Metabolic Remodeling during Nitrogen Fixation in Zymomonas mobilis
Abstract
Zymomonas mobilis is an ethanologenic bacterium currently being developed for production of advanced biofuels. Recent studies have shown that Z. mobilis can fix dinitrogen gas (N2) as a sole nitrogen source. During N2 fixation, Z. mobilis exhibits increased biomass-specific rates of ethanol production. In order to better understand the physiology of Z. mobilis during N2 fixation and during changes in ammonium (NH4+) availability, we performed liquid chromatography-mass spectrometry (LC-MS)-based targeted metabolomics and shotgun proteomics under three regimes of nitrogen availability: continuous N2 fixation, gradual NH4+ depletion, and acute NH4+ addition to N2-fixing cells. We report dynamic changes in abundance of proteins and metabolites related to nitrogen fixation, motility, ammonium assimilation, amino acid biosynthesis, nucleotide biosynthesis, isoprenoid biosynthesis, and Entner-Doudoroff (ED) glycolysis, providing insight into the regulatory mechanisms that control these processes in Z. mobilis. Our analysis identified potential physiological mechanisms that may contribute to increased specific ethanol production during N2 fixation, including decreased activity of biosynthetic pathways, increased protein abundance of alcohol dehydrogenase (ADHI), and increased thermodynamic favorability of the ED pathway. Of particular relevance to advanced biofuel production, we found that intermediates in the methylerythritol phosphate (MEP) pathway for isoprenoid biosynthesis were depleted during N2 fixation, coinciding with decreasedmore »
- Authors:
-
[1];
[2];
[1];
[3];
[4];
[1];
- DOE Great Lakes Bioenergy Research Center, University of Wisconsin–Madison, Madison, Wisconsin, USA, Department of Bacteriology, University of Wisconsin–Madison, Madison, Wisconsin, USA
- Department of Chemistry, University of Wisconsin–Madison, Madison, Wisconsin, USA, National Center for Quantitative Biology of Complex Systems, University of Wisconsin–Madison, Madison, Wisconsin, USA
- DOE Great Lakes Bioenergy Research Center, University of Wisconsin–Madison, Madison, Wisconsin, USA, Department of Chemistry, University of Wisconsin–Madison, Madison, Wisconsin, USA
- DOE Great Lakes Bioenergy Research Center, University of Wisconsin–Madison, Madison, Wisconsin, USA, Department of Chemistry, University of Wisconsin–Madison, Madison, Wisconsin, USA, National Center for Quantitative Biology of Complex Systems, University of Wisconsin–Madison, Madison, Wisconsin, USA, Department of Biomolecular Chemistry, University of Wisconsin–Madison, Madison, Wisconsin, USA, Morgridge Institute for Research, Madison, Wisconsin, USA
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1830455
- Alternate Identifier(s):
- OSTI ID: 1904707
- Grant/Contract Number:
- SC0018409; FC02-07ER64494; SC0018998
- Resource Type:
- Published Article
- Journal Name:
- mSystems
- Additional Journal Information:
- Journal Name: mSystems Journal Volume: 6 Journal Issue: 6; Journal ID: ISSN 2379-5077
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; MEP pathway; Zymomonas mobilis; biofuels; isoprenoids; metabolomics; nitrogen fixation; nitrogen metabolism; proteomics; systems biology; thermodynamics
Citation Formats
Martien, Julia I., Trujillo, Edna A., Jacobson, Tyler B., Tatli, Mehmet, Hebert, Alexander S., Stevenson, David M., Coon, Joshua J., Amador-Noguez, Daniel, and Vickers, ed., Claudia. Metabolic Remodeling during Nitrogen Fixation in Zymomonas mobilis. United States: N. p., 2021.
Web. doi:10.1128/mSystems.00987-21.
Martien, Julia I., Trujillo, Edna A., Jacobson, Tyler B., Tatli, Mehmet, Hebert, Alexander S., Stevenson, David M., Coon, Joshua J., Amador-Noguez, Daniel, & Vickers, ed., Claudia. Metabolic Remodeling during Nitrogen Fixation in Zymomonas mobilis. United States. https://doi.org/10.1128/mSystems.00987-21
Martien, Julia I., Trujillo, Edna A., Jacobson, Tyler B., Tatli, Mehmet, Hebert, Alexander S., Stevenson, David M., Coon, Joshua J., Amador-Noguez, Daniel, and Vickers, ed., Claudia. Tue .
"Metabolic Remodeling during Nitrogen Fixation in Zymomonas mobilis". United States. https://doi.org/10.1128/mSystems.00987-21.
@article{osti_1830455,
title = {Metabolic Remodeling during Nitrogen Fixation in Zymomonas mobilis},
author = {Martien, Julia I. and Trujillo, Edna A. and Jacobson, Tyler B. and Tatli, Mehmet and Hebert, Alexander S. and Stevenson, David M. and Coon, Joshua J. and Amador-Noguez, Daniel and Vickers, ed., Claudia},
abstractNote = {Zymomonas mobilis is an ethanologenic bacterium currently being developed for production of advanced biofuels. Recent studies have shown that Z. mobilis can fix dinitrogen gas (N2) as a sole nitrogen source. During N2 fixation, Z. mobilis exhibits increased biomass-specific rates of ethanol production. In order to better understand the physiology of Z. mobilis during N2 fixation and during changes in ammonium (NH4+) availability, we performed liquid chromatography-mass spectrometry (LC-MS)-based targeted metabolomics and shotgun proteomics under three regimes of nitrogen availability: continuous N2 fixation, gradual NH4+ depletion, and acute NH4+ addition to N2-fixing cells. We report dynamic changes in abundance of proteins and metabolites related to nitrogen fixation, motility, ammonium assimilation, amino acid biosynthesis, nucleotide biosynthesis, isoprenoid biosynthesis, and Entner-Doudoroff (ED) glycolysis, providing insight into the regulatory mechanisms that control these processes in Z. mobilis. Our analysis identified potential physiological mechanisms that may contribute to increased specific ethanol production during N2 fixation, including decreased activity of biosynthetic pathways, increased protein abundance of alcohol dehydrogenase (ADHI), and increased thermodynamic favorability of the ED pathway. Of particular relevance to advanced biofuel production, we found that intermediates in the methylerythritol phosphate (MEP) pathway for isoprenoid biosynthesis were depleted during N2 fixation, coinciding with decreased protein abundance of deoxyxylulose 5-phosphate synthase (DXS), the first enzyme in the pathway. This implies that DXS protein abundance serves as a native control point in regulating MEP pathway activity in Z. mobilis. The results of this study will inform metabolic engineering to further develop Z. mobilis as a platform organism for biofuel production.},
doi = {10.1128/mSystems.00987-21},
journal = {mSystems},
number = 6,
volume = 6,
place = {United States},
year = {Tue Dec 21 00:00:00 EST 2021},
month = {Tue Dec 21 00:00:00 EST 2021}
}
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