Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif
Abstract
Apolipoprotein L1 (ApoL1) is a circulating innate immunity protein protecting against trypanosome infection. However, two ApoL1 coding variants are associated with a highly increased risk of chronic kidney disease. Here we present X-ray and NMR structures of the N-terminal domain (NTD) of ApoL1 and of its closest relative ApoL2. In both proteins, four of the five NTD helices form a four-helix core structure which is different from the classical four-helix bundle and from the pore-forming domain of colicin A. The reactivity with a conformation-specific antibody and structural models predict that this four-helix motif is also present in the NTDs of ApoL3 and ApoL4, suggesting related functions within the small ApoL family. The long helix 5 of ApoL1 is conformationally flexible and contains the BH3-like region. This BH3-like α-helix resembles true BH3 domains only in sequence and structure but not in function, since it does not bind to the pro-survival members of the Bcl-2 family, suggesting a Bcl-2-independent role in cytotoxicity. These findings should expedite a more comprehensive structural and functional understanding of the ApoL immune protein family.
- Authors:
-
[2];
[2];
[3];
[3];
[3];
[4];
[2];
[1];
[2]
- Genentech Inc., South San Francisco, CA (United States). Dept. of Structural Biology
- Genentech Inc., South San Francisco, CA (United States). Dept. of Early Discovery Biochemistry
- Genentech Inc., South San Francisco, CA (United States). Dept. of Immunology
- Genentech Inc., South San Francisco, CA (United States). Dept. of Antibody Engineering
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1812048
- Grant/Contract Number:
- AC02-06CH11357; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Communications Biology
- Additional Journal Information:
- Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2399-3642
- Publisher:
- Springer Nature
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; X-ray crystallography; lipoproteins
Citation Formats
Ultsch, Mark, Holliday, Michael J., Gerhardy, Stefan, Moran, Paul, Scales, Suzie J., Gupta, Nidhi, Oltrabella, Francesca, Chiu, Cecilia, Fairbrother, Wayne, Eigenbrot, Charles, and Kirchhofer, Daniel. Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif. United States: N. p., 2021.
Web. doi:10.1038/s42003-021-02387-5.
Ultsch, Mark, Holliday, Michael J., Gerhardy, Stefan, Moran, Paul, Scales, Suzie J., Gupta, Nidhi, Oltrabella, Francesca, Chiu, Cecilia, Fairbrother, Wayne, Eigenbrot, Charles, & Kirchhofer, Daniel. Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif. United States. https://doi.org/10.1038/s42003-021-02387-5
Ultsch, Mark, Holliday, Michael J., Gerhardy, Stefan, Moran, Paul, Scales, Suzie J., Gupta, Nidhi, Oltrabella, Francesca, Chiu, Cecilia, Fairbrother, Wayne, Eigenbrot, Charles, and Kirchhofer, Daniel. Tue .
"Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif". United States. https://doi.org/10.1038/s42003-021-02387-5. https://www.osti.gov/servlets/purl/1812048.
@article{osti_1812048,
title = {Structures of the ApoL1 and ApoL2 N-terminal domains reveal a non-classical four-helix bundle motif},
author = {Ultsch, Mark and Holliday, Michael J. and Gerhardy, Stefan and Moran, Paul and Scales, Suzie J. and Gupta, Nidhi and Oltrabella, Francesca and Chiu, Cecilia and Fairbrother, Wayne and Eigenbrot, Charles and Kirchhofer, Daniel},
abstractNote = {Apolipoprotein L1 (ApoL1) is a circulating innate immunity protein protecting against trypanosome infection. However, two ApoL1 coding variants are associated with a highly increased risk of chronic kidney disease. Here we present X-ray and NMR structures of the N-terminal domain (NTD) of ApoL1 and of its closest relative ApoL2. In both proteins, four of the five NTD helices form a four-helix core structure which is different from the classical four-helix bundle and from the pore-forming domain of colicin A. The reactivity with a conformation-specific antibody and structural models predict that this four-helix motif is also present in the NTDs of ApoL3 and ApoL4, suggesting related functions within the small ApoL family. The long helix 5 of ApoL1 is conformationally flexible and contains the BH3-like region. This BH3-like α-helix resembles true BH3 domains only in sequence and structure but not in function, since it does not bind to the pro-survival members of the Bcl-2 family, suggesting a Bcl-2-independent role in cytotoxicity. These findings should expedite a more comprehensive structural and functional understanding of the ApoL immune protein family.},
doi = {10.1038/s42003-021-02387-5},
journal = {Communications Biology},
number = 1,
volume = 4,
place = {United States},
year = {2021},
month = {7}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Apolipoprotein L-I is the trypanosome lytic factor of human serum
journal, March 2003
- Vanhamme, Luc; Paturiaux-Hanocq, Françoise; Poelvoorde, Philippe
- Nature, Vol. 422, Issue 6927
Apolipoprotein L1 (APOL1) risk variant toxicity depends on the haplotype background
journal, December 2019
- Lannon, Herbert; Shah, Shrijal S.; Dias, Leny
- Kidney International, Vol. 96, Issue 6
Redefining the BH3 Death Domain as a ‘Short Linear Motif’
journal, December 2015
- Aouacheria, Abdel; Combet, Christophe; Tompa, Peter
- Trends in Biochemical Sciences, Vol. 40, Issue 12
APOL1: The Balance Imposed by Infection, Selection, and Kidney Disease
journal, August 2018
- Beckerman, Pazit; Susztak, Katalin
- Trends in Molecular Medicine, Vol. 24, Issue 8
Electron diffraction data processing with DIALS
journal, May 2018
- Clabbers, Max T. B.; Gruene, Tim; Parkhurst, James M.
- Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 6
The transcriptome of HCV replicon expressing cell lines in the presence of alpha interferon
journal, May 2005
- Hayashi, Junpei; Stoyanova, Radka; Seeger, Christoph
- Virology, Vol. 335, Issue 2
Overview of the CCP 4 suite and current developments
journal, March 2011
- Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D.
- Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
Intrinsically Disordered Proteins in Bcl-2 Regulated Apoptosis
journal, April 2010
- Rautureau, Gilles J. P.; Day, Catherine L.; Hinds, Mark G.
- International Journal of Molecular Sciences, Vol. 11, Issue 4
ApoL1 Overexpression Drives Variant-Independent Cytotoxicity
journal, November 2017
- O'Toole, John F.; Schilling, William; Kunze, Diana
- Journal of the American Society of Nephrology, Vol. 29, Issue 3
APOL1 Kidney Disease Risk Variants: An Evolving Landscape
journal, May 2015
- Dummer, Patrick D.; Limou, Sophie; Rosenberg, Avi Z.
- Seminars in Nephrology, Vol. 35, Issue 3
Data processing and analysis with the autoPROC toolbox
journal, March 2011
- Vonrhein, Clemens; Flensburg, Claus; Keller, Peter
- Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4
Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice
journal, February 2017
- Beckerman, Pazit; Bi-Karchin, Jing; Park, Ae Seo Deok
- Nature Medicine, Vol. 23, Issue 4
Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions
journal, November 2004
- Krissinel, E.; Henrick, K.
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12
Coupling of lysosomal and mitochondrial membrane permeabilization in trypanolysis by APOL1
journal, August 2015
- Vanwalleghem, Gilles; Fontaine, Frédéric; Lecordier, Laurence
- Nature Communications, Vol. 6, Issue 1
The serum resistance-associated (SRA) gene of Trypanosoma brucei rhodesiense encodes a variant surface glycoprotein-like protein
journal, December 1994
- De Greef, Catherine; Hamers, Raymond
- Molecular and Biochemical Parasitology, Vol. 68, Issue 2
Apolipoprotein L2 contains a BH3-like domain but it does not behave as a BH3-only protein
journal, June 2014
- Galindo-Moreno, J.; Iurlaro, R.; El Mjiyad, N.
- Cell Death & Disease, Vol. 5, Issue 6
The four‐lielix bundle: what determines a fold?
journal, August 1995
- Kamtekar, Satwik; Hecht, Michael H.
- The FASEB Journal, Vol. 9, Issue 11
Torsion angle dynamics for NMR structure calculation with the new program Dyana
journal, October 1997
- Güntert, P.; Mumenthaler, C.; Wüthrich, K.
- Journal of Molecular Biology, Vol. 273, Issue 1
BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins
journal, September 2015
- Heneghan, J. F.; Vandorpe, D. H.; Shmukler, B. E.
- American Journal of Physiology-Cell Physiology, Vol. 309, Issue 5
The function of apolipoproteins L (APOLs): relevance for kidney disease, neurotransmission disorders, cancer and viral infection
journal, June 2020
- Pays, Etienne
- The FEBS Journal, Vol. 288, Issue 2
Apolipoproteins L control cell death triggered by TLR3/TRIF signaling in dendritic cells
journal, June 2016
- Uzureau, Sophie; Coquerelle, Caroline; Vermeiren, Corentin
- European Journal of Immunology, Vol. 46, Issue 8
Crystallizing membrane proteins using lipidic mesophases
journal, April 2009
- Caffrey, Martin; Cherezov, Vadim
- Nature Protocols, Vol. 4, Issue 5
High-Resolution Comparative Modeling with RosettaCM
journal, October 2013
- Song, Yifan; DiMaio, Frank; Wang, Ray Yu-Ruei
- Structure, Vol. 21, Issue 10
Protein domains of APOL1 and its risk variants
journal, August 2015
- Lan, Xiqian; Wen, Hongxiu; Lederman, Rivka
- Experimental and Molecular Pathology, Vol. 99, Issue 1
The function of apolipoproteins L
journal, July 2006
- Vanhollebeke, B.; Pays, E.
- Cellular and Molecular Life Sciences, Vol. 63, Issue 17
A 'molten-globule' membrane-insertion intermediate of the pore-forming domain of colicin A
journal, December 1991
- van der Goot, F. G.; González-Mañas, J. M.; Lakey, J. H.
- Nature, Vol. 354, Issue 6352
Structural characterization of the C-terminal coiled-coil domains of wild-type and kidney disease-associated mutants of apolipoprotein L1
journal, April 2016
- Sharma, Alok K.; Friedman, David J.; Pollak, Martin R.
- The FEBS Journal, Vol. 283, Issue 10
α-Helical coiled coils and bundles: How to design an α-helical protein
journal, January 1990
- Cohen, Carolyn; Parry, David A. D.
- Proteins: Structure, Function, and Genetics, Vol. 7, Issue 1
Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method
journal, June 1997
- Cserzo, M.; Wallin, E.; Simon, I.
- Protein Engineering Design and Selection, Vol. 10, Issue 6
Stereospecific assignment of the methyl 1 H NMR lines of valine and leucine in polypeptides by nonrandom 13 C labelling
journal, May 1989
- Senn, H.; Werner, B.; Messerle, B. A.
- FEBS Letters, Vol. 249, Issue 1
The trypanosome lytic factor of human serum and the molecular basis of sleeping sickness
journal, July 2004
- Vanhamme, Luc; Pays, Etienne
- International Journal for Parasitology, Vol. 34, Issue 8
Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method
journal, June 1997
- Cserzo, M.; Wallin, E.; Simon, I.
- Protein Engineering Design and Selection, Vol. 10, Issue 6
Human trypanolytic factor APOL1 forms pH-gated cation-selective channels in planar lipid bilayers: Relevance to trypanosome lysis
journal, February 2015
- Thomson, Russell; Finkelstein, Alan
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 9
Trypanosome lytic factors: novel mediators of human innate immunity
journal, August 2001
- Raper, J.
- Current Opinion in Microbiology, Vol. 4, Issue 4
Trypanosomatid apoptosis: 'Apoptosis' without the canonical regulators
journal, May 2011
- Smirlis, Despina; Soteriadou, Ketty
- Virulence, Vol. 2, Issue 3
Human High Density Lipoprotein Killing of African Trypanosomes
journal, October 1994
- Hajduk, S. L.; Hager, K. M.; Esko, J. D.
- Annual Review of Microbiology, Vol. 48, Issue 1
A null variant in the apolipoprotein L3 gene is associated with non-diabetic nephropathy
journal, February 2017
- Skorecki, Karl L.; Lee, Jessica H.; Langefeld, Carl D.
- Nephrology Dialysis Transplantation, Vol. 33, Issue 2
Alterations in plasma membrane ion channel structures stimulate NLRP3 inflammasome activation in APOL1 risk milieu
journal, December 2019
- Jha, Alok; Kumar, Vinod; Haque, Shabirul
- The FEBS Journal, Vol. 287, Issue 10
pH-dependent stability and membrane interaction of the pore-forming domain of colicin A.
journal, January 1993
- Muga, A.; Gonzalez-Manas, J. M.; Lakey, J. H.
- Journal of Biological Chemistry, Vol. 268, Issue 3
Apolipoprotein L1, a Novel Bcl-2 Homology Domain 3-only Lipid-binding Protein, Induces Autophagic Cell Death
journal, August 2008
- Wan, Guanghua; Zhaorigetu, Siqin; Liu, Zhihe
- Journal of Biological Chemistry, Vol. 283, Issue 31
Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes
journal, October 2009
- Thomson, R.; Molina-Portela, P.; Mott, H.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 46
PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010
- Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
Version 1.2 of the Crystallography and NMR system
journal, October 2007
- Brunger, Axel T.
- Nature Protocols, Vol. 2, Issue 11
Kidney-disease-associated variants of Apolipoprotein L1 show gain of function in cation channel activity
journal, January 2021
- Bruno, Jonathan; Edwards, John C.
- Journal of Biological Chemistry, Vol. 296
The serum resistance-associated (SRA) gene of Trypanosoma brucei rhodesiense encodes a variant surface glycoprotein-like protein
journal, December 1994
- De Greef, Catherine; Hamers, Raymond
- Molecular and Biochemical Parasitology, Vol. 68, Issue 2
Characterization of primate trypanosome lytic factors
journal, November 2004
- Lugli, Elena B.; Pouliot, Michael; Portela, Maria del Pilar Molina
- Molecular and Biochemical Parasitology, Vol. 138, Issue 1
Apolipoproteins L control cell death triggered by TLR3/TRIF signaling in dendritic cells
journal, June 2016
- Uzureau, Sophie; Coquerelle, Caroline; Vermeiren, Corentin
- European Journal of Immunology, Vol. 46, Issue 8
Dilemmas and challenges in apolipoprotein L1 nephropathy research
journal, January 2019
- Kruzel-Davila, Etty; Skorecki, Karl
- Current Opinion in Nephrology and Hypertension, Vol. 28, Issue 1
Evolution of the primate trypanolytic factor APOL1
journal, May 2014
- Thomson, R.; Genovese, G.; Canon, C.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 20
The Relationship between APOL1 Structure and Function: Clinical Implications
journal, November 2020
- Madhavan, Sethu M.; Buck, Matthias
- Kidney360, Vol. 2, Issue 1
Temporal dissection of Bax‐induced events leading to fission of the single mitochondrion in Trypanosoma brucei
journal, March 2004
- Esseiva, Anne Crausaz; Chanez, Anne‐Laure; Bochud‐Allemann, Natacha
- EMBO reports, Vol. 5, Issue 3
The CCPN data model for NMR spectroscopy: Development of a software pipeline
journal, April 2005
- Vranken, Wim F.; Boucher, Wayne; Stevens, Tim J.
- Proteins: Structure, Function, and Bioinformatics, Vol. 59, Issue 4
BCL-2 family proteins: changing partners in the dance towards death
journal, November 2017
- Kale, Justin; Osterlund, Elizabeth J.; Andrews, David W.
- Cell Death & Differentiation, Vol. 25, Issue 1
TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts
journal, June 2009
- Shen, Yang; Delaglio, Frank; Cornilescu, Gabriel
- Journal of Biomolecular NMR, Vol. 44, Issue 4
Jalview Version 2--a multiple sequence alignment editor and analysis workbench
journal, January 2009
- Waterhouse, A. M.; Procter, J. B.; Martin, D. M. A.
- Bioinformatics, Vol. 25, Issue 9
X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death
journal, May 1996
- Muchmore, Steven W.; Sattler, Michael; Liang, Heng
- Nature, Vol. 381, Issue 6580
A VSG Expression Site–Associated Gene Confers Resistance to Human Serum in Trypanosoma rhodesiense
journal, December 1998
- Van Xong, Hoang; Vanhamme, Luc; Chamekh, Mustapha
- Cell, Vol. 95, Issue 6
Torsion angle dynamics for NMR structure calculation with the new program Dyana
journal, October 1997
- Güntert, P.; Mumenthaler, C.; Wüthrich, K.
- Journal of Molecular Biology, Vol. 273, Issue 1
Redefining the BH3 Death Domain as a ‘Short Linear Motif’
journal, December 2015
- Aouacheria, Abdel; Combet, Christophe; Tompa, Peter
- Trends in Biochemical Sciences, Vol. 40, Issue 12
The BCL-2 Family Reunion
journal, February 2010
- Chipuk, Jerry E.; Moldoveanu, Tudor; Llambi, Fabien
- Molecular Cell, Vol. 37, Issue 3
Apolipoprotein L, a New Human High Density Lipoprotein Apolipoprotein Expressed by the Pancreas
journal, October 1997
- Duchateau, Philippe N.; Pullinger, Clive R.; Orellana, Roberto E.
- Journal of Biological Chemistry, Vol. 272, Issue 41
APOL1 Kidney Disease Risk Variants: An Evolving Landscape
journal, May 2015
- Dummer, Patrick D.; Limou, Sophie; Rosenberg, Avi Z.
- Seminars in Nephrology, Vol. 35, Issue 3
Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity
dataset, January 2020
- Giovinazzo, Joseph; Giovinazzo, Joseph A.; Thomson, Russell P.
- Dryad
Identification of the trypanocidal factor in normal human serum: high density lipoprotein.
journal, July 1978
- Rifkin, M. R.
- Proceedings of the National Academy of Sciences, Vol. 75, Issue 7
Characterization of a Novel Trypanosome Lytic Factor from Human Serum
journal, January 1999
- Raper, Jayne; Fung, Ramie; Ghiso, Jorge
- Infection and Immunity, Vol. 67, Issue 4
APOL1 renal risk variants have contrasting resistance and susceptibility associations with African trypanosomiasis
journal, May 2017
- Cooper, Anneli; Ilboudo, Hamidou; Alibu, V. Pius
- eLife, Vol. 6
The transcriptome of HCV replicon expressing cell lines in the presence of alpha interferon
journal, May 2005
- Hayashi, Junpei; Stoyanova, Radka; Seeger, Christoph
- Virology, Vol. 335, Issue 2
High-Resolution Comparative Modeling with RosettaCM
journal, October 2013
- Song, Yifan; DiMaio, Frank; Wang, Ray Yu-Ruei
- Structure, Vol. 21, Issue 10
Innate immunity pathways regulate the nephropathy gene Apolipoprotein L1
journal, February 2015
- Nichols, Brendan; Jog, Prachi; Lee, Jessica H.
- Kidney International, Vol. 87, Issue 2
A Survey for a Trypanocidal Factor in Primate Sera
journal, September 1990
- Seed, John R.; Sechelski, Ohn B.; Loomis, M. R.
- The Journal of Protozoology, Vol. 37, Issue 5
A 'molten-globule' membrane-insertion intermediate of the pore-forming domain of colicin A
journal, December 1991
- van der Goot, F. G.; González-Mañas, J. M.; Lakey, J. H.
- Nature, Vol. 354, Issue 6352
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
journal, September 1998
- Brünger, A. T.; Adams, P. D.; Clore, G. M.
- Acta Crystallographica Section D Biological Crystallography, Vol. 54, Issue 5
Intrinsically Disordered Proteins in Bcl-2 Regulated Apoptosis
journal, April 2010
- Rautureau, Gilles J. P.; Day, Catherine L.; Hinds, Mark G.
- International Journal of Molecular Sciences, Vol. 11, Issue 4
Control of apoptosis by the BCL-2 protein family: implications for physiology and therapy
journal, December 2013
- Czabotar, Peter E.; Lessene, Guillaume; Strasser, Andreas
- Nature Reviews Molecular Cell Biology, Vol. 15, Issue 1
APOL1: The Balance Imposed by Infection, Selection, and Kidney Disease
journal, August 2018
- Beckerman, Pazit; Susztak, Katalin
- Trends in Molecular Medicine, Vol. 24, Issue 8
Apolipoprotein L1 (APOL1) risk variant toxicity depends on the haplotype background
journal, December 2019
- Lannon, Herbert; Shah, Shrijal S.; Dias, Leny
- Kidney International, Vol. 96, Issue 6
Distribution of apolipoprotein L-I and trypanosome lytic activity among primate sera
journal, March 2004
- Poelvoorde, Philippe; Vanhamme, Luc; Abbeele, Jan Van Den
- Molecular and Biochemical Parasitology, Vol. 134, Issue 1
Coupling of lysosomal and mitochondrial membrane permeabilization in trypanolysis by APOL1
journal, August 2015
- Vanwalleghem, Gilles; Fontaine, Frédéric; Lecordier, Laurence
- Nature Communications, Vol. 6, Issue 1
Refined structure of the pore-forming domain of colicin A at 2.4 Å resolution
journal, April 1992
- Parker, Michael W.; Postma, Johan P. M.; Pattus, Franc
- Journal of Molecular Biology, Vol. 224, Issue 3
The apolipoprotein L family of programmed cell death and immunity genes rapidly evolved in primates at discrete sites of host-pathogen interactions
journal, March 2009
- Smith, E. E.; Malik, H. S.
- Genome Research, Vol. 19, Issue 5
The molecular arms race between African trypanosomes and humans
journal, June 2014
- Pays, Etienne; Vanhollebeke, Benoit; Uzureau, Pierrick
- Nature Reviews Microbiology, Vol. 12, Issue 8
JPred4: a protein secondary structure prediction server
journal, April 2015
- Drozdetskiy, Alexey; Cole, Christian; Procter, James
- Nucleic Acids Research, Vol. 43, Issue W1
Missense mutations in the APOL1 gene are highly associated with end stage kidney disease risk previously attributed to the MYH9 gene
journal, July 2010
- Tzur, Shay; Rosset, Saharon; Shemer, Revital
- Human Genetics, Vol. 128, Issue 3
Biogenesis and cytotoxicity of APOL1 renal risk variant proteins in hepatocytes and hepatoma cells
journal, August 2015
- Cheng, Dongmei; Weckerle, Allison; Yu, Yi
- Journal of Lipid Research, Vol. 56, Issue 8
Jalview Version 2--a multiple sequence alignment editor and analysis workbench
journal, January 2009
- Waterhouse, A. M.; Procter, J. B.; Martin, D. M. A.
- Bioinformatics, Vol. 25, Issue 9
The structure of serum resistance-associated protein and its implications for human African trypanosomiasis
journal, January 2018
- Zoll, Sebastian; Lane-Serff, Harriet; Mehmood, Shahid
- Nature Microbiology, Vol. 3, Issue 3
APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8
journal, July 2017
- Madhavan, Sethu M.; O’Toole, John F.; Konieczkowski, Martha
- JCI Insight, Vol. 2, Issue 14
APOL1 kidney disease risk variants cause cytotoxicity by depleting cellular potassium and inducing stress-activated protein kinases
journal, December 2015
- Olabisi, Opeyemi A.; Zhang, Jia-Yue; VerPlank, Lynn
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 4
Characterization of a Novel Trypanosome Lytic Factor from Human Serum
journal, April 1999
- Raper, Jayne; Fung, Ramie; Ghiso, Jorge
- Infection and Immunity, Vol. 67, Issue 4
Apolipoprotein L gene family: tissue-specific expression, splicing, promoter regions; discovery of a new gene
journal, April 2001
- Duchateau, Philippe N.; Pullinger, Clive R.; Cho, Min H.
- Journal of Lipid Research, Vol. 42, Issue 4
The Human Apolipoprotein L Gene Cluster: Identification, Classification, and Sites of Distribution
journal, May 2001
- Page, Nigel M.; Butlin, David J.; Lomthaisong, Khomsorn
- Genomics, Vol. 74, Issue 1
Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity
journal, May 2020
- Giovinazzo, Joseph A.; Thomson, Russell P.; Khalizova, Nailya
- eLife, Vol. 9
Stereospecific assignment of the methyl 1 H NMR lines of valine and leucine in polypeptides by nonrandom 13 C labelling
journal, May 1989
- Senn, H.; Werner, B.; Messerle, B. A.
- FEBS Letters, Vol. 249, Issue 1
APOL1 and kidney cell function
journal, August 2019
- Kumar, Vinod; Singhal, Pravin C.
- American Journal of Physiology-Renal Physiology, Vol. 317, Issue 2
The trypanosome lytic factor of human serum and the molecular basis of sleeping sickness
journal, July 2004
- Vanhamme, Luc; Pays, Etienne
- International Journal for Parasitology, Vol. 34, Issue 8
Cation channel conductance and pH gating of the innate immunity factor APOL1 are governed by pore-lining residues within the C-terminal domain
journal, September 2020
- Schaub, Charles; Verdi, Joseph; Lee, Penny
- Journal of Biological Chemistry, Vol. 295, Issue 38
Trypanosomatid apoptosis: 'Apoptosis' without the canonical regulators
journal, May 2011
- Smirlis, Despina; Soteriadou, Ketty
- Virulence, Vol. 2, Issue 3
Protein NMR Structure Determination with Automated NOE Assignment Using the New Software CANDID and the Torsion Angle Dynamics Algorithm DYANA
journal, May 2002
- Herrmann, Torsten; Güntert, Peter; Wüthrich, Kurt
- Journal of Molecular Biology, Vol. 319, Issue 1
APOL1 toxin, innate immunity, and kidney injury
journal, July 2015
- Limou, Sophie; Dummer, Patrick D.; Nelson, George W.
- Kidney International, Vol. 88, Issue 1
Crystallizing membrane proteins using lipidic mesophases
journal, April 2009
- Caffrey, Martin; Cherezov, Vadim
- Nature Protocols, Vol. 4, Issue 5
Apolipoprotein L1 confers pH-switchable ion permeability to phospholipid vesicles
journal, November 2017
- Bruno, Jonathan; Pozzi, Nicola; Oliva, Jonathan
- Journal of Biological Chemistry, Vol. 292, Issue 44
Mechanisms of Injury in APOL1-associated Kidney Disease
journal, January 2019
- Ma, Lijun; Divers, Jasmin; Freedman, Barry I.
- Transplantation, Vol. 103, Issue 3
Features and development of Coot
journal, March 2010
- Emsley, P.; Lohkamp, B.; Scott, W. G.
- Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts
journal, June 2009
- Shen, Yang; Delaglio, Frank; Cornilescu, Gabriel
- Journal of Biomolecular NMR, Vol. 44, Issue 4
X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death
journal, May 1996
- Muchmore, Steven W.; Sattler, Michael; Liang, Heng
- Nature, Vol. 381, Issue 6580
Benchmarking fold detection by DaliLite v.5
journal, July 2019
- Holm, Liisa
- Bioinformatics, Vol. 35, Issue 24
A combined approach to improving large-scale production of tobacco etch virus protease
journal, September 2007
- Blommel, Paul G.; Fox, Brian G.
- Protein Expression and Purification, Vol. 55, Issue 1, p. 53-68
ApoL1 induces kidney inflammation through RIG-I/NF-κB activation
journal, June 2020
- Fang, Ji; Yao, Xingmei; Hou, Mingqiang
- Biochemical and Biophysical Research Communications, Vol. 527, Issue 2
Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes
journal, August 2020
- Gupta, Nidhi; Wang, Xinhua; Wen, Xiaohui
- Journal of the American Society of Nephrology, Vol. 31, Issue 9
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega
journal, January 2011
- Sievers, Fabian; Wilm, Andreas; Dineen, David
- Molecular Systems Biology, Vol. 7, Issue 1
Humanization of an anti-p185HER2 antibody for human cancer therapy.
journal, May 1992
- Carter, P.; Presta, L.; Gorman, C. M.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 10, p. 4285-4289
The function of apolipoproteins L
journal, July 2006
- Vanhollebeke, B.; Pays, E.
- Cellular and Molecular Life Sciences, Vol. 63, Issue 17
APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin
journal, March 2020
- Uzureau, Sophie; Lecordier, Laurence; Uzureau, Pierrick
- Cell Reports, Vol. 30, Issue 11
The Cell Biology of APOL1
journal, November 2017
- O’Toole, John F.; Bruggeman, Leslie A.; Madhavan, Sethu
- Seminars in Nephrology, Vol. 37, Issue 6
Association of Trypanolytic ApoL1 Variants with Kidney Disease in African Americans
journal, July 2010
- Genovese, G.; Friedman, D. J.; Ross, M. D.
- Science, Vol. 329, Issue 5993
Gene of the month: APOL1
journal, May 2020
- Raghubeer, Shanel; Pillay, Tahir S.; Matsha, Tandi Edith
- Journal of Clinical Pathology, Vol. 73, Issue 8
Trypanosome lytic factor, a subclass of high-density lipoprotein, forms cation-selective pores in membranes
journal, December 2005
- del Pilar Molina-Portela, Maria; Lugli, Elena B.; Recio-Pinto, Esperanza
- Molecular and Biochemical Parasitology, Vol. 144, Issue 2
Identification of the trypanocidal factor in normal human serum: high density lipoprotein.
journal, July 1978
- Rifkin, M. R.
- Proceedings of the National Academy of Sciences, Vol. 75, Issue 7
BH3 domain-independent apolipoprotein L1 toxicity rescued by BCL2 prosurvival proteins
journal, September 2015
- Heneghan, J. F.; Vandorpe, D. H.; Shmukler, B. E.
- American Journal of Physiology-Cell Physiology, Vol. 309, Issue 5
Phaser crystallographic software
journal, July 2007
- McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
- Journal of Applied Crystallography, Vol. 40, Issue 4
Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes
journal, August 2020
- Scales, Suzie J.; Gupta, Nidhi; De Mazière, Ann M.
- Journal of the American Society of Nephrology, Vol. 31, Issue 9
Apolipoprotein L-I Promotes Trypanosome Lysis by Forming Pores in Lysosomal Membranes
journal, July 2005
- Perez-Morga, D.
- Science, Vol. 309, Issue 5733
Apolipoprotein L6, a Novel Proapoptotic Bcl-2 Homology 3–Only Protein, Induces Mitochondria-Mediated Apoptosis in Cancer Cells
journal, January 2005
- Liu, Zhihe; Lu, Huimei; Jiang, Zeyu
- Molecular Cancer Research, Vol. 3, Issue 1