Structures of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus furiosus: Implications for the Targeting Step at the Membrane

Egea, Pascal F.; Tsuruta, Hiro; de Leon, Gladys P.; Napetschnig, Johanna; Walter, Peter; Stroud, Robert M.

doi:10.1371/journal.pone.0003619

Title: Structures of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus furiosus: Implications for the Targeting Step at the Membrane

Journal Article · 02 November 2008 · PLoS ONE

DOI: https://doi.org/10.1371/journal.pone.0003619 · OSTI ID:1627361

Egea, Pascal F. ^[1]; Tsuruta, Hiro ^[2]; de Leon, Gladys P. ^[3]; Napetschnig, Johanna ^[4]; Walter, Peter ^[3]; Stroud, Robert M. ^[3]

Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; DOE/OSTI
SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics
Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Rockefeller Univ., New York, NY (United States). Lab. of Cell Biology and Howard Hughes Medical Inst.

In all organisms, a ribonucleoprotein called the signal recognition particle (SRP) and its receptor (SR) target nascent proteins from the ribosome to the translocon for secretion or membrane insertion. We present the first X-ray structures of an archeal FtsY, the receptor from the hyper-thermophile Pyrococcus furiosus (Pfu), in its free and GDPNmagnesium-bound forms. The highly charged N-terminal domain of Pfu-FtsY is distinguished by a long N-terminal helix. The basic charges on the surface of this helix are likely to regulate interactions at the membrane. A peripheral GDP bound near a regulatory motif could indicate a site of interaction between the receptor and ribosomal or SRP RNAs. Small angle X-ray scattering and analytical ultracentrifugation indicate that the crystal structure of Pfu-FtsY correlates well with the average conformation in solution. Based on previous structures of two sub-complexes, we propose a model of the core of archeal and eukaryotic SRPNSR targeting complexes.

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Research Organization:: SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: National Center for Research Resources (NCRR); National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1627361

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 11 Vol. 3; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Structures of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus furiosus: Implications for the Targeting Step at the Membrane

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