Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-Producing Escherichia coli O145:H28
Abstract
Shiga toxin-producing Escherichia coli (STEC) O145:H28 can cause severe disease in humans and is a predominant serotype in STEC O145 environmental isolates. Here, comparative genomics was applied to a set of clinical and environmental strains to systematically evaluate the pathogenicity potential in environmental strains. While the core genes-based tree separated all O145:H28 strains from the non O145:H28 reference strains, it failed to segregate environmental strains from the clinical. In contrast, the accessory genes-based tree placed all clinical strains in the same clade regardless of their genotypes or serotypes, apart from the environmental strains. Loss-of-function mutations were common in the virulence genes examined, with a high frequency in genes related to adherence, autotransporters, and the type three secretion system. Distinct differences in pathogenicity islands LEE, OI-122, and OI-57, the acid fitness island, and the tellurite resistance island were detected between the O145:H28 and reference strains. A great amount of genetic variation was detected in O145:H28, which was mainly attributed to deletions, insertions, and gene acquisition at several chromosomal “hot spots”. Finally, our study demonstrated a distinct virulence gene repertoire among the STEC O145:H28 strains originating from the same geographical region and revealed unforeseen contributions of loss-of-function mutations to virulence evolution andmore »
- Authors:
-
[1];
[2];
[2]
- US Dept. of Agriculture (USDA), Albany, CA (United States). Western Regional Research Center
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Publication Date:
- Research Org.:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDA; USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1968211
- Report Number(s):
- LA-UR-23-23078
Journal ID: ISSN 2076-2607
- Grant/Contract Number:
- 89233218CNA000001
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Microorganisms
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 5; Journal ID: ISSN 2076-2607
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biological science; Shiga toxin-producing Escherichia coli (STEC); pangenome; pathogenicity islands; virulence genes
Citation Formats
Carter, Michelle Qiu, Laniohan, Nicole, Lo, Chien-Chi, and Chain, Patrick G. Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-Producing Escherichia coli O145:H28. United States: N. p., 2022.
Web. doi:10.3390/microorganisms10050866.
Carter, Michelle Qiu, Laniohan, Nicole, Lo, Chien-Chi, & Chain, Patrick G. Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-Producing Escherichia coli O145:H28. United States. https://doi.org/10.3390/microorganisms10050866
Carter, Michelle Qiu, Laniohan, Nicole, Lo, Chien-Chi, and Chain, Patrick G. Thu .
"Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-Producing Escherichia coli O145:H28". United States. https://doi.org/10.3390/microorganisms10050866. https://www.osti.gov/servlets/purl/1968211.
@article{osti_1968211,
title = {Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-Producing Escherichia coli O145:H28},
author = {Carter, Michelle Qiu and Laniohan, Nicole and Lo, Chien-Chi and Chain, Patrick G.},
abstractNote = {Shiga toxin-producing Escherichia coli (STEC) O145:H28 can cause severe disease in humans and is a predominant serotype in STEC O145 environmental isolates. Here, comparative genomics was applied to a set of clinical and environmental strains to systematically evaluate the pathogenicity potential in environmental strains. While the core genes-based tree separated all O145:H28 strains from the non O145:H28 reference strains, it failed to segregate environmental strains from the clinical. In contrast, the accessory genes-based tree placed all clinical strains in the same clade regardless of their genotypes or serotypes, apart from the environmental strains. Loss-of-function mutations were common in the virulence genes examined, with a high frequency in genes related to adherence, autotransporters, and the type three secretion system. Distinct differences in pathogenicity islands LEE, OI-122, and OI-57, the acid fitness island, and the tellurite resistance island were detected between the O145:H28 and reference strains. A great amount of genetic variation was detected in O145:H28, which was mainly attributed to deletions, insertions, and gene acquisition at several chromosomal “hot spots”. Finally, our study demonstrated a distinct virulence gene repertoire among the STEC O145:H28 strains originating from the same geographical region and revealed unforeseen contributions of loss-of-function mutations to virulence evolution and genetic diversification in STEC.},
doi = {10.3390/microorganisms10050866},
journal = {Microorganisms},
number = 5,
volume = 10,
place = {United States},
year = {Thu Apr 21 00:00:00 EDT 2022},
month = {Thu Apr 21 00:00:00 EDT 2022}
}
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