The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune

Marian, Ioana M.; Vonk, Peter Jan; Valdes, Ivan D.; Barry, Kerrie; Bostock, Benedict; Carver, Akiko; Daum, Chris; Lerner, Harry; Lipzen, Anna; Park, Hongjae; Schuller, Margo B. P.; Tegelaar, Martin; Tritt, Andrew; Schmutz, Jeremy; Grimwood, Jane; Lugones, Luis G.; Choi, In-Geol; Wösten, Han B.; Grigoriev, Igor V.; Ohm, Robin A.

doi:10.1128/mbio.00628-22

Title: The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune

Journal Article · Mon May 23 00:00:00 UTC 2022 · mBio (Online)

DOI: https://doi.org/10.1128/mbio.00628-22 · OSTI ID:1878514

Marian, Ioana M. ^[1]; Vonk, Peter Jan ^[1]; Valdes, Ivan D. ^[1]; Barry, Kerrie ^[2]; Bostock, Benedict ^[1]; Carver, Akiko ^[2]; Daum, Chris ^[2]; Lerner, Harry ^[1]; Lipzen, Anna ^[2]; Park, Hongjae ^[3]; Schuller, Margo B. P. ^[1]; Tegelaar, Martin ^[1]; Tritt, Andrew ^[2]; Schmutz, Jeremy ^[4]; Grimwood, Jane ^[4]; Lugones, Luis G. ^[1];

^[3]; Wösten, Han B. ^[1]; Grigoriev, Igor V. ^[5];

^[6]

Utrecht University (Netherlands)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Korea Univ., Seoul, (Korea, Republic of)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Utrecht University (Netherlands); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Wood-decaying fungi of the class Agaricomycetes (phylum Basidiomycota) are saprotrophs that break down lignocellulose and play an important role in nutrient recycling. They secrete a wide range of extracellular plant cell wall degrading enzymes that break down cellulose, hemicellulose, and lignin, the main building blocks of plant biomass. Although the production of these enzymes is regulated mainly at the transcriptional level, no activating regulators have been identified in any wood-decaying fungus in the class Agaricomycetes. We studied the regulation of cellulase expression in the wood-decaying fungus Schizophyllum commune. Comparative genomics and transcriptomics on two wild isolates revealed a Zn₂Cys₆-type transcription factor gene (roc1) that was highly upregulated during growth on cellulose, compared to glucose. It is only conserved in the class Agaricomycetes. A roc1 knockout strain showed an inability to grow on medium with cellulose as sole carbon source, and growth on cellobiose and xylan (other components of wood) was inhibited. Growth on non-wood-related carbon sources was not inhibited. Cellulase gene expression and enzyme activity were reduced in the Δroc1 strain. ChIP-Seq identified 1474 binding sites of the Roc1 transcription factor. Promoters of genes involved in lignocellulose degradation were enriched with these binding sites, especially those of LPMO (lytic polysaccharide monooxygenase) CAZymes, indicating that Roc1 directly regulates these genes. A conserved motif was identified as the binding site of Roc1, which was confirmed by a functional promoter analysis. Together, Roc1 is a key regulator of cellulose degradation and the first identified in wood-decaying fungi in the phylum Basidiomycota.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: European Research Council (ERC); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1878514

Journal Information:: mBio (Online), Journal Name: mBio (Online) Journal Issue: 3 Vol. 13; ISSN 2150-7511

Publisher:: American Society for Microbiology (ASM)Copyright Statement

Country of Publication:: United States

Language:: English

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Title: The Transcription Factor Roc1 Is a Key Regulator of Cellulose Degradation in the Wood-Decaying Mushroom Schizophyllum commune

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