Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production

Antoniewicz, Maciek R.

doi:10.1016/j.copbio.2019.07.001

Title: Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production

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Abstract

Methanol is an attractive and broadly available substrate for large-scale bioproduction of fuels and chemicals. It contains more energy and electrons per carbon than carbohydrates and can be cheaply produced from natural gas. Synthetic methylotrophy refers to the development of non-native methylotrophs such as Escherichia coli and Corynebacterium glutamicum to utilize methanol as a carbon source. Furthermore, we discuss recent advances in engineering these industrial hosts to assimilate methanol for growth and chemicals production through the introduction of the ribulose monophosphate (RuMP) cycle. In addition, we present novel strategies based on flux coupling and adaptive laboratory evolution to engineer new strains that can grow exclusively on methanol.

Authors:

^[1]

Univ. of Delaware, Newark, DE (United States)

Publication Date:: Mon Aug 19 00:00:00 EDT 2019

Research Org.:: Univ. of Delaware, Newark, DE (United States)

Sponsoring Org.:: USDOE Advanced Research Projects Agency - Energy (ARPA-E)

OSTI Identifier:: 1747974

Alternate Identifier(s):: OSTI ID: 1778468

Grant/Contract Number:: AR0000432

Resource Type:: Accepted Manuscript

Journal Name:: Current Opinion in Biotechnology

Additional Journal Information:: Journal Volume: 59; Journal ID: ISSN 0958-1669

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES

Citation Formats


                    Antoniewicz, Maciek R. Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production.  United States: N. p., 2019. 
Web.  doi:10.1016/j.copbio.2019.07.001.

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                    Antoniewicz, Maciek R. Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production.  United States.  https://doi.org/10.1016/j.copbio.2019.07.001

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                    Antoniewicz, Maciek R. Mon .  
"Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production".  United States.  https://doi.org/10.1016/j.copbio.2019.07.001.  https://www.osti.gov/servlets/purl/1747974.

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@article{osti_1747974,

  title        = {Synthetic methylotrophy: Strategies to assimilate methanol for growth and chemicals production},

  author       = {Antoniewicz, Maciek R.},

  abstractNote = {Methanol is an attractive and broadly available substrate for large-scale bioproduction of fuels and chemicals. It contains more energy and electrons per carbon than carbohydrates and can be cheaply produced from natural gas. Synthetic methylotrophy refers to the development of non-native methylotrophs such as Escherichia coli and Corynebacterium glutamicum to utilize methanol as a carbon source. Furthermore, we discuss recent advances in engineering these industrial hosts to assimilate methanol for growth and chemicals production through the introduction of the ribulose monophosphate (RuMP) cycle. In addition, we present novel strategies based on flux coupling and adaptive laboratory evolution to engineer new strains that can grow exclusively on methanol.},

  doi          = {10.1016/j.copbio.2019.07.001},

  journal      = {Current Opinion in Biotechnology},

  number       = ,

  volume       = 59,

  place        = {United States},

  year         = {Mon Aug 19 00:00:00 EDT 2019},

  month        = {Mon Aug 19 00:00:00 EDT 2019}

}

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