Efficient delivery of a DNA aptamer-based biosensor into plant cells for glucose sensing through thiol-mediated uptake

Mou, Quanbing; Xue, Xueyi; Ma, Yuan; Banik, Mandira; Garcia, Valeria; Guo, Weijie; Wang, Jiang; Song, Tingjie; Chen, Li-Qing; Lu, Yi

doi:10.1126/sciadv.abo0902

Title: Efficient delivery of a DNA aptamer-based biosensor into plant cells for glucose sensing through thiol-mediated uptake

Journal Article · Wed Jun 29 00:00:00 UTC 2022 · Science Advances

DOI: https://doi.org/10.1126/sciadv.abo0902 · OSTI ID:1902719

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^[2]; Ma, Yuan ^[3];

^[3]; Garcia, Valeria ^[3];

^[3]; Wang, Jiang ^[2]; Song, Tingjie ^[2];

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Univ. of Illinois at Urbana-Champaign, IL (United States); Univ. of Texas, Austin, TX (United States); University of Illinois
Univ. of Illinois at Urbana-Champaign, IL (United States)
Univ. of Illinois at Urbana-Champaign, IL (United States); Univ. of Texas, Austin, TX (United States)

DNA aptamers have been widely used as biosensors for detecting a variety of targets. Despite decades of success, they have not been applied to monitor any targets in plants, even though plants are a major platform for providing oxygen, food, and sustainable products ranging from energy fuels to chemicals, and high-value products such as pharmaceuticals. A major barrier to progress is a lack of efficient methods to deliver DNA into plant cells. We herein report a thiol-mediated uptake method that more efficiently delivers DNA into Arabidopsis and tobacco leaf cells than another state-of-the-art method, DNA nanostructures. Such a method allowed efficient delivery of a glucose DNA aptamer sensor into Arabidopsis for sensing glucose. This demonstration opens a new avenue to apply DNA aptamer sensors for functional studies of various targets, including metabolites, plant hormones, metal ions, and proteins in plants for a better understanding of the biodistribution and regulation of these species and their functions.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018420

OSTI ID:: 1902719

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 26 Vol. 8; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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