Expanding Molecular Coverage in Mass Spectrometry Imaging of Microbial Systems Using Metal-Assisted Laser Desorption/Ionization

Lukowski, Jessica K.; Bhattacharjee, Arunima; Yannarell, Sarah M.; Schwarz, Kaitlyn; Shor, Leslie M.; Shank, Elizabeth A.; Anderton, Christopher R.; Gralnick, ed., Jeffrey A.

doi:10.1128/Spectrum.00520-21

Title: Expanding Molecular Coverage in Mass Spectrometry Imaging of Microbial Systems Using Metal-Assisted Laser Desorption/Ionization

Journal Article · 02 September 2021 · Microbiology Spectrum

DOI: https://doi.org/10.1128/Spectrum.00520-21 · OSTI ID:1808916

Lukowski, Jessica K. ^[1]; Bhattacharjee, Arunima ^[1];

^[2]; Schwarz, Kaitlyn ^[1]; Shor, Leslie M. ^[3];

^[4];

^[1]; Gralnick, ed., Jeffrey A.

Environmental Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA
University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
University of Connecticut, Storrs, Connecticut, USA
University of Massachusetts Medical School, Worcester, Massachusetts, USA

Matrix-assisted laser desorption ionization mass spectrometry imaging is becoming an important technique to investigate molecular processes within microbial colonies and microbiomes under different environmental conditions. However, this method is limited in terms of the types and classes of molecules that can be detected.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; SC0014664; SC0019012

OSTI ID:: 1808916

Report Number(s):: PNNL-SA--162394; e00520-21

Journal Information:: Microbiology Spectrum, Journal Name: Microbiology Spectrum Journal Issue: 1 Vol. 9; ISSN 2165-0497

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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