Broad betacoronavirus neutralization by a stem helix–specific human antibody

Pinto, Dora; Sauer, Maximilian M.; Czudnochowski, Nadine; Low, Jun Siong; Tortorici, M. Alejandra; Housley, Michael P.; Noack, Julia; Walls, Alexandra C.; Bowen, John E.; Guarino, Barbara; Rosen, Laura E.; di Iulio, Julia; Jerak, Josipa; Kaiser, Hannah; Islam, Saiful; Jaconi, Stefano; Sprugasci, Nicole; Culap, Katja; Abdelnabi, Rana; Foo, Caroline; Coelmont, Lotte; Bartha, Istvan; Bianchi, Siro; Silacci-Fregni, Chiara; Bassi, Jessica; Marzi, Roberta; Vetti, Eneida; Cassotta, Antonino; Ceschi, Alessandro; Ferrari, Paolo; Cippà, Pietro E.; Giannini, Olivier; Ceruti, Samuele; Garzoni, Christian; Riva, Agostino; Benigni, Fabio; Cameroni, Elisabetta; Piccoli, Luca; Pizzuto, Matteo S.; Smithey, Megan; Hong, David; Telenti, Amalio; Lempp, Florian A.; Neyts, Johan; Havenar-Daughton, Colin; Lanzavecchia, Antonio; Sallusto, Federica; Snell, Gyorgy; Virgin, Herbert W.; Beltramello, Martina; Corti, Davide; Veesler, David

doi:10.1126/science.abj3321

Title: Broad betacoronavirus neutralization by a stem helix–specific human antibody

Journal Article · Fri Sep 03 00:00:00 UTC 2021 · Science

DOI: https://doi.org/10.1126/science.abj3321 · OSTI ID:1811721

Pinto, Dora ^[1];

^[2];

^[3];

^[4];

^[2]; Housley, Michael P. ^[3];

^[3];

^[2];

^[1];

^[3];

^[4];

^[3]; Islam, Saiful ^[3]; Jaconi, Stefano ^[1];

^[1];

^[5];

^[5] more »

Humabs Biomed SA, a subsidiary of Vir Biotechnology, 6500 Bellinzona, Switzerland.
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
Vir Biotechnology, San Francisco, CA 94158, USA.
Institute for Research in Biomedicine, Università della Svizzera italiana, 6500 Bellinzona, Switzerland.
Rega Institute for Medical Research, Laboratory of Virology and Chemotherapy, KU Leuven, 3000 Leuven, Belgium.
Clinical Trial Unit, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland., Division of Clinical Pharmacology and Toxicology, Institute of Pharmacological Sciences of Southern Switzerland, Ente Ospedaliero Cantonale, 6900 Lugano, Switzerland., Department of Clinical Pharmacology and Toxicology, University Hospital Zurich, 8091 Zurich, Switzerland., Faculty of Biomedical Sciences, Università della Svizzera italiana, 6900 Lugano, Switzerland.
Faculty of Biomedical Sciences, Università della Svizzera italiana, 6900 Lugano, Switzerland., Department of Medicine, Ente Ospedaliero Cantonale, 6500 Bellinzona, Switzerland., Clinical School, University of New South Wales, Sydney, NSW, 2052, Australia.
Department of Medicine, Ente Ospedaliero Cantonale, 6500 Bellinzona, Switzerland., Faculty of Medicine, University of Zurich, 8057 Zurich, Switzerland.
Faculty of Biomedical Sciences, Università della Svizzera italiana, 6900 Lugano, Switzerland., Department of Medicine, Ente Ospedaliero Cantonale, 6500 Bellinzona, Switzerland.
Intensive Care Unit, Clinica Luganese Moncucco, 6900 Lugano, Switzerland.
Clinic of Internal Medicine and Infectious Diseases, Clinica Luganese Moncucco, 6900 Lugano, Switzerland.
III Division of Infectious Diseases, ASST Fatebenefratelli Sacco, Luigi Sacco Hospital, 20157 Milan, Italy.
Institute for Research in Biomedicine, Università della Svizzera italiana, 6500 Bellinzona, Switzerland., Institute of Microbiology, ETH Zurich, 8093 Zurich, Switzerland.
Vir Biotechnology, San Francisco, CA 94158, USA., UT Southwestern Medical Center, Dallas, TX 75390, USA., Washington University School of Medicine, St. Louis, MO 63110, USA.

Targeting a range of betacoranaviruses In the past 20 years, three highly pathogenic β-coronaviruses have crossed from animals to humans, including the most recent: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A spike protein that decorates these viruses has an S1 domain that binds host cell receptors and an S2 domain that fuses the viral and cell membranes to allow cell entry. The S1 domain is the target of many neutralizing antibodies but is more genetically variable than S2, and antibodies can exert selective pressure, leading to resistant variants. Pinto et al . identified five monoclonal antibodies that interact with a helix in the S2 domain. The most broadly neutralizing antibody inhibited all β-coronavirus subgenera and reduced viral burden in hamsters infected with SARS-CoV-2. —VV

View Journal Article

Cite

Export

Save

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL); University of Washington, Seattle, WA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1811721

Journal Information:: Science, Journal Name: Science Journal Issue: 6559 Vol. 373; ISSN 0036-8075

Publisher:: American Association for the Advancement of Science (AAAS)Copyright Statement

Country of Publication:: United States

Language:: English

References (79)

Clonal dissection of the human memory B-cell repertoire following infection and vaccination Pinna, Debora; Corti, Davide; Jarrossay, David European Journal of Immunology, Vol. 39, Issue 5 https://doi.org/10.1002/eji.200839129	journal	April 2009
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Structural insights into coronavirus entry Tortorici, M. Alejandra; Veesler, David Advances in Virus Research https://doi.org/10.1016/bs.aivir.2019.08.002	book	January 2019
Structure and Function Analysis of an Antibody Recognizing All Influenza A Subtypes Kallewaard, Nicole L.; Corti, Davide; Collins, Patrick J. Cell, Vol. 166, Issue 3 https://doi.org/10.1016/j.cell.2016.05.073	journal	July 2016
Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion Walls, Alexandra C.; Xiong, Xiaoli; Park, Young-Jun Cell, Vol. 176, Issue 5 https://doi.org/10.1016/j.cell.2018.12.028	journal	February 2019
Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus Marcandalli, Jessica; Fiala, Brooke; Ols, Sebastian Cell, Vol. 176, Issue 6 https://doi.org/10.1016/j.cell.2019.01.046	journal	March 2019
SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor Hoffmann, Markus; Kleine-Weber, Hannah; Schroeder, Simon Cell, Vol. 181, Issue 2 https://doi.org/10.1016/j.cell.2020.02.052	journal	April 2020
Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein Walls, Alexandra C.; Park, Young-Jun; Tortorici, M. Alejandra Cell, Vol. 181, Issue 2 https://doi.org/10.1016/j.cell.2020.02.058	journal	April 2020
Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology Piccoli, Luca; Park, Young-Jun; Tortorici, M. Alejandra Cell, Vol. 183, Issue 4 https://doi.org/10.1016/j.cell.2020.09.037	journal	November 2020
Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2 Walls, Alexandra C.; Fiala, Brooke; Schäfer, Alexandra Cell, Vol. 183, Issue 5 https://doi.org/10.1016/j.cell.2020.10.043	journal	November 2020
Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity Thomson, Emma C.; Rosen, Laura E.; Shepherd, James G. Cell, Vol. 184, Issue 5 https://doi.org/10.1016/j.cell.2021.01.037	journal	March 2021
Human neutralizing antibodies against SARS-CoV-2 require intact Fc effector functions for optimal therapeutic protection Winkler, Emma S.; Gilchuk, Pavlo; Yu, Jinsheng Cell, Vol. 184, Issue 7 https://doi.org/10.1016/j.cell.2021.02.026	journal	April 2021
N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2 McCallum, Matthew; De Marco, Anna; Lempp, Florian A. Cell, Vol. 184, Issue 9 https://doi.org/10.1016/j.cell.2021.03.028	journal	April 2021
Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Competent VSV-SARS-CoV-2 and a Clinical Isolate of SARS-CoV-2 Case, James Brett; Rothlauf, Paul W.; Chen, Rita E. Cell Host & Microbe, Vol. 28, Issue 3 https://doi.org/10.1016/j.chom.2020.06.021	journal	September 2020
Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization Liu, Zhuoming; VanBlargan, Laura A.; Bloyet, Louis-Marie Cell Host & Microbe, Vol. 29, Issue 3 https://doi.org/10.1016/j.chom.2021.01.014	journal	March 2021
Comparing infectivity and virulence of emerging SARS-CoV-2 variants in Syrian hamsters Abdelnabi, Rana; Boudewijns, Robbert; Foo, Caroline S. EBioMedicine, Vol. 68 https://doi.org/10.1016/j.ebiom.2021.103403	journal	June 2021
Automated molecular microscopy: The new Leginon system Suloway, Christian; Pulokas, James; Fellmann, Denis Journal of Structural Biology, Vol. 151, Issue 1 https://doi.org/10.1016/j.jsb.2005.03.010	journal	July 2005
A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells Hoffmann, Markus; Kleine-Weber, Hannah; Pöhlmann, Stefan Molecular Cell, Vol. 78, Issue 4 https://doi.org/10.1016/j.molcel.2020.04.022	journal	May 2020
High-resolution noise substitution to measure overfitting and validate resolution in 3D structure determination by single particle electron cryomicroscopy Chen, Shaoxia; McMullan, Greg; Faruqi, Abdul R. Ultramicroscopy, Vol. 135 https://doi.org/10.1016/j.ultramic.2013.06.004	journal	December 2013
Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding Gui, Miao; Song, Wenfei; Zhou, Haixia Cell Research, Vol. 27, Issue 1 https://doi.org/10.1038/cr.2016.152	journal	December 2016
Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies Kanekiyo, Masaru; Wei, Chih-Jen; Yassine, Hadi M. Nature, Vol. 499, Issue 7456 https://doi.org/10.1038/nature12202	journal	May 2013
Proof of principle for epitope-focused vaccine design Correia, Bruno E.; Bates, John T.; Loomis, Rebecca J. Nature, Vol. 507, Issue 7491 https://doi.org/10.1038/nature12966	journal	February 2014
Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer Walls, Alexandra C.; Tortorici, M. Alejandra; Bosch, Berend-Jan Nature, Vol. 531, Issue 7592 https://doi.org/10.1038/nature16988	journal	February 2016
Nextflow enables reproducible computational workflows Di Tommaso, Paolo; Chatzou, Maria; Floden, Evan W. Nature Biotechnology, Vol. 35, Issue 4 https://doi.org/10.1038/nbt.3820	journal	April 2017
Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains Yuan, Yuan; Cao, Duanfang; Zhang, Yanfang Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15092	journal	April 2017
Broadly neutralizing hemagglutinin stalk–specific antibodies require FcγR interactions for protection against influenza virus in vivo DiLillo, David J.; Tan, Gene S.; Palese, Peter Nature Medicine, Vol. 20, Issue 2 https://doi.org/10.1038/nm.3443	journal	January 2014
A public antibody lineage that potently inhibits malaria infection through dual binding to the circumsporozoite protein Tan, Joshua; Sack, Brandon K.; Oyen, David Nature Medicine, Vol. 24, Issue 4 https://doi.org/10.1038/nm.4513	journal	March 2018
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination Punjani, Ali; Rubinstein, John L.; Fleet, David J. Nature Methods, Vol. 14, Issue 3 https://doi.org/10.1038/nmeth.4169	journal	February 2017
Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients Poh, Chek Meng; Carissimo, Guillaume; Wang, Bei Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-16638-2	journal	June 2020
Cryo-EM analysis of the post-fusion structure of the SARS-CoV spike glycoprotein Fan, Xiaoyi; Cao, Duanfang; Kong, Lingfei Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-17371-6	journal	July 2020
STAT2 signaling restricts viral dissemination but drives severe pneumonia in SARS-CoV-2 infected hamsters Boudewijns, Robbert; Thibaut, Hendrik Jan; Kaptein, Suzanne J. F. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-19684-y	journal	November 2020
A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies Wang, Chunyan; van Haperen, Rien; Gutiérrez-Álvarez, Javier Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21968-w	journal	March 2021
Cross-reactive serum and memory B-cell responses to spike protein in SARS-CoV-2 and endemic coronavirus infection Song, Ge; He, Wan-ting; Callaghan, Sean Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-23074-3	journal	May 2021
Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody Pinto, Dora; Park, Young-Jun; Beltramello, Martina Nature, Vol. 583, Issue 7815 https://doi.org/10.1038/s41586-020-2349-y	journal	May 2020
Chloroquine does not inhibit infection of human lung cells with SARS-CoV-2 Hoffmann, Markus; Mösbauer, Kirstin; Hofmann-Winkler, Heike Nature, Vol. 585, Issue 7826 https://doi.org/10.1038/s41586-020-2575-3	journal	July 2020
Fc-optimized antibodies elicit CD8 immunity to viral respiratory infection Bournazos, Stylianos; Corti, Davide; Virgin, Herbert W. Nature, Vol. 588, Issue 7838 https://doi.org/10.1038/s41586-020-2838-z	journal	October 2020
mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants Wang, Zijun; Schmidt, Fabian; Weisblum, Yiska Nature, Vol. 592, Issue 7855 https://doi.org/10.1038/s41586-021-03324-6	journal	February 2021
Quadrivalent influenza nanoparticle vaccines induce broad protection Boyoglu-Barnum, Seyhan; Ellis, Daniel; Gillespie, Rebecca A. Nature, Vol. 592, Issue 7855 https://doi.org/10.1038/s41586-021-03365-x	journal	March 2021
Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses Kanekiyo, Masaru; Joyce, M. Gordon; Gillespie, Rebecca A. Nature Immunology, Vol. 20, Issue 3 https://doi.org/10.1038/s41590-018-0305-x	journal	February 2019
Bioconda: sustainable and comprehensive software distribution for the life sciences Grüning, Björn; Dale, Ryan; Sjödin, Andreas Nature Methods, Vol. 15, Issue 7 https://doi.org/10.1038/s41592-018-0046-7	journal	July 2018
Real-time cryo-electron microscopy data preprocessing with Warp Tegunov, Dimitry; Cramer, Patrick Nature Methods, Vol. 16, Issue 11 https://doi.org/10.1038/s41592-019-0580-y	journal	October 2019
Structural basis for human coronavirus attachment to sialic acid receptors Tortorici, M. Alejandra; Walls, Alexandra C.; Lang, Yifei Nature Structural & Molecular Biology, Vol. 26, Issue 6 https://doi.org/10.1038/s41594-019-0233-y	journal	June 2019
Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors Park, Young-Jun; Walls, Alexandra C.; Wang, Zhaoqian Nature Structural & Molecular Biology, Vol. 26, Issue 12 https://doi.org/10.1038/s41594-019-0334-7	journal	December 2019
Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis Kirchdoerfer, Robert N.; Wang, Nianshuang; Pallesen, Jesper Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-34171-7	journal	October 2018
Persistence and Evolution of SARS-CoV-2 in an Immunocompromised Host Choi, Bina; Choudhary, Manish C.; Regan, James New England Journal of Medicine, Vol. 383, Issue 23 https://doi.org/10.1056/NEJMc2031364	journal	December 2020
Tectonic conformational changes of a coronavirus spike glycoprotein promote membrane fusion Walls, Alexandra C.; Tortorici, M. Alejandra; Snijder, Joost Proceedings of the National Academy of Sciences, Vol. 114, Issue 42 https://doi.org/10.1073/pnas.1708727114	journal	October 2017
Antibody potency, effector function, and combinations in protection and therapy for SARS-CoV-2 infection in vivo Schäfer, Alexandra; Muecksch, Frauke; Lorenzi, Julio C. C. Journal of Experimental Medicine, Vol. 218, Issue 3 https://doi.org/10.1084/jem.20201993	journal	November 2020
Trimmomatic: a flexible trimmer for Illumina sequence data Bolger, Anthony M.; Lohse, Marc; Usadel, Bjoern Bioinformatics, Vol. 30, Issue 15 https://doi.org/10.1093/bioinformatics/btu170	journal	April 2014
MultiQC: summarize analysis results for multiple tools and samples in a single report Ewels, Philip; Magnusson, Måns; Lundin, Sverker Bioinformatics, Vol. 32, Issue 19 https://doi.org/10.1093/bioinformatics/btw354	journal	June 2016
Mosdepth: quick coverage calculation for genomes and exomes Pedersen, Brent S.; Quinlan, Aaron R. Bioinformatics, Vol. 34, Issue 5 https://doi.org/10.1093/bioinformatics/btx699	journal	October 2017
Twelve years of SAMtools and BCFtools Danecek, Petr; Bonfield, James K.; Liddle, Jennifer GigaScience, Vol. 10, Issue 2 https://doi.org/10.1093/gigascience/giab008	journal	January 2021
LoFreq: a sequence-quality aware, ultra-sensitive variant caller for uncovering cell-population heterogeneity from high-throughput sequencing datasets Wilm, Andreas; Aw, Pauline Poh Kim; Bertrand, Denis Nucleic Acids Research, Vol. 40, Issue 22 https://doi.org/10.1093/nar/gks918	journal	October 2012
A Simple Method of Estimating Fifty per cent Endpoints12 Reed, L. J.; Muench, H. American Journal of Epidemiology, Vol. 27, Issue 3 https://doi.org/10.1093/oxfordjournals.aje.a118408	journal	May 1938
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
REFMAC 5 for the refinement of macromolecular crystal structures Murshudov, Garib N.; Skubák, Pavol; Lebedev, Andrey A. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444911001314	journal	March 2011
A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis Zivanov, Jasenko; Nakane, Takanori; Scheres, Sjors H. W. IUCrJ, Vol. 6, Issue 1 https://doi.org/10.1107/S205225251801463X	journal	January 2019
ISOLDE : a physically realistic environment for model building into low-resolution electron-density maps Croll, Tristan Ian Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 6 https://doi.org/10.1107/S2059798318002425	journal	April 2018
Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix Liebschner, Dorothee; Afonine, Pavel V.; Baker, Matthew L. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 10 https://doi.org/10.1107/S2059798319011471	journal	October 2019
A Neutralizing Antibody Selected from Plasma Cells That Binds to Group 1 and Group 2 Influenza A Hemagglutinins Corti, D.; Voss, J.; Gamblin, S. J. Science, Vol. 333, Issue 6044 https://doi.org/10.1126/science.1205669	journal	July 2011
Computation-Guided Backbone Grafting of a Discontinuous Motif onto a Protein Scaffold Azoitei, M. L.; Correia, B. E.; Ban, Y. -E. A. Science, Vol. 334, Issue 6054 https://doi.org/10.1126/science.1209368	journal	October 2011
De novo protein design enables the precise induction of RSV-neutralizing antibodies Sesterhenn, Fabian; Yang, Che; Bonet, Jaume Science, Vol. 368, Issue 6492 https://doi.org/10.1126/science.aay5051	journal	May 2020
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation Wrapp, Daniel; Wang, Nianshuang; Corbett, Kizzmekia S. Science, Vol. 367, Issue 6483 https://doi.org/10.1126/science.abb2507	journal	February 2020
Site-specific glycan analysis of the SARS-CoV-2 spike Watanabe, Yasunori; Allen, Joel D.; Wrapp, Daniel Science, Vol. 369, Issue 6501 https://doi.org/10.1126/science.abb9983	journal	May 2020
Distinct conformational states of SARS-CoV-2 spike protein Cai, Yongfei; Zhang, Jun; Xiao, Tianshu Science https://doi.org/10.1126/science.abd4251	journal	July 2020
Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms Tortorici, M. Alejandra; Beltramello, Martina; Lempp, Florian A. Science, Vol. 370, Issue 6519 https://doi.org/10.1126/science.abe3354	journal	September 2020
Recurrent deletions in the SARS-CoV-2 spike glycoprotein drive antibody escape McCarthy, Kevin R.; Rennick, Linda J.; Nambulli, Sham Science, Vol. 371, Issue 6534 https://doi.org/10.1126/science.abf6950	journal	February 2021
SARS-CoV-2 immune evasion by the B.1.427/B.1.429 variant of concern McCallum, Matthew; Bassi, Jessica; De Marco, Anna Science https://doi.org/10.1126/science.abi7994	journal	July 2021
Multiple roles for HIV broadly neutralizing antibodies Haynes, Barton F.; Burton, Dennis R.; Mascola, John R. Science Translational Medicine, Vol. 11, Issue 516 https://doi.org/10.1126/scitranslmed.aaz2686	journal	October 2019
Two Mutations Were Critical for Bat-to-Human Transmission of Middle East Respiratory Syndrome Coronavirus Yang, Yang; Liu, Chang; Du, Lanying Journal of Virology, Vol. 89, Issue 17 https://doi.org/10.1128/JVI.01279-15	journal	June 2015
Acquisition of Complement Resistance through Incorporation of CD55/Decay-Accelerating Factor into Viral Particles Bearing Baculovirus GP64 Kaname, Yuuki; Tani, Hideki; Kataoka, Chikako Journal of Virology, Vol. 84, Issue 7 https://doi.org/10.1128/JVI.02519-09	journal	April 2010
Identification of an Antigenic Determinant on the S2 Domain of the Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Capable of Inducing Neutralizing Antibodies Zhang, H.; Wang, G.; Li, J. Journal of Virology, Vol. 78, Issue 13 https://doi.org/10.1128/JVI.78.13.6938-6945.2004	journal	June 2004
Identification of an immunodominant linear neutralization domain on the S2 portion of the murine coronavirus spike glycoprotein and evidence that it forms part of complex tridimensional structure. Daniel, C.; Anderson, R.; Buchmeier, M. J. Journal of Virology, Vol. 67, Issue 3 https://doi.org/10.1128/jvi.67.3.1185-1194.1993	journal	January 1993
The Role and Function of Fcγ Receptors on Myeloid Cells Bournazos, Stylianos; Wang, Taia T.; Ravetch, Jeffrey V. Microbiology Spectrum, Vol. 4, Issue 6 https://doi.org/10.1128/microbiolspec.MCHD-0045-2016	journal	December 2016
Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccine Corti, Davide; Suguitan, Amorsolo L.; Pinna, Debora Journal of Clinical Investigation, Vol. 120, Issue 5 https://doi.org/10.1172/JCI41902	journal	May 2010
AncesTree: An interactive immunoglobulin lineage tree visualizer Foglierini, Mathilde; Pappas, Leontios; Lanzavecchia, Antonio PLOS Computational Biology, Vol. 16, Issue 7 https://doi.org/10.1371/journal.pcbi.1007731	journal	July 2020
Human Monoclonal Antibodies against Highly Conserved HR1 and HR2 Domains of the SARS-CoV Spike Protein Are More Broadly Neutralizing Elshabrawy, Hatem A.; Coughlin, Melissa M.; Baker, Susan C. PLoS ONE, Vol. 7, Issue 11 https://doi.org/10.1371/journal.pone.0050366	journal	November 2012
Monoclonal antibodies for the S2 subunit of spike of SARS-CoV-1 cross-react with the newly-emerged SARS-CoV-2 Zheng, Zhiqiang; Monteil, Vanessa Marthe; Maurer-Stroh, Sebastian Eurosurveillance, Vol. 25, Issue 28 https://doi.org/10.2807/1560-7917.ES.2020.25.28.2000291	journal	July 2020
A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w Cingolani, Pablo; Platts, Adrian; Wang, Le Lily Fly, Vol. 6, Issue 2 https://doi.org/10.4161/fly.19695	journal	April 2012
Escape from neutralizing antibodies by SARS-CoV-2 spike protein variants Weisblum, Yiska; Schmidt, Fabian; Zhang, Fengwen eLife, Vol. 9 https://doi.org/10.7554/eLife.61312	journal	October 2020

Similar Records

Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity

Journal Article · Fri Jul 20 04:00:00 UTC 2007 · Biochemical and Biophysical Research Communications · OSTI ID:20991450

Du, Lanying; Kao, Richard Y; Zhou, Yusen; +7 more

Furin cleavage of the SARS coronavirus spike glycoprotein enhances cell-cell fusion but does not affect virion entry

Journal Article · Wed Jul 05 04:00:00 UTC 2006 · Virology · OSTI ID:20850527

Follis, Kathryn E; York, Joanne; Nunberg, Jack H

SARS-coronavirus spike S2 domain flanked by cysteine residues C822 and C833 is important for activation of membrane fusion

Journal Article · Sun Oct 25 04:00:00 UTC 2009 · Virology · OSTI ID:21357558

Madu, Ikenna G; Belouzard, Sandrine

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Broad betacoronavirus neutralization by a stem helix–specific human antibody

Citation Formats

References (79)

Similar Records

Related Subjects