A Genetic Study of Nif -Associated Genes in a Hyperthermophilic Methanogen

Lie, Thomas J.; Kuo, Yang P.; Leite, Mara; Costa, Kyle C.; Harwood, Caroline S.; Leigh, John A.; Sonnenschein, ed., Eva C.

doi:10.1128/spectrum.02093-21

Title: A Genetic Study of Nif -Associated Genes in a Hyperthermophilic Methanogen

Journal Article · 22 February 2022 · Microbiology Spectrum

DOI: https://doi.org/10.1128/spectrum.02093-21 · OSTI ID:1843283

Lie, Thomas J. ^[1]; Kuo, Yang P. ^[1]; Leite, Mara ^[1];

^[1];

^[1]; Leigh, John A. ^[1]; Sonnenschein, ed., Eva C.

Department of Microbiology, University of Washington, Seattle, Washington, USA

Methanocaldococcus has been considered a model genus, but genetic tools have not been forthcoming until recently. Here, we develop and illustrate the utility of positive selection with either of two selective agents (simvastatin and neomycin), negative selection, generation of markerless in-frame deletion mutations, and genetic complementation.

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Research Organization:: Univ. of Washington, Seattle, WA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: FG02-05ER15709

OSTI ID:: 1843283

Journal Information:: Microbiology Spectrum, Journal Name: Microbiology Spectrum Journal Issue: 1 Vol. 10; ISSN 2165-0497

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Genetics
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Microbiology
Nitrogen fixation

Title: A Genetic Study of Nif -Associated Genes in a Hyperthermophilic Methanogen

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