Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling
Abstract
Soil microorganisms determine the fate of soil organic matter (SOM), and their activities compose a major component of the global carbon (C) cycle. For this work, we employed a multisubstrate, DNA-stable isotope probing experiment to track bacterial assimilation of C derived from distinct sources that varied in bioavailability. This approach allowed us to measure microbial contributions to SOM processing by measuring the C assimilation dynamics of diverse microorganisms as they interacted within soil. We identified and tracked 1,286 bacterial taxa that assimilated 13C in an agricultural soil over a period of 48 d. Overall 13C-assimilation dynamics of bacterial taxa, defined by the source and timing of the 13C they assimilated, exhibited low phylogenetic conservation. We identified bacterial guilds composed of taxa that had similar 13C assimilation dynamics. We show that C-source bioavailability explained significant variation in both C mineralization dynamics and guild structure, and that the growth dynamics of bacterial guilds differed significantly in response to C addition. We also demonstrate that the guild structure explains significant variation in the biogeographical distribution of bacteria at continental and global scales. These results suggest that an understanding of in situ growth dynamics is essential for understanding microbial contributions to soil C cycling.more »
- Authors:
-
[1];
[1]
- School of Integrative Plant Science, Cornell University, Ithaca, NY 14853,
- School of Integrative Plant Science, Cornell University, Ithaca, NY 14853,, Department of Microbiome Science, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany,
- School of Integrative Plant Science, Cornell University, Ithaca, NY 14853,, Department of Biological Sciences, University of the Sciences, Philadelphia, PA 19104
- Publication Date:
- Research Org.:
- Cornell Univ., Ithaca, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS)
- OSTI Identifier:
- 1831533
- Alternate Identifier(s):
- OSTI ID: 1863898; OSTI ID: 2228485; OSTI ID: 2228924
- Grant/Contract Number:
- SC0004486; SC0010558; SC0016364
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 118 Journal Issue: 47; Journal ID: ISSN 0027-8424
- Publisher:
- Proceedings of the National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; DNA-SIP; bacterial; soil; carbon cycling; ecology
Citation Formats
Barnett, Samuel E., Youngblut, Nicholas D., Koechli, Chantal N., and Buckley, Daniel H. Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling. United States: N. p., 2021.
Web. doi:10.1073/pnas.2115292118.
Barnett, Samuel E., Youngblut, Nicholas D., Koechli, Chantal N., & Buckley, Daniel H. Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling. United States. https://doi.org/10.1073/pnas.2115292118
Barnett, Samuel E., Youngblut, Nicholas D., Koechli, Chantal N., and Buckley, Daniel H. Fri .
"Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling". United States. https://doi.org/10.1073/pnas.2115292118.
@article{osti_1831533,
title = {Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling},
author = {Barnett, Samuel E. and Youngblut, Nicholas D. and Koechli, Chantal N. and Buckley, Daniel H.},
abstractNote = {Soil microorganisms determine the fate of soil organic matter (SOM), and their activities compose a major component of the global carbon (C) cycle. For this work, we employed a multisubstrate, DNA-stable isotope probing experiment to track bacterial assimilation of C derived from distinct sources that varied in bioavailability. This approach allowed us to measure microbial contributions to SOM processing by measuring the C assimilation dynamics of diverse microorganisms as they interacted within soil. We identified and tracked 1,286 bacterial taxa that assimilated 13C in an agricultural soil over a period of 48 d. Overall 13C-assimilation dynamics of bacterial taxa, defined by the source and timing of the 13C they assimilated, exhibited low phylogenetic conservation. We identified bacterial guilds composed of taxa that had similar 13C assimilation dynamics. We show that C-source bioavailability explained significant variation in both C mineralization dynamics and guild structure, and that the growth dynamics of bacterial guilds differed significantly in response to C addition. We also demonstrate that the guild structure explains significant variation in the biogeographical distribution of bacteria at continental and global scales. These results suggest that an understanding of in situ growth dynamics is essential for understanding microbial contributions to soil C cycling. We interpret these findings in the context of bacterial life history strategies and their relationship to terrestrial C cycling.},
doi = {10.1073/pnas.2115292118},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 47,
volume = 118,
place = {United States},
year = {Fri Nov 19 00:00:00 EST 2021},
month = {Fri Nov 19 00:00:00 EST 2021}
}
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Works referenced in this record:
Global soil carbon: understanding and managing the largest terrestrial carbon pool
journal, February 2014
- Scharlemann, Jörn PW; Tanner, Edmund VJ; Hiederer, Roland
- Carbon Management, Vol. 5, Issue 1
Microbial Extracellular Enzymes and the Marine Carbon Cycle
journal, January 2011
- Arnosti, Carol
- Annual Review of Marine Science, Vol. 3, Issue 1
Quantitative assessment of microbial necromass contribution to soil organic matter
journal, August 2019
- Liang, Chao; Amelung, Wulf; Lehmann, Johannes
- Global Change Biology, Vol. 25, Issue 11
Growth of Marine Bacteria at Limiting Concentrations of Organic Carbon in Seawater1: Growth of Bacteria at low Substrate Levels
journal, April 1967
- Jannasch, Holger W.
- Limnology and Oceanography, Vol. 12, Issue 2
Interactions of Hydrophobic Fractions of Dissolved Organic Matter with Fe 3+ - and Cu 2+ -Montmorillonite
journal, May 2008
- Polubesova, Tamara; Chen, Yona; Navon, Rotem
- Environmental Science & Technology, Vol. 42, Issue 13
Microbial community utilization of added carbon substrates in response to long-term carbon input manipulation
journal, August 2006
- Brant, Justin B.; Sulzman, Elizabeth W.; Myrold, David D.
- Soil Biology and Biochemistry, Vol. 38, Issue 8
Intra-genomic variation in G + C content and its implications for DNA stable isotope probing: DNA-SIP and intra-genomic variation in G + C content
journal, October 2014
- Youngblut, Nicholas D.; Buckley, Daniel H.
- Environmental Microbiology Reports, Vol. 6, Issue 6
Viruses in soil: Nano-scale undead drivers of microbial life, biogeochemical turnover and ecosystem functions
journal, December 2018
- Kuzyakov, Yakov; Mason-Jones, Kyle
- Soil Biology and Biochemistry, Vol. 127
Combining the fourth-corner and the RLQ methods for assessing trait responses to environmental variation
journal, January 2014
- Dray, Stéphane; Choler, Philippe; Dolédec, Sylvain
- Ecology, Vol. 95, Issue 1
Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences
journal, August 2013
- Langille, Morgan G. I.; Zaneveld, Jesse; Caporaso, J. Gregory
- Nature Biotechnology, Vol. 31, Issue 9
Co-Inertia Analysis and the Linking of Ecological data Tables
journal, November 2003
- Dray, Stéphane; Chessel, Daniel; Thioulouse, Jean
- Ecology, Vol. 84, Issue 11
Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
journal, June 2013
- Kozich, James J.; Westcott, Sarah L.; Baxter, Nielson T.
- Applied and Environmental Microbiology, Vol. 79, Issue 17
Agricultural Management and Labile Carbon Additions Affect Soil Microbial Community Structure and Interact with Carbon and Nitrogen Cycling
journal, April 2013
- Berthrong, Sean T.; Buckley, Daniel H.; Drinkwater, Laurie E.
- Microbial Ecology, Vol. 66, Issue 1
Cultivation of unculturable soil bacteria
journal, September 2012
- Pham, Van H. T.; Kim, Jaisoo
- Trends in Biotechnology, Vol. 30, Issue 9
Microbiomes in light of traits: A phylogenetic perspective
journal, November 2015
- Martiny, J. B. H.; Jones, S. E.; Lennon, J. T.
- Science, Vol. 350, Issue 6261
Environmental and stoichiometric controls on microbial carbon-use efficiency in soils: Research review
journal, July 2012
- Manzoni, Stefano; Taylor, Philip; Richter, Andreas
- New Phytologist, Vol. 196, Issue 1
Soil Carbon Sequestration Impacts on Global Climate Change and Food Security
journal, June 2004
- Lal, R.
- Science, Vol. 304, Issue 5677
Effect of Anthropogenic Land-Use and Land-Cover Changes on Climate and Land Carbon Storage in CMIP5 Projections for the Twenty-First Century
journal, September 2013
- Brovkin, V.; Boysen, L.; Arora, V. K.
- Journal of Climate, Vol. 26, Issue 18
Functional Redundancy Supports Biodiversity and Ecosystem Function in a Closed and Constant Environment
journal, June 2004
- Wohl, Debra L.; Arora, Satyam; Gladstone, Jessica R.
- Ecology, Vol. 85, Issue 6
Rainfall-induced carbon dioxide pulses result from sequential resuscitation of phylogenetically clustered microbial groups
journal, June 2012
- Placella, S. A.; Brodie, E. L.; Firestone, M. K.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 27
The physiology and ecological implications of efficient growth
journal, January 2015
- Roller, Benjamin RK; Schmidt, Thomas M.
- The ISME Journal, Vol. 9, Issue 7
Phylogenetic conservatism of functional traits in microorganisms
journal, December 2012
- Martiny, Adam C.; Treseder, Kathleen; Pusch, Gordon
- The ISME Journal, Vol. 7, Issue 4
HTSSIP: An R package for analysis of high throughput sequencing data from nucleic acid stable isotope probing (SIP) experiments
journal, January 2018
- Youngblut, Nicholas D.; Barnett, Samuel E.; Buckley, Daniel H.
- PLOS ONE, Vol. 13, Issue 1
Growth responses of oligotrophic and heterotrophic marine bacteria in various substrate concentrations, and taxonomic studies on them
journal, July 1980
- Akagi, Y.; Simidu, U.; Taga, N.
- Canadian Journal of Microbiology, Vol. 26, Issue 7
SIPSim: A Modeling Toolkit to Predict Accuracy and Aid Design of DNA-SIP Experiments
journal, March 2018
- Youngblut, Nicholas D.; Barnett, Samuel E.; Buckley, Daniel H.
- Frontiers in Microbiology, Vol. 9
Phylogenetic Clustering and Overdispersion in Bacterial Communities
journal, July 2006
- Horner-Devine, M. Claire; Bohannan, Brendan J. M.
- Ecology, Vol. 87, Issue sp7
Embracing the unknown: disentangling the complexities of the soil microbiome
journal, August 2017
- Fierer, Noah
- Nature Reviews Microbiology, Vol. 15, Issue 10
Mapping the niche space of soil microorganisms using taxonomy and traits
journal, August 2012
- Lennon, Jay T.; Aanderud, Zachary T.; Lehmkuhl, B. K.
- Ecology, Vol. 93, Issue 8
Managing uncertainty in soil carbon feedbacks to climate change
journal, July 2016
- Bradford, Mark A.; Wieder, William R.; Bonan, Gordon B.
- Nature Climate Change, Vol. 6, Issue 8
Examining the global distribution of dominant archaeal populations in soil
journal, November 2010
- Bates, Scott T.; Berg-Lyons, Donna; Caporaso, J. Gregory
- The ISME Journal, Vol. 5, Issue 5
High proportions of bacteria and archaea across most biomes remain uncultured
journal, August 2019
- Steen, Andrew D.; Crits-Christoph, Alexander; Carini, Paul
- The ISME Journal, Vol. 13, Issue 12
Quantitative Microbial Ecology through Stable Isotope Probing
journal, August 2015
- Hungate, Bruce A.; Mau, Rebecca L.; Schwartz, Egbert
- Applied and Environmental Microbiology, Vol. 81, Issue 21
Changes in soil organic carbon storage predicted by Earth system models during the 21st century
journal, January 2014
- Todd-Brown, K. E. O.; Randerson, J. T.; Hopkins, F.
- Biogeosciences, Vol. 11, Issue 8
Soil health and carbon management
journal, November 2016
- Lal, Rattan
- Food and Energy Security, Vol. 5, Issue 4
Cross-biome metagenomic analyses of soil microbial communities and their functional attributes
journal, December 2012
- Fierer, N.; Leff, J. W.; Adams, B. J.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 52
Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data: Fig. 1.
journal, May 2015
- Aßhauer, Kathrin P.; Wemheuer, Bernd; Daniel, Rolf
- Bioinformatics, Vol. 31, Issue 17
The Survival of Starved Bacteria
journal, October 1962
- Postgate, J. R.; Hunter, J. R.
- Journal of General Microbiology, Vol. 29, Issue 2
The Vertical Distribution of soil Organic Carbon and its Relation to Climate and Vegetation
journal, April 2000
- Jobbágy, Esteban G.; Jackson, Robert B.
- Ecological Applications, Vol. 10, Issue 2
Global soil carbon projections are improved by modelling microbial processes
journal, July 2013
- Wieder, William R.; Bonan, Gordon B.; Allison, Steven D.
- Nature Climate Change, Vol. 3, Issue 10
Matching species traits to environmental variables: a new three-table ordination method
journal, June 1996
- Dolédec, S.; Chessel, D.; ter Braak, C. J. F.
- Environmental and Ecological Statistics, Vol. 3, Issue 2
The role of ecological theory in microbial ecology
journal, May 2007
- Prosser, James I.; Bohannan, Brendan J. M.; Curtis, Tom P.
- Nature Reviews Microbiology, Vol. 5, Issue 5
Computation of Octanol−Water Partition Coefficients by Guiding an Additive Model with Knowledge
journal, September 2007
- Cheng, Tiejun; Zhao, Yuan; Li, Xun
- Journal of Chemical Information and Modeling, Vol. 47, Issue 6
Phylogenetically Novel Uncultured Microbial Cells Dominate Earth Microbiomes
journal, September 2018
- Lloyd, Karen G.; Steen, Andrew D.; Ladau, Joshua
- mSystems, Vol. 3, Issue 5
Evidence for the Existence of Three Primary Strategies in Plants and Its Relevance to Ecological and Evolutionary Theory
journal, November 1977
- Grime, J. P.
- The American Naturalist, Vol. 111, Issue 982
Stable isotope probing — linking microbial identity to function
journal, May 2005
- Dumont, Marc G.; Murrell, J. Colin
- Nature Reviews Microbiology, Vol. 3, Issue 6
Molecular-Level Interactions in Soils and Sediments: The Role of Aromatic π-Systems
journal, May 2009
- Keiluweit, Marco; Kleber, Markus
- Environmental Science & Technology, Vol. 43, Issue 10
Bioavailability in soil or sediment: exposure of different organisms and approaches to study it
journal, April 2000
- Sijm, D.; Kraaij, R.; Belfroid, A.
- Environmental Pollution, Vol. 108, Issue 1
Integrating microbial physiology and physio-chemical principles in soils with the MIcrobial-MIneral Carbon Stabilization (MIMICS) model
journal, January 2014
- Wieder, W. R.; Grandy, A. S.; Kallenbach, C. M.
- Biogeosciences, Vol. 11, Issue 14
Soil characteristics and land-use drive bacterial community assembly patterns
journal, December 2019
- Barnett, Samuel E.; Youngblut, Nicholas D.; Buckley, Daniel H.
- FEMS Microbiology Ecology, Vol. 96, Issue 1
Causes of variation in soil carbon simulations from CMIP5 Earth system models and comparison with observations
journal, January 2013
- Todd-Brown, K. E. O.; Randerson, J. T.; Post, W. M.
- Biogeosciences, Vol. 10, Issue 3
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009
- Schloss, P. D.; Westcott, S. L.; Ryabin, T.
- Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
A new view of the tree of life
journal, April 2016
- Hug, Laura A.; Baker, Brett J.; Anantharaman, Karthik
- Nature Microbiology, Vol. 1, Issue 5
Genomes from uncultivated prokaryotes: a comparison of metagenome-assembled and single-amplified genomes
journal, September 2018
- Alneberg, Johannes; Karlsson, Christofer M. G.; Divne, Anna-Maria
- Microbiome, Vol. 6, Issue 1
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
The Guild Concept and the Structure of Ecological Communities
journal, November 1991
- Simberloff, D.; Dayan, T.
- Annual Review of Ecology and Systematics, Vol. 22, Issue 1
Conceptualizing functional traits and ecological characteristics of methane-oxidizing bacteria as life strategies: Functional traits of methane-oxidizing bacteria
journal, August 2012
- Ho, Adrian; Kerckhof, Frederiek-Maarten; Luke, Claudia
- Environmental Microbiology Reports, Vol. 5, Issue 3
Nutrient Uptake by Microorganisms according to Kinetic Parameters from Theory as Related to Cytoarchitecture
journal, September 1998
- Button, D. K.
- Microbiology and Molecular Biology Reviews, Vol. 62, Issue 3
The role of soil organic matter in maintaining soil quality in continuous cropping systems
journal, November 1997
- Reeves, D. W.
- Soil and Tillage Research, Vol. 43, Issue 1-2
Microbial control over carbon cycling in soil
journal, January 2012
- Schimel, Joshua P.; Schaeffer, Sean M.
- Frontiers in Microbiology, Vol. 3
Microbiome Datasets Are Compositional: And This Is Not Optional
journal, November 2017
- Gloor, Gregory B.; Macklaim, Jean M.; Pawlowsky-Glahn, Vera
- Frontiers in Microbiology, Vol. 8
Microbe-driven turnover offsets mineral-mediated storage of soil carbon under elevated CO2
journal, November 2014
- Sulman, Benjamin N.; Phillips, Richard P.; Oishi, A. Christopher
- Nature Climate Change, Vol. 4, Issue 12
The All-Species Living Tree project: A 16S rRNA-based phylogenetic tree of all sequenced type strains
journal, September 2008
- Yarza, Pablo; Richter, Michael; Peplies, Jörg
- Systematic and Applied Microbiology, Vol. 31, Issue 4
Incorporating 16S Gene Copy Number Information Improves Estimates of Microbial Diversity and Abundance
journal, October 2012
- Kembel, Steven W.; Wu, Martin; Eisen, Jonathan A.
- PLoS Computational Biology, Vol. 8, Issue 10
Development of microbial-enzyme-mediated decomposition model parameters through steady-state and dynamic analyses
journal, January 2013
- Wang, Gangsheng; Post, Wilfred M.; Mayes, Melanie A.
- Ecological Applications, Vol. 23, Issue 1
Soils as carbon sinks: the global context
journal, June 2004
- Smith, P.; Smith, P.
- Soil Use and Management, Vol. 20, Issue 2
Explicitly representing soil microbial processes in Earth system models: Soil microbes in earth system models
journal, October 2015
- Wieder, William R.; Allison, Steven D.; Davidson, Eric A.
- Global Biogeochemical Cycles, Vol. 29, Issue 10
Bacterial Utilization of Low Concentrations of Organic Matter
journal, June 1943
- Zobell, Claude E.; Grant, Carroll W.
- Journal of Bacteriology, Vol. 45, Issue 6
Stabilization and destabilization of soil organic matter: mechanisms and controls
journal, November 1996
- Sollins, Phillip; Homann, Peter; Caldwell, Bruce A.
- Geoderma, Vol. 74, Issue 1-2
Strategies to improve reference databases for soil microbiomes
journal, December 2016
- Choi, Jinlyung; Yang, Fan; Stepanauskas, Ramunas
- The ISME Journal, Vol. 11, Issue 4
pplacer: linear time maximum-likelihood and Bayesian phylogenetic placement of sequences onto a fixed reference tree
journal, October 2010
- Matsen, Frederick A.; Kodner, Robin B.; Armbrust, E. Virginia
- BMC Bioinformatics, Vol. 11, Issue 1
Hierarchical self-assembly of 3D lattices from polydisperse anisometric colloids
journal, April 2019
- Luo, Binbin; Kim, Ahyoung; Smith, John W.
- Nature Communications, Vol. 10, Issue 1
Cultivating the uncultured: limits, advances and future challenges
journal, June 2009
- Alain, Karine; Querellou, Joël
- Extremophiles, Vol. 13, Issue 4
FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance Matrix
journal, April 2009
- Price, M. N.; Dehal, P. S.; Arkin, A. P.
- Molecular Biology and Evolution, Vol. 26, Issue 7
Nutrient-limited microbial growth kinetics: overview and recent advances
journal, January 1993
- Button, D. K.
- Antonie van Leeuwenhoek, Vol. 63, Issue 3-4
Unearthing the Ecology of Soil Microorganisms Using a High Resolution DNA-SIP Approach to Explore Cellulose and Xylose Metabolism in Soil
journal, May 2016
- Pepe-Ranney, Charles; Campbell, Ashley N.; Koechli, Chantal N.
- Frontiers in Microbiology, Vol. 7
Defining clusters from a hierarchical cluster tree: the Dynamic Tree Cut package for R
journal, November 2007
- Langfelder, Peter; Zhang, Bin; Horvath, Steve
- Bioinformatics, Vol. 24, Issue 5
Phylogenetic conservation of substrate use specialization in leaf litter bacteria
journal, March 2017
- Dolan, Kristin L.; Peña, Jeniffer; Allison, Steven D.
- PLOS ONE, Vol. 12, Issue 3
Terrestrial ecosystem carbon dynamics and climate feedbacks
journal, January 2008
- Heimann, Martin; Reichstein, Markus
- Nature, Vol. 451, Issue 7176
Picante: R tools for integrating phylogenies and ecology
journal, April 2010
- Kembel, S. W.; Cowan, P. D.; Helmus, M. R.
- Bioinformatics, Vol. 26, Issue 11
Defining the functional traits that drive bacterial decomposer community productivity
journal, March 2017
- Evans, Rachael; Alessi, Anna M.; Bird, Susannah
- The ISME Journal, Vol. 11, Issue 7
Changes in organic chemical components of needle litter during decomposition. Long-term decomposition in a Scots pine forest. I
journal, August 1982
- Berg, Björn; Hannus, Kai; Popoff, Thomas
- Canadian Journal of Botany, Vol. 60, Issue 8
Biology of Oligotrophic Bacteria
journal, October 1979
- Kuznetsov, S. I.; Dubinina, G. A.; Lapteva, N. A.
- Annual Review of Microbiology, Vol. 33, Issue 1
Predictability of the terrestrial carbon cycle
journal, December 2014
- Luo, Yiqi; Keenan, Trevor F.; Smith, Matthew
- Global Change Biology, Vol. 21, Issue 5
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014
- Love, Michael I.; Huber, Wolfgang; Anders, Simon
- Genome Biology, Vol. 15, Issue 12
Qiita: rapid, web-enabled microbiome meta-analysis
journal, October 2018
- Gonzalez, Antonio; Navas-Molina, Jose A.; Kosciolek, Tomasz
- Nature Methods, Vol. 15, Issue 10
Carbon cycle confidence and uncertainty: Exploring variation among soil biogeochemical models
journal, November 2017
- Wieder, William R.; Hartman, Melannie D.; Sulman, Benjamin N.
- Global Change Biology, Vol. 24, Issue 4
Toward an Ecological Classification of soil Bacteria
journal, June 2007
- Fierer, Noah; Bradford, Mark A.; Jackson, Robert B.
- Ecology, Vol. 88, Issue 6