NLR diversity and candidate fusiform rust resistance genes in loblolly pine
Abstract
Resistance to fusiform rust disease in loblolly pine (Pinus taeda) is a classic gene-for-gene system. Early resistance gene mapping in the P. taeda family 10-5 identified RAPD markers for a major fusiform rust resistance gene, Fr1. More recently, single nucleotide polymorphism (SNP) markers associated with resistance were mapped to a full-length gene model in the loblolly pine genome encoding for a nucleotide-binding site leucine-rich repeat (NLR) protein. NLR genes are one of the most abundant gene families in plant genomes and are involved in effector-triggered immunity. Inter- and intraspecies studies of NLR gene diversity and expression have resulted in improved disease resistance. To characterize NLR gene diversity and discover potential resistance genes, we assembled de novo transcriptomes from 92 loblolly genotypes from across the natural range of the species. In these transcriptomes, we identified novel NLR transcripts that are not present in the loblolly pine reference genome and found significant geographic diversity of NLR genes providing evidence of gene family evolution. We designed capture probes for these NLRs to identify and map SNPs that stably cosegregate with resistance to the SC20-21 isolate of Cronartium quercuum f.sp. fusiforme (Cqf) in half-sib progeny of the 10-5 family. We identified 10 SNPs andmore »
- Authors:
-
[5];
- Univ. of Florida, Gainesville, FL (United States)
- Univ. of Florida, Gainesville, FL (United States); USDA Forest Service, Saucier, MS (United States). Southern Institute of Forest Genetics
- Univ. of Kentucky, Lexington, KY (United States)
- Rapid Genomics, Gainesville, FL (United States)
- Univ. of Connecticut, Storrs, CT (United States)
- USDA Forest Service, Saucier, MS (United States). Southern Institute of Forest Genetics; USDA Forest Service, Lexington, KY (United States). Southern Research Station, Forest Health Research and Education Center
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); USDA
- OSTI Identifier:
- 1904366
- Grant/Contract Number:
- SC0019099; 2017-67012-26097
- Resource Type:
- Accepted Manuscript
- Journal Name:
- G3
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 2; Journal ID: ISSN 2160-1836
- Publisher:
- Genetics Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; NLR; RNAseq; fusiform rust; resistance genes; Pinus taeda; Fr genes; sequence-capture; pan-NLRome
Citation Formats
Ence, Daniel, Smith, Katherine E., Fan, Shenghua, Gomide Neves, Leandro, Paul, Robin, Wegrzyn, Jill, Peter, Gary F., Kirst, Matias, Brawner, Jeremy, Nelson, C. Dana, and Davis, John M. NLR diversity and candidate fusiform rust resistance genes in loblolly pine. United States: N. p., 2021.
Web. doi:10.1093/g3journal/jkab421.
Ence, Daniel, Smith, Katherine E., Fan, Shenghua, Gomide Neves, Leandro, Paul, Robin, Wegrzyn, Jill, Peter, Gary F., Kirst, Matias, Brawner, Jeremy, Nelson, C. Dana, & Davis, John M. NLR diversity and candidate fusiform rust resistance genes in loblolly pine. United States. https://doi.org/10.1093/g3journal/jkab421
Ence, Daniel, Smith, Katherine E., Fan, Shenghua, Gomide Neves, Leandro, Paul, Robin, Wegrzyn, Jill, Peter, Gary F., Kirst, Matias, Brawner, Jeremy, Nelson, C. Dana, and Davis, John M. Mon .
"NLR diversity and candidate fusiform rust resistance genes in loblolly pine". United States. https://doi.org/10.1093/g3journal/jkab421. https://www.osti.gov/servlets/purl/1904366.
@article{osti_1904366,
title = {NLR diversity and candidate fusiform rust resistance genes in loblolly pine},
author = {Ence, Daniel and Smith, Katherine E. and Fan, Shenghua and Gomide Neves, Leandro and Paul, Robin and Wegrzyn, Jill and Peter, Gary F. and Kirst, Matias and Brawner, Jeremy and Nelson, C. Dana and Davis, John M.},
abstractNote = {Resistance to fusiform rust disease in loblolly pine (Pinus taeda) is a classic gene-for-gene system. Early resistance gene mapping in the P. taeda family 10-5 identified RAPD markers for a major fusiform rust resistance gene, Fr1. More recently, single nucleotide polymorphism (SNP) markers associated with resistance were mapped to a full-length gene model in the loblolly pine genome encoding for a nucleotide-binding site leucine-rich repeat (NLR) protein. NLR genes are one of the most abundant gene families in plant genomes and are involved in effector-triggered immunity. Inter- and intraspecies studies of NLR gene diversity and expression have resulted in improved disease resistance. To characterize NLR gene diversity and discover potential resistance genes, we assembled de novo transcriptomes from 92 loblolly genotypes from across the natural range of the species. In these transcriptomes, we identified novel NLR transcripts that are not present in the loblolly pine reference genome and found significant geographic diversity of NLR genes providing evidence of gene family evolution. We designed capture probes for these NLRs to identify and map SNPs that stably cosegregate with resistance to the SC20-21 isolate of Cronartium quercuum f.sp. fusiforme (Cqf) in half-sib progeny of the 10-5 family. We identified 10 SNPs and 2 quantitative trait loci associated with resistance to SC20-21 Cqf. The geographic diversity of NLR genes provides evidence of NLR gene family evolution in loblolly pine. The SNPs associated with rust resistance provide a resource to enhance breeding and deployment of resistant pine seedlings.},
doi = {10.1093/g3journal/jkab421},
journal = {G3},
number = 2,
volume = 12,
place = {United States},
year = {Mon Dec 13 00:00:00 EST 2021},
month = {Mon Dec 13 00:00:00 EST 2021}
}
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Works referenced in this record:
qqman: an R package for visualizing GWAS results using Q-Q and manhattan plots
journal, May 2018
- D. Turner, Stephen
- Journal of Open Source Software, Vol. 3, Issue 25
UpSet: Visualization of Intersecting Sets
journal, December 2014
- Lex, Alexander; Gehlenborg, Nils; Strobelt, Hendrik
- IEEE Transactions on Visualization and Computer Graphics, Vol. 20, Issue 12
A Consensus Genetic Map forPinus taedaandPinus elliottiiand Extent of Linkage Disequilibrium in Two Genotype-Phenotype Discovery Populations ofPinus taeda
journal, August 2015
- Westbrook, Jared W.; Chhatre, Vikram E.; Wu, Le-Shin
- G3 Genes|Genomes|Genetics, Vol. 5, Issue 8
Identification and localisation of the NB-LRR gene family within the potato genome
journal, January 2012
- Jupe, Florian; Pritchard, Leighton; Etherington, Graham J.
- BMC Genomics, Vol. 13, Issue 1
Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences
journal, May 2006
- Li, W.; Godzik, A.
- Bioinformatics, Vol. 22, Issue 13
From FastQ Data to High‐Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline
journal, October 2013
- Auwera, Geraldine A.; Carneiro, Mauricio O.; Hartl, Christopher
- Current Protocols in Bioinformatics, Vol. 43, Issue 1
Dominant integration locus drives continuous diversification of plant immune receptors with exogenous domain fusions
journal, February 2018
- Bailey, Paul C.; Schudoma, Christian; Jackson, William
- Genome Biology, Vol. 19, Issue 1
20 years of the SMART protein domain annotation resource
journal, October 2017
- Letunic, Ivica; Bork, Peer
- Nucleic Acids Research, Vol. 46, Issue D1
Association Genetics in Pinus taeda L. I. Wood Property Traits
journal, November 2006
- González-Martínez, Santiago C.; Wheeler, Nicholas C.; Ersoz, Elhan
- Genetics, Vol. 175, Issue 1
Genetic Interaction of the Fusiform Rust Fungus with Resistance Gene Fr1 in Loblolly Pine
journal, April 2005
- Kubisiak, Thomas L.; Amerson, Henry V.; Nelson, C. Dana
- Phytopathology®, Vol. 95, Issue 4
De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis
journal, July 2013
- Haas, Brian J.; Papanicolaou, Alexie; Yassour, Moran
- Nature Protocols, Vol. 8, Issue 8
Fast and accurate short read alignment with Burrows-Wheeler transform
journal, May 2009
- Li, H.; Durbin, R.
- Bioinformatics, Vol. 25, Issue 14
Pronounced Intraspecific Haplotype Divergence at the RPP5 Complex Disease Resistance Locus of Arabidopsis
journal, November 1999
- Noël, Laurent; Moores, Tracey L.; van der Biezen, Erik A.
- The Plant Cell, Vol. 11, Issue 11
InterPro in 2019: improving coverage, classification and access to protein sequence annotations
journal, November 2018
- Mitchell, Alex L.; Attwood, Teresa K.; Babbitt, Patricia C.
- Nucleic Acids Research, Vol. 47, Issue D1
Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies
journal, January 2014
- Neale, David B.; Wegrzyn, Jill L.; Stevens, Kristian A.
- Genome Biology, Vol. 15, Issue 3
Sequence of the Sugar Pine Megagenome
journal, October 2016
- Stevens, Kristian A.; Wegrzyn, Jill L.; Zimin, Aleksey
- Genetics, Vol. 204, Issue 4
Deriving the consequences of genomic variants with the Ensembl API and SNP Effect Predictor
journal, June 2010
- McLaren, William; Pritchard, Bethan; Rios, Daniel
- Bioinformatics, Vol. 26, Issue 16
Chromosome level assembly of the hybrid Trypanosoma cruzi genome
journal, June 2009
- Weatherly, D. Brent; Boehlke, Courtney; Tarleton, Rick L.
- BMC Genomics, Vol. 10, Issue 1
Clusters of Resistance Genes in Plants Evolve by Divergent Selection and a Birth-and-Death Process
journal, November 1998
- Michelmore, Richard W.; Meyers, Blake C.
- Genome Research, Vol. 8, Issue 11
DNA Sequence Variation and Selection of Tag Single-Nucleotide Polymorphisms at Candidate Genes for Drought-Stress Response in Pinus taeda L.
journal, December 2005
- González-Martínez, Santiago C.; Ersoz, Elhan; Brown, Garth R.
- Genetics, Vol. 172, Issue 3
Structure, function and evolution of plant disease resistance genes
journal, August 2000
- Ellis, Jeff; Dodds, Peter; Pryor, Tony
- Current Opinion in Plant Biology, Vol. 3, Issue 4
A Reference Genome Sequence for Giant Sequoia
journal, November 2020
- Scott, Alison D.; Zimin, Aleksey V.; Puiu, Daniela
- G3 Genes|Genomes|Genetics, Vol. 10, Issue 11
The large repertoire of conifer NLR resistance genes includes drought responsive and highly diversified RNLs
journal, August 2019
- Van Ghelder, Cyril; Parent, Geneviève J.; Rigault, Philippe
- Scientific Reports, Vol. 9, Issue 1
Unified representation of genetic variants
journal, February 2015
- Tan, Adrian; Abecasis, Gonçalo R.; Kang, Hyun Min
- Bioinformatics, Vol. 31, Issue 13
Plant NLR diversity: the known unknowns of pan-NLRomes
journal, February 2021
- Barragan, A. Cristina; Weigel, Detlef
- The Plant Cell, Vol. 33, Issue 4
Multiple Genetic Processes Result in Heterogeneous Rates of Evolution within the Major Cluster Disease Resistance Genes in Lettuce[W]
journal, November 2004
- Kuang, Hanhui; Woo, Sung-Sick; Meyers, Blake C.
- The Plant Cell, Vol. 16, Issue 11
Whole-exome targeted sequencing of the uncharacterized pine genome
journal, May 2013
- Neves, Leandro G.; Davis, John M.; Barbazuk, William B.
- The Plant Journal, Vol. 75, Issue 1
RPS2 of Arabidopsis thaliana: a leucine-rich repeat class of plant disease resistance genes
journal, September 1994
- Bent, A.; Kunkel, B.; Dahlbeck, D.
- Science, Vol. 265, Issue 5180
CD-HIT: accelerated for clustering the next-generation sequencing data
journal, October 2012
- Fu, Limin; Niu, Beifang; Zhu, Zhengwei
- Bioinformatics, Vol. 28, Issue 23
Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire
journal, July 2017
- Giolai, Michael; Paajanen, Pirita; Verweij, Walter
- BMC Genomics, Vol. 18, Issue 1
Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing
journal, April 2016
- Witek, Kamil; Jupe, Florian; Witek, Agnieszka I.
- Nature Biotechnology, Vol. 34, Issue 6
Ridge Regression and Other Kernels for Genomic Selection with R Package rrBLUP
journal, January 2011
- Endelman, Jeffrey B.
- The Plant Genome Journal, Vol. 4, Issue 3
An Economic Evaluation of Fusiform Rust Protection Research
journal, May 2000
- Cubbage, Frederick W.; Pye, John M.; Holmes, Thomas P.
- Southern Journal of Applied Forestry, Vol. 24, Issue 2
Current Status of the Gene-For-Gene Concept
journal, September 1971
- Flor, H. H.
- Annual Review of Phytopathology, Vol. 9, Issue 1
Resistance gene enrichment sequencing (RenSeq) enables reannotation of the NB-LRR gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations
journal, October 2013
- Jupe, Florian; Witek, Kamil; Verweij, Walter
- The Plant Journal, Vol. 76, Issue 3
Detection of a major gene for resistance to fusiform rust disease in loblolly pine by genomic mapping.
journal, April 1996
- Wilcox, P. L.; Amerson, H. V.; Kuhlman, E. G.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 9
The plant immune system
journal, November 2006
- Jones, Jonathan D. G.; Dangl, Jeffery L.
- Nature, Vol. 444, Issue 7117
The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats
journal, September 1994
- Mindrinos, Michael; Katagiri, Fumiaki; Yu, Guo-Liang
- Cell, Vol. 78, Issue 6
Identification of Nine Pathotype-Specific Genes Conferring Resistance to Fusiform Rust in Loblolly Pine (Pinus taeda L.)
journal, August 2015
- Amerson, Henry; Nelson, C.; Kubisiak, Thomas
- Forests, Vol. 6, Issue 12
From Guard to Decoy: A New Model for Perception of Plant Pathogen Effectors
journal, August 2008
- van der Hoorn, Renier A. L.; Kamoun, Sophien
- The Plant Cell, Vol. 20, Issue 8
BED-domain-containing immune receptors confer diverse resistance spectra to yellow rust
journal, August 2018
- Marchal, Clemence; Zhang, Jianping; Zhang, Peng
- Nature Plants, Vol. 4, Issue 9
InterProScan 5: genome-scale protein function classification
journal, January 2014
- Jones, P.; Binns, D.; Chang, H. -Y.
- Bioinformatics, Vol. 30, Issue 9
Variation in virulence among single-aeciospore isolates from single-gall isolates of Cronartiumquercuum f.sp. fusiforme
journal, January 1993
- Kuhlman, E. G.; Matthews, F. R.
- Canadian Journal of Forest Research, Vol. 23, Issue 1
Unravelling and managing fusiform rust disease: a model approach for coevolved forest tree pathosystems
journal, February 2010
- Nelson, C. D.; Kubisiak, T. L.; Amerson, H. V.
- Forest Pathology, Vol. 40, Issue 1
A novel conserved mechanism for plant NLR protein pairs: the “integrated decoy†hypothesis
journal, November 2014
- Cesari, Stella; Bernoux, Maud; Moncuquet, Philippe
- Frontiers in Plant Science, Vol. 5
Genome-Wide Analysis of NBS-LRR–Encoding Genes in Arabidopsis
journal, March 2003
- Meyers, Blake C.; Kozik, Alexander; Griego, Alyssa
- The Plant Cell, Vol. 15, Issue 4
Population dynamics of Pinus taeda L. based on nuclear microsatellites
journal, June 2002
- Al-Rabab’ah, M. A.; Williams, C. G.
- Forest Ecology and Management, Vol. 163, Issue 1-3
Fifty-two polymorphic microsatellite loci in the rust fungus, Cronartium quercuum f.sp. fusiforme
journal, November 2007
- Burdine, C. S.; Kubisiak, T. L.; Johnson, G. N.
- Molecular Ecology Notes, Vol. 7, Issue 6
Patterns of Population Structure and Environmental Associations to Aridity Across the Range of Loblolly Pine ( Pinus taeda L., Pinaceae)
journal, May 2010
- Eckert, Andrew J.; van Heerwaarden, Joost; Wegrzyn, Jill L.
- Genetics, Vol. 185, Issue 3
The Pfam protein families database in 2019
journal, October 2018
- El-Gebali, Sara; Mistry, Jaina; Bateman, Alex
- Nucleic Acids Research, Vol. 47, Issue D1
The Pfam protein families database: towards a more sustainable future
journal, December 2015
- Finn, Robert D.; Coggill, Penelope; Eberhardt, Ruth Y.
- Nucleic Acids Research, Vol. 44, Issue D1
Subsets of NLR genes show differential signatures of adaptation during colonization of new habitats
journal, June 2019
- Stam, Remco; Silva‐Arias, Gustavo A.; Tellier, Aurelien
- New Phytologist, Vol. 224, Issue 1
MEME SUITE: tools for motif discovery and searching
journal, May 2009
- Bailey, T. L.; Boden, M.; Buske, F. A.
- Nucleic Acids Research, Vol. 37, Issue Web Server
Chromosome-level assemblies of multiple Arabidopsis genomes reveal hotspots of rearrangements with altered evolutionary dynamics
journal, February 2020
- Jiao, Wen-Biao; Schneeberger, Korbinian
- Nature Communications, Vol. 11, Issue 1
Pm21 from Haynaldia villosa Encodes a CC-NBS-LRR Protein Conferring Powdery Mildew Resistance in Wheat
journal, June 2018
- Xing, Liping; Hu, Ping; Liu, Jiaqian
- Molecular Plant, Vol. 11, Issue 6
A High-Density Gene Map of Loblolly Pine ( Pinus taeda L.) Based on Exome Sequence Capture Genotyping
journal, November 2013
- Neves, Leandro Gomide; Davis, John M.; Barbazuk, William B.
- G3: Genes|Genomes|Genetics, Vol. 4, Issue 1
Inoculum Density and Expression of Major Gene Resistance to Fusiform Rust Disease in Loblolly Pine
journal, June 1997
- Kuhlman, E. G.; Amerson, H. V.; Jordan, A. P.
- Plant Disease, Vol. 81, Issue 6
Resistance Responses in Half-Sib Loblolly Pine Progenies after Inoculation withCronartium quercuumf. sp.fusiforme
journal, January 1988
- Kuhlman, E. G.
- Phytopathology, Vol. 78, Issue 4
A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana
journal, August 2019
- Van de Weyer, Anna-Lena; Monteiro, Freddy; Furzer, Oliver J.
- Cell, Vol. 178, Issue 5
Mapping Fusiform Rust Resistance Genes within a Complex Mating Design of Loblolly Pine
journal, February 2014
- Quesada, Tania; Resende Jr., Marcio; Muñoz, Patricio
- Forests, Vol. 5, Issue 2
Genetic diversity within and among populations of shortleaf pine (Pinus echinata Mill.) and loblolly pine (Pinus taeda L.)
journal, June 2008
- Xu, Shiqin; Tauer, C. G.; Nelson, C. Dana
- Tree Genetics & Genomes, Vol. 4, Issue 4
A genomic map enriched for markers linked to Avr1 in Cronartium quercuum f.sp. fusiforme
journal, March 2011
- Kubisiak, Thomas L.; Anderson, Claire L.; Amerson, Henry V.
- Fungal Genetics and Biology, Vol. 48, Issue 3
The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data
journal, July 2010
- McKenna, A.; Hanna, M.; Banks, E.
- Genome Research, Vol. 20, Issue 9
Narrative History of the Resistance Screening Center: It’s Origins, Leadership and Partial List of Public Benefits and Scientific Contributions
journal, August 2013
- Cowling, Ellis; Young, Carol
- Forests, Vol. 4, Issue 3
Unique Features of the Loblolly Pine ( Pinus taeda L.) Megagenome Revealed Through Sequence Annotation
journal, March 2014
- Wegrzyn, Jill L.; Liechty, John D.; Stevens, Kristian A.
- Genetics, Vol. 196, Issue 3
Chromosome-Scale Assembly of the Bread Wheat Genome Reveals Thousands of Additional Gene Copies
journal, October 2020
- Alonge, Michael; Shumate, Alaina; Puiu, Daniela
- Genetics, Vol. 216, Issue 2
Differential Expression of Soybean Cysteine Proteinase Inhibitor Genes during Development and in Response to Wounding and Methyl Jasmonate
journal, November 1996
- Botella, M. A.; Xu, Y.; Prabha, T. N.
- Plant Physiology, Vol. 112, Issue 3
The variant call format and VCFtools
journal, June 2011
- Danecek, P.; Auton, A.; Abecasis, G.
- Bioinformatics, Vol. 27, Issue 15
Interactions of Fr genes and mixed-pathogen inocula in the loblolly pine-fusiform rust pathosystem
journal, August 2011
- Isik, Fikret; Amerson, Henry V.; Whetten, Ross W.
- Tree Genetics & Genomes, Vol. 8, Issue 1
Major QTL confer race-nonspecific resistance in the co-evolved Cronartium quercuum f. sp. fusiforme–Pinus taeda pathosystem
journal, June 2021
- Lauer, Edwin; Isik, Fikret
- Heredity, Vol. 127, Issue 3
Resistance of Pinus taeda families under artificial inoculations with diverse fusiform rust pathogen populations and comparison with field trials
journal, October 2008
- Isik, Fikret; Amerson, Henry V.; Whetten, Ross W.
- Canadian Journal of Forest Research, Vol. 38, Issue 10
The Ensembl Variant Effect Predictor
journal, June 2016
- McLaren, William; Gil, Laurent; Hunt, Sarah E.
- Genome Biology, Vol. 17, Issue 1
The Pinus taeda genome is characterized by diverse and highly diverged repetitive sequences
journal, January 2010
- Kovach, Allen; Wegrzyn, Jill L.; Parra, Genis
- BMC Genomics, Vol. 11, Issue 1
Prescreening Slash Pine and Cronartium Pedigrees for Evaluation of Complementary Gene Action in Fusiform Rust Disease
journal, April 1999
- Stelzer, H. E.; Doudrick, R. L.; Kubisiak, T. L.
- Plant Disease, Vol. 83, Issue 4
SMART: recent updates, new developments and status in 2015
journal, October 2014
- Letunic, Ivica; Doerks, Tobias; Bork, Peer
- Nucleic Acids Research, Vol. 43, Issue D1
The Sequence Alignment/Map format and SAMtools
journal, June 2009
- Li, H.; Handsaker, B.; Wysoker, A.
- Bioinformatics, Vol. 25, Issue 16
The product of the tobacco mosaic virus resistance gene N: Similarity to toll and the interleukin-1 receptor
journal, September 1994
- Whitham, Steve; Dinesh-Kumar, S. P.; Choi, Doil
- Cell, Vol. 78, Issue 6
An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations
journal, April 2017
- Clavijo, Bernardo J.; Venturini, Luca; Schudoma, Christian
- Genome Research, Vol. 27, Issue 5
Evolution of Plant NLRs: From Natural History to Precise Modifications
journal, April 2020
- Tamborski, Janina; Krasileva, Ksenia V.
- Annual Review of Plant Biology, Vol. 71, Issue 1
Improved white spruce (Picea glauca) genome assemblies and annotation of large gene families of conifer terpenoid and phenolic defense metabolism
journal, June 2015
- Warren, René L.; Keeling, Christopher I.; Yuen, Macaire Man Saint
- The Plant Journal, Vol. 83, Issue 2