Immobilization of active ammonia-oxidizing archaea in hydrogel beads

Landreau, Matthieu; You, HeeJun; Stahl, David A.; Winkler, Mari K. H.

doi:10.1038/s41545-021-00134-1

Title: Immobilization of active ammonia-oxidizing archaea in hydrogel beads

Abstract

Abstract Ammonia-oxidizing archaea (AOA) are major players in the nitrogen cycle but their cultivation represents a major challenge due to their slow growth rate and limited tendency to form biofilms. In this study, AOA was embedded in small (~2.5 mm) and large (~4.7 mm) poly(vinyl alcohol) (PVA)—sodium alginate (SA) hydrogel beads cross-linked with four agents (calcium, barium, light, or sulfate) to compare the differences in activity, the diffusivity of nitrogen species (NH ₄ ⁺ , NO ₂ ⁻ , and NO ₃ ⁻ ), and polymer leakage in batch systems over time. Sulfate-bound PVA-SA beads were the most stable, releasing the lowest amount of polymer without shrinking. Diffusion coefficients were found to be 2 to 3 times higher in hydrogels than in granules, with ammonium diffusivity being ca . 35% greater than nitrite and nitrate. Despite a longer lag phase in small beads, embedded AOA sustained a high per volume rate of ammonia oxidation compatible with applications in research and wastewater treatment.

Authors:

; You, HeeJun; Stahl, David A.; Winkler, Mari K. H.

Publication Date:: Thu Sep 09 00:00:00 EDT 2021

Research Org.:: Univ. of Washington, Seattle, WA (United States)

Sponsoring Org.:: USDOE Office of Science (SC); Defense Advanced Research Projects Agency (DARPA)

OSTI Identifier:: 1819374

Alternate Identifier(s):: OSTI ID: 1853605

Grant/Contract Number:: SC0020356; HR0011-17-2-0064

Resource Type:: Published Article

Journal Name:: npj Clean Water

Additional Journal Information:: Journal Name: npj Clean Water Journal Volume: 4 Journal Issue: 1; Journal ID: ISSN 2059-7037

Publisher:: Nature Publishing Group

Country of Publication:: United Kingdom

Language:: English

Subject:: 42 ENGINEERING; 54 ENVIRONMENTAL SCIENCES; Engineering; Environmental Sciences & Ecology; Water resources; Environmental monitoring; Pollution remediation

Citation Formats


                    Landreau, Matthieu, You, HeeJun, Stahl, David A., and Winkler, Mari K. H. Immobilization of active ammonia-oxidizing archaea in hydrogel beads.  United Kingdom: N. p., 2021. 
Web.  doi:10.1038/s41545-021-00134-1.

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                    Landreau, Matthieu, You, HeeJun, Stahl, David A., & Winkler, Mari K. H. Immobilization of active ammonia-oxidizing archaea in hydrogel beads.  United Kingdom.  https://doi.org/10.1038/s41545-021-00134-1

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                    Landreau, Matthieu, You, HeeJun, Stahl, David A., and Winkler, Mari K. H. Thu .  
"Immobilization of active ammonia-oxidizing archaea in hydrogel beads".  United Kingdom.  https://doi.org/10.1038/s41545-021-00134-1.

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@article{osti_1819374,

  title        = {Immobilization of active ammonia-oxidizing archaea in hydrogel beads},

  author       = {Landreau, Matthieu and You, HeeJun and Stahl, David A. and Winkler, Mari K. H.},

  abstractNote = {Abstract Ammonia-oxidizing archaea (AOA) are major players in the nitrogen cycle but their cultivation represents a major challenge due to their slow growth rate and limited tendency to form biofilms. In this study, AOA was embedded in small (~2.5 mm) and large (~4.7 mm) poly(vinyl alcohol) (PVA)—sodium alginate (SA) hydrogel beads cross-linked with four agents (calcium, barium, light, or sulfate) to compare the differences in activity, the diffusivity of nitrogen species (NH 4 + , NO 2 − , and NO 3 − ), and polymer leakage in batch systems over time. Sulfate-bound PVA-SA beads were the most stable, releasing the lowest amount of polymer without shrinking. Diffusion coefficients were found to be 2 to 3 times higher in hydrogels than in granules, with ammonium diffusivity being ca . 35% greater than nitrite and nitrate. Despite a longer lag phase in small beads, embedded AOA sustained a high per volume rate of ammonia oxidation compatible with applications in research and wastewater treatment.},

  doi          = {10.1038/s41545-021-00134-1},

  journal      = {npj Clean Water},

  number       = 1,

  volume       = 4,

  place        = {United Kingdom},

  year         = {Thu Sep 09 00:00:00 EDT 2021},

  month        = {Thu Sep 09 00:00:00 EDT 2021}

}

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Works referenced in this record:

Effects of inoculum type and bulk dissolved oxygen concentration on achieving partial nitrification by entrapped-cell-based reactors
journal, July 2014

Rongsayamanont, Chaiwat; Limpiyakorn, Tawan; Khan, Eakalak
Bioresource Technology, Vol. 164
DOI: 10.1016/j.biortech.2014.04.094

The importance of bead size measurement in mass-transfer modelling with immobilised cells
journal, July 1995

Doherty Speirs, E.; Halling, P. J.; McNeil, B.
Applied Microbiology and Biotechnology, Vol. 43, Issue 3
DOI: 10.1007/BF00218446

The immobilization of whole cells: Engineering principles
journal, January 1985

Karel, Steven F.; Libicki, Shari B.; Robertson, Channing R.
Chemical Engineering Science, Vol. 40, Issue 8
DOI: 10.1016/0009-2509(85)80074-9

Immobilization system of Kluyveromyces marxianus cells in barium alginate for inulin hydrolysis
journal, December 2001

Barranco-Florido, Esteban; Garcı́a-Garibay, Mariano; Gómez-Ruiz, Lorena
Process Biochemistry, Vol. 37, Issue 5
DOI: 10.1016/S0032-9592(01)00235-7

Segregation of Biomass in Cyclic Anaerobic/Aerobic Granular Sludge Allows the Enrichment of Anaerobic Ammonium Oxidizing Bacteria at Low Temperatures
journal, September 2011

Winkler, Mari K. H.; Kleerebezem, Robbert; Kuenen, J. Gijs
Environmental Science & Technology, Vol. 45, Issue 17
DOI: 10.1021/es201388t

Performance of the diffusive gradients in thin films technique for measurement of trace metals in low ionic strength freshwaters
journal, February 2003

Peters, Adam J.; Zhang, Hao; Davison, William
Analytica Chimica Acta, Vol. 478, Issue 2
DOI: 10.1016/S0003-2670(02)01512-X

Effect of carrageenan immobilization on the physiological activities of Chlorella vulgaris
journal, February 1998

Lau, P. S.; Tam, N. F. Y.; Wong, Y. S.
Bioresource Technology, Vol. 63, Issue 2
DOI: 10.1016/S0960-8524(97)00111-9

Ecophysiological Characterization of Ammonia-Oxidizing Archaea and Bacteria from Freshwater
journal, June 2012

French, Elizabeth; Kozlowski, Jessica A.; Mukherjee, Maitreyee
Applied and Environmental Microbiology, Vol. 78, Issue 16
DOI: 10.1128/AEM.00432-12

Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol
journal, March 2008

de la Torre, José R.; Walker, Christopher B.; Ingalls, Anitra E.
Environmental Microbiology, Vol. 10, Issue 3
DOI: 10.1111/j.1462-2920.2007.01506.x

Comparative study of the nitrification characteristics of two different nitrifier immobilization methods
journal, June 2009

Li, Zhirong; Zhang, Zhi; Li, Jun
Biodegradation, Vol. 20, Issue 6
DOI: 10.1007/s10532-009-9273-z

Optimization of Entrapping Conditions of Nitrifying Bacteria and Selection of Entrapping Agent
journal, January 2011

Sun, Jingmei; Liu, Jingjing; Liu, Yiman
Procedia Environmental Sciences, Vol. 8
DOI: 10.1016/j.proenv.2011.10.027

Mixed photo-cross-linked polyvinyl alcohol and calcium-alginate gels for cell entrapment
journal, April 1995

Hertzberg, S.; Moen, E.; Vogelsang, C.
Applied Microbiology and Biotechnology, Vol. 43, Issue 1
DOI: 10.1007/BF00170615

Immobilization of activated sludge using improved polyvinyl alcohol (PVA) gel
journal, January 2007

Zhang, Li-sheng; Wu, Wei-zhong; Wang, Jian-long
Journal of Environmental Sciences, Vol. 19, Issue 11
DOI: 10.1016/S1001-0742(07)60211-3

Review: Hydrogels for cell immobilization
journal, May 1996

Jen, Anna C.; Wake, M. Conley; Mikos, Antonios G.
Biotechnology and Bioengineering, Vol. 50, Issue 4
DOI: 10.1002/(SICI)1097-0290(19960520)50:4<357::AID-BIT2>3.0.CO;2-K

A Comparative Study of Immobilized Nitrifying and Co-Immobilized Nitrifying and Denitrifying Bacteria for Ammonia Removal from Sludge Digester Supernatant
journal, July 2008

Hill, Christopher B.; Khan, Eakalak
Water, Air, and Soil Pollution, Vol. 195, Issue 1-4
DOI: 10.1007/s11270-008-9724-x

Environmental applications of immobilized microbial cells: A review
journal, February 1996

Cassidy, M. B.; Lee, H.; Trevors, J. T.
Journal of Industrial Microbiology, Vol. 16, Issue 2
DOI: 10.1007/BF01570068

Nitrogen removal and microbial diversity of activated sludge entrapped in modified poly(vinyl alcohol)–sodium alginate gel
journal, November 2017

Xu, Xiaoyi; Lv, Chenpei; You, Xiaolu
International Biodeterioration & Biodegradation, Vol. 125
DOI: 10.1016/j.ibiod.2017.07.014

Mass transfer and kinetic studies under no cell growth conditions in nitrification using alginate gel immobilized Nitrosomonas
journal, March 2005

Benyahia, F.; Polomarkaki, R.
Process Biochemistry, Vol. 40, Issue 3-4
DOI: 10.1016/j.procbio.2004.05.011

Development and modification of PVA–alginate as a suitable immobilization matrix
journal, November 2011

Zain, Nor Azimah Mohd; Suhaimi, Mohd Suardi; Idris, Ani
Process Biochemistry, Vol. 46, Issue 11
DOI: 10.1016/j.procbio.2011.08.010

Nitrification of NH4-N polluted sea water by immobilized acclimated marine nitrifying sludge (AMNS)
journal, January 1993

Furukawa, Kenji; Ike, Akiko; Ryu, Soo-Lim
Journal of Fermentation and Bioengineering, Vol. 76, Issue 6
DOI: 10.1016/0922-338X(93)90251-3

Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria
journal, September 2009

Martens-Habbena, Willm; Berube, Paul M.; Urakawa, Hidetoshi
Nature, Vol. 461, Issue 7266
DOI: 10.1038/nature08465

Functional stability of temperature-compensated nitrification in domestic wastewater treatment obtained with PVA-SbQ/alginate gel entrapment
journal, July 1997

Vogelsang, Christian; Husby, Asbjørn; Østgaard, Kjetill
Water Research, Vol. 31, Issue 7
DOI: 10.1016/S0043-1354(97)00009-2

Simple, rapid and effective preservation and reactivation of anaerobic ammonium oxidizing bacterium “Candidatus Brocadia sinica”
journal, June 2014

Ali, Muhammad; Oshiki, Mamoru; Okabe, Satoshi
Water Research, Vol. 57
DOI: 10.1016/j.watres.2014.03.036

Reactive toughening of polyvinyl alcohol hydrogel and its wastewater treatment performance by immobilization of microorganisms
journal, January 2015

Zhang, Yi; Hui, Bing; Ye, Lin
RSC Advances, Vol. 5, Issue 111
DOI: 10.1039/C5RA20495J

Diffusion and Partitioning of Solutes in Agarose Hydrogels: The Relative Influence of Electrostatic and Specific Interactions
journal, November 2003

Fatin-Rouge, Nicolas; Milon, Antoine; Buffle, Jacques
The Journal of Physical Chemistry B, Vol. 107, Issue 44
DOI: 10.1021/jp0303164

Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate
journal, July 2017

Dong, Yuwei; Zhang, Yanqiu; Tu, Baojun
Brazilian Journal of Microbiology, Vol. 48, Issue 3
DOI: 10.1016/j.bjm.2017.02.001

Immobilization of Infant Fecal Microbiota and Utilization in an in vitro Colonic Fermentation Model
journal, April 2004

Cinquin, C.; Le Blay, G.; Fliss, I.
Microbial Ecology, Vol. 48, Issue 1
DOI: 10.1007/s00248-003-2022-7

Immobilization of activated sludge in poly(ethylene glycol) by UV technology and its application in micro-polluted wastewater
journal, June 2010

Xiangli, Qiao; Zhe, Liu; Zhiwei, Liu
Biochemical Engineering Journal, Vol. 50, Issue 1-2
DOI: 10.1016/j.bej.2010.03.007

Immobilization of activated sludge by PVA-boric acid method
journal, July 1987

Hashimoto, Susumu; Furukawa, Kenji
Biotechnology and Bioengineering, Vol. 30, Issue 1
DOI: 10.1002/bit.260300108

Cultivation of an obligate acidophilic ammonia oxidizer from a nitrifying acid soil
journal, September 2011

Lehtovirta-Morley, L. E.; Stoecker, K.; Vilcinskas, A.
Proceedings of the National Academy of Sciences, Vol. 108, Issue 38
DOI: 10.1073/pnas.1107196108

Optimization of the mechanical strength of PVA/alginate gel beads and their effects on the ammonia-oxidizing activity
journal, June 2014

Bae, Hyokwan; Yang, Heejeong; Choi, Minkyu
Desalination and Water Treatment, Vol. 53, Issue 9
DOI: 10.1080/19443994.2014.927118

Key Physiology of Anaerobic Ammonium Oxidation
journal, July 1999

Strous, Marc; Kuenen, J. Gijs; Jetten, Mike S. M.
Applied and Environmental Microbiology, Vol. 65, Issue 7
DOI: 10.1128/AEM.65.7.3248-3250.1999

Bioremediation of diesel-contaminated soil by microorganisms immobilised in polyvinyl alcohol
journal, September 2004

Cunningham, C. J.; Ivshina, I. B.; Lozinsky, V. I.
International Biodeterioration & Biodegradation, Vol. 54, Issue 2-3
DOI: 10.1016/j.ibiod.2004.03.005

Characterization of nitrifying and denitrifying bacteria coimmobilized in PVA and kinetics model of biological nitrogen removal by coimmobilized cells
journal, January 2002

Cao, Guo-min; Zhao, Qing-xiang; Sun, Xian-bo
Enzyme and Microbial Technology, Vol. 30, Issue 1
DOI: 10.1016/S0141-0229(01)00458-6

Occurrence of Ammonia-Oxidizing Archaea in Wastewater Treatment Plant Bioreactors
journal, August 2006

Park, Hee-Deung; Wells, George F.; Bae, Hyokwan
Applied and Environmental Microbiology, Vol. 72, Issue 8
DOI: 10.1128/AEM.00402-06

Electrostatic droplet generation: Mechanism of polymer droplet formation
journal, June 1994

Bugarski, Branko; Li, Qiangliang; Goosen, Mattheus F. A.
AIChE Journal, Vol. 40, Issue 6
DOI: 10.1002/aic.690400613

Selective sludge removal in a segregated aerobic granular biomass system as a strategy to control PAO–GAO competition at high temperatures
journal, May 2011

Winkler, M. -K. H.; Bassin, J. P.; Kleerebezem, R.
Water Research, Vol. 45, Issue 11
DOI: 10.1016/j.watres.2011.03.024

Rapid and successful start-up of anammox process by immobilizing the minimal quantity of biomass in PVA-SA gel beads
journal, August 2015

Ali, Muhammad; Oshiki, Mamoru; Rathnayake, Lashitha
Water Research, Vol. 79
DOI: 10.1016/j.watres.2015.04.024

Entrapment of anaerobic thermophilic and hyperthermophilic marine micro-organisms in a gellan/xanthan matrix
journal, April 2016

Landreau, M.; Duthoit, F.; Claeys-Bruno, M.
Journal of Applied Microbiology, Vol. 120, Issue 6
DOI: 10.1111/jam.13118

Novel single-stage autotrophic nitrogen removal via co-immobilizing partial nitrifying and anammox biomass
journal, August 2013

Qiao, Sen; Tian, Tian; Duan, Xiumei
Chemical Engineering Journal, Vol. 230
DOI: 10.1016/j.cej.2013.06.048

Alginate: Properties and biomedical applications
journal, January 2012

Lee, Kuen Yong; Mooney, David J.
Progress in Polymer Science, Vol. 37, Issue 1
DOI: 10.1016/j.progpolymsci.2011.06.003

Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA-immobilized cell beads
journal, July 2003

Chen, Kuo-Cheng; Wu, Jane-Yii; Yang, Wen-Bin
Biotechnology and Bioengineering, Vol. 83, Issue 7
DOI: 10.1002/bit.10730

Cell immobilization using PVA crosslinked with boric acid
journal, February 1992

Wu, Kuo-Ying Amanda; Wisecarver, Keith D.
Biotechnology and Bioengineering, Vol. 39, Issue 4
DOI: 10.1002/bit.260390411

A convenient photochemical method to immobilize enzymes
journal, November 1984

Ichimura, Kunihiro
Journal of Polymer Science: Polymer Chemistry Edition, Vol. 22, Issue 11
DOI: 10.1002/pol.1984.170221108

Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments
journal, August 1998

Lozinsky, V. I.; Plieva, F. M.
Enzyme and Microbial Technology, Vol. 23, Issue 3-4
DOI: 10.1016/S0141-0229(98)00036-2

Microencapsulation of microbial cells
journal, May 2013

Rathore, Sweta; Desai, Parind Mahendrakumar; Liew, Celine Valeria
Journal of Food Engineering, Vol. 116, Issue 2
DOI: 10.1016/j.jfoodeng.2012.12.022

Effects of Polymer Concentration and Cross-Linking Density on Rheology of Chemically Cross-Linked Poly(vinyl alcohol) near the Gelation Threshold
journal, January 1996

Kjøniksen, Anna-Lena; Nyström, Bo
Macromolecules, Vol. 29, Issue 15
DOI: 10.1021/ma960094q

Completely autotrophic nitrogen removal over nitrite in one single reactor
journal, May 2002

Sliekers, A.
Water Research, Vol. 36, Issue 10
DOI: 10.1016/S0043-1354(01)00476-6

Immobilization of nitrifying bacteria in porous pellets of urethane gel for removal of ammonium nitrogen from waste-water
journal, January 1992

Sumino, T.; Nakamura, H.; Mori, N.
Applied Microbiology and Biotechnology, Vol. 36, Issue 4
DOI: 10.1007/BF00170203

Behaviour of Saccharomyces cerevisiae cells entrapped in a polyacrylamide gel and performing alcoholic fermentation
journal, January 1981

Siess, M. H.; Divies, C.
European Journal of Applied Microbiology and Biotechnology, Vol. 12, Issue 1
DOI: 10.1007/BF00508112

Novel crosslinking methods to design hydrogels
journal, December 2012

Hennink, W. E.; van Nostrum, C. F.
Advanced Drug Delivery Reviews, Vol. 64
DOI: 10.1016/j.addr.2012.09.009

Ammonia-Oxidising Archaea – Physiology, Ecology and Evolution
book, November 2010

Schleper, Christa; Nicol, Graeme W.
Advances in Microbial Physiology
DOI: 10.1016/B978-0-12-381045-8.00001-1

Preparation of crosslinked macroporous PVA foam carrier for immobilization of microorganisms
journal, January 2010

Bai, Xue; Ye, Zheng-fang; Li, Yan-feng
Process Biochemistry, Vol. 45, Issue 1
DOI: 10.1016/j.procbio.2009.08.003

Anammox brings WWTP closer to energy autarky due to increased biogas production and reduced aeration energy for N-removal
journal, February 2008

Siegrist, H.; Salzgeber, D.; Eugster, J.
Water Science and Technology, Vol. 57, Issue 3
DOI: 10.2166/wst.2008.048

Complete autotrophic denitrification in a single reactor using nitritation and anammox gel carriers
journal, November 2013

Isaka, Kazuichi; Kimura, Yuya; Yamamoto, Tomoko
Bioresource Technology, Vol. 147
DOI: 10.1016/j.biortech.2013.07.095

Nitrogen removal in seawater using nitrifying and denitrifying bacteria immobilized in porous cellulose carrier
journal, October 1996

Sakairi, Maria Antonina Catalan; Yasuda, Kimiaki; Matsumura, Masatoshi
Water Science and Technology, Vol. 34, Issue 7-8
DOI: 10.2166/wst.1996.0631

Preparation of PVA hydrogel beads and adsorption mechanism for advanced phosphate removal
journal, January 2014

Hui, Bing; Zhang, Yi; Ye, Lin
Chemical Engineering Journal, Vol. 235
DOI: 10.1016/j.cej.2013.09.045

Isolation of an autotrophic ammonia-oxidizing marine archaeon
journal, September 2005

Könneke, Martin; Bernhard, Anne E.; de la Torre, José R.
Nature, Vol. 437, Issue 7058
DOI: 10.1038/nature03911

Immobilized yeast cell systems for continuous fermentation applications
journal, August 2006

Verbelen, Pieter J.; De Schutter, David P.; Delvaux, Filip
Biotechnology Letters, Vol. 28, Issue 19
DOI: 10.1007/s10529-006-9132-5

Mass transfer effect and intermediate detection for phenol degradation in immobilized Pseudomonas putida systems
journal, May 2003

Chung, Tsuey-Ping; Tseng, Hsiu-Ya; Juang, Ruey-Shin
Process Biochemistry, Vol. 38, Issue 10
DOI: 10.1016/S0032-9592(03)00038-4

Preparation of Encapsulated Microbial Cells for Environmental Applications
journal, February 1992

Stormo, Keith E.; Crawford, Ronald L.
Applied and Environmental Microbiology, Vol. 58, Issue 2
DOI: 10.1128/aem.58.2.727-730.1992

Effect of temperature and pH value on the growth-rate constants of nitrifying bacteria in the activated-sludge process
journal, January 1983

Painter, H.; Loveless, J.
Water Research, Vol. 17, Issue 3
DOI: 10.1016/0043-1354(83)90176-8

Immobilization of Baker's yeast invertase in PVA–alginate matrix using innovative immobilization technique
journal, April 2008

Idris, Ani; Zain, Nor Azimah Mohd; Suhaimi, Mohd Suardi
Process Biochemistry, Vol. 43, Issue 4
DOI: 10.1016/j.procbio.2007.12.008

Fabrication of poly(vinyl alcohol) hydrogel beads crosslinked using sodium sulfate for microorganism immobilization
journal, February 2011

Takei, Takayuki; Ikeda, Kaoru; Ijima, Hiroyuki
Process Biochemistry, Vol. 46, Issue 2
DOI: 10.1016/j.procbio.2010.10.011

Degradation of phenol by PAA-immobilized Candida tropicalis
journal, September 2002

Chen, Kuo-Cheng; Lin, Yun-Huin; Chen, Wen-Hsiang
Enzyme and Microbial Technology, Vol. 31, Issue 4
DOI: 10.1016/S0141-0229(02)00148-5

Optimization of microencapsulation parameters: Semipermeable microcapsules as a bioartificial pancreas
journal, February 1985

Goosen, Mattheus F. A.; O'Shea, Geraldine M.; Gharapetian, Hrire M.
Biotechnology and Bioengineering, Vol. 27, Issue 2
DOI: 10.1002/bit.260270207

Immobilization of microorganisms with phosphorylated polyvinyl alcohol (PVA) gel
journal, January 1994

Chen, Kuo-Cheng; Lin, Ying-Feng
Enzyme and Microbial Technology, Vol. 16, Issue 1
DOI: 10.1016/0141-0229(94)90113-9

Comparison of the effects of different salts on aerobic ammonia oxidizers for treating ammonium-rich organic wastewater by free and sodium alginate immobilized biomass system
journal, October 2010

Yan, Jia; Jetten, Mike; Rang, Jinlong
Chemosphere, Vol. 81, Issue 5
DOI: 10.1016/j.chemosphere.2010.03.025

Core-shell structured poly(vinyl alcohol)/sodium alginate bead for single-stage autotrophic nitrogen removal
journal, August 2017

Bae, Hyokwan; Choi, Minkyu; Chung, Yun-Chul
Chemical Engineering Journal, Vol. 322
DOI: 10.1016/j.cej.2017.03.119

First full-scale nitritation-anammox plant using gel entrapment technology for ammonia plant effluent
journal, June 2017

Isaka, Kazuichi; Kimura, Yuya; Matsuura, Masahiro
Biochemical Engineering Journal, Vol. 122
DOI: 10.1016/j.bej.2017.03.005

Viscosity of moderately concentrated aqueous solutions of polyvinyl alcohol
journal, June 1962

Ōyanagi, Yasuji; Matsumoto, Masakazu
Journal of Colloid Science, Vol. 17, Issue 5
DOI: 10.1016/0095-8522(62)90054-5

Influence of Process Conditions During Impulsed Electrostatic Droplet Formation on Size Distribution of Hydrogel Beads
journal, January 2004

Lewińska, Dorota; Rosiński, Stefan; Weryński, Andrzej
Artificial Cells, Blood Substitutes, and Biotechnology, Vol. 32, Issue 1
DOI: 10.1081/BIO-120028667

Physiology and Diversity of Ammonia-Oxidizing Archaea
journal, October 2012

Stahl, David A.; de la Torre, José R.
Annual Review of Microbiology, Vol. 66, Issue 1
DOI: 10.1146/annurev-micro-092611-150128

Alginate as immobilization matrix for cells
journal, January 1990

Smidsrod, O.; Skjakbrk, G.
Trends in Biotechnology, Vol. 8
DOI: 10.1016/0167-7799(90)90139-O

Occurrence of ammonia-oxidizing Archaea in activated sludges of a laboratory scale reactor and two wastewater treatment plants
journal, September 2009

Zhang, T.; Jin, T.; Yan, Q.
Journal of Applied Microbiology, Vol. 107, Issue 3
DOI: 10.1111/j.1365-2672.2009.04283.x

Reject water treatment by improvement of whole cell anammox entrapment using polyvinyl alcohol/alginate gel
journal, April 2011

Quan, Lai Minh; Khanh, Do Phuong; Hira, Daisuke
Biodegradation, Vol. 22, Issue 6
DOI: 10.1007/s10532-011-9471-3

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Preparation of pH ‐responsive cellulose nanofibril/sodium alginate based hydrogels for drug release

Journal Article Li, Yuhang ; Wang, Cong ; Luan, Yunhao ; ... - Journal of Applied Polymer Science

Abstract In this work, hydrogels composed of cellulose nanofibril (CNF) and sodium alginate (SA) were prepared as a pH‐responsive release system by controlling the amount of CNF and SA. Scanning electron microscopy, thermogravimetric analysis, and swelling rate methods were used to characterize. The results showed that when the mass ratio of CNF to SA was 2:1, CNF/SA hydrogels with better morphology were obtained. Due to the deprotonation or protonation of calcium alginate, as the pH changing from 1.5 to 11.0, the swelling ratio increased from 7 times to 19 times compared with its dry weight. The drug release behaviors ofmore »« less
https://doi.org/10.1002/app.51647
Genomics insights into ecotype formation of ammonia‐oxidizing archaea in the deep ocean

Journal Article Wang, Yong ; Huang, Jiao‐Mei ; Cui, Guo‐Jie ; ... - Environmental Microbiology

Summary Various lineages of ammonia‐oxidizing archaea (AOA) are present in deep waters, but the mechanisms that determine ecotype formation are obscure. We studied 18 high‐quality genomes of the marine group I AOA lineages (alpha, gamma and delta) from the Mariana and Ogasawara trenches. The genomes of alpha AOA resembled each other, while those of gamma and delta lineages were more divergent and had even undergone insertion of some phage genes. The instability of the gamma and delta AOA genomes could be partially due to the loss of DNA polymerase B ( polB) and methyladenine DNA glycosylase ( tag ) genesmore »« less
Cited by 29
https://doi.org/10.1111/1462-2920.14518

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Title: Immobilization of active ammonia-oxidizing archaea in hydrogel beads

Abstract

Citation Formats

Effects of inoculum type and bulk dissolved oxygen concentration on achieving partial nitrification by entrapped-cell-based reactors journal, July 2014

The importance of bead size measurement in mass-transfer modelling with immobilised cells journal, July 1995

The immobilization of whole cells: Engineering principles journal, January 1985

Immobilization system of Kluyveromyces marxianus cells in barium alginate for inulin hydrolysis journal, December 2001

Segregation of Biomass in Cyclic Anaerobic/Aerobic Granular Sludge Allows the Enrichment of Anaerobic Ammonium Oxidizing Bacteria at Low Temperatures journal, September 2011

Performance of the diffusive gradients in thin films technique for measurement of trace metals in low ionic strength freshwaters journal, February 2003

Effect of carrageenan immobilization on the physiological activities of Chlorella vulgaris journal, February 1998

Ecophysiological Characterization of Ammonia-Oxidizing Archaea and Bacteria from Freshwater journal, June 2012

Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol journal, March 2008

Comparative study of the nitrification characteristics of two different nitrifier immobilization methods journal, June 2009

Optimization of Entrapping Conditions of Nitrifying Bacteria and Selection of Entrapping Agent journal, January 2011

Mixed photo-cross-linked polyvinyl alcohol and calcium-alginate gels for cell entrapment journal, April 1995

Immobilization of activated sludge using improved polyvinyl alcohol (PVA) gel journal, January 2007

Review: Hydrogels for cell immobilization journal, May 1996

A Comparative Study of Immobilized Nitrifying and Co-Immobilized Nitrifying and Denitrifying Bacteria for Ammonia Removal from Sludge Digester Supernatant journal, July 2008

Environmental applications of immobilized microbial cells: A review journal, February 1996

Nitrogen removal and microbial diversity of activated sludge entrapped in modified poly(vinyl alcohol)–sodium alginate gel journal, November 2017

Mass transfer and kinetic studies under no cell growth conditions in nitrification using alginate gel immobilized Nitrosomonas journal, March 2005

Development and modification of PVA–alginate as a suitable immobilization matrix journal, November 2011

Nitrification of NH4-N polluted sea water by immobilized acclimated marine nitrifying sludge (AMNS) journal, January 1993

Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria journal, September 2009

Functional stability of temperature-compensated nitrification in domestic wastewater treatment obtained with PVA-SbQ/alginate gel entrapment journal, July 1997

Simple, rapid and effective preservation and reactivation of anaerobic ammonium oxidizing bacterium “Candidatus Brocadia sinica” journal, June 2014

Reactive toughening of polyvinyl alcohol hydrogel and its wastewater treatment performance by immobilization of microorganisms journal, January 2015

Diffusion and Partitioning of Solutes in Agarose Hydrogels: The Relative Influence of Electrostatic and Specific Interactions journal, November 2003

Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate journal, July 2017

Immobilization of Infant Fecal Microbiota and Utilization in an in vitro Colonic Fermentation Model journal, April 2004

Immobilization of activated sludge in poly(ethylene glycol) by UV technology and its application in micro-polluted wastewater journal, June 2010

Immobilization of activated sludge by PVA-boric acid method journal, July 1987

Cultivation of an obligate acidophilic ammonia oxidizer from a nitrifying acid soil journal, September 2011

Optimization of the mechanical strength of PVA/alginate gel beads and their effects on the ammonia-oxidizing activity journal, June 2014

Key Physiology of Anaerobic Ammonium Oxidation journal, July 1999

Bioremediation of diesel-contaminated soil by microorganisms immobilised in polyvinyl alcohol journal, September 2004

Characterization of nitrifying and denitrifying bacteria coimmobilized in PVA and kinetics model of biological nitrogen removal by coimmobilized cells journal, January 2002

Occurrence of Ammonia-Oxidizing Archaea in Wastewater Treatment Plant Bioreactors journal, August 2006

Electrostatic droplet generation: Mechanism of polymer droplet formation journal, June 1994

Selective sludge removal in a segregated aerobic granular biomass system as a strategy to control PAO–GAO competition at high temperatures journal, May 2011

Rapid and successful start-up of anammox process by immobilizing the minimal quantity of biomass in PVA-SA gel beads journal, August 2015

Entrapment of anaerobic thermophilic and hyperthermophilic marine micro-organisms in a gellan/xanthan matrix journal, April 2016

Novel single-stage autotrophic nitrogen removal via co-immobilizing partial nitrifying and anammox biomass journal, August 2013

Alginate: Properties and biomedical applications journal, January 2012

Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA-immobilized cell beads journal, July 2003

Cell immobilization using PVA crosslinked with boric acid journal, February 1992

A convenient photochemical method to immobilize enzymes journal, November 1984

Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments journal, August 1998

Microencapsulation of microbial cells journal, May 2013

Effects of Polymer Concentration and Cross-Linking Density on Rheology of Chemically Cross-Linked Poly(vinyl alcohol) near the Gelation Threshold journal, January 1996

Completely autotrophic nitrogen removal over nitrite in one single reactor journal, May 2002

Immobilization of nitrifying bacteria in porous pellets of urethane gel for removal of ammonium nitrogen from waste-water journal, January 1992

Behaviour of Saccharomyces cerevisiae cells entrapped in a polyacrylamide gel and performing alcoholic fermentation journal, January 1981

Novel crosslinking methods to design hydrogels journal, December 2012

Ammonia-Oxidising Archaea – Physiology, Ecology and Evolution book, November 2010

Preparation of crosslinked macroporous PVA foam carrier for immobilization of microorganisms journal, January 2010

Anammox brings WWTP closer to energy autarky due to increased biogas production and reduced aeration energy for N-removal journal, February 2008

Complete autotrophic denitrification in a single reactor using nitritation and anammox gel carriers journal, November 2013

Nitrogen removal in seawater using nitrifying and denitrifying bacteria immobilized in porous cellulose carrier journal, October 1996

Preparation of PVA hydrogel beads and adsorption mechanism for advanced phosphate removal journal, January 2014

Isolation of an autotrophic ammonia-oxidizing marine archaeon journal, September 2005

Immobilized yeast cell systems for continuous fermentation applications journal, August 2006

Mass transfer effect and intermediate detection for phenol degradation in immobilized Pseudomonas putida systems journal, May 2003

Preparation of Encapsulated Microbial Cells for Environmental Applications journal, February 1992

Effect of temperature and pH value on the growth-rate constants of nitrifying bacteria in the activated-sludge process journal, January 1983

Immobilization of Baker's yeast invertase in PVA–alginate matrix using innovative immobilization technique journal, April 2008

Fabrication of poly(vinyl alcohol) hydrogel beads crosslinked using sodium sulfate for microorganism immobilization journal, February 2011

Degradation of phenol by PAA-immobilized Candida tropicalis journal, September 2002

Optimization of microencapsulation parameters: Semipermeable microcapsules as a bioartificial pancreas journal, February 1985

Immobilization of microorganisms with phosphorylated polyvinyl alcohol (PVA) gel journal, January 1994

Comparison of the effects of different salts on aerobic ammonia oxidizers for treating ammonium-rich organic wastewater by free and sodium alginate immobilized biomass system journal, October 2010

Core-shell structured poly(vinyl alcohol)/sodium alginate bead for single-stage autotrophic nitrogen removal journal, August 2017

First full-scale nitritation-anammox plant using gel entrapment technology for ammonia plant effluent journal, June 2017

Viscosity of moderately concentrated aqueous solutions of polyvinyl alcohol journal, June 1962

Influence of Process Conditions During Impulsed Electrostatic Droplet Formation on Size Distribution of Hydrogel Beads journal, January 2004

Physiology and Diversity of Ammonia-Oxidizing Archaea journal, October 2012

Alginate as immobilization matrix for cells journal, January 1990

Occurrence of ammonia-oxidizing Archaea in activated sludges of a laboratory scale reactor and two wastewater treatment plants journal, September 2009

Reject water treatment by improvement of whole cell anammox entrapment using polyvinyl alcohol/alginate gel journal, April 2011

Effects of inoculum type and bulk dissolved oxygen concentration on achieving partial nitrification by entrapped-cell-based reactors
journal, July 2014

The importance of bead size measurement in mass-transfer modelling with immobilised cells
journal, July 1995

The immobilization of whole cells: Engineering principles
journal, January 1985

Immobilization system of Kluyveromyces marxianus cells in barium alginate for inulin hydrolysis
journal, December 2001

Segregation of Biomass in Cyclic Anaerobic/Aerobic Granular Sludge Allows the Enrichment of Anaerobic Ammonium Oxidizing Bacteria at Low Temperatures
journal, September 2011

Performance of the diffusive gradients in thin films technique for measurement of trace metals in low ionic strength freshwaters
journal, February 2003

Effect of carrageenan immobilization on the physiological activities of Chlorella vulgaris
journal, February 1998

Ecophysiological Characterization of Ammonia-Oxidizing Archaea and Bacteria from Freshwater
journal, June 2012

Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol
journal, March 2008

Comparative study of the nitrification characteristics of two different nitrifier immobilization methods
journal, June 2009

Optimization of Entrapping Conditions of Nitrifying Bacteria and Selection of Entrapping Agent
journal, January 2011

Mixed photo-cross-linked polyvinyl alcohol and calcium-alginate gels for cell entrapment
journal, April 1995

Immobilization of activated sludge using improved polyvinyl alcohol (PVA) gel
journal, January 2007

Review: Hydrogels for cell immobilization
journal, May 1996

A Comparative Study of Immobilized Nitrifying and Co-Immobilized Nitrifying and Denitrifying Bacteria for Ammonia Removal from Sludge Digester Supernatant
journal, July 2008

Environmental applications of immobilized microbial cells: A review
journal, February 1996

Nitrogen removal and microbial diversity of activated sludge entrapped in modified poly(vinyl alcohol)–sodium alginate gel
journal, November 2017

Mass transfer and kinetic studies under no cell growth conditions in nitrification using alginate gel immobilized Nitrosomonas
journal, March 2005

Development and modification of PVA–alginate as a suitable immobilization matrix
journal, November 2011

Nitrification of NH4-N polluted sea water by immobilized acclimated marine nitrifying sludge (AMNS)
journal, January 1993

Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria
journal, September 2009

Functional stability of temperature-compensated nitrification in domestic wastewater treatment obtained with PVA-SbQ/alginate gel entrapment
journal, July 1997

Simple, rapid and effective preservation and reactivation of anaerobic ammonium oxidizing bacterium “Candidatus Brocadia sinica”
journal, June 2014

Reactive toughening of polyvinyl alcohol hydrogel and its wastewater treatment performance by immobilization of microorganisms
journal, January 2015

Diffusion and Partitioning of Solutes in Agarose Hydrogels: The Relative Influence of Electrostatic and Specific Interactions
journal, November 2003

Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate
journal, July 2017

Immobilization of Infant Fecal Microbiota and Utilization in an in vitro Colonic Fermentation Model
journal, April 2004

Immobilization of activated sludge in poly(ethylene glycol) by UV technology and its application in micro-polluted wastewater
journal, June 2010

Immobilization of activated sludge by PVA-boric acid method
journal, July 1987

Cultivation of an obligate acidophilic ammonia oxidizer from a nitrifying acid soil
journal, September 2011

Optimization of the mechanical strength of PVA/alginate gel beads and their effects on the ammonia-oxidizing activity
journal, June 2014

Key Physiology of Anaerobic Ammonium Oxidation
journal, July 1999

Bioremediation of diesel-contaminated soil by microorganisms immobilised in polyvinyl alcohol
journal, September 2004

Characterization of nitrifying and denitrifying bacteria coimmobilized in PVA and kinetics model of biological nitrogen removal by coimmobilized cells
journal, January 2002

Occurrence of Ammonia-Oxidizing Archaea in Wastewater Treatment Plant Bioreactors
journal, August 2006

Electrostatic droplet generation: Mechanism of polymer droplet formation
journal, June 1994

Selective sludge removal in a segregated aerobic granular biomass system as a strategy to control PAO–GAO competition at high temperatures
journal, May 2011

Rapid and successful start-up of anammox process by immobilizing the minimal quantity of biomass in PVA-SA gel beads
journal, August 2015

Entrapment of anaerobic thermophilic and hyperthermophilic marine micro-organisms in a gellan/xanthan matrix
journal, April 2016

Novel single-stage autotrophic nitrogen removal via co-immobilizing partial nitrifying and anammox biomass
journal, August 2013

Alginate: Properties and biomedical applications
journal, January 2012

Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA-immobilized cell beads
journal, July 2003

Cell immobilization using PVA crosslinked with boric acid
journal, February 1992

A convenient photochemical method to immobilize enzymes
journal, November 1984

Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments
journal, August 1998

Microencapsulation of microbial cells
journal, May 2013

Effects of Polymer Concentration and Cross-Linking Density on Rheology of Chemically Cross-Linked Poly(vinyl alcohol) near the Gelation Threshold
journal, January 1996

Completely autotrophic nitrogen removal over nitrite in one single reactor
journal, May 2002

Immobilization of nitrifying bacteria in porous pellets of urethane gel for removal of ammonium nitrogen from waste-water
journal, January 1992

Behaviour of Saccharomyces cerevisiae cells entrapped in a polyacrylamide gel and performing alcoholic fermentation
journal, January 1981

Novel crosslinking methods to design hydrogels
journal, December 2012

Ammonia-Oxidising Archaea – Physiology, Ecology and Evolution
book, November 2010

Preparation of crosslinked macroporous PVA foam carrier for immobilization of microorganisms
journal, January 2010

Anammox brings WWTP closer to energy autarky due to increased biogas production and reduced aeration energy for N-removal
journal, February 2008

Complete autotrophic denitrification in a single reactor using nitritation and anammox gel carriers
journal, November 2013

Nitrogen removal in seawater using nitrifying and denitrifying bacteria immobilized in porous cellulose carrier
journal, October 1996

Preparation of PVA hydrogel beads and adsorption mechanism for advanced phosphate removal
journal, January 2014

Isolation of an autotrophic ammonia-oxidizing marine archaeon
journal, September 2005

Immobilized yeast cell systems for continuous fermentation applications
journal, August 2006

Mass transfer effect and intermediate detection for phenol degradation in immobilized Pseudomonas putida systems
journal, May 2003

Preparation of Encapsulated Microbial Cells for Environmental Applications
journal, February 1992

Effect of temperature and pH value on the growth-rate constants of nitrifying bacteria in the activated-sludge process
journal, January 1983

Immobilization of Baker's yeast invertase in PVA–alginate matrix using innovative immobilization technique
journal, April 2008

Fabrication of poly(vinyl alcohol) hydrogel beads crosslinked using sodium sulfate for microorganism immobilization
journal, February 2011

Degradation of phenol by PAA-immobilized Candida tropicalis
journal, September 2002

Optimization of microencapsulation parameters: Semipermeable microcapsules as a bioartificial pancreas
journal, February 1985

Immobilization of microorganisms with phosphorylated polyvinyl alcohol (PVA) gel
journal, January 1994

Comparison of the effects of different salts on aerobic ammonia oxidizers for treating ammonium-rich organic wastewater by free and sodium alginate immobilized biomass system
journal, October 2010

Core-shell structured poly(vinyl alcohol)/sodium alginate bead for single-stage autotrophic nitrogen removal
journal, August 2017

First full-scale nitritation-anammox plant using gel entrapment technology for ammonia plant effluent
journal, June 2017

Viscosity of moderately concentrated aqueous solutions of polyvinyl alcohol
journal, June 1962

Influence of Process Conditions During Impulsed Electrostatic Droplet Formation on Size Distribution of Hydrogel Beads
journal, January 2004

Physiology and Diversity of Ammonia-Oxidizing Archaea
journal, October 2012

Alginate as immobilization matrix for cells
journal, January 1990

Occurrence of ammonia-oxidizing Archaea in activated sludges of a laboratory scale reactor and two wastewater treatment plants
journal, September 2009

Reject water treatment by improvement of whole cell anammox entrapment using polyvinyl alcohol/alginate gel
journal, April 2011