Photobiocatalysis for Abiological Transformations
Abstract
Harnessing biocatalysts for novel abiological transformations is a longstanding goal of synthetic chemistry. Combining the merits of biocatalysis and photocatalysis allows for selective transformations fueled by visible light and offers many advantages including new reactivity, high enantioselectivity, greener syntheses, and high yields. Photoinduced electron or energy transfer enables synthetic methodologies that complement conventional two electron processes or offer orthogonal pathways for developing new reactions. Enzymes are well suited and can be tuned by directed evolution to exert control over radical intermediates thereby suppressing undesirable reactions and delivering high chemo- and stereoselectivities. Within the past decade, the combination of biocatalysis and photocatalysis was mainly focused on exploiting light-regenerated cofactors to increase native enzymatic activity. However, recent developments have demonstrated that the combination can unlock new-to-nature chemistry. Particularly, the discovery and application of new strategies are well poised to expand the applications of photobiocatalysis. In the past three years, our lab has been studying the combinations of photocatalysis and biocatalysis which can be applied to create new synthetic methodologies and solve challenges in synthetic organic chemistry. Our efforts have expanded the strategies for combining external photocatalysts with enzymes through the construction of a synergistic cooperative stereoconvergent reduction system consisting of photosensitized energymore »
- Authors:
-
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1902721
- Grant/Contract Number:
- SC0018420
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Accounts of Chemical Research
- Additional Journal Information:
- Journal Volume: 55; Journal Issue: 8; Journal ID: ISSN 0001-4842
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Electromagnetic radiation; Peptides and proteins; Photocatalysis; Photocatalysts; Redox reactions
Citation Formats
Harrison, Wesley, Huang, Xiaoqiang, and Zhao, Huimin. Photobiocatalysis for Abiological Transformations. United States: N. p., 2022.
Web. doi:10.1021/acs.accounts.1c00719.
Harrison, Wesley, Huang, Xiaoqiang, & Zhao, Huimin. Photobiocatalysis for Abiological Transformations. United States. https://doi.org/10.1021/acs.accounts.1c00719
Harrison, Wesley, Huang, Xiaoqiang, and Zhao, Huimin. Wed .
"Photobiocatalysis for Abiological Transformations". United States. https://doi.org/10.1021/acs.accounts.1c00719. https://www.osti.gov/servlets/purl/1902721.
@article{osti_1902721,
title = {Photobiocatalysis for Abiological Transformations},
author = {Harrison, Wesley and Huang, Xiaoqiang and Zhao, Huimin},
abstractNote = {Harnessing biocatalysts for novel abiological transformations is a longstanding goal of synthetic chemistry. Combining the merits of biocatalysis and photocatalysis allows for selective transformations fueled by visible light and offers many advantages including new reactivity, high enantioselectivity, greener syntheses, and high yields. Photoinduced electron or energy transfer enables synthetic methodologies that complement conventional two electron processes or offer orthogonal pathways for developing new reactions. Enzymes are well suited and can be tuned by directed evolution to exert control over radical intermediates thereby suppressing undesirable reactions and delivering high chemo- and stereoselectivities. Within the past decade, the combination of biocatalysis and photocatalysis was mainly focused on exploiting light-regenerated cofactors to increase native enzymatic activity. However, recent developments have demonstrated that the combination can unlock new-to-nature chemistry. Particularly, the discovery and application of new strategies are well poised to expand the applications of photobiocatalysis. In the past three years, our lab has been studying the combinations of photocatalysis and biocatalysis which can be applied to create new synthetic methodologies and solve challenges in synthetic organic chemistry. Our efforts have expanded the strategies for combining external photocatalysts with enzymes through the construction of a synergistic cooperative stereoconvergent reduction system consisting of photosensitized energy transfer and ene-reductase catalyzed alkene reduction. Additionally, our efforts have also extended the capability of cofactor-dependent photoenzymatic systems to include enantioselective bimolecular radical hydroalkylations of alkenes by irradiating electron donor-acceptor complexes comprised of enzymatic redox active cofactors and unnatural substrates. In this Account, we highlight strategies developed by our group and others for combining biocatalysis and photocatalysis with the aim of introducing nonnatural reactivity to enzymes. Presently, strategies applied to achieve this goal include the repurposing of newly discovered photoactive enzymes, the elucidation of new photoreactivity within cofactor-dependent enzymes, the combination of external photocatalysts with enzymes, and the construction of artificial photoenzymes. By demonstrating the successful application of these strategies for achieving selective new-to-nature transformations, we hope to spur interest in the expanding the scope of photobiocatalytic systems through the use and extension of these strategies and creation of new strategies. In addition, we hope to elucidate the intuition in synergizing the unique capabilities of biocatalysis and photocatalysis so that photobiocatalysis can be recognized as a potential solution to difficult challenges in synthetic organic chemistry.},
doi = {10.1021/acs.accounts.1c00719},
journal = {Accounts of Chemical Research},
number = 8,
volume = 55,
place = {United States},
year = {Wed Mar 30 00:00:00 EDT 2022},
month = {Wed Mar 30 00:00:00 EDT 2022}
}
Works referenced in this record:
Chemomimetic Biocatalysis: Exploiting the Synthetic Potential of Cofactor-Dependent Enzymes To Create New Catalysts
journal, November 2015
- Prier, Christopher K.; Arnold, Frances H.
- Journal of the American Chemical Society, Vol. 137, Issue 44
Photoenzymatic Hydrogenation of Heteroaromatic Olefins Using ‘Ene’‐Reductases with Photoredox Catalysts
journal, April 2020
- Nakano, Yuji; Black, Michael J.; Meichan, Andrew J.
- Angewandte Chemie International Edition, Vol. 59, Issue 26
Triplet Energy Transfer Photocatalysis: Unlocking the Next Level
journal, August 2020
- Strieth-Kalthoff, Felix; Glorius, Frank
- Chem, Vol. 6, Issue 8
Structure and Function of the Photosystem Supercomplexes
journal, March 2018
- Gao, Jinlan; Wang, Hao; Yuan, Qipeng
- Frontiers in Plant Science, Vol. 9
Biocatalytic Cross-Coupling of Aryl Halides with a Genetically Engineered Photosensitizer Artificial Dehalogenase
journal, January 2021
- Fu, Yu; Huang, Jian; Wu, Yuzhou
- Journal of the American Chemical Society, Vol. 143, Issue 2
Engineering Fatty Acid Photodecarboxylase to Enable Highly Selective Decarboxylation of
trans
Fatty Acids
journal, August 2021
- Li, Danyang; Han, Tao; Xue, Jiadan
- Angewandte Chemie International Edition, Vol. 60, Issue 38
Photoenzymatic Reductions Enabled by Direct Excitation of Flavin-Dependent “Ene”-Reductases
journal, December 2020
- Sandoval, Braddock A.; Clayman, Phillip D.; Oblinsky, Daniel G.
- Journal of the American Chemical Society, Vol. 143, Issue 4
Organic Photoredox Catalysis
journal, June 2016
- Romero, Nathan A.; Nicewicz, David A.
- Chemical Reviews, Vol. 116, Issue 17
Engineering new catalytic activities in enzymes
journal, January 2020
- Chen, Kai; Arnold, Frances H.
- Nature Catalysis, Vol. 3, Issue 3
Stereoconvergent Reduction of Activated Alkenes by a Nicotinamide Free Synergistic Photobiocatalytic System
journal, July 2020
- Wang, Yajie; Huang, Xiaoqiang; Hui, Jingshu
- ACS Catalysis, Vol. 10, Issue 16
An algal photoenzyme converts fatty acids to hydrocarbons
journal, August 2017
- Sorigué, Damien; Légeret, Bertrand; Cuiné, Stéphan
- Science, Vol. 357, Issue 6354
Photoenzymatic Catalysis Enables Radical‐Mediated Ketone Reduction in Ene‐Reductases
journal, June 2019
- Sandoval, Braddock A.; Kurtoic, Sarah I.; Chung, Megan M.
- Angewandte Chemie International Edition, Vol. 58, Issue 26
Visible Light Photoredox Catalysis with Transition Metal Complexes: Applications in Organic Synthesis
journal, March 2013
- Prier, Christopher K.; Rankic, Danica A.; MacMillan, David W. C.
- Chemical Reviews, Vol. 113, Issue 7
Photoenzymatic Generation of Unstabilized Alkyl Radicals: An Asymmetric Reductive Cyclization
journal, August 2020
- Clayman, Phillip D.; Hyster, Todd K.
- Journal of the American Chemical Society, Vol. 142, Issue 37
Synthetic Methods Driven by the Photoactivity of Electron Donor–Acceptor Complexes
journal, March 2020
- Crisenza, Giacomo E. M.; Mazzarella, Daniele; Melchiorre, Paolo
- Journal of the American Chemical Society, Vol. 142, Issue 12
Rational Design of a Miniature Photocatalytic CO2-Reducing Enzyme
journal, April 2021
- Kang, Fuying; Yu, Lu; Xia, Yan
- ACS Catalysis, Vol. 11, Issue 9
Photoenzymatic Synthesis of α-Tertiary Amines by Engineered Flavin-Dependent “Ene”-Reductases
journal, November 2021
- Gao, Xin; Turek-Herman, Joshua R.; Choi, Young Joo
- Journal of the American Chemical Society, Vol. 143, Issue 47
Catalytic promiscuity enabled by photoredox catalysis in nicotinamide-dependent oxidoreductases
journal, June 2018
- Biegasiewicz, Kyle F.; Cooper, Simon J.; Emmanuel, Megan A.
- Nature Chemistry, Vol. 10, Issue 7
Preparation, Characterization, and Oxygenase Activity of a Photocatalytic Artificial Enzyme
journal, July 2015
- Gu, Yifan; Ellis-Guardiola, Ken; Srivastava, Poonam
- ChemBioChem, Vol. 16, Issue 13
Asymmetric catalysis activated by visible light
journal, January 2015
- Meggers, Eric
- Chemical Communications, Vol. 51, Issue 16
Engineering a Non‐Natural Photoenzyme for Improved Photon Efficiency**
journal, December 2021
- Nicholls, Bryce T.; Oblinsky, Daniel G.; Kurtoic, Sarah I.
- Angewandte Chemie International Edition, Vol. 61, Issue 2
Mechanism and dynamics of fatty acid photodecarboxylase
journal, April 2021
- Sorigué, D.; Hadjidemetriou, K.; Blangy, S.
- Science, Vol. 372, Issue 6538
Structure and Function of DNA Photolyase and Cryptochrome Blue-Light Photoreceptors
journal, June 2003
- Sancar, Aziz
- Chemical Reviews, Vol. 103, Issue 6
Bioinspired NADH Regeneration Based on Conjugated Photocatalytic Systems
journal, August 2020
- Zhang, Yuanyuan; Zhao, Yingjie; Li, Run
- Solar RRL, Vol. 5, Issue 2
Review of NAD(P)H-dependent oxidoreductases: Properties, engineering and application
journal, February 2018
- Sellés Vidal, Lara; Kelly, Ciarán L.; Mordaka, Paweł M.
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1866, Issue 2
Photoredox Catalysis in Organic Chemistry
journal, June 2016
- Shaw, Megan H.; Twilton, Jack; MacMillan, David W. C.
- The Journal of Organic Chemistry, Vol. 81, Issue 16
Light-Dependent Protochlorophyllide Oxidoreductase: Phylogeny, Regulation, and Catalytic Properties
journal, August 2015
- Gabruk, Michal; Mysliwa-Kurdziel, Beata
- Biochemistry, Vol. 54, Issue 34
Cooperative asymmetric reactions combining photocatalysis and enzymatic catalysis
journal, August 2018
- Litman, Zachary C.; Wang, Yajie; Zhao, Huimin
- Nature, Vol. 560, Issue 7718
Enzyme-photo-coupled catalytic systems
journal, January 2021
- Zhang, Shaohua; Liu, Shusong; Sun, Yiying
- Chemical Society Reviews, Vol. 50, Issue 24
Visible-Light Photocatalysis: Does It Make a Difference in Organic Synthesis?
journal, June 2018
- Marzo, Leyre; Pagire, Santosh K.; Reiser, Oliver
- Angewandte Chemie International Edition, Vol. 57, Issue 32
Photobiocatalysis: Activating Redox Enzymes by Direct or Indirect Transfer of Photoinduced Electrons
journal, May 2018
- Lee, Sahng Ha; Choi, Da Som; Kuk, Su Keun
- Angewandte Chemie International Edition, Vol. 57, Issue 27
Role of Biocatalysis in Sustainable Chemistry
journal, September 2017
- Sheldon, Roger A.; Woodley, John M.
- Chemical Reviews, Vol. 118, Issue 2
Photoexcitation of flavoenzymes enables a stereoselective radical cyclization
journal, June 2019
- Biegasiewicz, Kyle F.; Cooper, Simon J.; Gao, Xin
- Science, Vol. 364, Issue 6446
A genetically encoded photosensitizer protein facilitates the rational design of a miniature photocatalytic CO2-reducing enzyme
journal, November 2018
- Liu, Xiaohong; Kang, Fuying; Hu, Cheng
- Nature Chemistry, Vol. 10, Issue 12
The Photolyase/Cryptochrome Family of Proteins as DNA Repair Enzymes and Transcriptional Repressors
journal, January 2017
- Kavakli, Ibrahim Halil; Baris, Ibrahim; Tardu, Mehmet
- Photochemistry and Photobiology, Vol. 93, Issue 1
Controlling the optical and catalytic properties of artificial metalloenzyme photocatalysts using chemogenetic engineering
journal, January 2022
- Zubi, Yasmine S.; Liu, Bingqing; Gu, Yifan
- Chemical Science, Vol. 13, Issue 5
‘Photosystem II: the water splitting enzyme of photosynthesis and the origin of oxygen in our atmosphere’
journal, January 2016
- Barber, James
- Quarterly Reviews of Biophysics, Vol. 49
Low carbon strategies for sustainable bio-alkane gas production and renewable energy
journal, January 2020
- Amer, Mohamed; Wojcik, Emilia Z.; Sun, Chenhao
- Energy & Environmental Science, Vol. 13, Issue 6
Energy transfer catalysis mediated by visible light: principles, applications, directions
journal, January 2018
- Strieth-Kalthoff, Felix; James, Michael J.; Teders, Michael
- Chemical Society Reviews, Vol. 47, Issue 19
Photo-Biocatalysis: Biotransformations in the Presence of Light
journal, April 2019
- Schmermund, Luca; Jurkaš, Valentina; Özgen, F. Feyza
- ACS Catalysis, Vol. 9, Issue 5
Photoenzymes: A Novel Class of Biological Catalysts
journal, December 1994
- Begley, Tadhg P.
- Accounts of Chemical Research, Vol. 27, Issue 12
Photoenzymatic enantioselective intermolecular radical hydroalkylation
journal, June 2020
- Huang, Xiaoqiang; Wang, Binju; Wang, Yajie
- Nature, Vol. 584, Issue 7819
Design and Evaluation of Artificial Hybrid Photoredox Biocatalysts
journal, August 2020
- Schwochert, Timothy D.; Cruz, Cole L.; Watters, John W.
- ChemBioChem, Vol. 21, Issue 21
Hydrocarbon Synthesis via Photoenzymatic Decarboxylation of Carboxylic Acids
journal, January 2019
- Zhang, Wuyuan; Ma, Ming; Huijbers, Mieke M. E.
- Journal of the American Chemical Society, Vol. 141, Issue 7
Light‐Driven Kinetic Resolution of α‐Functionalized Carboxylic Acids Enabled by an Engineered Fatty Acid Photodecarboxylase
journal, June 2019
- Xu, Jian; Hu, Yujing; Fan, Jiajie
- Angewandte Chemie International Edition, Vol. 58, Issue 25
Quaternary Charge-Transfer Complex Enables Photoenzymatic Intermolecular Hydroalkylation of Olefins
journal, December 2020
- Page, Claire G.; Cooper, Simon J.; DeHovitz, Jacob S.
- Journal of the American Chemical Society, Vol. 143, Issue 1
Biocatalysis: Enzymatic Synthesis for Industrial Applications
journal, August 2020
- Wu, Shuke; Snajdrova, Radka; Moore, Jeffrey C.
- Angewandte Chemie International Edition, Vol. 60, Issue 1
P HOTOSYSTEM I: Function and Physiology
journal, June 2001
- Chitnis, Parag R.
- Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 52, Issue 1
Light-driven decarboxylative deuteration enabled by a divergently engineered photodecarboxylase
journal, June 2021
- Xu, Jian; Fan, Jiajie; Lou, Yujiao
- Nature Communications, Vol. 12, Issue 1
Artificial Metalloenzymes: Reaction Scope and Optimization Strategies
journal, May 2017
- Schwizer, Fabian; Okamoto, Yasunori; Heinisch, Tillmann
- Chemical Reviews, Vol. 118, Issue 1
Accessing non-natural reactivity by irradiating nicotinamide-dependent enzymes with light
journal, December 2016
- Emmanuel, Megan A.; Greenberg, Norman R.; Oblinsky, Daniel G.
- Nature, Vol. 540, Issue 7633
Static to inducibly dynamic stereocontrol: The convergent use of racemic β-substituted ketones
journal, August 2020
- DeHovitz, Jacob S.; Loh, Yong Yao; Kautzky, Jacob A.
- Science, Vol. 369, Issue 6507
Asymmetric redox-neutral radical cyclization catalysed by flavin-dependent ‘ene’-reductases
journal, December 2019
- Black, Michael J.; Biegasiewicz, Kyle F.; Meichan, Andrew J.
- Nature Chemistry, Vol. 12, Issue 1
Industrial applications of enzyme biocatalysis: Current status and future aspects
journal, November 2015
- Choi, Jung-Min; Han, Sang-Soo; Kim, Hak-Sung
- Biotechnology Advances, Vol. 33, Issue 7
Visible light photoredox catalysis: applications in organic synthesis
journal, January 2011
- Narayanam, Jagan M. R.; Stephenson, Corey R. J.
- Chem. Soc. Rev., Vol. 40, Issue 1