Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis
Abstract
Abstract Background The production of methane by methanogens is dependent on numerous iron-sulfur (Fe-S) cluster proteins; yet, the machinery involved in Fe-S cluster biogenesis in methanogens remains largely unknown. Methanogen genomes encode uncharacterized homologs of the core components of the ISC (IscS and IscU) and SUF (SufBC) Fe-S cluster biogenesis systems found in bacteria and eukaryotes. Methanosarcina acetivorans contains three iscSU and two sufCB gene clusters. Here, we report genetic and biochemical characterization of M. acetivorans iscSU2 . Results Purified IscS2 exhibited pyridoxal 5′- phosphate-dependent release of sulfur from L-cysteine. Incubation of purified IscU2 with IscS2, cysteine, and iron (Fe 2+ ) resulted in the formation of [4Fe-4S] clusters in IscU2. IscU2 transferred a [4Fe-4S] cluster to purified M. acetivorans apo-aconitase. IscU2 also restored the aconitase activity in air-exposed M. acetivorans cell lysate. These biochemical results demonstrate that IscS2 is a cysteine desulfurase and that IscU2 is a Fe-S cluster scaffold. M. acetivorans strain DJL60 deleted of iscSU2 was generated to ascertain the in vivo importance of IscSU2. Strain DJL60 had Fe-S cluster content and growth similar to the parent strain but lower cysteine desulfurase activity. Strain DJL60 also had lower intracellular persulfide content compared to the parent strain whenmore »
- Publication Date:
- Research Org.:
- Univ. of Arkansas, Fayetteville, AR (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); National Aeronautics and Space Administration (NASA)
- OSTI Identifier:
- 1686195
- Alternate Identifier(s):
- OSTI ID: 1852947
- Grant/Contract Number:
- SC0019226; MCB1817819; NNX12AR60G
- Resource Type:
- Published Article
- Journal Name:
- BMC Microbiology
- Additional Journal Information:
- Journal Name: BMC Microbiology Journal Volume: 20 Journal Issue: 1; Journal ID: ISSN 1471-2180
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology
Citation Formats
Deere, Thomas M., Prakash, Divya, Lessner, Faith H., Duin, Evert C., and Lessner, Daniel J. Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis. United Kingdom: N. p., 2020.
Web. doi:10.1186/s12866-020-02014-z.
Deere, Thomas M., Prakash, Divya, Lessner, Faith H., Duin, Evert C., & Lessner, Daniel J. Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis. United Kingdom. https://doi.org/10.1186/s12866-020-02014-z
Deere, Thomas M., Prakash, Divya, Lessner, Faith H., Duin, Evert C., and Lessner, Daniel J. Fri .
"Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis". United Kingdom. https://doi.org/10.1186/s12866-020-02014-z.
@article{osti_1686195,
title = {Methanosarcina acetivorans contains a functional ISC system for iron-sulfur cluster biogenesis},
author = {Deere, Thomas M. and Prakash, Divya and Lessner, Faith H. and Duin, Evert C. and Lessner, Daniel J.},
abstractNote = {Abstract Background The production of methane by methanogens is dependent on numerous iron-sulfur (Fe-S) cluster proteins; yet, the machinery involved in Fe-S cluster biogenesis in methanogens remains largely unknown. Methanogen genomes encode uncharacterized homologs of the core components of the ISC (IscS and IscU) and SUF (SufBC) Fe-S cluster biogenesis systems found in bacteria and eukaryotes. Methanosarcina acetivorans contains three iscSU and two sufCB gene clusters. Here, we report genetic and biochemical characterization of M. acetivorans iscSU2 . Results Purified IscS2 exhibited pyridoxal 5′- phosphate-dependent release of sulfur from L-cysteine. Incubation of purified IscU2 with IscS2, cysteine, and iron (Fe 2+ ) resulted in the formation of [4Fe-4S] clusters in IscU2. IscU2 transferred a [4Fe-4S] cluster to purified M. acetivorans apo-aconitase. IscU2 also restored the aconitase activity in air-exposed M. acetivorans cell lysate. These biochemical results demonstrate that IscS2 is a cysteine desulfurase and that IscU2 is a Fe-S cluster scaffold. M. acetivorans strain DJL60 deleted of iscSU2 was generated to ascertain the in vivo importance of IscSU2. Strain DJL60 had Fe-S cluster content and growth similar to the parent strain but lower cysteine desulfurase activity. Strain DJL60 also had lower intracellular persulfide content compared to the parent strain when cysteine was an exogenous sulfur source, linking IscSU2 to sulfur metabolism. Conclusions This study establishes that M. acetivorans contains functional IscS and IscU, the core components of the ISC Fe-S cluster biogenesis system and provides the first evidence that ISC operates in methanogens.},
doi = {10.1186/s12866-020-02014-z},
journal = {BMC Microbiology},
number = 1,
volume = 20,
place = {United Kingdom},
year = {Fri Oct 23 00:00:00 EDT 2020},
month = {Fri Oct 23 00:00:00 EDT 2020}
}
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