qPCR) and RNA sequencing (RNA-seq) transcriptome analyses, we confirmed this ArsR regulates hgcAB transcription and is responsive to arsenic and SAH. Additionally, RNA-seq indicated a possible link between hgcAB activity and arsenic transformations, with significant upregulation of other ArsRregulated arsenic resistance operons alongside hgcAB. Interestingly, wild-type ND132 was less sensitive to As(V) (but not As(III)) than an hgcAB knockout strain, supporting the idea that hgcAB may be linked to arsenic resistance. Arsenic significantly impacted rates of Hg methylation by ND132; however, responses varied with culture conditions. Differences in growth and metabolic activity did not account for arsenic impacts on methylation. While arsenic significantly increased hgcAB expression, hgcAB gene and transcript abundance was not a good predictor of Hg methylation rates. Taken together, these results support the idea that Hg and As cycling are linked in P. mercurii ND132. Our results may hold clues to the evolution of hgcAB and the controls on Hg methylation in nature. This work reveals a link between microbial mercury methylation and arsenic resistance and may hold clues to the evolution of mercury methylation genes (hgcAB). Microbes with hgcAB produce methylmercury, a strong neurotoxin that readily accumulates in the food web. This study addresses a critical gap in our understanding about the environmental factors that control hgcAB expression. We show that hgcAB expression is controlled by an ArsR-like regulator responsive to both arsenic and S-adenosylhomocysteine in our model organism, Pseudodesulfovibrio mercurii ND132. Exposure to arsenic also significantly impacted Pseudodesulfovibrio mercurii ND132 mercury methylation rates. However, expression of hgcAB was not always a good predictor of Hg methylation rates, highlighting the roles of Hg bioavailability and other biochemical mechanisms in methylmercury production. This study improves our understanding of the controls on hgcAB expression, which is needed to better predict environmental methylmercury production

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1972577

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 4 Vol. 89; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Zhang, Lijie; Kang-Yun, Christina S.; Lu, Xia; +5 more

Contrary effects of phytoplankton Chlorella vulgaris and its exudates on mercury methylation by iron- and sulfate-reducing bacteria

Journal Article · Fri Apr 01 00:00:00 UTC 2022 · Journal of Hazardous Materials · OSTI ID:1866695

Yin, Xixiang; Wang, Lihong; Liang, Xujun; +3 more

Pseudodesulfovibrio mercurii sp. nov., a mercury-methylating bacterium isolated from sediment

Journal Article · Thu Feb 11 00:00:00 UTC 2021 · International Journal of Systematic and Evolutionary Microbiology · OSTI ID:1767872

Gilmour, Cynthia C.; Soren, Ally Bullock; Gionfriddo, Caitlin M.; +6 more

Related Subjects

RNA-seq
59 BASIC BIOLOGICAL SCIENCES
ArsR
ars operon
arsenic
arsenic resistance
hgcAB
mercury
mercury biogeochemistry
methylmercury

RNA sequencing dataset from Pseudodesulfovibrio mercurii ND132 batch cultures Gionfriddo, Caitlin; Soren, Ally; Wymore, Ann M. https://doi.org/10.25573/serc.21561189 The current record is supplemented by this dataset	dataset	January 2023
RNA sequencing dataset from Pseudodesulfovibrio mercurii ND132 washed-cell cultures Gionfriddo, Caitlin; Soren, Ally; Wymore, Ann M. https://doi.org/10.25573/serc.21561387 The current record is supplemented by this dataset	dataset	January 2023
RNA sequencing dataset from Pseudodesulfovibrio mercurii ND132 washed-cell cultures Gionfriddo, Caitlin; Soren, Ally; Wymore, Ann M. https://doi.org/10.25573/serc.21561387.v1 The current record is supplemented by this dataset	dataset	January 2023

Title: Transcriptional Control of hgcAB by an ArsR-Like Regulator in Pseudodesulfovibrio mercurii ND132

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