Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers
Abstract
Enzyme-linked immunosorbent assays (ELISAs) are widely employed for the detection of protein targets due to their ease of use, sensitivity, and potential for high-throughput analyses. However, the use of ELISAs to detect non-protein targets such as lipids and amphiphiles is complicated by the physical properties of these molecules, which affects their association with functional surfaces and recognition ligands. Here, we developed a unique lipoprotein capture ELISA in which the natural association between lipoproteins and amphiphilic molecules facilitates detection of the target biomarker in a physiologically relevant conformation. An assay to detect the glycolipid lipoarabinomannan (LAM), a cell membrane component and virulence factor associated with Mycobacterial infections, was developed as a proof of concept.
- Publication Date:
- Research Org.:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1870199
- Alternate Identifier(s):
- OSTI ID: 1894847
- Report Number(s):
- LA-UR-22-22209
Journal ID: ISSN 0003-2697; S0003269722002032; 114747; PII: S0003269722002032
- Grant/Contract Number:
- 20200300ER; 89233218CNA000001
- Resource Type:
- Published Article
- Journal Name:
- Analytical Biochemistry
- Additional Journal Information:
- Journal Name: Analytical Biochemistry Journal Volume: 652 Journal Issue: C; Journal ID: ISSN 0003-2697
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biological science; lipoprotein; enzyme-linked immunosorbent assay; amphiphile; detection; lipoarabinomannan; nanodisc
Citation Formats
Lenz, Kiersten D., Klosterman, Katja E., Mukundan, Harshini, and Kubicek-Sutherland, Jessica Z. Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers. United States: N. p., 2022.
Web. doi:10.1016/j.ab.2022.114747.
Lenz, Kiersten D., Klosterman, Katja E., Mukundan, Harshini, & Kubicek-Sutherland, Jessica Z. Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers. United States. https://doi.org/10.1016/j.ab.2022.114747
Lenz, Kiersten D., Klosterman, Katja E., Mukundan, Harshini, and Kubicek-Sutherland, Jessica Z. Thu .
"Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers". United States. https://doi.org/10.1016/j.ab.2022.114747.
@article{osti_1870199,
title = {Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers},
author = {Lenz, Kiersten D. and Klosterman, Katja E. and Mukundan, Harshini and Kubicek-Sutherland, Jessica Z.},
abstractNote = {Enzyme-linked immunosorbent assays (ELISAs) are widely employed for the detection of protein targets due to their ease of use, sensitivity, and potential for high-throughput analyses. However, the use of ELISAs to detect non-protein targets such as lipids and amphiphiles is complicated by the physical properties of these molecules, which affects their association with functional surfaces and recognition ligands. Here, we developed a unique lipoprotein capture ELISA in which the natural association between lipoproteins and amphiphilic molecules facilitates detection of the target biomarker in a physiologically relevant conformation. An assay to detect the glycolipid lipoarabinomannan (LAM), a cell membrane component and virulence factor associated with Mycobacterial infections, was developed as a proof of concept.},
doi = {10.1016/j.ab.2022.114747},
journal = {Analytical Biochemistry},
number = C,
volume = 652,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 2022},
month = {Thu Sep 01 00:00:00 EDT 2022}
}
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