Nanoparticle-Mediated Assembly of Peptoid Nanosheets Functionalized with Solid-Binding Proteins: Designing Heterostructures for Hierarchy

Ma, Jinrong; Cai, Bin; Zhang, Shuai; Jian, Tengyue; De Yoreo, James J.; Chen, Chun-Long; Baneyx, François

doi:10.1021/acs.nanolett.0c04285

Title: Nanoparticle-Mediated Assembly of Peptoid Nanosheets Functionalized with Solid-Binding Proteins: Designing Heterostructures for Hierarchy

Journal Article · Mon Feb 08 00:00:00 UTC 2021 · Nano Letters

DOI: https://doi.org/10.1021/acs.nanolett.0c04285 · OSTI ID:1778838

Ma, Jinrong ^[1]; Cai, Bin ^[2];

^[2]; Jian, Tengyue ^[2];

^[2];

^[1]

Univ. of Washington, Seattle, WA (United States). Molecular Engineering and Sciences Institute
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

The fabrication of ordered architectures that intimately integrate polymer, protein and inorganic components remains difficult. Two promising building blocks to tackle this challenge are peptoids, peptide mimics capable of self-assembly into well-defined structures, and solid-binding peptides, which offer a biological path to controlled inorganic assembly. Here, we report on the synthesis of 3.3 nm-thick, thiol-reactive peptoid nanosheets from equimolar mixtures of unmodified and maleimide-derivatized versions of the Nbpe₆Nce₆ oligomer, optimize the location of engineered cysteine residues in silica-binding derivatives of superfolder green fluorescent protein for maleimide conjugation, and react the two components to form proteinpeptoid hybrids exhibiting partial or uniform protein coverage on both of their sides. Using 10 nm silica nanoparticles, we trigger the stacking of these 2D structures into a multi-layer material comprised of alternating peptoid, protein and organic layers. This simple and modular approach to hierarchical hybrid synthesis should prove useful in bioimaging and photocatalysis applications.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Univ. of Washington, Seattle, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-76RL01830; SC0019288

OSTI ID:: 1778838

Report Number(s):: PNNL-SA--159754

Journal Information:: Nano Letters, Journal Name: Nano Letters Journal Issue: 4 Vol. 21; ISSN 1530-6984

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Nanoparticle-Mediated Assembly of Peptoid Nanosheets Functionalized with Solid-Binding Proteins: Designing Heterostructures for Hierarchy

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