Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5′-monophosphate as the biological substrate

Hung, Shr-Hau; Elliott, Gregory I.; Ramkumar, Thakku R.; Burtnyak, Lyubomyr; McGrenaghan, Callum J.; Alkuzweny, Sana; Quaiyum, Samia; Iwata-Reuyl, Dirk; Pan, Xiaobei; Green, Brian D.; Kelly, Vincent P.; de Crécy-Lagard, Valérie; Swairjo, Manal A.

doi:10.1093/nar/gkac1231

Title: Structural basis of Qng1-mediated salvage of the micronutrient queuine from queuosine-5′-monophosphate as the biological substrate

Journal Article · Thu Jan 05 00:00:00 UTC 2023 · Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gkac1231 · OSTI ID:1908287

;

; Burtnyak, Lyubomyr; McGrenaghan, Callum J.; Alkuzweny, Sana; Quaiyum, Samia;

;

; Green, Brian D.;

;

Abstract Eukaryotic life benefits from—and ofttimes critically relies upon—the de novo biosynthesis and supply of vitamins and micronutrients from bacteria. The micronutrient queuosine (Q), derived from diet and/or the gut microbiome, is used as a source of the nucleobase queuine, which once incorporated into the anticodon of tRNA contributes to translational efficiency and accuracy. Here, we report high-resolution, substrate-bound crystal structures of the Sphaerobacter thermophilus queuine salvage protein Qng1 (formerly DUF2419) and of its human ortholog QNG1 (C9orf64), which together with biochemical and genetic evidence demonstrate its function as the hydrolase releasing queuine from queuosine-5′-monophosphate as the biological substrate. We also show that QNG1 is highly expressed in the liver, with implications for Q salvage and recycling. The essential role of this family of hydrolases in supplying queuine in eukaryotes places it at the nexus of numerous (patho)physiological processes associated with queuine deficiency, including altered metabolism, proliferation, differentiation and cancer progression.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: National Institutes of Health (NIH); Science Foundation Ireland; USDOE

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1908287

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 2 Vol. 51; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (74)

Mechanism and Substrate Specificity of tRNA-Guanine Transglycosylases (TGTs): tRNA-Modifying Enzymes from the Three Different Kingdoms of Life Share a Common Catalytic Mechanism Stengl, Bernhard; Reuter, Klaus; Klebe, Gerhard ChemBioChem, Vol. 6, Issue 11 https://doi.org/10.1002/cbic.200500063	journal	October 2005
Delineation of the minimal commonly deleted segment and identification of candidate tumor-suppressor genes in del(9q) acute myeloid leukemia Sweetser, David A.; Peniket, Andrew J.; Haaland, Christina Genes, Chromosomes and Cancer, Vol. 44, Issue 3 https://doi.org/10.1002/gcc.20236	journal	January 2005
Solution structure of the nucleotide hydrolase BlsM: Implication of its substrate specificity Kang, Minhee; Doddapaneni, Kiran; Sarni, Samantha Protein Science, Vol. 29, Issue 8 https://doi.org/10.1002/pro.3812	journal	July 2020
Effects of a Diet Deficient in Tyrosine and Queuine on Germfree Mice Marks, T.; Farkas, W. R. Biochemical and Biophysical Research Communications, Vol. 230, Issue 2 https://doi.org/10.1006/bbrc.1996.5768	journal	January 1997
Queuosine Modification of tRNA: A Case for Convergent Evolution Morris, Rana C.; Elliott, Mark S. Molecular Genetics and Metabolism, Vol. 74, Issue 1-2 https://doi.org/10.1006/mgme.2001.3216	journal	September 2001
Queuine is incorporated into brain transfer RNA Siard, T. J.; Katze, J. R.; Farkas, W. R. Neurochemical Research, Vol. 14, Issue 11 https://doi.org/10.1007/BF00965624	journal	November 1989
A homozygous truncating mutation in PUS3 expands the role of tRNA modification in normal cognition Shaheen, Ranad; Han, Lu; Faqeih, Eissa Human Genetics, Vol. 135, Issue 7 https://doi.org/10.1007/s00439-016-1665-7	journal	April 2016
Salvage of the nucleic acid base queuine from queuine-containing tRNA by animal cells Gündüz, Ufuk; Katze, Jon R. Biochemical and Biophysical Research Communications, Vol. 109, Issue 1 https://doi.org/10.1016/0006-291X(82)91579-0	journal	November 1982
Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus White, Bradley N.; Teneb, G. M.; Holden, Jeannette Journal of Molecular Biology, Vol. 74, Issue 4 https://doi.org/10.1016/0022-2836(73)90054-5	journal	March 1973
Variations in tRNA modifications, particularly of their queuine content in higher eukaryotes. Its relation to malignancy grading Dirheimer, G.; Baranowski, W.; Keith, G. Biochimie, Vol. 77, Issue 1-2 https://doi.org/10.1016/0300-9084(96)88111-9	journal	January 1995
Queuine salvage in mammalian cells. Evidence that queuine is generated from queuosine 5'-phosphate. Gündüz, U.; Katze, J. R. Journal of Biological Chemistry, Vol. 259, Issue 2 https://doi.org/10.1016/S0021-9258(17)43573-3	journal	January 1984
Structure of an amniotic fluid component, 7-(4,5-cis-dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanine (queuine), a substrate for tRNA: guanine transglycosylase. Crain, P. F.; Sethi, S. K.; Katze, J. R. Journal of Biological Chemistry, Vol. 255, Issue 18 https://doi.org/10.1016/S0021-9258(18)43509-0	journal	September 1980
Purification and characterization of a Saccharomyces cerevisiae exoribonuclease which yields 5'-mononucleotides by a 5' leads to 3' mode of hydrolysis. Stevens, A. Journal of Biological Chemistry, Vol. 255, Issue 7 https://doi.org/10.1016/S0021-9258(19)85855-6	journal	April 1980
Preparation of selenomethionyl proteins for phase determination Doublié, Sylvie Methods in Enzymology https://doi.org/10.1016/S0076-6879(97)76075-0 p. 523-530	book	January 1997
Recent advances in the structural mechanisms of DNA glycosylases Brooks, Sonja C.; Adhikary, Suraj; Rubinson, Emily H. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1834, Issue 1 https://doi.org/10.1016/j.bbapap.2012.10.005	journal	January 2013
Queuosine salvage in fission yeast by Qng1-mediated hydrolysis to queuine Patel, Bhargesh Indravadan; Heiss, Matthias; Samel-Pommerencke, Anke Biochemical and Biophysical Research Communications, Vol. 624 https://doi.org/10.1016/j.bbrc.2022.07.104	journal	October 2022
Structural Characterization of the Mammalian Deoxynucleotide N-Hydrolase Rcl and Its Stabilizing Interactions with Two Inhibitors Yang, Yinshan; Padilla, André; Zhang, Chi Journal of Molecular Biology, Vol. 394, Issue 3 https://doi.org/10.1016/j.jmb.2009.10.004	journal	December 2009
Structural Insights into the Mechanism of Base Excision by MBD4 Pidugu, Lakshmi S.; Bright, Hilary; Lin, Wen-Jen Journal of Molecular Biology, Vol. 433, Issue 15 https://doi.org/10.1016/j.jmb.2021.167097	journal	July 2021
Structural and functional characterization of two unusual endonuclease III enzymes from Deinococcus radiodurans Sarre, Aili; Ökvist, Mats; Klar, Tobias Journal of Structural Biology, Vol. 191, Issue 2 https://doi.org/10.1016/j.jsb.2015.05.009	journal	August 2015
(p)ppGpp Regulates a Bacterial Nucleosidase by an Allosteric Two-Domain Switch Zhang, Yong Everett; Bærentsen, René Lysdal; Fuhrer, Tobias Molecular Cell, Vol. 74, Issue 6 https://doi.org/10.1016/j.molcel.2019.03.035	journal	June 2019
Catalysis by nucleoside hydrolases Versées, Wim; Steyaert, Jan Current Opinion in Structural Biology, Vol. 13, Issue 6 https://doi.org/10.1016/j.sbi.2003.10.002	journal	December 2003
A DNA Glycosylase from Pyrobaculum aerophilum with an 8-Oxoguanine Binding Mode and a Noncanonical Helix-Hairpin-Helix Structure Lingaraju, Gondichatnahalli M.; Sartori, Alessandro A.; Kostrewa, Dirk Structure, Vol. 13, Issue 1 https://doi.org/10.1016/j.str.2004.10.011	journal	January 2005
Separation-of-Function Mutants Unravel the Dual-Reaction Mode of Human 8-Oxoguanine DNA Glycosylase Dalhus, Bjørn; Forsbring, Monika; Helle, Ina Høydal Structure, Vol. 19, Issue 1 https://doi.org/10.1016/j.str.2010.09.023	journal	January 2011
Microbes and Monoamines: Potential Neuropsychiatric Consequences of Dysbiosis Skolnick, Stephen D.; Greig, Nigel H. Trends in Neurosciences, Vol. 42, Issue 3 https://doi.org/10.1016/j.tins.2018.12.005	journal	March 2019
Kinetic Isotope Effect Studies of the Reaction Catalyzed by Uracil DNA Glycosylase: Evidence for an Oxocarbenium Ion−Uracil Anion Intermediate ^† Werner, R. Marshall; Stivers, James T. Biochemistry, Vol. 39, Issue 46 https://doi.org/10.1021/bi0018178	journal	November 2000
Evidence that the nucleic acid base queuine is incorporated intact into tRNA by animal cells Katze, Jon R.; Gunduz, Ufuk; Smith, David L. Biochemistry, Vol. 23, Issue 6 https://doi.org/10.1021/bi00301a022	journal	March 1984
Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine Kasai, H.; Ohashi, Z.; Harada, F. Biochemistry, Vol. 14, Issue 19 https://doi.org/10.1021/bi00690a008	journal	September 1975
Diversity of Archaeosine Synthesis in Crenarchaeota Phillips, Gabriela; Swairjo, Manal A.; Gaston, Kirk W. ACS Chemical Biology, Vol. 7, Issue 2 https://doi.org/10.1021/cb200361w	journal	November 2011
Plant, Animal, and Fungal Micronutrient Queuosine Is Salvaged by Members of the DUF2419 Protein Family Zallot, Rémi; Brochier-Armanet, Céline; Gaston, Kirk W. ACS Chemical Biology, Vol. 9, Issue 8 https://doi.org/10.1021/cb500278k	journal	June 2014
The structure of Q* nucleoside isolated from rabbit liver transfer ribonucleic acid Kasai, H.; Nakanishi, K.; Macfarlane, R. D. Journal of the American Chemical Society, Vol. 98, Issue 16 https://doi.org/10.1021/ja00432a071	journal	August 1976
Total synthesis of optically pure nucleoside Q. Determination of absolute configuration of natural nucleoside Q Ohgi, Tadaaki; Kondo, Tadao; Goto, Toshio Journal of the American Chemical Society, Vol. 101, Issue 13 https://doi.org/10.1021/ja00507a032	journal	June 1979
Transition-State Analysis of the DNA Repair Enzyme MutY McCann, Joe A. B.; Berti, Paul J. Journal of the American Chemical Society, Vol. 130, Issue 17 https://doi.org/10.1021/ja711363s	journal	April 2008
Structural basis for recognition and repair of the endogenous mutagen 8-oxoguanine in DNA Bruner, Steven D.; Norman, Derek P. G.; Verdine, Gregory L. Nature, Vol. 403, Issue 6772 https://doi.org/10.1038/35002510	journal	February 2000
Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe Kim, Dong-Uk; Hayles, Jacqueline; Kim, Dongsup Nature Biotechnology, Vol. 28, Issue 6 https://doi.org/10.1038/nbt.1628	journal	May 2010
Incomplete DNA methylation underlies a transcriptional memory of somatic cells in human iPS cells Ohi, Yuki; Qin, Han; Hong, Chibo Nature Cell Biology, Vol. 13, Issue 5 https://doi.org/10.1038/ncb2239	journal	April 2011
Complete chemical structures of human mitochondrial tRNAs Suzuki, Takeo; Yashiro, Yuka; Kikuchi, Ittoku Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-18068-6	journal	August 2020
Highly accurate protein structure prediction with AlphaFold Jumper, John; Evans, Richard; Pritzel, Alexander Nature https://doi.org/10.1038/s41586-021-03819-2	journal	July 2021
Structural basis for cytokinin production by LOG from Corynebacterium glutamicum Seo, Hogyun; Kim, Sangwoo; Sagong, Hye-Young Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep31390	journal	August 2016
Prolonging healthy aging: Longevity vitamins and proteins Ames, Bruce N. Proceedings of the National Academy of Sciences, Vol. 115, Issue 43 https://doi.org/10.1073/pnas.1809045115	journal	October 2018
Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens Yuan, Yifeng; Zallot, Rémi; Grove, Tyler L. Proceedings of the National Academy of Sciences, Vol. 116, Issue 38 https://doi.org/10.1073/pnas.1909604116	journal	September 2019
Queuosine Deficiency in Eukaryotes Compromises Tyrosine Production through Increased Tetrahydrobiopterin Oxidation Rakovich, Tatsiana; Boland, Coilin; Bernstein, Ilana Journal of Biological Chemistry, Vol. 286, Issue 22 https://doi.org/10.1074/jbc.M111.219576	journal	April 2011
Deazaguanine derivatives, examples of crosstalk between RNA and DNA modification pathways Hutinet, Geoffrey; Swarjo, Manal A.; de Crécy-Lagard, Valérie RNA Biology, Vol. 14, Issue 9 https://doi.org/10.1080/15476286.2016.1265200	journal	December 2016
Structural and functional insights into human tRNA guanine transgylcosylase Sievers, Katharina; Welp, Luisa; Urlaub, Henning RNA Biology, Vol. 18, Issue sup1 https://doi.org/10.1080/15476286.2021.1950980	journal	July 2021
Affinity electrophoresis for monitoring terminal phosphorylation and the presence of queuosine in RNA. Application of polyacrylamide containing a covalently bound boronic acid Igloi, Gabar L.; Kössel, Hans Nucleic Acids Research, Vol. 13, Issue 19 https://doi.org/10.1093/nar/13.19.6881	journal	January 1985
C to U editing and modifications during the maturation of the mitochondrial tRNA^ASPin marsupials Mörl, Mario; Dörner, Marion; Pääbo, Svante Nucleic Acids Research, Vol. 23, Issue 17 https://doi.org/10.1093/nar/23.17.3380	journal	January 1995
Isolation of mammalian tRNA^Aspand tRNA^Tyrby lectin-Sepharose affinity column chromatography Okada, Norihiro; Shindo-Okada, Nobuko; Nishimura, Susumu Nucleic Acids Research, Vol. 4, Issue 2 https://doi.org/10.1093/nar/4.2.415	journal	January 1977
Enzymatic synthesis of Q^*nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver Okada, Norihiro; Nishimura, Susumu Nucleic Acids Research, Vol. 4, Issue 8 https://doi.org/10.1093/nar/4.8.2931	journal	January 1977
The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity Fergus, Claire; Al-qasem, Mashael; Cotter, Michelle Nucleic Acids Research, Vol. 49, Issue 9 https://doi.org/10.1093/nar/gkab289	journal	May 2021
Preferential import of queuosine-modified tRNAs into Trypanosoma brucei mitochondrion is critical for organellar protein synthesis Kulkarni, Sneha; Rubio, Mary Anne T.; Hegedűsová, Eva Nucleic Acids Research, Vol. 49, Issue 14 https://doi.org/10.1093/nar/gkab567	journal	July 2021
Excision of thymine and 5-hydroxymethyluracil by the MBD4 DNA glycosylase domain: structural basis and implications for active DNA demethylation Hashimoto, Hideharu; Zhang, Xing; Cheng, Xiaodong Nucleic Acids Research, Vol. 40, Issue 17 https://doi.org/10.1093/nar/gks628	journal	June 2012
Structure of theN-glycosidase MilB in complex with hydroxymethyl CMP reveals its Arg23 specifically recognizes the substrate and controls its entry Zhao, Gong; Wu, Geng; Zhang, Yan Nucleic Acids Research, Vol. 42, Issue 12 https://doi.org/10.1093/nar/gku486	journal	June 2014
Structure and stereochemistry of the base excision repair glycosylase MutY reveal a mechanism similar to retaining glycosidases Woods, Ryan D.; O'Shea, Valerie L.; Chu, Aurea Nucleic Acids Research, Vol. 44, Issue 2 https://doi.org/10.1093/nar/gkv1469	journal	December 2015
Queuine links translational control in eukaryotes to a micronutrient from bacteria Müller, Martin; Legrand, Carine; Tuorto, Francesca Nucleic Acids Research, Vol. 47, Issue 7 https://doi.org/10.1093/nar/gkz063	journal	February 2019
Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5′–3′ exonucleases Rat1 and Xrn1 Chernyakov, Irina; Whipple, Joseph M.; Kotelawala, Lakmal Genes & Development, Vol. 22, Issue 10 https://doi.org/10.1101/gad.1654308	journal	April 2008
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes Minor, Wladek; Cymborowski, Marcin; Otwinowski, Zbyszek Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8 https://doi.org/10.1107/S0907444906019949	journal	July 2006
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V. Acta Crystallographica Section D Biological Crystallography, Vol. 64, Issue 1 https://doi.org/10.1107/S090744490705024X	journal	December 2007
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
REFMAC 5 for the refinement of macromolecular crystal structures Murshudov, Garib N.; Skubák, Pavol; Lebedev, Andrey A. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444911001314	journal	March 2011
Towards automated crystallographic structure refinement with phenix.refine Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4 https://doi.org/10.1107/S0907444912001308	journal	March 2012
SOLVE and RESOLVE: automated structure solution, density modification and model building Terwilliger, Thomas Journal of Synchrotron Radiation, Vol. 11, Issue 1 https://doi.org/10.1107/S0909049503023938	journal	November 2003
Recent developments in the PHENIX software for automated crystallographic structure determination Adams, Paul D.; Gopal, Kreshna; Grosse-Kunstleve, Ralf W. Journal of Synchrotron Radiation, Vol. 11, Issue 1 https://doi.org/10.1107/S0909049503024130	journal	November 2003
The nutrient factor queuine protects HeLa cells from hypoxic stress and improves metabolic adaptation to oxygen availability Reisser, Thomas; Langgut, Werner; Kersten, Helga European Journal of Biochemistry, Vol. 221, Issue 3 https://doi.org/10.1111/j.1432-1033.1994.tb18814.x	journal	May 1994
Nuclear Functions Charge Ahead Hopper, Anita K. Science, Vol. 282, Issue 5396 https://doi.org/10.1126/science.282.5396.2003	journal	December 1998
Novel salvage of queuine from queuosine and absence of queuine synthesis in Chlorella pyrenoidosa and Chlamydomonas reinhardtii. Kirtland, G. M.; Morris, T. D.; Moore, P. H. Journal of Bacteriology, Vol. 170, Issue 12 https://doi.org/10.1128/jb.170.12.5633-5641.1988	journal	December 1988
A Nutrient-Driven tRNA Modification Alters Translational Fidelity and Genome-wide Protein Coding across an Animal Genus Zaborske, John M.; Bauer DuMont, Vanessa L.; Wallace, Edward W. J. PLoS Biology, Vol. 12, Issue 12 https://doi.org/10.1371/journal.pbio.1002015	journal	December 2014
Queuine, a bacterial-derived hypermodified nucleobase, shows protection in in vitro models of neurodegeneration Richard, Patricia; Kozlowski, Lucie; Guillorit, Hélène PLOS ONE, Vol. 16, Issue 8 https://doi.org/10.1371/journal.pone.0253216	journal	August 2021
Queuosine‐modified tRNAs confer nutritional control of protein translation Tuorto, Francesca; Legrand, Carine; Cirzi, Cansu The EMBO Journal, Vol. 37, Issue 18 https://doi.org/10.15252/embj.201899777	journal	August 2018
Queuine Salvaging in the Human Parasite Entamoeba histolytica Sarid, Lotem; Sun, Jingjing; Chittrakanwong, Jurairat Cells, Vol. 11, Issue 16 https://doi.org/10.3390/cells11162509	journal	August 2022
Queuine Micronutrient Deficiency Promotes Warburg Metabolism and Reversal of the Mitochondrial ATP Synthase in Hela Cells Hayes, Patti; Fergus, Claire; Ghanim, Magda Nutrients, Vol. 12, Issue 3 https://doi.org/10.3390/nu12030871	journal	March 2020
The Queuine Micronutrient: Charting a Course from Microbe to Man Fergus, Claire; Barnes, Dominic; Alqasem, Mashael Nutrients, Vol. 7, Issue 4 https://doi.org/10.3390/nu7042897	journal	April 2015

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