Impact of DNA damaging agents on genome-wide transcriptional profiles in two marine Synechococcus species
Abstract
Marine microorganisms, particularly those residing in coastal areas, may come in contact with any number of chemicals of environmental or xenobiotic origin. The sensitivity and response of marine cyanobacteria to such chemicals is, at present, poorly understood. We have looked at the transcriptional response of well characterized Synechococcus open ocean (WH8102) and coastal (CC9311) isolates to two DNA damaging agents, mitomycin C and ethidium bromide, using whole-genome expression microarrays. The coastal strain showed differential regulation of a larger proportion of its genome following “shock” treatment with each agent. Many of the orthologous genes in these strains, including those encoding sensor kinases, showed different transcriptional responses, with the CC9311 genes more likely to show significant changes in both treatments. While the overall response of each strain was considerably different, there were distinct transcriptional responses common to both strains observed for each DNA damaging agent, linked to the mode of action of each chemical. In both CC9311 and WH8102 there was evidence of SOS response induction under mitomycin C treatment, with genes recA, lexA and umuC significantly upregulated in this experiment but not under ethidium bromide treatment. Conversely, ethidium bromide treatment tended to result in upregulation of the DNA-directed RNA polymerase genes,more »
- Authors:
-
- Macquarie Univ., NSW (Australia). Dept. of Chemistry and Biomolecular Sciences
- J. Craig Venter Inst., Inc., Rockville, MD (United States). Microbial and Environmental Genomics Dept.
- Univ. of California, San Diego, CA (United States). Scripps Inst. of Oceanography. Marine Biology Research Division
- Univ. of California, San Diego, CA (United States). Scripps Inst. of Oceanography. Marine Biology Research Division; J. Craig Venter Inst., Inc., San Diego, CA (United States). Microbial and Environmental Genomics Dept.
- Publication Date:
- Research Org.:
- Univ. of California, San Diego, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1628092
- Grant/Contract Number:
- FG03-01ER63148
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 4; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology; cyanobacteria; Synechococcus; transcriptome; microarray; toxic stress; ethidium bromide; mitomycin C; DNA damage
Citation Formats
Tetu, Sasha G., Johnson, Daniel A., Varkey, Deepa, Phillippy, Katherine, Stuart, Rhona K., Dupont, Chris L., Hassan, Karl A., Palenik, Brian, and Paulsen, Ian T. Impact of DNA damaging agents on genome-wide transcriptional profiles in two marine Synechococcus species. United States: N. p., 2013.
Web. doi:10.3389/fmicb.2013.00232.
Tetu, Sasha G., Johnson, Daniel A., Varkey, Deepa, Phillippy, Katherine, Stuart, Rhona K., Dupont, Chris L., Hassan, Karl A., Palenik, Brian, & Paulsen, Ian T. Impact of DNA damaging agents on genome-wide transcriptional profiles in two marine Synechococcus species. United States. https://doi.org/10.3389/fmicb.2013.00232
Tetu, Sasha G., Johnson, Daniel A., Varkey, Deepa, Phillippy, Katherine, Stuart, Rhona K., Dupont, Chris L., Hassan, Karl A., Palenik, Brian, and Paulsen, Ian T. Tue .
"Impact of DNA damaging agents on genome-wide transcriptional profiles in two marine Synechococcus species". United States. https://doi.org/10.3389/fmicb.2013.00232. https://www.osti.gov/servlets/purl/1628092.
@article{osti_1628092,
title = {Impact of DNA damaging agents on genome-wide transcriptional profiles in two marine Synechococcus species},
author = {Tetu, Sasha G. and Johnson, Daniel A. and Varkey, Deepa and Phillippy, Katherine and Stuart, Rhona K. and Dupont, Chris L. and Hassan, Karl A. and Palenik, Brian and Paulsen, Ian T.},
abstractNote = {Marine microorganisms, particularly those residing in coastal areas, may come in contact with any number of chemicals of environmental or xenobiotic origin. The sensitivity and response of marine cyanobacteria to such chemicals is, at present, poorly understood. We have looked at the transcriptional response of well characterized Synechococcus open ocean (WH8102) and coastal (CC9311) isolates to two DNA damaging agents, mitomycin C and ethidium bromide, using whole-genome expression microarrays. The coastal strain showed differential regulation of a larger proportion of its genome following “shock” treatment with each agent. Many of the orthologous genes in these strains, including those encoding sensor kinases, showed different transcriptional responses, with the CC9311 genes more likely to show significant changes in both treatments. While the overall response of each strain was considerably different, there were distinct transcriptional responses common to both strains observed for each DNA damaging agent, linked to the mode of action of each chemical. In both CC9311 and WH8102 there was evidence of SOS response induction under mitomycin C treatment, with genes recA, lexA and umuC significantly upregulated in this experiment but not under ethidium bromide treatment. Conversely, ethidium bromide treatment tended to result in upregulation of the DNA-directed RNA polymerase genes, not observed following mitomycin C treatment. Interestingly, a large number of genes residing on putative genomic island regions of eac},
doi = {10.3389/fmicb.2013.00232},
journal = {Frontiers in Microbiology},
number = ,
volume = 4,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2013},
month = {Tue Jan 01 00:00:00 EST 2013}
}
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