The Ahr2-Dependent wfikkn1 Gene Influences Zebrafish Transcriptome, Proteome, and Behavior

Shankar, Prarthana; Garcia, Gloria R.; La Du, Jane K.; Sullivan, Christopher M.; Dunham, Cheryl L.; Goodale, Britton C.; Waters, Katrina M.; Stanisheuski, Stanislau; Maier, Claudia S.; Thunga, Preethi; Reif, David M.; Tanguay, Robyn L.

doi:10.1093/toxsci/kfac037

Title: The Ahr2-Dependent wfikkn1 Gene Influences Zebrafish Transcriptome, Proteome, and Behavior

Journal Article · Mon Apr 04 00:00:00 UTC 2022 · Toxicological Sciences

DOI: https://doi.org/10.1093/toxsci/kfac037 · OSTI ID:1878287

Shankar, Prarthana ^[1]; Garcia, Gloria R. ^[1]; La Du, Jane K. ^[1]; Sullivan, Christopher M. ^[1];

^[1]; Goodale, Britton C. ^[2];

^[3]; Stanisheuski, Stanislau ^[4]; Maier, Claudia S. ^[4]; Thunga, Preethi ^[5];

^[5];

^[1]

Oregon State Univ., Corvallis, OR (United States). The Sinnhuber Aquatic Research Laboratory
Oregon State Univ., Corvallis, OR (United States). The Sinnhuber Aquatic Research Laboratory; Dartmouth College, Lebanon, NH (United States). Geisel School of Medicine at Dartmouth, Norris Cotton Cancer Center
Oregon State Univ., Corvallis, OR (United States). The Sinnhuber Aquatic Research Laboratory; Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Oregon State Univ., Corvallis, OR (United States)
North Carolina State Univ., Raleigh, NC (United States). Bioinformatics Research Center

The aryl hydrocarbon receptor (AHR) is required for vertebrate development and is also activated by exogenous chemicals, including polycyclic aromatic hydrocarbons (PAHs) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). AHR activation is well-understood, but roles of downstream molecular signaling events are largely unknown. From previous transcriptomics in 48 h postfertilization (hpf) zebrafish exposed to several PAHs and TCDD, we found wfikkn1 was highly coexpressed with cyp1a (marker for AHR activation). Thus, we hypothesized wfikkn1’s role in AHR signaling, and showed that wfikkn1 expression was Ahr2 (zebrafish ortholog of human AHR)-dependent in developing zebrafish exposed to TCDD. To functionally characterize wfikkn1, we made a CRISPR-Cas9 mutant line with a 16-bp deletion in wfikkn1’s exon, and exposed wildtype and mutants to dimethyl sulfoxide or TCDD. 48-hpf mRNA sequencing revealed over 700 genes that were differentially expressed (p < .05, log₂FC > 1) between each pair of treatment combinations, suggesting an important role for wfikkn1 in altering both the 48-hpf transcriptome and TCDD-induced expression changes. Mass spectrometry-based proteomics of 48-hpf wildtype and mutants revealed 325 significant differentially expressed proteins. Functional enrichment demonstrated wfikkn1 was involved in skeletal muscle development and played a role in neurological pathways after TCDD exposure. Mutant zebrafish appeared morphologically normal but had significant behavior deficiencies at all life stages, and absence of Wfikkn1 did not significantly alter TCDD-induced behavior effects at all life stages. In conclusion, wfikkn1 did not appear to be significantly involved in TCDD’s overt toxicity but is likely a necessary functional member of the AHR signaling cascade.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1878287

Report Number(s):: PNNL-SA-169011

Journal Information:: Toxicological Sciences, Journal Name: Toxicological Sciences Journal Issue: 2 Vol. 187; ISSN 1096-6080

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
TCDD
aryl hydrocarbon receptor
behavior
molecular, biochemical, and systems toxicology
transcriptomics
wfikkn1
zebrafish

Title: The Ahr2-Dependent wfikkn1 Gene Influences Zebrafish Transcriptome, Proteome, and Behavior

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