The success of particular Acinetobacter baumannii clones: accumulating resistance and virulence inside a sugary shield
Abstract
Abstract Background In Portugal, carbapenem-resistant Acinetobacter baumannii (CRAB) has been associated with ST98, ST103 and ST208 (Oxford Scheme, Oxf) and a clone has usually been associated with a particular period of time. These clonal shifts were primarily explained by an increased antimicrobial resistance profile. Here we explore genomic and biochemical differences among these and more recent clones, which could further explain the diversity and evolution of this species. Methods A total of 116 CRAB isolates (2010–15), together with representatives of a previously described CRAB collection (4 isolates, 2001–06) were characterized by attenuated total reflection Fourier transform infrared spectroscopy (FTIR-ATR) and MLST. Representatives of different FTIR-ATR/MLST clusters were selected for WGS (n = 13), which allowed the in silico extraction of resistance and virulence genes, capsule locus and SNP analysis. Results A. baumannii clonal shifts of OXA-58-producing ST103Oxf (2001–04), OXA-40-producing ST98Oxf (2002–06), OXA-23-producing ST208Oxf (2006–10) and OXA-23-producing ST218Oxf (2010–15) were accompanied by an increase in AMR genes and virulence factors. FTIR-ATR clustering was congruent with sugar composition predicted from the capsular locus: a fucosamine cluster comprising ST98Oxf, ST103Oxf and a single ST218Oxf isolate; a pseudaminic acid cluster of ST208Oxf and ST1557Oxf isolates; and legionaminic acid, resembling the sialic acid from mammalian cells, inmore »
- Authors:
-
- UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal, ESALD, Instituto Politécnico de Castelo Branco, Castelo Branco, Portugal
- UCIBIO/REQUIMTE, Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal
- Hospital Geral de Santo António (HGSA), Porto, Portugal
- Publication Date:
- Sponsoring Org.:
- USDOE Office of Nuclear Energy (NE), Nuclear Fuel Cycle and Supply Chain
- OSTI Identifier:
- 1761230
- Grant/Contract Number:
- SFRH/BD/88028/2012; SFRH/BD/132497/2017; DL57/2016/CP1346/CT0034
- Resource Type:
- Published Article
- Journal Name:
- Journal of Antimicrobial Chemotherapy
- Additional Journal Information:
- Journal Name: Journal of Antimicrobial Chemotherapy Journal Volume: 76 Journal Issue: 2; Journal ID: ISSN 0305-7453
- Publisher:
- Oxford University Press
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Silva, Liliana, Grosso, Filipa, Rodrigues, Carla, Ksiezarek, Magdalena, Ramos, Helena, and Peixe, Luísa. The success of particular Acinetobacter baumannii clones: accumulating resistance and virulence inside a sugary shield. United Kingdom: N. p., 2020.
Web. doi:10.1093/jac/dkaa453.
Silva, Liliana, Grosso, Filipa, Rodrigues, Carla, Ksiezarek, Magdalena, Ramos, Helena, & Peixe, Luísa. The success of particular Acinetobacter baumannii clones: accumulating resistance and virulence inside a sugary shield. United Kingdom. https://doi.org/10.1093/jac/dkaa453
Silva, Liliana, Grosso, Filipa, Rodrigues, Carla, Ksiezarek, Magdalena, Ramos, Helena, and Peixe, Luísa. Thu .
"The success of particular Acinetobacter baumannii clones: accumulating resistance and virulence inside a sugary shield". United Kingdom. https://doi.org/10.1093/jac/dkaa453.
@article{osti_1761230,
title = {The success of particular Acinetobacter baumannii clones: accumulating resistance and virulence inside a sugary shield},
author = {Silva, Liliana and Grosso, Filipa and Rodrigues, Carla and Ksiezarek, Magdalena and Ramos, Helena and Peixe, Luísa},
abstractNote = {Abstract Background In Portugal, carbapenem-resistant Acinetobacter baumannii (CRAB) has been associated with ST98, ST103 and ST208 (Oxford Scheme, Oxf) and a clone has usually been associated with a particular period of time. These clonal shifts were primarily explained by an increased antimicrobial resistance profile. Here we explore genomic and biochemical differences among these and more recent clones, which could further explain the diversity and evolution of this species. Methods A total of 116 CRAB isolates (2010–15), together with representatives of a previously described CRAB collection (4 isolates, 2001–06) were characterized by attenuated total reflection Fourier transform infrared spectroscopy (FTIR-ATR) and MLST. Representatives of different FTIR-ATR/MLST clusters were selected for WGS (n = 13), which allowed the in silico extraction of resistance and virulence genes, capsule locus and SNP analysis. Results A. baumannii clonal shifts of OXA-58-producing ST103Oxf (2001–04), OXA-40-producing ST98Oxf (2002–06), OXA-23-producing ST208Oxf (2006–10) and OXA-23-producing ST218Oxf (2010–15) were accompanied by an increase in AMR genes and virulence factors. FTIR-ATR clustering was congruent with sugar composition predicted from the capsular locus: a fucosamine cluster comprising ST98Oxf, ST103Oxf and a single ST218Oxf isolate; a pseudaminic acid cluster of ST208Oxf and ST1557Oxf isolates; and legionaminic acid, resembling the sialic acid from mammalian cells, in a cluster comprising ST218Oxf isolates. The whole-genome phylogenetic tree was congruent with MLST, with isolates presenting 5–28 938 SNPs. ST208Oxf and ST218Oxf presented ∼1900 SNPs while ST103Oxf and ST1557Oxf showed a greater number of SNPs (∼28 000). Conclusions Clonal shifts of CRAB were promoted, in our country, by consecutive virulence and AMR gene pool enlargement, together with features increasing pathogen–host adaptation. Worldwide dominance of ST218Oxf is supported by the combination of high AMR and virulence levels.},
doi = {10.1093/jac/dkaa453},
journal = {Journal of Antimicrobial Chemotherapy},
number = 2,
volume = 76,
place = {United Kingdom},
year = {Thu Nov 05 00:00:00 EST 2020},
month = {Thu Nov 05 00:00:00 EST 2020}
}
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Works referenced in this record:
Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees
journal, April 2016
- Letunic, Ivica; Bork, Peer
- Nucleic Acids Research, Vol. 44, Issue W1
Structure of the K2 capsule associated with the KL2 gene cluster of Acinetobacter baumannii
journal, March 2014
- Kenyon, Johanna J.; Marzaioli, Alberto M.; Hall, Ruth M.
- Glycobiology, Vol. 24, Issue 6
A pseudaminic acid or a legionaminic acid derivative transferase is strain-specifically implicated in the general protein O-glycosylation system of the periodontal pathogen Tannerella forsythia
journal, March 2017
- Tomek, Markus B.; Janesch, Bettina; Maresch, Daniel
- Glycobiology, Vol. 27, Issue 6
5,7-di-N-acetyl-acinetaminic acid: A novel non-2-ulosonic acid found in the capsule of an Acinetobacter baumannii isolate
journal, January 2015
- Kenyon, Johanna J.; Marzaioli, Alberto M.; De Castro, Cristina
- Glycobiology, Vol. 25, Issue 6
Biology of Acinetobacter baumannii: Pathogenesis, Antibiotic Resistance Mechanisms, and Prospective Treatment Options
journal, March 2017
- Lee, Chang-Ro; Lee, Jung Hun; Park, Moonhee
- Frontiers in Cellular and Infection Microbiology, Vol. 7
Blue-Carba, an Easy Biochemical Test for Detection of Diverse Carbapenemase Producers Directly from Bacterial Cultures
journal, October 2013
- Pires, J.; Novais, A.; Peixe, L.
- Journal of Clinical Microbiology, Vol. 51, Issue 12
Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218
journal, April 2014
- Sousa, Clara; Silva, Liliana; Grosso, Filipa
- Journal of Photochemistry and Photobiology B: Biology, Vol. 133
Identification of carbapenem-resistant Acinetobacter baumannii clones using infrared spectroscopy: Carbapenem-resistant A. baumannii analysis with FTIR spectroscopy
journal, November 2012
- Sousa, Clara; Grosso, Filipa; Meirinhos-Soares, Luís
- Journal of Biophotonics, Vol. 7, Issue 5
Diversity in the Major Polysaccharide Antigen of Acinetobacter Baumannii Assessed by DNA Sequencing, and Development of a Molecular Serotyping Scheme
journal, July 2013
- Hu, Dalong; Liu, Bin; Dijkshoorn, Lenie
- PLoS ONE, Vol. 8, Issue 7
Uncovering the mechanisms of Acinetobacter baumannii virulence
journal, December 2017
- Harding, Christian M.; Hennon, Seth W.; Feldman, Mario F.
- Nature Reviews Microbiology, Vol. 16, Issue 2
Antibiotic Modulation of Capsular Exopolysaccharide and Virulence in Acinetobacter baumannii
journal, February 2015
- Geisinger, Edward; Isberg, Ralph R.
- PLOS Pathogens, Vol. 11, Issue 2
Fourier transform infrared spectroscopy: unlocking fundamentals and prospects for bacterial strain typing
journal, November 2018
- Novais, Ângela; Freitas, Ana R.; Rodrigues, Carla
- European Journal of Clinical Microbiology & Infectious Diseases, Vol. 38, Issue 3
A Front Line on Klebsiella pneumoniae Capsular Polysaccharide Knowledge: Fourier Transform Infrared Spectroscopy as an Accurate and Fast Typing Tool
journal, March 2020
- Rodrigues, Carla; Sousa, Clara; Lopes, João A.
- mSystems, Vol. 5, Issue 2
Long-term dissemination of an OXA-40 carbapenemase-producing Acinetobacter baumannii clone in the Iberian Peninsula
journal, June 2004
- Da Silva, G. J.
- Journal of Antimicrobial Chemotherapy, Vol. 54, Issue 1
Role of Common bla OXA-24/OXA-40 -Carrying Platforms and Plasmids in the Spread of OXA-24/OXA-40 among Acinetobacter Species Clinical Isolates
journal, April 2012
- Grosso, Filipa; Quinteira, Sandra; Poirel, Laurent
- Antimicrobial Agents and Chemotherapy, Vol. 56, Issue 7
Genome Sequence of a Dominant, Multidrug-Resistant Acinetobacter baumannii Strain, TCDC-AB0715
journal, March 2011
- Chen, C. -C.; Lin, Y. -C.; Sheng, W. -H.
- Journal of Bacteriology, Vol. 193, Issue 9
Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species
journal, July 2009
- Gundi, Vijay A. K. B.; Dijkshoorn, Lenie; Burignat, Sophie
- Microbiology, Vol. 155, Issue 7
Complete Genome Analysis of Three Acinetobacter baumannii Clinical Isolates in China for Insight into the Diversification of Drug Resistance Elements
journal, June 2013
- Zhu, Lingxiang; Yan, Zhongqiang; Zhang, Zhaojun
- PLoS ONE, Vol. 8, Issue 6
Solving the Problem of Comparing Whole Bacterial Genomes across Different Sequencing Platforms
journal, August 2014
- Kaas, Rolf S.; Leekitcharoenphon, Pimlapas; Aarestrup, Frank M.
- PLoS ONE, Vol. 9, Issue 8
Heme uptake and utilization by hypervirulent Acinetobacter baumannii LAC-4 is dependent on a canonical heme oxygenase (abHemO)
journal, September 2019
- Giardina, Bennett J.; Shahzad, Saba; Huang, Weiliang
- Archives of Biochemistry and Biophysics, Vol. 672
Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.
journal, April 2006
- Woodford, N.; Ellington, M.; Coelho, J.
- International Journal of Antimicrobial Agents, Vol. 27, Issue 4
Understanding the dynamics of imipenem-resistant Acinetobacter baumannii lineages within Portugal
journal, August 2011
- Grosso, F.; Quinteira, S.; Peixe, L.
- Clinical Microbiology and Infection, Vol. 17, Issue 8