Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

Cai, Guangqin; Wang, Geliang; Kim, Sang-Chul; Li, Jianwu; Zhou, Yongming; Wang, Xuemin

doi:10.1186/s13068-021-01899-w

Title: Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

Abstract

Abstract Background Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results Here, we examined the effect of two lipid transporters, FAX1 ( fatty acid export1 ) and ABCA9 ( ATP-binding cassette transporter subfamily A9 ) on oil production and lipid metabolism in the oilseed plant Camelina sativa . Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1 -OEs and ABCA9 -OEs increasing seed length and width, respectively, whereas FAX1 / ABCA9 -OEs increasing both. FAX1- OE and ABCA9- OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2 , PDAT1 , and LEC1 , was increased in developing seeds of FAX1 - and/or ABCA9 -OEs. Conclusion These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.

Authors:

Cai, Guangqin; Wang, Geliang; Kim, Sang-Chul; Li, Jianwu; Zhou, Yongming;

Publication Date:: Sat Feb 27 00:00:00 EST 2021

Research Org.:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); National Natural Science Foundation of China (NSFC); National Key Research and Development Program of China; Office of China Postdoctoral Council

OSTI Identifier:: 1768103

Alternate Identifier(s):: OSTI ID: 1848177

Grant/Contract Number:: AR0000202; 31801029; 2016YFD0100506; 2017YFE0104800; 20160034

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 14 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; biotechnology & applied microbiology; energy & fuels; camelina; lipid metabolism; oil production; seed weight; transporters

Citation Formats


                    Cai, Guangqin, Wang, Geliang, Kim, Sang-Chul, Li, Jianwu, Zhou, Yongming, and Wang, Xuemin. Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina.  Netherlands: N. p., 2021. 
Web.  doi:10.1186/s13068-021-01899-w.

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                    Cai, Guangqin, Wang, Geliang, Kim, Sang-Chul, Li, Jianwu, Zhou, Yongming, & Wang, Xuemin. Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina.  Netherlands.  https://doi.org/10.1186/s13068-021-01899-w

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                    Cai, Guangqin, Wang, Geliang, Kim, Sang-Chul, Li, Jianwu, Zhou, Yongming, and Wang, Xuemin. Sat .  
"Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina".  Netherlands.  https://doi.org/10.1186/s13068-021-01899-w.

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@article{osti_1768103,

  title        = {Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina},

  author       = {Cai, Guangqin and Wang, Geliang and Kim, Sang-Chul and Li, Jianwu and Zhou, Yongming and Wang, Xuemin},

  abstractNote = {Abstract Background Lipid transporters play an essential role in lipid delivery and distribution, but their influence on seed oil production in oilseed crops is not well studied. Results Here, we examined the effect of two lipid transporters, FAX1 ( fatty acid export1 ) and ABCA9 ( ATP-binding cassette transporter subfamily A9 ) on oil production and lipid metabolism in the oilseed plant Camelina sativa . Overexpression (OE) of FAX1 and ABCA9 increased seed weight and size, with FAX1 -OEs and ABCA9 -OEs increasing seed length and width, respectively, whereas FAX1 / ABCA9 -OEs increasing both. FAX1- OE and ABCA9- OE displayed additive effects on seed oil content and seed yield. Also, OE of FAX1 and ABCA9 affected membrane lipid composition in developing pods, especially on phosphatidylcholine, phosphatidylethanolamine, and phosphatidylglycerol. The expression of some genes involved in seed oil synthesis, such as DGAT2 , PDAT1 , and LEC1 , was increased in developing seeds of FAX1 - and/or ABCA9 -OEs. Conclusion These results indicate that increased expression of FAX1 and ABCA9 can potentially be applied to improving camelina oil production.},

  doi          = {10.1186/s13068-021-01899-w},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 14,

  place        = {Netherlands},

  year         = {Sat Feb 27 00:00:00 EST 2021},

  month        = {Sat Feb 27 00:00:00 EST 2021}

}

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Title: Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina

Abstract

Citation Formats

Redirection of metabolic flux for high levels of omega-7 monounsaturated fatty acid accumulation in camelina seeds journal, July 2014

Camelina uses, genetics, genomics, production, and management journal, December 2016

Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants journal, March 2020

High level accumulation of EPA and DHA in field‐grown transgenic Camelina – a multi‐territory evaluation of TAG accumulation and heterogeneity journal, May 2020

Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds journal, November 2014

GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein journal, February 2006

The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure journal, April 2014

Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis journal, March 2011

Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop journal, December 2013

Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil journal, January 2018

Deletion in a gene associated with grain size increased yields during rice domestication journal, July 2008

Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa journal, February 2018

AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum journal, December 2012

Synthesis of oleyl oleate wax esters in Arabidopsis thaliana and Camelina sativa seed oil journal, April 2015

Camelina seed transcriptome: a tool for meal and oil improvement and translational research journal, April 2013

Transcriptional Regulation of Lipid Catabolism during Seedling Establishment journal, July 2020

Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight journal, November 2008

Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing journal, January 2017

FAX1, a Novel Membrane Protein Mediating Plastid Fatty Acid Export journal, February 2015

Field trial evaluation of the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Camelina sativa: Making fish oil substitutes in plants journal, December 2015

Genetic dissection of plant architecture and yield-related traits in Brassica napus journal, February 2016

Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA journal, January 2014

Metabolic Engineering of Fatty Acid Biosynthesis in Plants journal, January 2002

Oil-seed crop: Camelina sativa journal, May 1997

Transcriptome profiling of Camelina sativa to identify genes involved in triacylglycerol biosynthesis and accumulation in the developing seeds journal, July 2016

Natural variation in GS5 plays an important role in regulating grain size and yield in rice journal, October 2011

Plant acyl-CoA-binding proteins: An emerging family involved in plant development and stress responses journal, July 2016

Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation journal, September 2007

Genetic and Molecular Bases of Rice Yield journal, June 2010

Seed-specific overexpression of AtFAX1 increases seed oil content in Arabidopsis journal, June 2018

Some compositional properties of camelina (camelina sativa L. Crantz) seeds and oils journal, March 1995

Biodiesel from alternative oilseed feedstocks: camelina and field pennycress journal, March 2012

Diseases of Camelina sativa (false flax) journal, December 2009

A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase journal, April 2007

Control of grain size, shape and quality by OsSPL16 in rice journal, June 2012

Enhancing micro RNA 167A expression in seed decreases the α‐linolenic acid content and increases seed size in Camelina sativa journal, February 2019

Acyl-Lipid Metabolism journal, January 2013

An alternative pathway for the effective production of the omega-3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds journal, January 2015

Profiling Membrane Lipids in Plant Stress Responses: ROLE OF PHOSPHOLIPASE Dα IN FREEZING-INDUCED LIPID CHANGES IN journal, June 2002

Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value journal, March 2015

The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3 journal, November 2012

Overexpression of patatin-related phospholipase AIIIδ altered plant growth and increased seed oil content in camelina journal, December 2014

Redirection of metabolic flux for high levels of omega-7 monounsaturated fatty acid accumulation in camelina seeds
journal, July 2014

Camelina uses, genetics, genomics, production, and management
journal, December 2016

Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants
journal, March 2020

High level accumulation of EPA and DHA in field‐grown transgenic Camelina – a multi‐territory evaluation of TAG accumulation and heterogeneity
journal, May 2020

Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds
journal, November 2014

GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein
journal, February 2006

The emerging biofuel crop Camelina sativa retains a highly undifferentiated hexaploid genome structure
journal, April 2014

Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis
journal, March 2011

Successful high-level accumulation of fish oil omega-3 long-chain polyunsaturated fatty acids in a transgenic oilseed crop
journal, December 2013

Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil
journal, January 2018

Deletion in a gene associated with grain size increased yields during rice domestication
journal, July 2008

Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa
journal, February 2018

AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum
journal, December 2012

Synthesis of oleyl oleate wax esters in Arabidopsis thaliana and Camelina sativa seed oil
journal, April 2015

Camelina seed transcriptome: a tool for meal and oil improvement and translational research
journal, April 2013

Transcriptional Regulation of Lipid Catabolism during Seedling Establishment
journal, July 2020

Isolation and initial characterization of GW5, a major QTL associated with rice grain width and weight
journal, November 2008

Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing
journal, January 2017

FAX1, a Novel Membrane Protein Mediating Plastid Fatty Acid Export
journal, February 2015

Field trial evaluation of the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Camelina sativa: Making fish oil substitutes in plants
journal, December 2015

Genetic dissection of plant architecture and yield-related traits in Brassica napus
journal, February 2016

Metabolic Engineering Camelina sativa with Fish Oil-Like Levels of DHA
journal, January 2014

Metabolic Engineering of Fatty Acid Biosynthesis in Plants
journal, January 2002

Oil-seed crop: Camelina sativa
journal, May 1997

Transcriptome profiling of Camelina sativa to identify genes involved in triacylglycerol biosynthesis and accumulation in the developing seeds
journal, July 2016

Natural variation in GS5 plays an important role in regulating grain size and yield in rice
journal, October 2011

Plant acyl-CoA-binding proteins: An emerging family involved in plant development and stress responses
journal, July 2016

Generation of transgenic plants of a potential oilseed crop Camelina sativa by Agrobacterium-mediated transformation
journal, September 2007

Genetic and Molecular Bases of Rice Yield
journal, June 2010

Seed-specific overexpression of AtFAX1 increases seed oil content in Arabidopsis
journal, June 2018

Some compositional properties of camelina (camelina sativa L. Crantz) seeds and oils
journal, March 1995

Biodiesel from alternative oilseed feedstocks: camelina and field pennycress
journal, March 2012

Diseases of Camelina sativa (false flax)
journal, December 2009

A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase
journal, April 2007

Control of grain size, shape and quality by OsSPL16 in rice
journal, June 2012

Enhancing micro RNA 167A expression in seed decreases the α‐linolenic acid content and increases seed size in Camelina sativa
journal, February 2019

Acyl-Lipid Metabolism
journal, January 2013

An alternative pathway for the effective production of the omega-3 long-chain polyunsaturates EPA and ETA in transgenic oilseeds
journal, January 2015

Profiling Membrane Lipids in Plant Stress Responses: ROLE OF PHOSPHOLIPASE Dα IN FREEZING-INDUCED LIPID CHANGES IN
journal, June 2002

Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value
journal, March 2015

The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3
journal, November 2012

Overexpression of patatin-related phospholipase AIIIδ altered plant growth and increased seed oil content in camelina
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