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Title: A nitrogenase-like enzyme system catalyzes methionine, ethylene, and methane biogenesis

Abstract

Bacterial production of hydrocarbon gases like ethylene and methane affects soil ecosystems and the climate. This study reveals that methane and ethylene from land and freshwater bacteria are directly produced by a previously unknown methionine biosynthesis pathway. This pathway employs a nitrogenase-like reductase that is distinct from known nitrogenases and nitrogenase-like reductases. The nitrogenase-like methylthio-alkane reductase specifically functions in carbon-sulfur bond breakage to reduce ubiquitous and significant volatile organic sulfur compounds such as dimethyl sulfide and (2-methylthio)ethanol. The liberated methanethiol serves as the immediate precursor to methionine. Whereas ethylene or methane is released into the environment. This pathway provides a biological route to the synthesis of ethylene in the absence of oxygen. Finally, anaerobic ethylene production by this pathway apparently explains the long-standing observation of ethylene accumulation in oxygen-depleted soils Methane production reveals an additional bacterial pathway that is distinct from archaeal methanogenesis.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [4]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [1]
  1. The Ohio State Univ., Columbus, OH (United States)
  2. Colorado State Univ., Fort Collins, CO (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
  4. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
The Ohio State Univ., Columbus, OH (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); NCI
Contributing Org.:
University of Colorado Cancer Center’s Genomics and Microarray Core Shared Resource; University of Colorado Boulder Research Computing Group; Environmental Molecular Sciences Laboratory; Battelle and UT-Battelle, LLC
OSTI Identifier:
1658249
Grant/Contract Number:  
SC0019338; AC05-76RLO1830; P30CA046934
Resource Type:
Accepted Manuscript
Journal Name:
Science (Online)
Additional Journal Information:
Journal Name: Science (Online); Journal Volume: 369; Journal Issue: 6507; Related Information: All raw mass spectraand searched data files for the proteome measurements have beendeposited into the ProteomeXchange repository: MassIVE accessionnumber MSV000084455 (FTP link to files: ftp://massive.ucsd.edu/MSV000084455/) and ProteomeXchange accession numberPXD015818. Raw transcriptomics reads are available in the NCBISequence Read Archive under accession numbers SRR10887327through SRR10887338 and BioProject accession number PRJNA601207.; Journal ID: ISSN 1095-9203
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; 10 SYNTHETIC FUELS; Nitrogenase; ethylene; methane; dimethyl sulfide; soil; sulfur; bacteria

Citation Formats

North, Justin A, Narrowe, Adrienne B, Xiong, Weili, Byerly, Kathryn M, Zhao, Guanqi, Young, Sarah J, Murali, Srividya, Wildenthal, John A, Cannon, William R, Wrighton, Kelly C, Hettich, Robert L, and Tabita, Fred Robert. A nitrogenase-like enzyme system catalyzes methionine, ethylene, and methane biogenesis. United States: N. p., 2020. Web. https://doi.org/10.1126/science.abb6310.
North, Justin A, Narrowe, Adrienne B, Xiong, Weili, Byerly, Kathryn M, Zhao, Guanqi, Young, Sarah J, Murali, Srividya, Wildenthal, John A, Cannon, William R, Wrighton, Kelly C, Hettich, Robert L, & Tabita, Fred Robert. A nitrogenase-like enzyme system catalyzes methionine, ethylene, and methane biogenesis. United States. https://doi.org/10.1126/science.abb6310
North, Justin A, Narrowe, Adrienne B, Xiong, Weili, Byerly, Kathryn M, Zhao, Guanqi, Young, Sarah J, Murali, Srividya, Wildenthal, John A, Cannon, William R, Wrighton, Kelly C, Hettich, Robert L, and Tabita, Fred Robert. Fri . "A nitrogenase-like enzyme system catalyzes methionine, ethylene, and methane biogenesis". United States. https://doi.org/10.1126/science.abb6310.
@article{osti_1658249,
title = {A nitrogenase-like enzyme system catalyzes methionine, ethylene, and methane biogenesis},
author = {North, Justin A and Narrowe, Adrienne B and Xiong, Weili and Byerly, Kathryn M and Zhao, Guanqi and Young, Sarah J and Murali, Srividya and Wildenthal, John A and Cannon, William R and Wrighton, Kelly C and Hettich, Robert L and Tabita, Fred Robert},
abstractNote = {Bacterial production of hydrocarbon gases like ethylene and methane affects soil ecosystems and the climate. This study reveals that methane and ethylene from land and freshwater bacteria are directly produced by a previously unknown methionine biosynthesis pathway. This pathway employs a nitrogenase-like reductase that is distinct from known nitrogenases and nitrogenase-like reductases. The nitrogenase-like methylthio-alkane reductase specifically functions in carbon-sulfur bond breakage to reduce ubiquitous and significant volatile organic sulfur compounds such as dimethyl sulfide and (2-methylthio)ethanol. The liberated methanethiol serves as the immediate precursor to methionine. Whereas ethylene or methane is released into the environment. This pathway provides a biological route to the synthesis of ethylene in the absence of oxygen. Finally, anaerobic ethylene production by this pathway apparently explains the long-standing observation of ethylene accumulation in oxygen-depleted soils Methane production reveals an additional bacterial pathway that is distinct from archaeal methanogenesis.},
doi = {10.1126/science.abb6310},
journal = {Science (Online)},
number = 6507,
volume = 369,
place = {United States},
year = {2020},
month = {8}
}

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This content will become publicly available on August 28, 2021
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