Genome-wide transcriptional regulation in Saccharomyces cerevisiae in response to carbon dioxide

Tan, Lin-Rui; Liu, Jing-Jing; Deewan, Anshu; Lee, Jae Won; Xia, Peng-Fei; Rao, Christopher V.; Jin, Yong-Su; Wang, Shu-Guang

doi:10.1093/femsyr/foac032

Title: Genome-wide transcriptional regulation in Saccharomyces cerevisiae in response to carbon dioxide

Journal Article · Tue May 31 00:00:00 UTC 2022 · FEMS Yeast Research

DOI: https://doi.org/10.1093/femsyr/foac032 · OSTI ID:1902743

Tan, Lin-Rui ^[1]; Liu, Jing-Jing ^[2]; Deewan, Anshu ^[2]; Lee, Jae Won ^[2]; Xia, Peng-Fei ^[3]; Rao, Christopher V. ^[2];

^[2]; Wang, Shu-Guang ^[3]

Shandong Univ., Qingdao (China); Univ. of Illinois at Urbana-Champaign, IL (United States); University of Illinois
Univ. of Illinois at Urbana-Champaign, IL (United States)
Shandong Univ., Qingdao (China)

Sugar metabolism by Saccharomyces cerevisiae produces ample amounts of CO₂ under both aerobic and anaerobic conditions. High solubility of CO₂ in fermentation media, contributing to enjoyable sensory properties of sparkling wine and beers by S. cerevisiae, might affect yeast metabolism. To elucidate the overlooked effects of CO₂ on yeast metabolism, we examined glucose fermentation by S. cerevisiae under CO₂ as compared to N₂ and O₂ limited conditions. While both CO₂ and N₂ conditions are considered anaerobic, less glycerol and acetate but more ethanol were produced under CO₂ condition. Transcriptomic analysis revealed that significantly decreased mRNA levels of GPP1 coding for glycerol-3-phosphate phosphatase in glycerol synthesis explained the reduced glycerol production under CO₂ condition. Besides, transcriptional regulations in signal transduction, carbohydrate synthesis, heme synthesis, membrane and cell wall metabolism, and respiration were detected in response to CO₂. Interestingly, signal transduction was uniquely regulated under CO₂ condition, where upregulated genes (STE3, MSB2, WSC3, STE12, and TEC1) in the signal sensors and transcriptional factors suggested that MAPK signaling pathway plays a critical role in CO₂ sensing and CO₂-induced metabolisms in yeast. Furthermore, our study identifies CO₂ as an external stimulus for modulating metabolic activities in yeast and a transcriptional effector for diverse applications.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420

OSTI ID:: 1902743

Journal Information:: FEMS Yeast Research, Journal Name: FEMS Yeast Research Journal Issue: 1 Vol. 22; ISSN 1567-1364

Publisher:: Federation of European Microbiological Societies - Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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