Mixed Acid Fermentation of Carbohydrate-Rich Dairy Manure Hydrolysate
Abstract
Dairy manure (DM) is an abundant agricultural residue that is largely composed of lignocellulosic biomass. The aim of this study was to investigate if carbon derived from DM fibers can be recovered as medium-chain fatty acids (MCFAs), which are mixed culture fermentation products of economic interest. DM fibers were subjected to combinations of physical, enzymatic, chemical, and thermochemical pretreatments to evaluate the possibility of producing carbohydrate-rich hydrolysates suitable for microbial fermentation by mixed cultures. Among the pretreatments tested, decrystalization dilute acid pretreatment (DCDA) produced the highest concentrations of glucose and xylose, and was selected for further experiments. Bioreactors fed DCDA hydrolysate were operated. Acetic acid and butyric acid comprised the majority of end products during operation of the bioreactors. MCFAs were transiently produced at a maximum concentration of 0.17 mg COD MCFAs /mg COD Total . Analyses of the microbial communities in the bioreactors suggest that lactic acid bacteria, Megasphaera , and Caproiciproducens were involved in MCFA and C4 production during DCDA hydrolysate metabolism.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Institute of Food and Agriculture; Univ. of Wisconsin-Madison; Great Lakes Bioenergy Research Center
- OSTI Identifier:
- 1811718
- Alternate Identifier(s):
- OSTI ID: 1852643
- Grant/Contract Number:
- SC0018409; 2017-67003-26055
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Bioengineering and Biotechnology
- Additional Journal Information:
- Journal Name: Frontiers in Bioengineering and Biotechnology Journal Volume: 9; Journal ID: ISSN 2296-4185
- Publisher:
- Frontiers Media SA
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; dairy manure; biomass pretreatment; medium-chain fatty acids; mixed culture fermentation; lignocellulosic biomass
Citation Formats
Ingle, Abel T., Fortney, Nathaniel W., Walters, Kevin A., Donohue, Timothy J., and Noguera, Daniel R. Mixed Acid Fermentation of Carbohydrate-Rich Dairy Manure Hydrolysate. Switzerland: N. p., 2021.
Web. doi:10.3389/fbioe.2021.724304.
Ingle, Abel T., Fortney, Nathaniel W., Walters, Kevin A., Donohue, Timothy J., & Noguera, Daniel R. Mixed Acid Fermentation of Carbohydrate-Rich Dairy Manure Hydrolysate. Switzerland. https://doi.org/10.3389/fbioe.2021.724304
Ingle, Abel T., Fortney, Nathaniel W., Walters, Kevin A., Donohue, Timothy J., and Noguera, Daniel R. Tue .
"Mixed Acid Fermentation of Carbohydrate-Rich Dairy Manure Hydrolysate". Switzerland. https://doi.org/10.3389/fbioe.2021.724304.
@article{osti_1811718,
title = {Mixed Acid Fermentation of Carbohydrate-Rich Dairy Manure Hydrolysate},
author = {Ingle, Abel T. and Fortney, Nathaniel W. and Walters, Kevin A. and Donohue, Timothy J. and Noguera, Daniel R.},
abstractNote = {Dairy manure (DM) is an abundant agricultural residue that is largely composed of lignocellulosic biomass. The aim of this study was to investigate if carbon derived from DM fibers can be recovered as medium-chain fatty acids (MCFAs), which are mixed culture fermentation products of economic interest. DM fibers were subjected to combinations of physical, enzymatic, chemical, and thermochemical pretreatments to evaluate the possibility of producing carbohydrate-rich hydrolysates suitable for microbial fermentation by mixed cultures. Among the pretreatments tested, decrystalization dilute acid pretreatment (DCDA) produced the highest concentrations of glucose and xylose, and was selected for further experiments. Bioreactors fed DCDA hydrolysate were operated. Acetic acid and butyric acid comprised the majority of end products during operation of the bioreactors. MCFAs were transiently produced at a maximum concentration of 0.17 mg COD MCFAs /mg COD Total . Analyses of the microbial communities in the bioreactors suggest that lactic acid bacteria, Megasphaera , and Caproiciproducens were involved in MCFA and C4 production during DCDA hydrolysate metabolism.},
doi = {10.3389/fbioe.2021.724304},
journal = {Frontiers in Bioengineering and Biotechnology},
number = ,
volume = 9,
place = {Switzerland},
year = {Tue Aug 03 00:00:00 EDT 2021},
month = {Tue Aug 03 00:00:00 EDT 2021}
}
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