Whole-Genome SNP Analysis Identifies Putative Mycobacterium bovis Transmission Clusters in Livestock and Wildlife in Catalonia, Spain

Perea, Claudia; Ciaravino, Giovanna; Stuber, Tod; Thacker, Tyler C.; Robbe-Austerman, Suelee; Allepuz, Alberto; Val, Bernat Pérez de

doi:10.3390/microorganisms9081629

Title: Whole-Genome SNP Analysis Identifies Putative Mycobacterium bovis Transmission Clusters in Livestock and Wildlife in Catalonia, Spain

Journal Article · 30 July 2021 · Microorganisms

DOI: https://doi.org/10.3390/microorganisms9081629 · OSTI ID:1904918

^[1]; Ciaravino, Giovanna;

^[2]; Thacker, Tyler C. ^[2];

^[2]; Allepuz, Alberto ^[3];

^[4]

US Dept. of Agriculture (USDA), Ames, IA (United States). National Veterinary Services Laboratories; OSTI
US Dept. of Agriculture (USDA), Ames, IA (United States). National Veterinary Services Laboratories
Univ. Autonoma de Barcelona (Spain); Centre de Recerca en Sanitat Animal (CReSA), Bellaterra (Spain); OIE Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe, Bellaterra (Spain)
Centre de Recerca en Sanitat Animal (CReSA), Bellaterra (Spain); OIE Collaborating Centre for the Research and Control of Emerging and Re-Emerging Swine Diseases in Europe, Bellaterra (Spain)

The high-resolution WGS analyses of MTBC strains have provided useful insight for determining sources of infection for animal tuberculosis. In Spain, tuberculosis in livestock is caused by Mycobacterium bovis and Mycobacterium caprae, where wildlife reservoirs play an important role. We analyzed a set of 125 M. bovis isolates obtained from livestock and wildlife from Catalonia to investigate strain diversity and identify possible sources and/or causes of infection. Whole-genome SNP profiles were used for phylogenetic reconstruction and pairwise SNP distance analysis. Additionally, SNPs were investigated to identify virulence and antimicrobial resistance factors to investigate clade-specific associations. Putative transmission clusters (≤12 SNPs) were identified, and associated epidemiological metadata were used to determine possible explanatory factors for transmission. M. bovis distribution was heterogeneous, with 7 major clades and 21 putative transmission clusters. In order of importance, the explanatory factors associated were proximity and neighborhood, residual infection, livestock-wildlife interaction, shared pasture, and movement. Genes related to lipid transport and metabolism showed the highest number of SNPs. All isolates were pyrazinamide resistant, and five were additionally resistant to isoniazid, but no clade-specific associations could be determined. Our findings highlight the importance of high-resolution molecular surveillance to monitor bovine tuberculosis dynamics in a low-prevalence setting.

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Research Organization:: Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: Ministerio de Ciencia e Innovación; USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 1904918

Journal Information:: Microorganisms, Journal Name: Microorganisms Journal Issue: 8 Vol. 9; ISSN 2076-2607; ISSN MICRKN

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

References (77)

Short-Course Chemotherapy for Pulmonary Infection due to Mycobacterium bovis O'Donohue, Walter J. Archives of Internal Medicine, Vol. 145, Issue 4 https://doi.org/10.1001/archinte.1985.00360040135029	journal	April 1985
Most likely causes of infection and risk factors for tuberculosis in Spanish cattle herds Ciaravino, Giovanna; Laranjo‐González, Minerva; Casal, Jordi Veterinary Record, Vol. 189, Issue 2 https://doi.org/10.1002/vetr.140	journal	February 2021
A case-control study on the association of selected risk factors with the occurrence of bovine tuberculosis in the Republic of Ireland Griffin, John M.; Martin, S. Wayne; Thorburn, Meg A. Preventive Veterinary Medicine, Vol. 27, Issue 3-4 https://doi.org/10.1016/0167-5877(95)00571-4	journal	July 1996
The transmission of Mycobacterium bovis infection to cattle Phillips, C. J. C.; Foster, C. R. W.; Morris, P. A. Research in Veterinary Science, Vol. 74, Issue 1 https://doi.org/10.1016/S0034-5288(02)00145-5	journal	February 2003
Whole-genome sequencing to delineate Mycobacterium tuberculosis outbreaks: a retrospective observational study Walker, Timothy M.; Ip, Camilla LC; Harrell, Ruth H. The Lancet Infectious Diseases, Vol. 13, Issue 2 https://doi.org/10.1016/S1473-3099(12)70277-3	journal	February 2013
Association patterns of visually-observed cattle on Montana, USA foothill rangelands Stephenson, Mitchell B.; Bailey, Derek W.; Jensen, Delyn Applied Animal Behaviour Science, Vol. 178 https://doi.org/10.1016/j.applanim.2016.02.007	journal	May 2016
Use of bacterial whole-genome sequencing to investigate local persistence and spread in bovine tuberculosis Trewby, Hannah; Wright, David; Breadon, Eleanor L. Epidemics, Vol. 14 https://doi.org/10.1016/j.epidem.2015.08.003	journal	March 2016
Assessing the variability in transmission of bovine tuberculosis within Spanish cattle herds Ciaravino, G.; García-Saenz, A.; Cabras, S. Epidemics, Vol. 23 https://doi.org/10.1016/j.epidem.2018.01.003	journal	June 2018
European 1: A globally important clonal complex of Mycobacterium bovis Smith, Noel H.; Berg, Stefan; Dale, James Infection, Genetics and Evolution, Vol. 11, Issue 6 https://doi.org/10.1016/j.meegid.2011.04.027	journal	August 2011
European 2 – A clonal complex of Mycobacterium bovis dominant in the Iberian Peninsula Rodriguez-Campos, Sabrina; Schürch, Anita C.; Dale, James Infection, Genetics and Evolution, Vol. 12, Issue 4 https://doi.org/10.1016/j.meegid.2011.09.004	journal	June 2012
The global distribution and phylogeography of Mycobacterium bovis clonal complexes Smith, Noel H. Infection, Genetics and Evolution, Vol. 12, Issue 4 https://doi.org/10.1016/j.meegid.2011.09.007	journal	June 2012
The complete genome sequence of Mycobacterium bovis Mb3601, a SB0120 spoligotype strain representative of a new clonal group Branger, Maxime; Loux, Valentin; Cochard, Thierry Infection, Genetics and Evolution, Vol. 82 https://doi.org/10.1016/j.meegid.2020.104309	journal	August 2020
Analysis of the spatial variation of Bovine tuberculosis disease risk in Spain (2006–2009) Allepuz, A.; Casal, J.; Napp, S. Preventive Veterinary Medicine, Vol. 100, Issue 1 https://doi.org/10.1016/j.prevetmed.2011.02.012	journal	June 2011
The importance of ‘neighbourhood’ in the persistence of bovine tuberculosis in Irish cattle herds White, Paul. W.; Martin, S. Wayne; De Jong, Mart C. M. Preventive Veterinary Medicine, Vol. 110, Issue 3-4 https://doi.org/10.1016/j.prevetmed.2013.02.012	journal	July 2013
Estimation of the individual slaughterhouse surveillance sensitivity for bovine tuberculosis in Catalonia (North-Eastern Spain) Garcia-Saenz, A.; Napp, S.; Lopez, S. Preventive Veterinary Medicine, Vol. 121, Issue 3-4 https://doi.org/10.1016/j.prevetmed.2015.08.008	journal	October 2015
Evaluation of the effectiveness of the surveillance system for tuberculosis in cattle in Spain Napp, Sebastian; Ciaravino, Giovanna; Pérez de Val, Bernat Preventive Veterinary Medicine, Vol. 173 https://doi.org/10.1016/j.prevetmed.2019.104805	journal	December 2019
An epidemiological evaluation of Mycobacterium bovis infections in wild game animals of the Spanish Mediterranean ecosystem Parra, A.; García, A.; Inglis, N. F. Research in Veterinary Science, Vol. 80, Issue 2 https://doi.org/10.1016/j.rvsc.2005.05.010	journal	April 2006
Understanding the relationship between Mycobacterium bovis spoligotypes from cattle in Latin American Countries Zumárraga, M. J.; Arriaga, C.; Barandiaran, S. Research in Veterinary Science, Vol. 94, Issue 1 https://doi.org/10.1016/j.rvsc.2012.07.012	journal	February 2013
Overview and phylogeny of Mycobacterium tuberculosis complex organisms: Implications for diagnostics and legislation of bovine tuberculosis Rodriguez-Campos, Sabrina; Smith, Noel H.; Boniotti, Maria B. Research in Veterinary Science, Vol. 97 https://doi.org/10.1016/j.rvsc.2014.02.009	journal	October 2014
Transmission of tuberculosis caused by Mycobacterium caprae between dairy sheep and goats Vidal, Enric; Grasa, Miriam; Perálvarez, Tania Small Ruminant Research, Vol. 158 https://doi.org/10.1016/j.smallrumres.2017.11.010	journal	January 2018
Lesions associated with Mycobacterium tuberculosis complex infection in the European wild boar Martín-Hernando, Maria Paz; Höfle, Ursula; Vicente, Joaquin Tuberculosis, Vol. 87, Issue 4 https://doi.org/10.1016/j.tube.2007.02.003	journal	July 2007
Genotyping and rifampicin and isoniazid resistance in Mycobacterium bovis strains isolated from the lymph nodes of slaughtered cattle Franco, Marília Masello Junqueira; Ribeiro, Márcio Garcia; Pavan, Fernando Rogério Tuberculosis, Vol. 104 https://doi.org/10.1016/j.tube.2017.02.006	journal	May 2017
Towards eradication of bovine tuberculosis in the European Union Reviriego Gordejo, F. J.; Vermeersch, J. P. Veterinary Microbiology, Vol. 112, Issue 2-4 https://doi.org/10.1016/j.vetmic.2005.11.034	journal	February 2006
Evidence of the role of European wild boar as a reservoir of Mycobacterium tuberculosis complex Naranjo, Victoria; Gortazar, Christian; Vicente, Joaquín Veterinary Microbiology, Vol. 127, Issue 1-2 https://doi.org/10.1016/j.vetmic.2007.10.002	journal	February 2008
High spoligotype diversity within a Mycobacterium bovis population: Clues to understanding the demography of the pathogen in Europe Rodríguez, Sabrina; Romero, Beatriz; Bezos, Javier Veterinary Microbiology, Vol. 141, Issue 1-2 https://doi.org/10.1016/j.vetmic.2009.08.007	journal	February 2010
Progress in the control of bovine tuberculosis in Spanish wildlife Gortazar, Christian; Vicente, Joaquín; Boadella, Mariana Veterinary Microbiology, Vol. 151, Issue 1-2 https://doi.org/10.1016/j.vetmic.2011.02.041	journal	July 2011
An Impossible Undertaking: The Eradication of Bovine Tuberculosis in the United States Olmstead, Alan L.; Rhode, Paul W. The Journal of Economic History, Vol. 64, Issue 3 https://doi.org/10.1017/S0022050704002955	journal	September 2004
Pyrazinamide Resistance Is Caused by Two Distinct Mechanisms: Prevention of Coenzyme A Depletion and Loss of Virulence Factor Synthesis Gopal, Pooja; Yee, Michelle; Sarathy, Jickky ACS Infectious Diseases, Vol. 2, Issue 9 https://doi.org/10.1021/acsinfecdis.6b00070	journal	August 2016
Aspartate decarboxylase (PanD) as a new target of pyrazinamide in Mycobacterium tuberculosis Shi, Wanliang; Chen, Jiazhen; Feng, Jie Emerging Microbes & Infections, Vol. 3, Issue 1 https://doi.org/10.1038/emi.2014.61	journal	January 2014
Integrative genomics viewer Robinson, James T.; Thorvaldsdóttir, Helga; Winckler, Wendy Nature Biotechnology, Vol. 29, Issue 1 https://doi.org/10.1038/nbt.1754	journal	January 2011
Long-term molecular surveillance provides clues on a cattle origin for Mycobacterium bovis in Portugal Reis, Ana C.; Tenreiro, Rogério; Albuquerque, Teresa Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-77713-8	journal	November 2020
Exploring Euro-African pasts through an analysis of Spanish colonial practices in Africa (Morocco and Spanish Guinea) Aixelà-Cabré, Yolanda Canadian Journal of African Studies / Revue canadienne des études africaines, Vol. 51, Issue 1 https://doi.org/10.1080/00083968.2016.1276848	journal	January 2017
Toward eradication: the effect ofMycobacterium bovisinfection in wildlife on the evolution and future direction of bovine tuberculosis management in New Zealand Livingstone, Pg; Hancox, N.; Nugent, G. New Zealand Veterinary Journal, Vol. 63, Issue sup1 https://doi.org/10.1080/00480169.2014.971082	journal	March 2015
Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation Letunic, I.; Bork, P. Bioinformatics, Vol. 23, Issue 1 https://doi.org/10.1093/bioinformatics/btl529	journal	October 2006
Fast and accurate short read alignment with Burrows-Wheeler transform Li, H.; Durbin, R. Bioinformatics, Vol. 25, Issue 14 https://doi.org/10.1093/bioinformatics/btp324	journal	May 2009
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies Stamatakis, Alexandros Bioinformatics, Vol. 30, Issue 9 https://doi.org/10.1093/bioinformatics/btu033	journal	January 2014
An African origin for Mycobacterium bovis Loiseau, Chloé; Menardo, Fabrizio; Aseffa, Abraham Evolution, Medicine, and Public Health, Vol. 2020, Issue 1 https://doi.org/10.1093/emph/eoaa005	journal	January 2020
PointFinder: a novel web tool for WGS-based detection of antimicrobial resistance associated with chromosomal point mutations in bacterial pathogens Zankari, Ea; Allesøe, Rosa; Joensen, Katrine G. Journal of Antimicrobial Chemotherapy, Vol. 72, Issue 10 https://doi.org/10.1093/jac/dkx217	journal	July 2017
Elevation of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to species rank as Mycobacterium caprae comb. nov., sp. nov. Aranaz, A. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 53, Issue 6 https://doi.org/10.1099/ijs.0.02532-0	journal	November 2003
Detection and identification of multiple mycobacterial pathogens by DNA amplification in a single tube. Wilton, S.; Cousins, D. Genome Research, Vol. 1, Issue 4 https://doi.org/10.1101/gr.1.4.269	journal	May 1992
Domestication of cattle: Two or three events? Pitt, Daniel; Sevane, Natalia; Nicolazzi, Ezequiel L. Evolutionary Applications, Vol. 12, Issue 1 https://doi.org/10.1111/eva.12674	journal	July 2018
Environmental Presence ofMycobacterium tuberculosisComplex in Aggregation Points at the Wildlife/Livestock Interface Barasona, J. A.; Vicente, J.; Díez-Delgado, I. Transboundary and Emerging Diseases, Vol. 64, Issue 4 https://doi.org/10.1111/tbed.12480	journal	February 2016
The spread of bovine tuberculosis in Canadian shared pastures: Data, model, and simulations Rossi, Gianluigi; Aubry, Pascale; Dubé, Caroline Transboundary and Emerging Diseases, Vol. 66, Issue 1 https://doi.org/10.1111/tbed.13066	journal	December 2018
Phylogenetic relationships investigation of Mycobacterium caprae strains from sympatric wild boar and goats based on whole genome sequencing Ciaravino, Giovanna; Vidal, Enric; Cortey, Martí Transboundary and Emerging Diseases, Vol. 68, Issue 3 https://doi.org/10.1111/tbed.13816	journal	September 2020
Cloning of an M. tuberculosis DNA fragment associated with entry and survival inside cells Arruda, S.; Bomfim, G.; Knights, R. Science, Vol. 261, Issue 5127 https://doi.org/10.1126/science.8367727	journal	September 1993
Molecular Basis of Rifampin and Isoniazid Resistance in Mycobacterium bovis Strains Isolated in Sardinia, Italy Sechi, L. A.; Zanetti, S.; Sanguinetti, M. Antimicrobial Agents and Chemotherapy, Vol. 45, Issue 6 https://doi.org/10.1128/AAC.45.6.1645-1648.2001	journal	June 2001
African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa Berg, Stefan; Garcia-Pelayo, M. Carmen; Müller, Borna Journal of Bacteriology, Vol. 193, Issue 3 https://doi.org/10.1128/JB.00750-10	journal	February 2011
African 1, an Epidemiologically Important Clonal Complex of Mycobacterium bovis Dominant in Mali, Nigeria, Cameroon, and Chad Müller, Borna; Hilty, Markus; Berg, Stefan Journal of Bacteriology, Vol. 191, Issue 6 https://doi.org/10.1128/JB.01590-08	journal	March 2009
Splitting of a Prevalent Mycobacterium bovis Spoligotype by Variable-Number Tandem-Repeat Typing Reveals High Heterogeneity in an Evolving Clonal Group Rodriguez-Campos, Sabrina; Navarro, Yurena; Romero, Beatriz Journal of Clinical Microbiology, Vol. 51, Issue 11 https://doi.org/10.1128/JCM.01271-13	journal	November 2013
Population Structure of Mycobacterium bovis in Germany: a Long-Term Study Using Whole-Genome Sequencing Combined with Conventional Molecular Typing Methods Kohl, Thomas A.; Kranzer, Katharina; Andres, Sönke Journal of Clinical Microbiology, Vol. 58, Issue 11 https://doi.org/10.1128/JCM.01573-20	journal	October 2020
Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: a tool for studying epidemiology of tuberculosis Aranaz, A.; Liébana, E.; Mateos, A. Journal of Clinical Microbiology, Vol. 34, Issue 11 https://doi.org/10.1128/jcm.34.11.2734-2740.1996	journal	November 1996
Evidence of goats acting as domestic reservoirs of bovine tuberculosis Napp, S.; Allepuz, A.; Mercader, I. Veterinary Record, Vol. 172, Issue 25 https://doi.org/10.1136/vr.101347	journal	June 2013
Lessons learned during the successful eradication of bovine tuberculosis from Australia More, S. J.; Radunz, B.; Glanville, R. J. Veterinary Record, Vol. 177, Issue 9 https://doi.org/10.1136/vr.103163	journal	September 2015
Herd-Level Risk Factors for Bovine Tuberculosis: A Literature Review Skuce, Robin A.; Allen, Adrian R.; McDowell, Stanley W. J. Veterinary Medicine International, Vol. 2012 https://doi.org/10.1155/2012/621210	journal	January 2012
Primary Isoniazid Resistance in Mycobacterium bovis Disease: A Prospect of Concern McLaughlin, Anne Marie; Gibbons, Noel; Fitzgibbon, Margaret American Journal of Respiratory and Critical Care Medicine, Vol. 186, Issue 1 https://doi.org/10.1164/ajrccm.186.1.110	journal	July 2012
PHYLOViZ: phylogenetic inference and data visualization for sequence based typing methods Francisco, Alexandre P.; Vaz, Cátia; Monteiro, Pedro T. BMC Bioinformatics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2105-13-87	journal	May 2012
A Bayesian approach to study the risk variables for tuberculosis occurrence in domestic and wild ungulates in South Central Spain Rodríguez-Prieto, Víctor; Martínez-López, Beatriz; Barasona, José BMC Veterinary Research, Vol. 8, Issue 1 https://doi.org/10.1186/1746-6148-8-148	journal	January 2012
Risk factors for bovine Tuberculosis at the national level in Great Britain Bessell, Paul R.; Orton, Richard; White, Piran C. L. BMC Veterinary Research, Vol. 8, Issue 1 https://doi.org/10.1186/1746-6148-8-51	journal	January 2012
Using whole genome sequencing to investigate transmission in a multi-host system: bovine tuberculosis in New Zealand Crispell, Joseph; Zadoks, Ruth N.; Harris, Simon R. BMC Genomics, Vol. 18, Issue 1 https://doi.org/10.1186/s12864-017-3569-x	journal	February 2017
Evolutionary analysis of Mycobacterium bovis genotypes across Africa suggests co-evolution with livestock and humans Inlamea, Osvaldo Frederico; Soares, Pedro; Ikuta, Cassia Yumi PLOS Neglected Tropical Diseases, Vol. 14, Issue 3 https://doi.org/10.1371/journal.pntd.0008081	journal	March 2020
Identification of drug resistance mutations among Mycobacterium bovis lineages in the Americas Vázquez-Chacón, Carlos Arturo; Rodríguez-Gaxiola, Felipe de Jesús; López-Carrera, Cruz Fernando PLOS Neglected Tropical Diseases, Vol. 15, Issue 2 https://doi.org/10.1371/journal.pntd.0009145	journal	February 2021
Epidemiological Investigation of Bovine Tuberculosis Herd Breakdowns in Spain 2009/2011 Guta, Sintayehu; Casal, Jordi; Napp, Sebastian PLoS ONE, Vol. 9, Issue 8 https://doi.org/10.1371/journal.pone.0104383	journal	August 2014
Genetic Evolution of Mycobacterium bovis Causing Tuberculosis in Livestock and Wildlife in France since 1978 Hauer, Amandine; De Cruz, Krystel; Cochard, Thierry PLOS ONE, Vol. 10, Issue 2 https://doi.org/10.1371/journal.pone.0117103	journal	February 2015
Molecular Relationship between Strains of M. bovis from Mexico and Those from Countries with Free Trade of Cattle with Mexico Milian-Suazo, Feliciano; Garcia-Casanova, Leticia; Robbe-Austerman, Suelee PLOS ONE, Vol. 11, Issue 5 https://doi.org/10.1371/journal.pone.0155207	journal	May 2016
Whole Genome Sequencing Reveals Local Transmission Patterns of Mycobacterium bovis in Sympatric Cattle and Badger Populations Biek, Roman; O'Hare, Anthony; Wright, David PLoS Pathogens, Vol. 8, Issue 11 https://doi.org/10.1371/journal.ppat.1003008	journal	November 2012
Use of genomics to track bovine tuberculosis transmission Kao, R. R.; Price-Carter, M.; Robbe-Austerman, S. Revue Scientifique et Technique de l'OIE, Vol. 35, Issue 1 https://doi.org/10.20506/rst.35.1.2430	journal	April 2016
Social Associations and Dominance of Individuals in Small Herds of Cattle Harris, Norman R.; Johnson, Douglas E.; McDougald, Neil K. Rangeland Ecology & Management, Vol. 60, Issue 4 https://doi.org/10.2111/1551-5028(2007)60[339:SAADOI]2.0.CO;2	journal	July 2007
Polyresistant Mycobacterium bovis Infection in Human and Sympatric Sheep, Spain, 2017–2018 de Val, Bernat Pérez; Romero, Beatriz; Tórtola, María Teresa Emerging Infectious Diseases, Vol. 27, Issue 4 https://doi.org/10.3201/eid2704.204467	journal	April 2021
Accurate Phylogenetic Relationships Among Mycobacterium bovis Strains Circulating in France Based on Whole Genome Sequencing and Single Nucleotide Polymorphism Analysis Hauer, Amandine; Michelet, Lorraine; Cochard, Thierry Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.00955	journal	May 2019
Global Distribution and Evolution of Mycobacterium bovis Lineages Zimpel, Cristina Kraemer; Patané, José Salvatore L.; Guedes, Aureliano Coelho Proença Frontiers in Microbiology, Vol. 11 https://doi.org/10.3389/fmicb.2020.00843	journal	May 2020
Farmer and Veterinarian Attitudes towards the Bovine Tuberculosis Eradication Programme in Spain: What Is Going on in the Field? Ciaravino, Giovanna; Ibarra, Patricia; Casal, Ester Frontiers in Veterinary Science, Vol. 4 https://doi.org/10.3389/fvets.2017.00202	journal	November 2017
Bovine Tuberculosis in Britain and Ireland – A Perfect Storm? the Confluence of Potential Ecological and Epidemiological Impediments to Controlling a Chronic Infectious Disease Allen, A. R.; Skuce, R. A.; Byrne, A. W. Frontiers in Veterinary Science, Vol. 5 https://doi.org/10.3389/fvets.2018.00109	journal	June 2018
Whole Genome Sequencing of Mycobacterium bovis Isolated From Livestock in the United States, 1989–2018 Orloski, Kathy; Robbe-Austerman, Suelee; Stuber, Tod Frontiers in Veterinary Science, Vol. 5 https://doi.org/10.3389/fvets.2018.00253	journal	October 2018
Whole Genome Sequencing for Determining the Source of Mycobacterium bovis Infections in Livestock Herds and Wildlife in New Zealand Price-Carter, Marian; Brauning, Rudiger; de Lisle, Geoffrey W. Frontiers in Veterinary Science, Vol. 5 https://doi.org/10.3389/fvets.2018.00272	journal	October 2018
Evaluation of Risk Factors Associated With Herds With an Increased Duration of Bovine Tuberculosis Breakdowns in Castilla y Leon, Spain (2010–2017) Pozo, Pilar; Romero, Beatriz; Bezos, Javier Frontiers in Veterinary Science, Vol. 7 https://doi.org/10.3389/fvets.2020.545328	journal	September 2020
Virulence factors of the Mycobacterium tuberculosis complex Forrellad, Marina A.; Klepp, Laura I.; Gioffré, Andrea Virulence, Vol. 4, Issue 1 https://doi.org/10.4161/viru.22329	journal	January 2013
Decay of Mycobacterium bovis in whole milk submitted to pasteurization parameters Ribeiro, Leandro; Narciso, Maurício Roberto Tosti; Felipe, Tatiane Hoshida Semina: Ciências Agrárias, Vol. 37, Issue 5Supl2 https://doi.org/10.5433/1679-0359.2016v37n5Supl2p3727	journal	November 2016

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59 BASIC BIOLOGICAL SCIENCES
Catalonia
Microbiology
Mycobacterium bovis
SNP
bovine tuberculosis
northeastern Spain
whole-genome sequencing

Title: Whole-Genome SNP Analysis Identifies Putative Mycobacterium bovis Transmission Clusters in Livestock and Wildlife in Catalonia, Spain

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