Re-evaluating the Systematics of Dendrolycopodium Using Restriction-Site Associated DNA-Sequencing
- Boyce Thompson Institute, Ithaca, NY (United States); Cornell Univ., Ithaca, NY (United States); OSTI
- Boyce Thompson Institute, Ithaca, NY (United States)
- HudsonAlpha Institute for Biotechnology, Huntsville, AL
- HudsonAlpha Institute for Biotechnology, Huntsville, AL; USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
- Boyce Thompson Institute, Ithaca, NY (United States); Cornell Univ., Ithaca, NY (United States)
The genus Dendrolycopodium (Lycopodiaceae) includes four to five species across North America and East Asia. Species identification in Dendrolycopodium is difficult due to limited or inconsistent characters. In addition, plants with intermediate morphologies regularly occur, potentially indicative of interspecific hybridization. To determine the species relationships in Dendrolycopodium and investigate the existence of hybrids, we generated a draft genome assembly for D. obscurum and carried out double-digest restriction-site associated DNA sequencing (RADSeq) on 86 Dendrolycopodium specimens. Our sampling includes all the described species and 11 individuals with intermediate morphology. We find that the genus can be divided into four clades that largely correspond to the described taxa, as well as evidence of interspecific hybridization. Within these clades, our STRUCTURE analysis suggests that there are multiple finer subgroups, with evidence of hybridization and introgression between these subgroups. Given the limited availability of specimens collected from Asia, the status of the various Asian species remains uncertain and will require further study. In summary, our study confirms several hybrid relationships in Dendrolycopodium and provides a clear phylogenetic framework for future taxonomic revision.
- Research Organization:
- USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- AC02-05CH11231
- OSTI ID:
- 1904075
- Journal Information:
- Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 13; ISSN 1664-462X
- Publisher:
- Frontiers Research FoundationCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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