Biosynthesis of polycyclopropanated high energy biofuels

Cruz-Morales, Pablo; Yin, Kevin; Landera, Alexander; Cort, John R.; Young, Robert P.; Kyle, Jennifer E.; Bertrand, Robert; Iavarone, Anthony T.; Acharya, Suneil; Cowan, Aidan; Chen, Yan; Gin, Jennifer W.; Scown, Corinne Donahue; Petzold, Christopher J.; Araujo-Barcelos, Carolina; Sundstrom, Eric R.; George, Anthe; Liu, Yuzhong; Klass, Sarah H.; Nava, Alberto A.; Keasling, Jay D.

doi:10.1016/j.joule.2022.05.011

Title: Biosynthesis of polycyclopropanated high energy biofuels

Journal Article · Thu Jun 30 00:00:00 UTC 2022 · Joule

DOI: https://doi.org/10.1016/j.joule.2022.05.011 · OSTI ID:1886841

Cruz-Morales, Pablo ^[1]; Yin, Kevin ^[2]; Landera, Alexander ^[3];

^[4];

^[4]; Kyle, Jennifer E. ^[4]; Bertrand, Robert ^[5]; Iavarone, Anthony T. ^[6]; Acharya, Suneil ^[7]; Cowan, Aidan ^[2]; Chen, Yan ^[5]; Gin, Jennifer W. ^[5]; Scown, Corinne Donahue ^[8];

^[5]; Araujo-Barcelos, Carolina ^[9]; Sundstrom, Eric R. ^[9]; George, Anthe ^[3]; Liu, Yuzhong ^[5]; Klass, Sarah H. ^[8]; Nava, Alberto A. ^[2] more »

Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States); Technical Univ. of Denmark, Lyngby (Denmark)
Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States); Shenzhen Institute for Advanced Technologies (China); Technical Univ. of Denmark, Lyngby (Denmark)

Cyclopropane-functionalized hydrocarbons are excellent fuels due their high energy density. However, the organic synthesis of these molecules is challenging and harmful to the environment. In this work we produced polycyclopropanated fatty acids in bacteria. These molecules can be easily converted into renewable fuels for high energy applications such as shipping, long-haul transport, aviation, and rocketry. We explored the chemical diversity encoded in the genome of thousands of bacteria to identify and repurpose naturally occurring cyclopropanated molecules. We identified a set of candidate iterative polyketide synthases (iPKSs) predicted to produce polycyclopropanated fatty acids (POP-FAs), expressed these PKSs in Streptomyces coelicolor and produced the POP-FAs. We determined the structure of the molecules and increased their production 22-fold. Polycyclopropanated fatty acid methyl esters (POP-FAMEs) were obtained by methyl esterifying the POP-FAs. Finally, we calculated that our POP fuel candidates can have energy densities of more than 50 MJ/L. Our research shows that POP-FAMEs and other polyketide derived POPs have the energetic properties for energy-demanding applications for which sustainable alternatives are scarce.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: National Council of Science and Technology (CONACYT); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Vehicle Technologies Office; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830; NA0003525

OSTI ID:: 1886841

Report Number(s):: PNNL-SA-170223

Journal Information:: Joule, Journal Name: Joule Journal Issue: 7 Vol. 6; ISSN 2542-4351

Publisher:: Elsevier - Cell PressCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Biosynthesis of polycyclopropanated high energy biofuels

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