Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms
Abstract
Isoprenoid compounds are biologically ubiquitous, and their characteristic modularity has afforded products ranging from pharmaceuticals to biofuels. Isoprenoid production has been largely successful in Escherichia coli and Saccharomyces cerevisiae with metabolic engineering of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways coupled with the expression of heterologous terpene synthases. Yet conventional microbial chassis pose several major obstacles to successful commercialization including the affordability of sugar substrates at scale, precursor flux limitations, and intermediate feedback-inhibition. Now, recent studies have challenged typical isoprenoid paradigms by expanding the boundaries of terpene biosynthesis and using non-model organisms including those capable of metabolizing atypical C1 substrates. Conversely, investigations of non-model organisms have historically informed optimization in conventional microbes by tuning heterologous gene expression. Here, we review advances in isoprenoid biosynthesis with specific focus on the synergy between model and non-model organisms that may elevate the commercial viability of isoprenoid platforms by addressing the dichotomy between high titer production and inexpensive substrates.
- Authors:
- Publication Date:
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1833738
- Grant/Contract Number:
- AC0205CH11231
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Name: Frontiers in Microbiology Journal Volume: 12; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Media SA
- Country of Publication:
- Switzerland
- Language:
- English
Citation Formats
Carruthers, David N., and Lee, Taek Soon. Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms. Switzerland: N. p., 2021.
Web. doi:10.3389/fmicb.2021.791089.
Carruthers, David N., & Lee, Taek Soon. Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms. Switzerland. https://doi.org/10.3389/fmicb.2021.791089
Carruthers, David N., and Lee, Taek Soon. Thu .
"Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms". Switzerland. https://doi.org/10.3389/fmicb.2021.791089.
@article{osti_1833738,
title = {Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms},
author = {Carruthers, David N. and Lee, Taek Soon},
abstractNote = {Isoprenoid compounds are biologically ubiquitous, and their characteristic modularity has afforded products ranging from pharmaceuticals to biofuels. Isoprenoid production has been largely successful in Escherichia coli and Saccharomyces cerevisiae with metabolic engineering of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways coupled with the expression of heterologous terpene synthases. Yet conventional microbial chassis pose several major obstacles to successful commercialization including the affordability of sugar substrates at scale, precursor flux limitations, and intermediate feedback-inhibition. Now, recent studies have challenged typical isoprenoid paradigms by expanding the boundaries of terpene biosynthesis and using non-model organisms including those capable of metabolizing atypical C1 substrates. Conversely, investigations of non-model organisms have historically informed optimization in conventional microbes by tuning heterologous gene expression. Here, we review advances in isoprenoid biosynthesis with specific focus on the synergy between model and non-model organisms that may elevate the commercial viability of isoprenoid platforms by addressing the dichotomy between high titer production and inexpensive substrates.},
doi = {10.3389/fmicb.2021.791089},
journal = {Frontiers in Microbiology},
number = ,
volume = 12,
place = {Switzerland},
year = {Thu Dec 02 00:00:00 EST 2021},
month = {Thu Dec 02 00:00:00 EST 2021}
}
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