In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways

Weis, Simone Nardin; Souza, Jaques Miranda F.; Hoppe, Juliana Bender; Firmino, Marina; Auer, Manfred; Ataii, Nassim N.; da Silva, Leonardo Assis; Gaelzer, Mariana Maier; Klein, Caroline Peres; Mól, Alan R.; de Lima, Consuelo M.  R.; Souza, Diogo Onofre; Salbego, Christianne G.; Ricart, Carlos André O.; Fontes, Wagner; de Sousa, Marcelo Valle

doi:10.1038/s41598-021-82016-7

Title: In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways

Journal Article · Wed Jan 27 23:00:00 EST 2021 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-021-82016-7 · OSTI ID:1816059

^[1]; Souza, Jaques Miranda F. ^[2]; Hoppe, Juliana Bender ^[3]; Firmino, Marina ^[2]; Auer, Manfred ^[4]; Ataii, Nassim N. ^[4]; da Silva, Leonardo Assis ^[5]; Gaelzer, Mariana Maier ^[6]; Klein, Caroline Peres ^[3]; Mól, Alan R. ^[2]; de Lima, Consuelo M. R. ^[2]; Souza, Diogo Onofre ^[3]; Salbego, Christianne G. ^[3]; Ricart, Carlos André O. ^[2]; Fontes, Wagner ^[2];

^[2]

Univ. of Brasília (Brazil). Inst. of Biology. Dept. of Cell Biology. Lab. of Protein Chemistry and Biochemistry; OSTI
Univ. of Brasília (Brazil). Inst. of Biology. Dept. of Cell Biology. Lab. of Protein Chemistry and Biochemistry
Federal Univ. of Rio Grande do Sul, Porto Alegre (Brazil). Dept. of Biochemistry
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
University of Brasília (Brazil). Inst. of Biological Sciences. Dept. of Cell Biology. Lab. of Electron Microscopy
Univ. of Miami, Miami, FL (United States). Miller School of Medicine

Sex differences in the brain of mammals range from neuroarchitecture through cognition to cellular metabolism. The hippocampus, a structure mostly associated with learning and memory, presents high vulnerability to neurodegeneration and aging. Therefore, we explored basal sex-related differences in the proteome of organotypic hippocampal slice culture, a major in vitro model for studying the cellular and molecular mechanisms related to neurodegenerative disorders. Results suggest a greater prevalence of astrocytic metabolism in females and significant neuronal metabolism in males. The preference for glucose use in glycolysis, pentose phosphate pathway and glycogen metabolism in females and high abundance of mitochondrial respiration subunits in males support this idea. An overall upregulation of lipid metabolism was observed in females. Upregulation of proteins responsible for neuronal glutamate and GABA synthesis, along with synaptic associated proteins, were observed in males. In general, the significant spectrum of pathways known to predominate in neurons or astrocytes, together with the well-known neuronal and glial markers observed, revealed sex-specific metabolic differences in the hippocampus. TEM qualitative analysis might indicate a greater presence of mitochondria at CA1 synapses in females. These findings are crucial to a better understanding of how sex chromosomes can influence the physiology of cultured hippocampal slices and allow us to gain insights into distinct responses of males and females on neurological diseases that present a sex-biased incidence.

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Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1816059

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 11; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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