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Title: Structure of a photosystem I-ferredoxin complex from a marine cyanobacterium provides insights into far-red light photoacclimation

Abstract

Far-red light photoacclimation (FaRLiP) exhibited by some cyanobacteria allows these organisms to use light of the far-red region of the solar spectrum (700 to 800 nm) for photosynthesis. Part of this process includes the replacement of six photosystem I (PSI) subunits with isoforms that confer the binding of chlorophyll (Chl) f molecules that are synthesized by chlorophyll f synthase and that absorb far-red light. However, the exact sites at which Chl f molecules are bound are still challenging to determine. To aid in the identification of Chl fbinding sites, we solved the cryo-EM structure of PSI from far-red light-acclimated cells (FRLPSI) of the cyanobacterium Synechococcus sp. PCC 7335. We identified six sites that bind Chl f with high specificity, and three additional sites that are likely to bind Chl f at lower specificity. All of these binding sites are in the core antenna regions of PSI, and Chl f was not observed among the electron transport cofactors. This structural analysis also reveals both conserved and non-conserved Chl f-binding sites, the latter of which exemplify the diversity in FRL-PSI among species. We found that the FRL-PSI structure also contains a bound soluble ferredoxin, PetF1, at low occupancy, which suggests that ferredoxinmore » binds less transiently than expected according to the canonical view of ferredoxin-binding to facilitate electron transfer. We suggest that this may result from structural changes in FRL-PSI that occur specifically during FaRLiP.« less

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [1]; ORCiD logo [2]
+ Show Author Affiliations
  1. Yale Univ., New Haven, CT (United States)
  2. Pennsylvania State Univ., University Park, PA (United States)
  3. National Taiwan Univ., Taipei (Taiwan)
Publication Date:
Research Org.:
Yale Univ., New Haven, CT (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
OSTI Identifier:
1860617
Grant/Contract Number:  
FG02-05ER15646
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 298; Journal Issue: 1; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; photosystem I; far-red light photoacclimation; chlorophyll f; ferredoxin; PsaF; PsaJ; photosynthesis; cyanobacteria

Citation Formats

Gisriel, Christopher J., Flesher, David A., Shen, Gaozhong, Wang, Jimin, Ho, Ming-Yang, Brudvig, Gary W., and Bryant, Donald A. Structure of a photosystem I-ferredoxin complex from a marine cyanobacterium provides insights into far-red light photoacclimation. United States: N. p., 2021. Web. doi:10.1016/j.jbc.2021.101408.
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Gisriel, Christopher J., Flesher, David A., Shen, Gaozhong, Wang, Jimin, Ho, Ming-Yang, Brudvig, Gary W., & Bryant, Donald A. Structure of a photosystem I-ferredoxin complex from a marine cyanobacterium provides insights into far-red light photoacclimation. United States. https://doi.org/10.1016/j.jbc.2021.101408
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Gisriel, Christopher J., Flesher, David A., Shen, Gaozhong, Wang, Jimin, Ho, Ming-Yang, Brudvig, Gary W., and Bryant, Donald A. Mon . "Structure of a photosystem I-ferredoxin complex from a marine cyanobacterium provides insights into far-red light photoacclimation". United States. https://doi.org/10.1016/j.jbc.2021.101408. https://www.osti.gov/servlets/purl/1860617.
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@article{osti_1860617,
title = {Structure of a photosystem I-ferredoxin complex from a marine cyanobacterium provides insights into far-red light photoacclimation},
author = {Gisriel, Christopher J. and Flesher, David A. and Shen, Gaozhong and Wang, Jimin and Ho, Ming-Yang and Brudvig, Gary W. and Bryant, Donald A.},
abstractNote = {Far-red light photoacclimation (FaRLiP) exhibited by some cyanobacteria allows these organisms to use light of the far-red region of the solar spectrum (700 to 800 nm) for photosynthesis. Part of this process includes the replacement of six photosystem I (PSI) subunits with isoforms that confer the binding of chlorophyll (Chl) f molecules that are synthesized by chlorophyll f synthase and that absorb far-red light. However, the exact sites at which Chl f molecules are bound are still challenging to determine. To aid in the identification of Chl fbinding sites, we solved the cryo-EM structure of PSI from far-red light-acclimated cells (FRLPSI) of the cyanobacterium Synechococcus sp. PCC 7335. We identified six sites that bind Chl f with high specificity, and three additional sites that are likely to bind Chl f at lower specificity. All of these binding sites are in the core antenna regions of PSI, and Chl f was not observed among the electron transport cofactors. This structural analysis also reveals both conserved and non-conserved Chl f-binding sites, the latter of which exemplify the diversity in FRL-PSI among species. We found that the FRL-PSI structure also contains a bound soluble ferredoxin, PetF1, at low occupancy, which suggests that ferredoxin binds less transiently than expected according to the canonical view of ferredoxin-binding to facilitate electron transfer. We suggest that this may result from structural changes in FRL-PSI that occur specifically during FaRLiP.},
doi = {10.1016/j.jbc.2021.101408},
journal = {Journal of Biological Chemistry},
number = 1,
volume = 298,
place = {United States},
year = {Mon Nov 15 00:00:00 EST 2021},
month = {Mon Nov 15 00:00:00 EST 2021}
}
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https://doi.org/10.1016/j.jbc.2021.101408
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