Enhanced neurite outgrowth on electrically conductive carbon aerogel substrates in the presence of an external electric field
Abstract
Previous works from our laboratory have firmly established that aerogels are a suitable substrate to elicit accelerated neurite extension. On non-conducting aerogels, in the presence of an externally-applied DC bias, neurons extended neurites which were preferentially aligned towards the anode. In this work, we sought to determine whether electrically-conductive carbon aerogels elicited a more robust alignment of neurites toward the anode than non-conductive aerogels due to the capacity of conductive aerogels to sustain a current, thereby providing a direct interface between neurons and the external electrical stimulus. To determine if this was the case, we plated PC12 neuronal cells on electrically conductive carbon aerolges derived from acetic acid-catalized resorcinol formaldehyde aerogels (ARF-CA) and subjected them to an external electric field. The voltages applied at the electrodes of the custom-built electro-stimulation chamber were 0 V, 15 V, and 30 V. For each voltage, the directionality and length of the neurites extended by PC12 cells were determined and compared to those observed when PC12 cells were plated on non-conductive aerogels subjected to the same voltage. The results show that the directionality of neurite extension was similar between conductive and non-conductive aerogels. A higher neurite length difference was observed on conductive aerogels withmore »
- Authors:
-
- University of Memphis, TN (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1873645
- Alternate Identifier(s):
- OSTI ID: 1773185
- Report Number(s):
- LLNL-JRNL-835262
Journal ID: ISSN 1744-683X; 1053735
- Grant/Contract Number:
- AC52-07NA27344; 16-ERD-051; 13-LW099; 13-LW-099
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Soft Matter
- Additional Journal Information:
- Journal Volume: 17; Journal Issue: 17; Journal ID: ISSN 1744-683X
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Rodriguez Sala, Martina, Chandrasekaran, Swetha, Skalli, Omar, Worsley, Marcus, and Sabri, Firouzeh. Enhanced neurite outgrowth on electrically conductive carbon aerogel substrates in the presence of an external electric field. United States: N. p., 2021.
Web. doi:10.1039/d1sm00183c.
Rodriguez Sala, Martina, Chandrasekaran, Swetha, Skalli, Omar, Worsley, Marcus, & Sabri, Firouzeh. Enhanced neurite outgrowth on electrically conductive carbon aerogel substrates in the presence of an external electric field. United States. https://doi.org/10.1039/d1sm00183c
Rodriguez Sala, Martina, Chandrasekaran, Swetha, Skalli, Omar, Worsley, Marcus, and Sabri, Firouzeh. Mon .
"Enhanced neurite outgrowth on electrically conductive carbon aerogel substrates in the presence of an external electric field". United States. https://doi.org/10.1039/d1sm00183c. https://www.osti.gov/servlets/purl/1873645.
@article{osti_1873645,
title = {Enhanced neurite outgrowth on electrically conductive carbon aerogel substrates in the presence of an external electric field},
author = {Rodriguez Sala, Martina and Chandrasekaran, Swetha and Skalli, Omar and Worsley, Marcus and Sabri, Firouzeh},
abstractNote = {Previous works from our laboratory have firmly established that aerogels are a suitable substrate to elicit accelerated neurite extension. On non-conducting aerogels, in the presence of an externally-applied DC bias, neurons extended neurites which were preferentially aligned towards the anode. In this work, we sought to determine whether electrically-conductive carbon aerogels elicited a more robust alignment of neurites toward the anode than non-conductive aerogels due to the capacity of conductive aerogels to sustain a current, thereby providing a direct interface between neurons and the external electrical stimulus. To determine if this was the case, we plated PC12 neuronal cells on electrically conductive carbon aerolges derived from acetic acid-catalized resorcinol formaldehyde aerogels (ARF-CA) and subjected them to an external electric field. The voltages applied at the electrodes of the custom-built electro-stimulation chamber were 0 V, 15 V, and 30 V. For each voltage, the directionality and length of the neurites extended by PC12 cells were determined and compared to those observed when PC12 cells were plated on non-conductive aerogels subjected to the same voltage. The results show that the directionality of neurite extension was similar between conductive and non-conductive aerogels. A higher neurite length difference was observed on conductive aerogels with increasing voltage, 43% and 106% for 0–15 V and 0–30 V respectively, compared to non-conductive aerogels, 12% and 20%. These findings indicate that conductive carbon aerogels have a greater potential as scaffolds for nerve regeneration than non-conductive ones.},
doi = {10.1039/d1sm00183c},
journal = {Soft Matter},
number = 17,
volume = 17,
place = {United States},
year = {Mon Mar 29 00:00:00 EDT 2021},
month = {Mon Mar 29 00:00:00 EDT 2021}
}
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