Synthetic biology of polyketide synthases

Yuzawa, Satoshi; Backman, Tyler W. H.; Keasling, Jay D.; Katz, Leonard

doi:10.1007/s10295-018-2021-9

Title: Synthetic biology of polyketide synthases

Journal Article · Sun Jul 01 00:00:00 UTC 2018 · Journal of Industrial Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s10295-018-2021-9 · OSTI ID:1773512

Yuzawa, Satoshi ^[1]; Backman, Tyler W. H. ^[2]; Keasling, Jay D. ^[3]; Katz, Leonard ^[4]

0000 0001 2231 4551 grid.184769.5 Biological Systems and Engineering Division Lawrence Berkeley National Laboratory 94720 Berkeley CA USA, 0000 0004 0407 8980 grid.451372.6 Joint BioEnergy Institute 94608 Emeryville CA USA
0000 0001 2231 4551 grid.184769.5 Biological Systems and Engineering Division Lawrence Berkeley National Laboratory 94720 Berkeley CA USA, 0000 0004 0407 8980 grid.451372.6 Joint BioEnergy Institute 94608 Emeryville CA USA, 0000 0001 2181 7878 grid.47840.3f QB3 Institute University of California 94720 Berkeley CA USA
0000 0001 2231 4551 grid.184769.5 Biological Systems and Engineering Division Lawrence Berkeley National Laboratory 94720 Berkeley CA USA; 0000 0004 0407 8980 grid.451372.6 Joint BioEnergy Institute 94608 Emeryville CA USA; 0000 0001 2181 7878 grid.47840.3f QB3 Institute University of California 94720 Berkeley CA USA; 0000 0001 2181 7878 grid.47840.3f Department of Bioengineering University of California 94720 Berkeley CA USA; 0000 0001 2181 7878 grid.47840.3f Department of Chemical and Biomolecular Engineering University of California 94720 Berkeley CA USA
0000 0004 0407 8980 grid.451372.6 Joint BioEnergy Institute 94608 Emeryville CA USA, 0000 0001 2181 7878 grid.47840.3f QB3 Institute University of California 94720 Berkeley CA USA

Abstract Complex reduced polyketides represent the largest class of natural products that have applications in medicine, agriculture, and animal health. This structurally diverse class of compounds shares a common methodology of biosynthesis employing modular enzyme systems called polyketide synthases (PKSs). The modules are composed of enzymatic domains that share sequence and functional similarity across all known PKSs. We have used the nomenclature of synthetic biology to classify the enzymatic domains and modules as parts and devices, respectively, and have generated detailed lists of both. In addition, we describe the chassis (hosts) that are used to assemble, express, and engineer the parts and devices to produce polyketides. We describe a recently developed software tool to design PKS system and provide an example of its use. Finally, we provide perspectives of what needs to be accomplished to fully realize the potential that synthetic biology approaches bring to this class of molecules.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1773512

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 7 Vol. 45; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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