Deinococcus radiodurans UWO298 Dependence on Background Radiation for Optimal Growth
Abstract
Ionizing radiation is a major environmental variable for cells on Earth, and so organisms have adapted to either prevent or to repair damages caused by it, primarily from the appearance and accumulation of reactive oxygen species (ROS). In this study, we measured the differential gene expression in Deinococcus radiodurans UWO298 cultures deprived of background ionizing radiation (IR) while growing 605 m underground at the Waste Isolation Pilot Plant (WIPP), reducing the dose rate from 72.1 to 0.9 nGy h –1 from control to treatment, respectively. This reduction in IR dose rate delayed the entry into the exponential phase of the IR-shielded cultures, resulting in a lower biomass accumulation for the duration of the experiment. The RNASeq-based transcriptome analysis showed the differential expression of 0.2 and 2.7% of the D. radiodurans genome after 24 and 34 h of growth in liquid culture, respectively. Gene expression regulation after 34 h was characterized by the downregulation of genes involved in folding newly synthesized and denatured/misfolded proteins, in the assimilation of nitrogen for amino acid synthesis and in the control of copper transport and homeostasis to prevent oxidative stress. We also observed the upregulation of genes coding for proteins with transport and cell wallmore »
- Authors:
- Publication Date:
- Research Org.:
- New Mexico State Univ., Las Cruces, NM (United States)
- Sponsoring Org.:
- USDOE Office of Environmental Management (EM)
- OSTI Identifier:
- 1781937
- Alternate Identifier(s):
- OSTI ID: 1848953
- Grant/Contract Number:
- EM0002423
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Genetics
- Additional Journal Information:
- Journal Name: Frontiers in Genetics Journal Volume: 12; Journal ID: ISSN 1664-8021
- Publisher:
- Frontiers Media SA
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Genetics & Heredity; background radiation; transcriptome analysis; stress response; homeostatic control; de novo transcriptome analysis
Citation Formats
Castillo, Hugo, Li, Xiaoping, and Smith, Geoffrey B. Deinococcus radiodurans UWO298 Dependence on Background Radiation for Optimal Growth. Switzerland: N. p., 2021.
Web. doi:10.3389/fgene.2021.644292.
Castillo, Hugo, Li, Xiaoping, & Smith, Geoffrey B. Deinococcus radiodurans UWO298 Dependence on Background Radiation for Optimal Growth. Switzerland. https://doi.org/10.3389/fgene.2021.644292
Castillo, Hugo, Li, Xiaoping, and Smith, Geoffrey B. Thu .
"Deinococcus radiodurans UWO298 Dependence on Background Radiation for Optimal Growth". Switzerland. https://doi.org/10.3389/fgene.2021.644292.
@article{osti_1781937,
title = {Deinococcus radiodurans UWO298 Dependence on Background Radiation for Optimal Growth},
author = {Castillo, Hugo and Li, Xiaoping and Smith, Geoffrey B.},
abstractNote = {Ionizing radiation is a major environmental variable for cells on Earth, and so organisms have adapted to either prevent or to repair damages caused by it, primarily from the appearance and accumulation of reactive oxygen species (ROS). In this study, we measured the differential gene expression in Deinococcus radiodurans UWO298 cultures deprived of background ionizing radiation (IR) while growing 605 m underground at the Waste Isolation Pilot Plant (WIPP), reducing the dose rate from 72.1 to 0.9 nGy h –1 from control to treatment, respectively. This reduction in IR dose rate delayed the entry into the exponential phase of the IR-shielded cultures, resulting in a lower biomass accumulation for the duration of the experiment. The RNASeq-based transcriptome analysis showed the differential expression of 0.2 and 2.7% of the D. radiodurans genome after 24 and 34 h of growth in liquid culture, respectively. Gene expression regulation after 34 h was characterized by the downregulation of genes involved in folding newly synthesized and denatured/misfolded proteins, in the assimilation of nitrogen for amino acid synthesis and in the control of copper transport and homeostasis to prevent oxidative stress. We also observed the upregulation of genes coding for proteins with transport and cell wall assembly roles. These results show that D. radiodurans is sensitive to the absence of background levels of ionizing radiation and suggest that its transcriptional response is insufficient to maintain optimal growth.},
doi = {10.3389/fgene.2021.644292},
journal = {Frontiers in Genetics},
number = ,
volume = 12,
place = {Switzerland},
year = {Thu May 06 00:00:00 EDT 2021},
month = {Thu May 06 00:00:00 EDT 2021}
}
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