Title: Identification of xylan arabinosyl 2‐ O ‐xylosyltransferases catalyzing the addition of 2‐ O ‐xylosyl residue onto arabinosyl side chains of xylan in grass species

Abstract

SUMMARY Grass xylan, the major hemicellulose in both primary and secondary cell walls, is heavily decorated with α‐1,3‐linked arabinofuranosyl (Ara f ) residues that may be further substituted at O ‐2 with xylosyl (Xyl) or Ara f residues. Although xylan 3‐ O ‐arabinosyltransferases (XATs) catalyzing 3‐ O ‐Ara f addition onto xylan have been characterized, glycosyltransferases responsible for the transfer of 2‐ O ‐Xyl or 2‐ O ‐Ara f onto 3‐ O ‐Ara f residues of xylan to produce the Xyl‐Ara f and Ara f ‐Ara f disaccharide side chains remain to be identified. In this report, we showed that a rice GT61 member, named OsXAXT1 (xylan arabinosyl 2‐ O ‐xylosyltransferase 1) herein, was able to mediate the addition of Xyl‐Ara f disaccharide side chains onto xylan when heterologously co‐expressed with OsXAT2 in the Arabidopsis gux1/2/3 ( glucuronic acid substitution of xylan 1/2/3 ) triple mutant that lacks any glycosyl substitutions. Recombinant OsXAXT1 protein expressed in human embryonic kidney 293 cells exhibited a xylosyltransferase activity catalyzing the addition of Xyl from UDP‐Xyl onto arabinosylated xylooligomers. Consistent with its function as a xylan arabinosyl 2‐ O ‐xylosyltransferase, CRISPR‐Cas9‐mediated mutations of the OsXAXT1 gene in transgenic rice plants resulted in a reductionmore » in the level of Xyl‐Ara f disaccharide side chains in xylan. Furthermore, we revealed that XAXT1 close homologs from several other grass species, including switchgrass, maize, and Brachypodium , possessed the same functions as OsXAXT1, indicating functional conservation of XAXTs in grass species. Together, our findings establish that grass XAXTs are xylosyltransferases catalyzing Xyl transfer onto O ‐2 of Ara f residues of xylan to form the Xyl‐Ara f disaccharide side chains, which furthers our understanding of genes involved in xylan biosynthesis.« less

Authors:

Zhong, Ruiqin ^[1]; Lee, Chanhui ^[2]; Cui, Dongtao ^[3]; Phillips, Dennis R. ^[4]; Adams, Earle R. ^[4]; Jeong, Ho‐Young ^[2]; Jung, Ki‐Hong ^[5]; Ye, Zheng‐Hua  ^[1]

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+ Show Author Affiliations

1. Department of Plant Biology University of Georgia Athens GA 30602 USA
2. Department of Plant &, Environmental New Resources, College of Life Sciences Kyung Hee University Yongin 17104 Republic of Korea
3. Department of Chemistry and Chemical Biology Harvard University Cambridge MA 02138 USA
4. Department of Chemistry University of Georgia Athens GA 30602 USA
5. Graduate School of Green‐Bio Science Kyung Hee University Yongin 17104 Republic of Korea

Publication Date:

Mon Sep 05 00:00:00 EDT 2022

Sponsoring Org.:

USDOE

OSTI Identifier:

1890550

Grant/Contract Number:  

DE‐FG02‐03ER15415

Resource Type:

Publisher's Accepted Manuscript

Journal Name:

The Plant Journal

Additional Journal Information:

Journal Name: The Plant Journal Journal Volume: 112 Journal Issue: 1; Journal ID: ISSN 0960-7412

Publisher:

Wiley-Blackwell

Country of Publication:

United Kingdom

Language:

English