Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants
Abstract
Abstract Green fluorescent protein (GFP) has been widely used for monitoring gene expression and protein localization in diverse organisms. However, highly sensitive imaging equipment, like fluorescence microscope, is usually required for the visualization of GFP, limitings its application to fixed locations in samples. A reporter that can be visualized in real-time regardless the shape, size and location of the target samples will increase the flexibility and efficiency of research work. Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species ( Arabidopsis and tobacco) and two woody plant species (poplar and citrus). We observed bright fluorescence under UV light in all of the four plant species without any effects on plant growth or development. eYGFPuv was shown to be effective for imaging transient expression in leaf and root tissues. With a focus on in vitro transformation, we demonstrated that the transgenic events expressing 1x eYGFPuv could be easily identified visually during the callus stage and the shoot stage, enabling early and efficient selection of transformants. Furthermore, whole-plant level visualization of eYGFPuv revealed its ubiquitous stability in transgenic plants. In addition, our transformation experiments showed that eYGFPuv canmore »
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1828414
- Alternate Identifier(s):
- OSTI ID: 1831670
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- Horticulture Research
- Additional Journal Information:
- Journal Name: Horticulture Research Journal Volume: 8 Journal Issue: 1; Journal ID: ISSN 2662-6810
- Publisher:
- Oxford University Press
- Country of Publication:
- China
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Yuan, Guoliang, Lu, Haiwei, Tang, Dan, Hassan, Md Mahmudul, Li, Yi, Chen, Jin-Gui, Tuskan, Gerald A., and Yang, Xiaohan. Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants. China: N. p., 2021.
Web. doi:10.1038/s41438-021-00663-3.
Yuan, Guoliang, Lu, Haiwei, Tang, Dan, Hassan, Md Mahmudul, Li, Yi, Chen, Jin-Gui, Tuskan, Gerald A., & Yang, Xiaohan. Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants. China. https://doi.org/10.1038/s41438-021-00663-3
Yuan, Guoliang, Lu, Haiwei, Tang, Dan, Hassan, Md Mahmudul, Li, Yi, Chen, Jin-Gui, Tuskan, Gerald A., and Yang, Xiaohan. Mon .
"Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants". China. https://doi.org/10.1038/s41438-021-00663-3.
@article{osti_1828414,
title = {Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants},
author = {Yuan, Guoliang and Lu, Haiwei and Tang, Dan and Hassan, Md Mahmudul and Li, Yi and Chen, Jin-Gui and Tuskan, Gerald A. and Yang, Xiaohan},
abstractNote = {Abstract Green fluorescent protein (GFP) has been widely used for monitoring gene expression and protein localization in diverse organisms. However, highly sensitive imaging equipment, like fluorescence microscope, is usually required for the visualization of GFP, limitings its application to fixed locations in samples. A reporter that can be visualized in real-time regardless the shape, size and location of the target samples will increase the flexibility and efficiency of research work. Here, we report the application of a GFP-like protein, called eYGFPuv, in both transient expression and stable transformation, in two herbaceous plant species ( Arabidopsis and tobacco) and two woody plant species (poplar and citrus). We observed bright fluorescence under UV light in all of the four plant species without any effects on plant growth or development. eYGFPuv was shown to be effective for imaging transient expression in leaf and root tissues. With a focus on in vitro transformation, we demonstrated that the transgenic events expressing 1x eYGFPuv could be easily identified visually during the callus stage and the shoot stage, enabling early and efficient selection of transformants. Furthermore, whole-plant level visualization of eYGFPuv revealed its ubiquitous stability in transgenic plants. In addition, our transformation experiments showed that eYGFPuv can also be used to select transgenic plants without antibiotics. This work demonstrates the feasibility of utilizing 1x eYGFPuv in studies of gene expression and plant transformation in diverse plants.},
doi = {10.1038/s41438-021-00663-3},
journal = {Horticulture Research},
number = 1,
volume = 8,
place = {China},
year = {2021},
month = {11}
}
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