Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack [plus supplemental information]
Abstract
A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc2155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analyses, phage plating efficiency experiments, microscopy, and immunoprecipitation assays, we show that Butters genes located in the central region of the genome play a key role in the defense against heterotypic viral attack. Our study suggests that a two-component system, articulated by interactions between protein products of genes 30 and 31, confers defense against heterotypic phage infection by PurpleHaze (cluster A/subcluster A3) or Alma (cluster A/subcluster A9) but is insufficient to confer defense against attack by the heterotypic phage Island3 (cluster I/subcluster I1). Therefore, based on heterotypic phage plating efficiencies on the Butters lysogen, additional prophage genes required for defense are implicated and further show specificity of prophage-encoded defense systems. IMPORTANCE: Many sequenced bacterial genomes, including those of pathogenic bacteria, contain prophages. Some prophages encode defense systems that protect their bacterial host against heterotypic viral attack. Understanding the mechanisms undergirding these defense systems is crucial to appreciate the scope of bacterial immunity against viral infections and willmore »
- Authors:
-
[2]
- Lehigh Univ., Bethlehem, PA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Lehigh Univ., Bethlehem, PA (United States)
- Lehigh Univ., Bethlehem, PA (United States); Institute for Integrative Systems Biology (I2SysBio), CSIC-UV, Paterna, Valencia (Spain)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE; Pennsylvania Department of Community and Economic Development; Lehigh University
- OSTI Identifier:
- 1668358
- Report Number(s):
- SAND-2020-5059J
Journal ID: ISSN 2379-5077; 686079
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- mSystems
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 5; Related Information: This work was completed during graduate school and funded through numerous sources at Lehigh University; Journal ID: ISSN 2379-5077
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Mageeney, Catherine M., Mohammed, Hamidu T., Dies, Marta, Anbari, Samira, Cudkevich, Netta, Chen, Yanyan, Buceta, Javier, and Ware, Vassie C. Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack [plus supplemental information]. United States: N. p., 2020.
Web. doi:10.1128/msystems.00534-20.
Mageeney, Catherine M., Mohammed, Hamidu T., Dies, Marta, Anbari, Samira, Cudkevich, Netta, Chen, Yanyan, Buceta, Javier, & Ware, Vassie C. Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack [plus supplemental information]. United States. https://doi.org/10.1128/msystems.00534-20
Mageeney, Catherine M., Mohammed, Hamidu T., Dies, Marta, Anbari, Samira, Cudkevich, Netta, Chen, Yanyan, Buceta, Javier, and Ware, Vassie C. Tue .
"Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack [plus supplemental information]". United States. https://doi.org/10.1128/msystems.00534-20. https://www.osti.gov/servlets/purl/1668358.
@article{osti_1668358,
title = {Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack [plus supplemental information]},
author = {Mageeney, Catherine M. and Mohammed, Hamidu T. and Dies, Marta and Anbari, Samira and Cudkevich, Netta and Chen, Yanyan and Buceta, Javier and Ware, Vassie C.},
abstractNote = {A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc2155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analyses, phage plating efficiency experiments, microscopy, and immunoprecipitation assays, we show that Butters genes located in the central region of the genome play a key role in the defense against heterotypic viral attack. Our study suggests that a two-component system, articulated by interactions between protein products of genes 30 and 31, confers defense against heterotypic phage infection by PurpleHaze (cluster A/subcluster A3) or Alma (cluster A/subcluster A9) but is insufficient to confer defense against attack by the heterotypic phage Island3 (cluster I/subcluster I1). Therefore, based on heterotypic phage plating efficiencies on the Butters lysogen, additional prophage genes required for defense are implicated and further show specificity of prophage-encoded defense systems. IMPORTANCE: Many sequenced bacterial genomes, including those of pathogenic bacteria, contain prophages. Some prophages encode defense systems that protect their bacterial host against heterotypic viral attack. Understanding the mechanisms undergirding these defense systems is crucial to appreciate the scope of bacterial immunity against viral infections and will be critical for better implementation of phage therapy that would require evasion of these defenses. Furthermore, such knowledge of prophage-encoded defense mechanisms may be useful for developing novel genetic tools for engineering phage-resistant bacteria of industrial importance.},
doi = {10.1128/msystems.00534-20},
journal = {mSystems},
number = 5,
volume = 5,
place = {United States},
year = {Tue Oct 06 00:00:00 EDT 2020},
month = {Tue Oct 06 00:00:00 EDT 2020}
}
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