Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Opto-Electric Cellular Biosensor Using Optically Transparent Indium Tin Oxide (ITO) Electrodes

Abstract

Indium tin oxide (ITO) biosensors are used to perform simultaneous optical and electrical measurements in order to examine the dynamic cellular attachment, spreading, and proliferation of endothelial cells (ECs) as well as cytotoxic effects when exposed to cytochalasin D. A detailed description of the fabrication of these sensors is provided and their superior optical characteristics are qualitatively shown using four different microscopic images. Differential interference contrast microscopy (DICM) images were acquired simultaneously with micro-impedance measurements as a function of frequency and time. A digital image processing algorithm quantified the cell-covered electrode area as a function of time. In addition, cytotoxicity effects, produced by the toxic agent cytochalasin D, were examined using micro-impedance measurements, confocal microscopy images of stained actin-filaments, and interference reflection contrast microscopy (IRCM) capable of examining the bottom morphology of a cell. The results of this study show (1) the dynamic optical and electrical cellular characteristics using optically thin ITO biosensors; (2) qualitative agreement between cell-covered electrode area and electrical impedance during cellular attachment; (3) in vitro cytotoxicity detection of ECs due to 3 µM cytochalasin D. The present opto-electric biosensor system is unique in that a simultaneous and integrated cellular analysis is possible for a variety ofmore » living cells.« less

Authors:
 [1];  [2];  [3];  [2];  [3];  [2]
+ Show Author Affiliations
  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Mechanical, Aerospace and Biomedical Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  2. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Mechanical, Aerospace and Biomedical Engineering
  3. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Materials Science and Engineering
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1628564
Resource Type:
Accepted Manuscript
Journal Name:
Sensors
Additional Journal Information:
Journal Volume: 8; Journal Issue: 5; Journal ID: ISSN 1424-8220
Publisher:
MDPI AG
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 47 OTHER INSTRUMENTATION; 42 ENGINEERING; Chemistry; Engineering; Instruments & Instrumentation

Citation Formats

Choi, Chang, Margraves, Chuck, Jun, Seung, English, Anthony, Rack, Philip, and Kihm, Kenneth. Opto-Electric Cellular Biosensor Using Optically Transparent Indium Tin Oxide (ITO) Electrodes. United States: N. p., 2008. Web. doi:10.3390/s8053257.
Copy to clipboard
Choi, Chang, Margraves, Chuck, Jun, Seung, English, Anthony, Rack, Philip, & Kihm, Kenneth. Opto-Electric Cellular Biosensor Using Optically Transparent Indium Tin Oxide (ITO) Electrodes. United States. https://doi.org/10.3390/s8053257
Copy to clipboard
Choi, Chang, Margraves, Chuck, Jun, Seung, English, Anthony, Rack, Philip, and Kihm, Kenneth. Thu . "Opto-Electric Cellular Biosensor Using Optically Transparent Indium Tin Oxide (ITO) Electrodes". United States. https://doi.org/10.3390/s8053257. https://www.osti.gov/servlets/purl/1628564.
Copy to clipboard
@article{osti_1628564,
title = {Opto-Electric Cellular Biosensor Using Optically Transparent Indium Tin Oxide (ITO) Electrodes},
author = {Choi, Chang and Margraves, Chuck and Jun, Seung and English, Anthony and Rack, Philip and Kihm, Kenneth},
abstractNote = {Indium tin oxide (ITO) biosensors are used to perform simultaneous optical and electrical measurements in order to examine the dynamic cellular attachment, spreading, and proliferation of endothelial cells (ECs) as well as cytotoxic effects when exposed to cytochalasin D. A detailed description of the fabrication of these sensors is provided and their superior optical characteristics are qualitatively shown using four different microscopic images. Differential interference contrast microscopy (DICM) images were acquired simultaneously with micro-impedance measurements as a function of frequency and time. A digital image processing algorithm quantified the cell-covered electrode area as a function of time. In addition, cytotoxicity effects, produced by the toxic agent cytochalasin D, were examined using micro-impedance measurements, confocal microscopy images of stained actin-filaments, and interference reflection contrast microscopy (IRCM) capable of examining the bottom morphology of a cell. The results of this study show (1) the dynamic optical and electrical cellular characteristics using optically thin ITO biosensors; (2) qualitative agreement between cell-covered electrode area and electrical impedance during cellular attachment; (3) in vitro cytotoxicity detection of ECs due to 3 µM cytochalasin D. The present opto-electric biosensor system is unique in that a simultaneous and integrated cellular analysis is possible for a variety of living cells.},
doi = {10.3390/s8053257},
journal = {Sensors},
number = 5,
volume = 8,
place = {United States},
year = {Thu May 01 00:00:00 EDT 2008},
month = {Thu May 01 00:00:00 EDT 2008}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.3390/s8053257
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Fractal motion of mammalian cells
journal, September 1989

An endothelial cell compatible biosensor fabricated using optically thin indium tin oxide silicon nitride electrodes
journal, May 2007

  • Choi, Chang K.; English, Anthony E.; Jun, Seung-Ik
  • Biosensors and Bioelectronics, Vol. 22, Issue 11
  • DOI: 10.1016/j.bios.2006.10.006

Monitoring fibroblast behavior in tissue culture with an applied electric field.
journal, June 1984

  • Giaever, I.; Keese, C. R.
  • Proceedings of the National Academy of Sciences, Vol. 81, Issue 12
  • DOI: 10.1073/pnas.81.12.3761

Spatial variations in endothelial barrier function in disturbed flows in vitro
journal, February 2000

Transparent indium-tin oxide electrode patterns for extracellular, multisite recording in neuronal cultures
journal, November 1985

Electric Cell–Substrate Impedance Sensing (ECIS) as a Noninvasive Means to Monitor the Kinetics of Cell Spreading to Artificial Surfaces
journal, August 2000

  • Wegener, Joachim; Keese, Charles R.; Giaever, Ivar
  • Experimental Cell Research, Vol. 259, Issue 1
  • DOI: 10.1006/excr.2000.4919

Transparent indium-tin oxide electrode patterns for extracellular, multisite recording in neuronal cultures
journal, November 1985

Growth of cultured RPE and endothelial cells is inhibited by blue light but not green or red light
journal, January 1990

  • Dorey, C. Kathleen; Delori, Francois C.; Akeo, Kiyoshi
  • Current Eye Research, Vol. 9, Issue 6
  • DOI: 10.3109/02713689008999595

Human substance P receptor lacking the C-terminal domain remains competent to desensitize and internalize: Truncated human SP receptor desensitization
journal, January 2003

Use of Electric Fields to Monitor the Dynamical Aspect of Cell Behavior in Tissue Culture
journal, February 1986

  • Giaever, Ivar; Keese, Charles R.
  • IEEE Transactions on Biomedical Engineering, Vol. BME-33, Issue 2
  • DOI: 10.1109/tbme.1986.325896

Assessment of Cytotoxicity Using Electric Cell−Substrate Impedance Sensing:  Concentration and Time Response Function Approach
journal, November 2002

  • Xiao, Caide; Lachance, Bernard; Sunahara, Geoffrey
  • Analytical Chemistry, Vol. 74, Issue 22
  • DOI: 10.1021/ac025848f

Micromotion of mammalian cells measured electrically.
journal, September 1991

  • Giaever, I.; Keese, C. R.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 17
  • DOI: 10.1073/pnas.88.17.7896

Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.
journal, September 1992

  • Tiruppathi, C.; Malik, A. B.; Del Vecchio, P. J.
  • Proceedings of the National Academy of Sciences, Vol. 89, Issue 17
  • DOI: 10.1073/pnas.89.17.7919

A statistical parameter study of indium tin oxide thin films deposited by radio-frequency sputtering
journal, April 2005

  • Jun, Seung-Ik; McKnight, Timothy E.; Simpson, Michael L.
  • Thin Solid Films, Vol. 476, Issue 1
  • DOI: 10.1016/j.tsf.2004.09.011

Optoelectric biosensor using indium-tin-oxide electrodes
journal, January 2007

  • Choi, Chang Kyoung; Kihm, Kenneth D.; English, Anthony E.
  • Optics Letters, Vol. 32, Issue 11
  • DOI: 10.1364/OL.32.001405

On-Line Monitoring of Cell Growth and Cytotoxicity Using Electric Cell-Substrate Impedance Sensing (ECIS)
journal, June 2003

  • Xiao, C.; Luong, J. H. T.
  • Biotechnology Progress, Vol. 19, Issue 3
  • DOI: 10.1021/bp025733x

Assessment of Cytotoxicity Using Electric Cell−Substrate Impedance Sensing:  Concentration and Time Response Function Approach
journal, November 2002

  • Xiao, Caide; Lachance, Bernard; Sunahara, Geoffrey
  • Analytical Chemistry, Vol. 74, Issue 22
  • DOI: 10.1021/ac025848f

Electrical method for detection of endothelial cell shape change in real time: assessment of endothelial barrier function.
journal, September 1992

  • Tiruppathi, C.; Malik, A. B.; Del Vecchio, P. J.
  • Proceedings of the National Academy of Sciences, Vol. 89, Issue 17
  • DOI: 10.1073/pnas.89.17.7919

On-Line Monitoring of Cell Growth and Cytotoxicity Using Electric Cell-Substrate Impedance Sensing (ECIS)
journal, June 2003

  • Xiao, C.; Luong, J. H. T.
  • Biotechnology Progress, Vol. 19, Issue 3
  • DOI: 10.1021/bp025733x

Growth of cultured RPE and endothelial cells is inhibited by blue light but not green or red light
journal, January 1990

  • Dorey, C. Kathleen; Delori, Francois C.; Akeo, Kiyoshi
  • Current Eye Research, Vol. 9, Issue 6
  • DOI: 10.3109/02713689008999595

Micromotion of mammalian cells measured electrically.
journal, September 1991

  • Giaever, I.; Keese, C. R.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 17
  • DOI: 10.1073/pnas.88.17.7896

Electric Cell–Substrate Impedance Sensing (ECIS) as a Noninvasive Means to Monitor the Kinetics of Cell Spreading to Artificial Surfaces
journal, August 2000

  • Wegener, Joachim; Keese, Charles R.; Giaever, Ivar
  • Experimental Cell Research, Vol. 259, Issue 1
  • DOI: 10.1006/excr.2000.4919

An In-Depth Analysis of Electric Cell−Substrate Impedance Sensing To Study the Attachment and Spreading of Mammalian Cells
journal, March 2002

  • Xiao, Caide; Lachance, Bernard; Sunahara, Geoffrey
  • Analytical Chemistry, Vol. 74, Issue 6
  • DOI: 10.1021/ac011104a

An endothelial cell compatible biosensor fabricated using optically thin indium tin oxide silicon nitride electrodes
journal, May 2007

  • Choi, Chang K.; English, Anthony E.; Jun, Seung-Ik
  • Biosensors and Bioelectronics, Vol. 22, Issue 11
  • DOI: 10.1016/j.bios.2006.10.006

Neutrophil transendothelial migration is independent of tight junctions and occurs preferentially at tricellular corners.
journal, September 1997

    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    Electrical and optical properties of sputtered ultra-thin indium tin oxide films using xenon/argon gas
    journal, March 2019

    • Hamouda, F.; Herth, E.; David, C.
    • Journal of Materials Science: Materials in Electronics, Vol. 30, Issue 9
    • DOI: 10.1007/s10854-019-01171-w

    Spatially resolved electrical impedance methods for cell and particle characterization
    journal, November 2019

    • Schwarz, Marvin; Jendrusch, Michael; Constantinou, Iordania
    • ELECTROPHORESIS, Vol. 41, Issue 1-2
    • DOI: 10.1002/elps.201900286

    Organosilane-functionalization of nanostructured indium tin oxide films
    journal, December 2016

      Sort by:
      [ × clear filter / sort ]

      Similar Records in DOE PAGES and OSTI.GOV collections: