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Title: Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport

Abstract

A significant fraction of a plant’s nuclear genome encodes chloroplast-targeted proteins, many of which are devoted to the assembly and function of the photosynthetic apparatus. Using digital video imaging of chlorophyll fluorescence, we isolated proton gradient regulation 7 (pgr7) as an Arabidopsis thaliana mutant with low nonphotochemical quenching of chlorophyll fluorescence (NPQ). In pgr7, the xanthophyll cycle and the PSBS gene product, previously identified NPQ factors, were still functional, but the efficiency of photosynthetic electron transport was lower than in the wild type. The pgr7 mutant was also smaller in size and had lower chlorophyll content than the wild type in optimal growth conditions. Positional cloning located the pgr7 mutation in the At3g21200 (PGR7) gene, which was predicted to encode a chloroplast protein of unknown function. Chloroplast targeting of PGR7 was confirmed by transient expression of a GFP fusion protein and by stable expression and subcellular localization of an epitope-tagged version of PGR7. Bioinformatic analyses revealed that the PGR7 protein has two domains that are conserved in plants, algae, and bacteria, and the N-terminal domain is predicted to bind a cofactor such as FMN. Thus, we identified PGR7 as a novel, conserved nuclear gene that is necessary for efficient photosyntheticmore » electron transport in chloroplasts of Arabidopsis.« less

Authors:
 [1];  [2];  [1];  [3];  [4];  [4];  [1];  [5];  [6]
+ Show Author Affiliations
  1. University of California, Berkeley, CA (United States)
  2. Kyushu University, Fukuoka (Japan); Kyoto University (Japan)
  3. University of California, Berkeley, CA (United States). Dept. of Bioengineering
  4. Colorado State University, Fort Collins, CO (United States)
  5. Kyoto University (Japan)
  6. University of California, Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDA; National Science Foundation (NSF); Japan Society for the Promotion of Science (JSPS)
OSTI Identifier:
1627416
Grant/Contract Number:  
AC02-05CH11231; 98-35306-6600; 449A449B; IBN-0418993; IOS-0847442; 0732065; 19-8015; 17GS0316
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 5; Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; chloroplasts; chlorophyll; fluorescence imaging; fluorescence; light; Arabidopsis thaliana; photosynthesis; phylogenetic analysis

Citation Formats

Jung, Hou-Sung, Okegawa, Yuki, Shih, Patrick M., Kellogg, Elizabeth, Abdel-Ghany, Salah E., Pilon, Marinus, Sjölander, Kimmen, Shikanai, Toshiharu, and Niyogi, Krishna K. Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport. United States: N. p., 2010. Web. doi:10.1371/journal.pone.0011688.
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Jung, Hou-Sung, Okegawa, Yuki, Shih, Patrick M., Kellogg, Elizabeth, Abdel-Ghany, Salah E., Pilon, Marinus, Sjölander, Kimmen, Shikanai, Toshiharu, & Niyogi, Krishna K. Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport. United States. https://doi.org/10.1371/journal.pone.0011688
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Jung, Hou-Sung, Okegawa, Yuki, Shih, Patrick M., Kellogg, Elizabeth, Abdel-Ghany, Salah E., Pilon, Marinus, Sjölander, Kimmen, Shikanai, Toshiharu, and Niyogi, Krishna K. Wed . "Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport". United States. https://doi.org/10.1371/journal.pone.0011688. https://www.osti.gov/servlets/purl/1627416.
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@article{osti_1627416,
title = {Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport},
author = {Jung, Hou-Sung and Okegawa, Yuki and Shih, Patrick M. and Kellogg, Elizabeth and Abdel-Ghany, Salah E. and Pilon, Marinus and Sjölander, Kimmen and Shikanai, Toshiharu and Niyogi, Krishna K.},
abstractNote = {A significant fraction of a plant’s nuclear genome encodes chloroplast-targeted proteins, many of which are devoted to the assembly and function of the photosynthetic apparatus. Using digital video imaging of chlorophyll fluorescence, we isolated proton gradient regulation 7 (pgr7) as an Arabidopsis thaliana mutant with low nonphotochemical quenching of chlorophyll fluorescence (NPQ). In pgr7, the xanthophyll cycle and the PSBS gene product, previously identified NPQ factors, were still functional, but the efficiency of photosynthetic electron transport was lower than in the wild type. The pgr7 mutant was also smaller in size and had lower chlorophyll content than the wild type in optimal growth conditions. Positional cloning located the pgr7 mutation in the At3g21200 (PGR7) gene, which was predicted to encode a chloroplast protein of unknown function. Chloroplast targeting of PGR7 was confirmed by transient expression of a GFP fusion protein and by stable expression and subcellular localization of an epitope-tagged version of PGR7. Bioinformatic analyses revealed that the PGR7 protein has two domains that are conserved in plants, algae, and bacteria, and the N-terminal domain is predicted to bind a cofactor such as FMN. Thus, we identified PGR7 as a novel, conserved nuclear gene that is necessary for efficient photosynthetic electron transport in chloroplasts of Arabidopsis.},
doi = {10.1371/journal.pone.0011688},
journal = {PLoS ONE},
number = 7,
volume = 5,
place = {United States},
year = {Wed Jul 21 00:00:00 EDT 2010},
month = {Wed Jul 21 00:00:00 EDT 2010}
}
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https://doi.org/10.1371/journal.pone.0011688
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Iron-Sulfur Cluster Biogenesis in Chloroplasts. Involvement of the Scaffold Protein CpIscA
journal, May 2005

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Non-Photochemical Quenching. A Response to Excess Light Energy
journal, April 2001

  • Müller, Patricia; Li, Xiao-Ping; Niyogi, Krishna K.
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PAA1, a P-Type ATPase of Arabidopsis, Functions in Copper Transport in Chloroplasts
journal, May 2003

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Arabidopsis Mutants Define a Central Role for the Xanthophyll Cycle in the Regulation of Photosynthetic Energy Conversion
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Two P-Type ATPases Are Required for Copper Delivery in Arabidopsis thaliana Chloroplasts
journal, March 2005

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journal, August 2007

  • Brown, Duncan P.; Krishnamurthy, Nandini; Sjölander, Kimmen
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Measuring P700 Absorbance Changes around 830 nm with a New Type of Pulse Modulation System
journal, October 1988

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  • Zeitschrift für Naturforschung C, Vol. 43, Issue 9-10
  • DOI: 10.1515/znc-1988-9-1010
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