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Title: Zonal Soil Type Determines Soil Microbial Responses to Maize Cropping and Fertilization

Abstract

Microbial communities are essential drivers of soil functional processes such as nitrification and heterotrophic respiration. Although there is initial evidence revealing the importance of soil type in shaping microbial communities, there has been no in-depth, comprehensive survey to robustly establish it as a major determinant of microbial community composition, functional gene structure, or ecosystem functioning. We examined bacterial and fungal community structures using Illumina sequencing, microbial functional genes using GeoChip, microbial biomass using phospholipid fatty acid analysis, as well as functional processes of soil nitrification potential and CO2 efflux. We demonstrated the critical role of soil type in determining microbial responses to land use changes at the continental level. Our findings underscore the inherent difficulty in generalizing ecosystem responses across landscapes and suggest that assessments of community feedback must take soil types into consideration. Soil types heavily influence ecological dynamics. It remains controversial to what extent soil types shape microbial responses to land management changes, largely due to lack of in-depth comparison across various soil types. Here, we collected samples from three major zonal soil types spanning from cold temperate to subtropical climate zones. We examined bacterial and fungal community structures, as well as microbial functional genes. Different soil typesmore » had distinct microbial biomass levels and community compositions. Five years of maize cropping (growing corn or maize) changed the bacterial community composition of the Ultisol soil type and the fungal composition of the Mollisol soil type but had little effect on the microbial composition of the Inceptisol soil type. Meanwhile, 5 years of fertilization resulted in soil acidification. Microbial compositions of the Mollisol and Ultisol, but not the Inceptisol, were changed and correlated (P < 0.05) with soil pH. These results demonstrated the critical role of soil type in determining microbial responses to land management changes. We also found that soil nitrification potentials correlated with the total abundance of nitrifiers and that soil heterotrophic respiration correlated with the total abundance of carbon degradation genes, suggesting that changes in microbial community structure had altered ecosystem processes. Microbial communities are essential drivers of soil functional processes such as nitrification and heterotrophic respiration. Although there is initial evidence revealing the importance of soil type in shaping microbial communities, there has been no in-depth, comprehensive survey to robustly establish it as a major determinant of microbial community composition, functional gene structure, or ecosystem functioning. We examined bacterial and fungal community structures using Illumina sequencing, microbial functional genes using GeoChip, microbial biomass using phospholipid fatty acid analysis, as well as functional processes of soil nitrification potential and CO2 efflux. We demonstrated the critical role of soil type in determining microbial responses to land use changes at the continental level. Our findings underscore the inherent difficulty in generalizing ecosystem responses across landscapes and suggest that assessments of community feedback must take soil types into consideration.« less

Authors:
 [1];  [2];  [1];  [1];  [3];  [4];  [1];  [2]; ORCiD logo [5];  [6]; ORCiD logo [1]
  1. Tsinghua Univ., Beijing (China)
  2. Chinese Academy of Sciences (CAS), Nanjing (China)
  3. Chinese Academy of Sciences (CAS), Nanjing (China); Ningbo Academy of Agricultural Sciences (China)
  4. Univ. of Oklahoma, Norman, OK (United States); Guangdong Ocean Univ., Zhanjiang (China)
  5. Univ. of Oklahoma, Norman, OK (United States)
  6. Tsinghua Univ., Beijing (China); Univ. of Oklahoma, Norman, OK (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); Chinese Academy of Sciences (CAS); National Natural Science Foundation of China (NSFC)
OSTI Identifier:
1630596
Grant/Contract Number:  
AC02-05CH11231; XDB15010102; 2013ZX07315-001-03; 41471202; XDB15030200; KFJ-SW-STS-142; 41271258; 41430856
Resource Type:
Accepted Manuscript
Journal Name:
mSystems
Additional Journal Information:
Journal Volume: 1; Journal Issue: 4; Journal ID: ISSN 2379-5077
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; zonal soil type; microbial community; fertilization; soil functional process; GeoChip

Citation Formats

Zhao, Mengxin, Sun, Bo, Wu, Linwei, Gao, Qun, Wang, Feng, Wen, Chongqing, Wang, Mengmeng, Liang, Yuting, Hale, Lauren, Zhou, Jizhong, and Yang, Yunfeng. Zonal Soil Type Determines Soil Microbial Responses to Maize Cropping and Fertilization. United States: N. p., 2016. Web. https://doi.org/10.1128/msystems.00075-16.
Zhao, Mengxin, Sun, Bo, Wu, Linwei, Gao, Qun, Wang, Feng, Wen, Chongqing, Wang, Mengmeng, Liang, Yuting, Hale, Lauren, Zhou, Jizhong, & Yang, Yunfeng. Zonal Soil Type Determines Soil Microbial Responses to Maize Cropping and Fertilization. United States. https://doi.org/10.1128/msystems.00075-16
Zhao, Mengxin, Sun, Bo, Wu, Linwei, Gao, Qun, Wang, Feng, Wen, Chongqing, Wang, Mengmeng, Liang, Yuting, Hale, Lauren, Zhou, Jizhong, and Yang, Yunfeng. Tue . "Zonal Soil Type Determines Soil Microbial Responses to Maize Cropping and Fertilization". United States. https://doi.org/10.1128/msystems.00075-16. https://www.osti.gov/servlets/purl/1630596.
@article{osti_1630596,
title = {Zonal Soil Type Determines Soil Microbial Responses to Maize Cropping and Fertilization},
author = {Zhao, Mengxin and Sun, Bo and Wu, Linwei and Gao, Qun and Wang, Feng and Wen, Chongqing and Wang, Mengmeng and Liang, Yuting and Hale, Lauren and Zhou, Jizhong and Yang, Yunfeng},
abstractNote = {Microbial communities are essential drivers of soil functional processes such as nitrification and heterotrophic respiration. Although there is initial evidence revealing the importance of soil type in shaping microbial communities, there has been no in-depth, comprehensive survey to robustly establish it as a major determinant of microbial community composition, functional gene structure, or ecosystem functioning. We examined bacterial and fungal community structures using Illumina sequencing, microbial functional genes using GeoChip, microbial biomass using phospholipid fatty acid analysis, as well as functional processes of soil nitrification potential and CO2 efflux. We demonstrated the critical role of soil type in determining microbial responses to land use changes at the continental level. Our findings underscore the inherent difficulty in generalizing ecosystem responses across landscapes and suggest that assessments of community feedback must take soil types into consideration. Soil types heavily influence ecological dynamics. It remains controversial to what extent soil types shape microbial responses to land management changes, largely due to lack of in-depth comparison across various soil types. Here, we collected samples from three major zonal soil types spanning from cold temperate to subtropical climate zones. We examined bacterial and fungal community structures, as well as microbial functional genes. Different soil types had distinct microbial biomass levels and community compositions. Five years of maize cropping (growing corn or maize) changed the bacterial community composition of the Ultisol soil type and the fungal composition of the Mollisol soil type but had little effect on the microbial composition of the Inceptisol soil type. Meanwhile, 5 years of fertilization resulted in soil acidification. Microbial compositions of the Mollisol and Ultisol, but not the Inceptisol, were changed and correlated (P < 0.05) with soil pH. These results demonstrated the critical role of soil type in determining microbial responses to land management changes. We also found that soil nitrification potentials correlated with the total abundance of nitrifiers and that soil heterotrophic respiration correlated with the total abundance of carbon degradation genes, suggesting that changes in microbial community structure had altered ecosystem processes. Microbial communities are essential drivers of soil functional processes such as nitrification and heterotrophic respiration. Although there is initial evidence revealing the importance of soil type in shaping microbial communities, there has been no in-depth, comprehensive survey to robustly establish it as a major determinant of microbial community composition, functional gene structure, or ecosystem functioning. We examined bacterial and fungal community structures using Illumina sequencing, microbial functional genes using GeoChip, microbial biomass using phospholipid fatty acid analysis, as well as functional processes of soil nitrification potential and CO2 efflux. We demonstrated the critical role of soil type in determining microbial responses to land use changes at the continental level. Our findings underscore the inherent difficulty in generalizing ecosystem responses across landscapes and suggest that assessments of community feedback must take soil types into consideration.},
doi = {10.1128/msystems.00075-16},
journal = {mSystems},
number = 4,
volume = 1,
place = {United States},
year = {2016},
month = {7}
}

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Cited by: 18 works
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Figures / Tables:

FIG 1 FIG 1: Boxplots of βNTI distribution in the Mollisol (A), Inceptisol (B), and Ultisol (C) soil types. Each boxplot shows the median value (thick black line), first quartile (bottom of the box), third quartile (top of the box), and range of the data that were no more than 1.5 timesmore » height of the boxes (error bars). Moderate outliers (circles) and extreme outlier (asterisk) are also shown. Significance was determined by one-way ANOVA followed by the LSD test. Boxes with the same letter were not statistically significantly different (P > 0.05). Boxes of within groups represent pairwise comparisons between any two samples within a treatment. Boxes for Nm-N, Cm-C, and Sm-S represent pairwise comparisons between maize cropping samples (m suffix) and bare fallow samples (no suffix) for samples from sites N, C, and S. Boxes of Nf-Nm, Cf-Cm, and Sf-Sm represent pairwise comparisons between NPK fertilization samples (f suffix) and maize cropping samples (m suffix) from the three sites.« less

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