Diversity and Distribution of a Novel Genus of Hyperthermophilic Aquificae Viruses Encoding a Proof-Reading Family-A DNA Polymerase
Abstract
Despite the high abundance of Aquificae in many geothermal systems, these bacteria are difficult to culture and no viruses infecting members of this phylum have been isolated. Here, we describe the complete, circular dsDNA Uncultivated Virus Genome (UViG) of Thermocrinis Octopus Spring virus (TOSV), derived from metagenomic data, along with eight related UViGs representing three additional viral species. Despite low overall similarity among viruses from different hot springs, the genomes shared a high degree of synteny, and encoded numerous genes for nucleotide metabolism, including a PolA-type DNA polymerase polyprotein with likely accessory functions, a DNA Pol III sliding clamp, a thymidylate kinase, a DNA gyrase, a helicase, and a DNA methylase. Also present were conserved genes predicted to code for phage capsid, large and small subunits of terminase, portal protein, holin, and lytic transglycosylase, all consistent with a distant relatedness to cultivated Caudovirales. These viruses are predicted to infect Aquificae, as multiple CRISPR spacers matching the viral genomes were identified within the genomes and metagenomic contigs from these bacteria. Based on the predicted atypical bi-directional replication strategy, low sequence similarity to known viral genomes, and unique position in gene-sharing networks, we propose a new putative genus, “Pyrovirus,” in the ordermore »
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Nevada, Reno, NV (United States); Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1712540
- Alternate Identifier(s):
- OSTI ID: 1848391
- Grant/Contract Number:
- EE-0000716; EE0000716; AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Name: Frontiers in Microbiology Journal Volume: 11; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Media SA
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology; viral metagenomics; bacteriophage; uncultivated virus genome; UViG; geothermal spring; DNA polymerase
Citation Formats
Palmer, Marike, Hedlund, Brian P., Roux, Simon, Tsourkas, Philippos K., Doss, Ryan K., Stamereilers, Casey, Mehta, Astha, Dodsworth, Jeremy A., Lodes, Michael, Monsma, Scott, Glavina del Rio, Tijana, Schoenfeld, Thomas W., Eloe-Fadrosh, Emiley A., and Mead, David A. Diversity and Distribution of a Novel Genus of Hyperthermophilic Aquificae Viruses Encoding a Proof-Reading Family-A DNA Polymerase. Switzerland: N. p., 2020.
Web. doi:10.3389/fmicb.2020.583361.
Palmer, Marike, Hedlund, Brian P., Roux, Simon, Tsourkas, Philippos K., Doss, Ryan K., Stamereilers, Casey, Mehta, Astha, Dodsworth, Jeremy A., Lodes, Michael, Monsma, Scott, Glavina del Rio, Tijana, Schoenfeld, Thomas W., Eloe-Fadrosh, Emiley A., & Mead, David A. Diversity and Distribution of a Novel Genus of Hyperthermophilic Aquificae Viruses Encoding a Proof-Reading Family-A DNA Polymerase. Switzerland. https://doi.org/10.3389/fmicb.2020.583361
Palmer, Marike, Hedlund, Brian P., Roux, Simon, Tsourkas, Philippos K., Doss, Ryan K., Stamereilers, Casey, Mehta, Astha, Dodsworth, Jeremy A., Lodes, Michael, Monsma, Scott, Glavina del Rio, Tijana, Schoenfeld, Thomas W., Eloe-Fadrosh, Emiley A., and Mead, David A. Thu .
"Diversity and Distribution of a Novel Genus of Hyperthermophilic Aquificae Viruses Encoding a Proof-Reading Family-A DNA Polymerase". Switzerland. https://doi.org/10.3389/fmicb.2020.583361.
@article{osti_1712540,
title = {Diversity and Distribution of a Novel Genus of Hyperthermophilic Aquificae Viruses Encoding a Proof-Reading Family-A DNA Polymerase},
author = {Palmer, Marike and Hedlund, Brian P. and Roux, Simon and Tsourkas, Philippos K. and Doss, Ryan K. and Stamereilers, Casey and Mehta, Astha and Dodsworth, Jeremy A. and Lodes, Michael and Monsma, Scott and Glavina del Rio, Tijana and Schoenfeld, Thomas W. and Eloe-Fadrosh, Emiley A. and Mead, David A.},
abstractNote = {Despite the high abundance of Aquificae in many geothermal systems, these bacteria are difficult to culture and no viruses infecting members of this phylum have been isolated. Here, we describe the complete, circular dsDNA Uncultivated Virus Genome (UViG) of Thermocrinis Octopus Spring virus (TOSV), derived from metagenomic data, along with eight related UViGs representing three additional viral species. Despite low overall similarity among viruses from different hot springs, the genomes shared a high degree of synteny, and encoded numerous genes for nucleotide metabolism, including a PolA-type DNA polymerase polyprotein with likely accessory functions, a DNA Pol III sliding clamp, a thymidylate kinase, a DNA gyrase, a helicase, and a DNA methylase. Also present were conserved genes predicted to code for phage capsid, large and small subunits of terminase, portal protein, holin, and lytic transglycosylase, all consistent with a distant relatedness to cultivated Caudovirales. These viruses are predicted to infect Aquificae, as multiple CRISPR spacers matching the viral genomes were identified within the genomes and metagenomic contigs from these bacteria. Based on the predicted atypical bi-directional replication strategy, low sequence similarity to known viral genomes, and unique position in gene-sharing networks, we propose a new putative genus, “Pyrovirus,” in the order Caudovirales.},
doi = {10.3389/fmicb.2020.583361},
journal = {Frontiers in Microbiology},
number = ,
volume = 11,
place = {Switzerland},
year = {Thu Nov 12 00:00:00 EST 2020},
month = {Thu Nov 12 00:00:00 EST 2020}
}
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